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The map label for this gene is dapG1 [H]

Identifier: 218232221

GI number: 218232221

Start: 1726725

End: 1727954

Strand: Reverse

Name: dapG1 [H]

Synonym: BCB4264_A1821

Alternate gene names: 218232221

Gene position: 1727954-1726725 (Counterclockwise)

Preceding gene: 218235795

Following gene: 218234334

Centisome position: 31.89

GC content: 37.72

Gene sequence:

>1230_bases
ATGGAAACAATTGTTCAAAAATTTGGTGGCACTTCTGTCGGAAGCGTCGAACGCATTCAACATGTAGCAAATTTAATTAT
TGAAGAATATGAACGAGGTCATAGCATTGTCTCTGTCGTTTCAGCAATGGGAAAAAGCACAGATGAACTTGTCGCACTCG
CTAACGCTATCACAGAAAATCCAAGTAAACGTGAAATGGATATGCTTCTATCTACAGGAGAACAAGTAACTATTTCATTA
TTAACAATAGCACTGCAAGCAAAAGGCTATCATGCAATTTCATTAACAGGGTGGCAAGCAGGTATTACGACAGAATCTGT
ACATAGTAGTGCACGGATTACTGACATTCATACAGATCGTATTCAATCTTATCTTGCTGAAGGTACGATTGTTATTGTAG
CTGGTTTCCAAGGAGTCAGTGAAGATTTTGAAATTACAACGCTTGGACGAGGCGGTTCTGATACAACTGCTGTCGCATTA
GCCGCTGCACTTAAAGCAAAAAAATGTGATATTTATACGGATGTTACAGGCGTATATACGACGGACCCACGAGTTGTAAA
AGATGCTTACAAATTAGACGAAATTTCTTATGACGAAATGTTAGAGCTTGCTAATCTCGGTGCTGGCGTATTACACCCGC
GCGCCGTTGAGTTTGCTAAAAATCATAACGTAGTTTTAGAAGTTCGCTCAAGTATGGAACAAGAAAACGGAACAATTGTA
AAAGGAGAATGTAACATGGAACAACAATCAATCGTTAAAGGTATTGCATTTGAGGATAATATTACACGTGTCACAATTAA
AGGACTAGAACAAGGCTCGCTTTCAACAGTTTTCTCTACATTAGCAGCAGCACACATTAATGTAGATATCATCATTCAAA
GTATTACAAATGAAGGAACTGTACATCTCTCCTTCTCCATTCACTCGAATGATTTAAGAGAAACTTTAAAAGTTTTGGAA
CAAAATCAAGAAACTCTTCACTATGAATCTGTAGAACATGAAAACCACTTAGCAAAAGTATCAATCGTAGGATCTGGCAT
GGTATCTAACCCAGGTGTCGCTGCAAATATGTTCACTACTTTAAAAGAAGAAGATATTCATATTAAAATGGTAAGTACAT
CAGAAATTAAAGTGTCTGTCGTTATTGACCGCCTTCATTTAGTAACAGGCGTTGAGGCGCTGCATCAATCATTTATGGCG
AAAATTGAGCCTTTCGTGCAAATAAGCTAA

Upstream 100 bases:

>100_bases
AGATGGTGTTTTTTGTTTGTCTTTCTGGTAACTACAGGAGTATTGGGATTGATATATTTCCTAATCTATTCTTAAAAATA
GACGAAAGAAGGAATTTGTA

Downstream 100 bases:

>100_bases
TTTGATATGTTTAGTAACGCTTATAAAAAAATCATATTAATCTACTTCCTTCCTATTAAATAAAAAGCTATTCGTTAACA
AGATGCTATATGACATATTA

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase 2; Aspartokinase II [H]

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
METIVQKFGGTSVGSVERIQHVANLIIEEYERGHSIVSVVSAMGKSTDELVALANAITENPSKREMDMLLSTGEQVTISL
LTIALQAKGYHAISLTGWQAGITTESVHSSARITDIHTDRIQSYLAEGTIVIVAGFQGVSEDFEITTLGRGGSDTTAVAL
AAALKAKKCDIYTDVTGVYTTDPRVVKDAYKLDEISYDEMLELANLGAGVLHPRAVEFAKNHNVVLEVRSSMEQENGTIV
KGECNMEQQSIVKGIAFEDNITRVTIKGLEQGSLSTVFSTLAAAHINVDIIIQSITNEGTVHLSFSIHSNDLRETLKVLE
QNQETLHYESVEHENHLAKVSIVGSGMVSNPGVAANMFTTLKEEDIHIKMVSTSEIKVSVVIDRLHLVTGVEALHQSFMA
KIEPFVQIS

Sequences:

>Translated_409_residues
METIVQKFGGTSVGSVERIQHVANLIIEEYERGHSIVSVVSAMGKSTDELVALANAITENPSKREMDMLLSTGEQVTISL
LTIALQAKGYHAISLTGWQAGITTESVHSSARITDIHTDRIQSYLAEGTIVIVAGFQGVSEDFEITTLGRGGSDTTAVAL
AAALKAKKCDIYTDVTGVYTTDPRVVKDAYKLDEISYDEMLELANLGAGVLHPRAVEFAKNHNVVLEVRSSMEQENGTIV
KGECNMEQQSIVKGIAFEDNITRVTIKGLEQGSLSTVFSTLAAAHINVDIIIQSITNEGTVHLSFSIHSNDLRETLKVLE
QNQETLHYESVEHENHLAKVSIVGSGMVSNPGVAANMFTTLKEEDIHIKMVSTSEIKVSVVIDRLHLVTGVEALHQSFMA
KIEPFVQIS
>Mature_409_residues
METIVQKFGGTSVGSVERIQHVANLIIEEYERGHSIVSVVSAMGKSTDELVALANAITENPSKREMDMLLSTGEQVTISL
LTIALQAKGYHAISLTGWQAGITTESVHSSARITDIHTDRIQSYLAEGTIVIVAGFQGVSEDFEITTLGRGGSDTTAVAL
AAALKAKKCDIYTDVTGVYTTDPRVVKDAYKLDEISYDEMLELANLGAGVLHPRAVEFAKNHNVVLEVRSSMEQENGTIV
KGECNMEQQSIVKGIAFEDNITRVTIKGLEQGSLSTVFSTLAAAHINVDIIIQSITNEGTVHLSFSIHSNDLRETLKVLE
QNQETLHYESVEHENHLAKVSIVGSGMVSNPGVAANMFTTLKEEDIHIKMVSTSEIKVSVVIDRLHLVTGVEALHQSFMA
KIEPFVQIS

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=342, Percent_Identity=31.5789473684211, Blast_Score=147, Evalue=9e-37,
Organism=Escherichia coli, GI1790455, Length=256, Percent_Identity=37.890625, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1790376, Length=279, Percent_Identity=27.2401433691756, Blast_Score=84, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6320893, Length=377, Percent_Identity=27.3209549071618, Blast_Score=113, Evalue=5e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR005260
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 44404; Mature: 44404

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METIVQKFGGTSVGSVERIQHVANLIIEEYERGHSIVSVVSAMGKSTDELVALANAITEN
CCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHCC
PSKREMDMLLSTGEQVTISLLTIALQAKGYHAISLTGWQAGITTESVHSSARITDIHTDR
CCHHHHHHHHHCCCCEEEEEEEEEEECCCEEEEEEECCCCCCCHHHHCCCCEEEECHHHH
IQSYLAEGTIVIVAGFQGVSEDFEITTLGRGGSDTTAVALAAALKAKKCDIYTDVTGVYT
HHHHHHCCCEEEEECCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEECCCEEEE
TDPRVVKDAYKLDEISYDEMLELANLGAGVLHPRAVEFAKNHNVVLEVRSSMEQENGTIV
CCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHCCCCEEEEEHHHHHHCCCCEE
KGECNMEQQSIVKGIAFEDNITRVTIKGLEQGSLSTVFSTLAAAHINVDIIIQSITNEGT
EEECCCCHHHHHHCCEECCCCEEEEEECCCCCCHHHHHHHHHHHHCEEEEEEEEECCCCE
VHLSFSIHSNDLRETLKVLEQNQETLHYESVEHENHLAKVSIVGSGMVSNPGVAANMFTT
EEEEEEECCHHHHHHHHHHHCCCHHEEHHCCCCCCCEEEEEEEECCCCCCCCCHHHHHHH
LKEEDIHIKMVSTSEIKVSVVIDRLHLVTGVEALHQSFMAKIEPFVQIS
HCCCCEEEEEEECCCEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCEEEC
>Mature Secondary Structure
METIVQKFGGTSVGSVERIQHVANLIIEEYERGHSIVSVVSAMGKSTDELVALANAITEN
CCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHCC
PSKREMDMLLSTGEQVTISLLTIALQAKGYHAISLTGWQAGITTESVHSSARITDIHTDR
CCHHHHHHHHHCCCCEEEEEEEEEEECCCEEEEEEECCCCCCCHHHHCCCCEEEECHHHH
IQSYLAEGTIVIVAGFQGVSEDFEITTLGRGGSDTTAVALAAALKAKKCDIYTDVTGVYT
HHHHHHCCCEEEEECCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEECCCEEEE
TDPRVVKDAYKLDEISYDEMLELANLGAGVLHPRAVEFAKNHNVVLEVRSSMEQENGTIV
CCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHCCCCEEEEEHHHHHHCCCCEE
KGECNMEQQSIVKGIAFEDNITRVTIKGLEQGSLSTVFSTLAAAHINVDIIIQSITNEGT
EEECCCCHHHHHHCCEECCCCEEEEEECCCCCCHHHHHHHHHHHHCEEEEEEEEECCCCE
VHLSFSIHSNDLRETLKVLEQNQETLHYESVEHENHLAKVSIVGSGMVSNPGVAANMFTT
EEEEEEECCHHHHHHHHHHHCCCHHEEHHCCCCCCCEEEEEEEECCCCCCCCCHHHHHHH
LKEEDIHIKMVSTSEIKVSVVIDRLHLVTGVEALHQSFMAKIEPFVQIS
HCCCCEEEEEEECCCEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1444390 [H]