Click here to switch to the map view.

The map label for this gene is vanS [H]

Identifier: 218232219

GI number: 218232219

Start: 263631

End: 265040

Strand: Direct

Name: vanS [H]

Synonym: BCB4264_A0304

Alternate gene names: 218232219

Gene position: 263631-265040 (Clockwise)

Preceding gene: 218232740

Following gene: 218235504

Centisome position: 4.86

GC content: 34.96

Gene sequence:

>1410_bases
TTGATTACTACTTTTGCTGCTTGTGCGTTATTAGGAGTTTTGTCAGCGAAGGCAGCAGCGCCGTTTTTTGAGAATGCGAA
TCGTCAAGCGACTATTGATTATCGGGCTGGTATGGAACAAATTAATCACCAAGCTCAAGGAGCGGCAAATAGTTCGGTTC
ATGAAAATAAATTAGAAGCTATATCAAATATGATCGAAATGGAGAACCAAAATTTAGAGCAAGGGTCTAGAGCTTTGAAG
ATATTGGTTACTGATGAAAGTGGTAAAGTTTTATATAAAACAAAGCAAGCGCAAGAAGAGCAAATCGATTTGCATAATAC
GATTCGTAATGCAGCATCATTCGCGATTAATTATACAGATGGTAGAAATGTATTTGAAAGTACAAGAAAAGAGTTTATAT
CTTTTTCACCTATTACTATTGAAGGAAAGAATTTGTACATGTTTGTTAGCGGAATTCCAGAGGGAGAGGTAATATATGAC
ACGCAAGAAGGACCATTCCCGTTTTTAATTGGTGTACTTGTATTCATTTTCTCTTTCTTCTATATAACAAAGAGAAAGAT
GAAGCAGATTGAAGCGATGGCACAAGGCGTAAAGGAAATAGAAAGAGGTAACTTAGCCTACCGCATTGAGAAAAAAGGTG
AAGATGAGATTGCAGCTTTAACTGAGAATATTAATAATATGGCAGAAGAGCTTATGAATAATATAGAAAAGGAACGTAAA
TTAGAAAAGCAAAAGAATGAGCTTATCACAAATGTATCACATGATTTGCGTACACCACTTACTTCTATTATGGGTTACTT
ACGATTACTTCGAGATTCTAAATATGAAAATAAAGAACAACACGATGAGTATACGAGAATTGCTTTTGCAAAGTCGGAGC
AGTTAAAAAATTTAATAGAAGATTTATTTGAGTATACGAAGTTAACAAATGAACAAGTTGTATTAGAAAAACAAGAAGTA
TGTGTAAATGAGTTGCTTGAGCAATTAATAGAAGAGTTAGTACCGCAAGCGGAAGAGCATGGACTTACGTTTGTTAAGAA
GTTTCCTGAAGAACGTGCTTATGCATCGATTGATTCGGAAAAGATGGTTCGTGTATTTGATAATTTACTAATGAATGCAA
TTAAGTATAGTAAAGATGATGGAGAGATAAAAGTTTCTCTTCAAAGGCAGCGTAGGGATATACAAATTGTAATTGCGAAT
CATAGTGAAGAGTTTACAAGAGAAGAGTTAGCGAATTTGTTTGAACGTTTCTATAAGAAGGATCAATCTCGAAGTAGAGT
AACGGAAGGCTCGGGACTCGGTTTAGCGATTGCGAAAAGTATTGTTGAGTTACAAGGTGGTAGTATTCGAGCGGAATATA
GGGATGGCACTATTCAATTTATCGTCTCGTTACCAATTATAGAAAAATAA

Upstream 100 bases:

>100_bases
AAGATTGAAAAAGATATTTAATCCGTTTACTTATATAAGGAAAATAAGAGAATTATTTGAGAAATTAGTAAAGAGCGTAC
GAAAGAGTATAAGGATTCAA

Downstream 100 bases:

>100_bases
TAAATGAATAGAATGATATGTGATAAAAAGGCTTATTTTAAAAGAAATAAGCCTTTTTATATTTTTTATAAAAACATATT
GACGATTAGAGCTTAGAGGT

Product: sensor histidine kinase

Products: NA

Alternate protein names: Vancomycin histidine protein kinase; Vancomycin resistance protein vanS [H]

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MITTFAACALLGVLSAKAAAPFFENANRQATIDYRAGMEQINHQAQGAANSSVHENKLEAISNMIEMENQNLEQGSRALK
ILVTDESGKVLYKTKQAQEEQIDLHNTIRNAASFAINYTDGRNVFESTRKEFISFSPITIEGKNLYMFVSGIPEGEVIYD
TQEGPFPFLIGVLVFIFSFFYITKRKMKQIEAMAQGVKEIERGNLAYRIEKKGEDEIAALTENINNMAEELMNNIEKERK
LEKQKNELITNVSHDLRTPLTSIMGYLRLLRDSKYENKEQHDEYTRIAFAKSEQLKNLIEDLFEYTKLTNEQVVLEKQEV
CVNELLEQLIEELVPQAEEHGLTFVKKFPEERAYASIDSEKMVRVFDNLLMNAIKYSKDDGEIKVSLQRQRRDIQIVIAN
HSEEFTREELANLFERFYKKDQSRSRVTEGSGLGLAIAKSIVELQGGSIRAEYRDGTIQFIVSLPIIEK

Sequences:

>Translated_469_residues
MITTFAACALLGVLSAKAAAPFFENANRQATIDYRAGMEQINHQAQGAANSSVHENKLEAISNMIEMENQNLEQGSRALK
ILVTDESGKVLYKTKQAQEEQIDLHNTIRNAASFAINYTDGRNVFESTRKEFISFSPITIEGKNLYMFVSGIPEGEVIYD
TQEGPFPFLIGVLVFIFSFFYITKRKMKQIEAMAQGVKEIERGNLAYRIEKKGEDEIAALTENINNMAEELMNNIEKERK
LEKQKNELITNVSHDLRTPLTSIMGYLRLLRDSKYENKEQHDEYTRIAFAKSEQLKNLIEDLFEYTKLTNEQVVLEKQEV
CVNELLEQLIEELVPQAEEHGLTFVKKFPEERAYASIDSEKMVRVFDNLLMNAIKYSKDDGEIKVSLQRQRRDIQIVIAN
HSEEFTREELANLFERFYKKDQSRSRVTEGSGLGLAIAKSIVELQGGSIRAEYRDGTIQFIVSLPIIEK
>Mature_469_residues
MITTFAACALLGVLSAKAAAPFFENANRQATIDYRAGMEQINHQAQGAANSSVHENKLEAISNMIEMENQNLEQGSRALK
ILVTDESGKVLYKTKQAQEEQIDLHNTIRNAASFAINYTDGRNVFESTRKEFISFSPITIEGKNLYMFVSGIPEGEVIYD
TQEGPFPFLIGVLVFIFSFFYITKRKMKQIEAMAQGVKEIERGNLAYRIEKKGEDEIAALTENINNMAEELMNNIEKERK
LEKQKNELITNVSHDLRTPLTSIMGYLRLLRDSKYENKEQHDEYTRIAFAKSEQLKNLIEDLFEYTKLTNEQVVLEKQEV
CVNELLEQLIEELVPQAEEHGLTFVKKFPEERAYASIDSEKMVRVFDNLLMNAIKYSKDDGEIKVSLQRQRRDIQIVIAN
HSEEFTREELANLFERFYKKDQSRSRVTEGSGLGLAIAKSIVELQGGSIRAEYRDGTIQFIVSLPIIEK

Specific function: Member of the two-component regulatory system vanS/vanR. Activates the transcription of vanH, vanA and vanX in response to vancomycin which results in vancomycin resistance. VanS activates vanR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788393, Length=281, Percent_Identity=25.9786476868327, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1786783, Length=308, Percent_Identity=25.3246753246753, Blast_Score=102, Evalue=7e-23,
Organism=Escherichia coli, GI1788713, Length=249, Percent_Identity=28.1124497991968, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1790346, Length=284, Percent_Identity=25.7042253521127, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1787894, Length=341, Percent_Identity=28.1524926686217, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1789149, Length=338, Percent_Identity=26.0355029585799, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI145693157, Length=247, Percent_Identity=27.5303643724696, Blast_Score=88, Evalue=9e-19,
Organism=Escherichia coli, GI1786912, Length=255, Percent_Identity=26.6666666666667, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1786600, Length=209, Percent_Identity=26.7942583732057, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87082128, Length=308, Percent_Identity=27.9220779220779, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788549, Length=283, Percent_Identity=24.0282685512367, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI48994928, Length=220, Percent_Identity=25.4545454545455, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1788279, Length=254, Percent_Identity=26.3779527559055, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1790436, Length=227, Percent_Identity=26.431718061674, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1789808, Length=280, Percent_Identity=26.4285714285714, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 53662; Mature: 53662

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITTFAACALLGVLSAKAAAPFFENANRQATIDYRAGMEQINHQAQGAANSSVHENKLEA
CCCHHHHHHHHHHHHHHHCCCHHHCCCCEEEEHHHHHHHHHHHHHCCCCCCCHHHHHHHH
ISNMIEMENQNLEQGSRALKILVTDESGKVLYKTKQAQEEQIDLHNTIRNAASFAINYTD
HHHHHHHCCCCHHCCCCEEEEEEECCCCCEEEECHHHHHHHHHHHHHHHHHHHEEEECCC
GRNVFESTRKEFISFSPITIEGKNLYMFVSGIPEGEVIYDTQEGPFPFLIGVLVFIFSFF
CCHHHHHHHHHHHCCCCEEEECCEEEEEEECCCCCCEEEECCCCCHHHHHHHHHHHHHHH
YITKRKMKQIEAMAQGVKEIERGNLAYRIEKKGEDEIAALTENINNMAEELMNNIEKERK
HHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LEKQKNELITNVSHDLRTPLTSIMGYLRLLRDSKYENKEQHDEYTRIAFAKSEQLKNLIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
DLFEYTKLTNEQVVLEKQEVCVNELLEQLIEELVPQAEEHGLTFVKKFPEERAYASIDSE
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHCCHHHHHHCCCHH
KMVRVFDNLLMNAIKYSKDDGEIKVSLQRQRRDIQIVIANHSEEFTREELANLFERFYKK
HHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHHHHC
DQSRSRVTEGSGLGLAIAKSIVELQGGSIRAEYRDGTIQFIVSLPIIEK
CCHHHHCCCCCCCHHHHHHHHHHHCCCCEEEEECCCEEEEEEECCCCCC
>Mature Secondary Structure
MITTFAACALLGVLSAKAAAPFFENANRQATIDYRAGMEQINHQAQGAANSSVHENKLEA
CCCHHHHHHHHHHHHHHHCCCHHHCCCCEEEEHHHHHHHHHHHHHCCCCCCCHHHHHHHH
ISNMIEMENQNLEQGSRALKILVTDESGKVLYKTKQAQEEQIDLHNTIRNAASFAINYTD
HHHHHHHCCCCHHCCCCEEEEEEECCCCCEEEECHHHHHHHHHHHHHHHHHHHEEEECCC
GRNVFESTRKEFISFSPITIEGKNLYMFVSGIPEGEVIYDTQEGPFPFLIGVLVFIFSFF
CCHHHHHHHHHHHCCCCEEEECCEEEEEEECCCCCCEEEECCCCCHHHHHHHHHHHHHHH
YITKRKMKQIEAMAQGVKEIERGNLAYRIEKKGEDEIAALTENINNMAEELMNNIEKERK
HHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LEKQKNELITNVSHDLRTPLTSIMGYLRLLRDSKYENKEQHDEYTRIAFAKSEQLKNLIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
DLFEYTKLTNEQVVLEKQEVCVNELLEQLIEELVPQAEEHGLTFVKKFPEERAYASIDSE
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHCCHHHHHHCCCHH
KMVRVFDNLLMNAIKYSKDDGEIKVSLQRQRRDIQIVIANHSEEFTREELANLFERFYKK
HHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHHHHC
DQSRSRVTEGSGLGLAIAKSIVELQGGSIRAEYRDGTIQFIVSLPIIEK
CCHHHHCCCCCCCHHHHHHHHHHHCCCCEEEEECCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8380148; 1556077 [H]