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The map label for this gene is kinE [H]

Identifier: 218232217

GI number: 218232217

Start: 382377

End: 383603

Strand: Direct

Name: kinE [H]

Synonym: BCB4264_A0406

Alternate gene names: 218232217

Gene position: 382377-383603 (Clockwise)

Preceding gene: 218232833

Following gene: 218233084

Centisome position: 7.06

GC content: 31.21

Gene sequence:

>1227_bases
ATGAATAAATACAAGAGCTTGATTTATGAAGAAAGAAGAGCCTTAAAGGTATTTATATTATTATTTTATATTATATTCTT
TTTATATGACGCTATATATTATTTTGTATATCCTGCTATGAATATTAGTGGAACAGCGGTAGGGTGGCCAGAAGGTGGTC
TTGGGGTAGGTGTGCCTATATTTGTAATAGCATTATTTCCTATTTCTATATATCTTCAAAAAAGAGGGTATGTATATTCT
GTAAAATATTTATTTTTATTTGGGTACATGTTTATCAATCTTGTGAATAATTTAATGATTTATTTGCGAACGGATAGACC
TTTTGAACATGGCAGTATGGTAGAAATGTTATTAATTTTATTTGCCCCAGTTTTCGTGAATAAAAAATACTTTTATTTAG
TGTCCTTTAGTGTAATTGGAAAGTACGTATTTTTCACATTGCTATTACAAGATTTAAAAGTGATAATTCCACTTGTTTTA
TGTGTATTTTATTTCATTATTTCACATTCTTTATTAAAACGATTTCAATCTTATGTAAGAACAGTGGTTGAAATGATGAA
CAATATGAAAGAGACGGAAAATTTAGCTGTGATTGGGACTATGTCTACAACAATTGCCCATGAAATTCGAAATCCGTTAA
CAGCGTTAAAAGGGTTTACGCAAATGCAAAAAGAAAGAAACCCAACTGATACGATGAGCTACGACATTATGCTACAAGAA
ATTGAAAGAGTCAATGGATTTGTTAGTGAATTGATGTTGTTAGGAAAACCAAAGCCAACAAATTATGAAAGGTGTAATAT
ACGAGACATCCTTCTATATGTTGTGCATCTAATGGAAAGTTATGCAACGCAATATAGAGTTAAATGTCATTTACAAGTAG
ATGGGAATTTACCGGCTATAAACGGTGATGATAAGCAATTAAAACAAGTGTTATTAAATATCATTAAAAATGGAATAGAA
TCAATGCCAGAGGGTGGAGAAATTCATATCCGTGCATATCAAAAAACGCAAGGACATTTGTGTATTTCTGTTGAAGACCA
AGGATTTGGTATAGAGAATGAGAAGTTAGAAAAAATCGGCAAGGCGTTTTACACAACAAAGGAGACTGGTACAGGACTTG
GATTAATGATTACATATAAAATTATTAAGGAACATCAAGGGAGTATCACGATCCAAAGTAGTGTAGGAGTAGGAACGAAA
GTAGAAATTTTTTTGCCTACCTTTTAA

Upstream 100 bases:

>100_bases
AGTATTATAAGGTTAAATATTGCCCTGAAAGCAATTTCATACTTATACTTTAAGGAGATAACAATGAAAGTTTTTTGTAT
CGATCAAGGGGAGTTTTTAT

Downstream 100 bases:

>100_bases
TGTATTGGGAGAAAAGATTTTTCAGAAAAGTGTTCTTGTTGTGTAAATATAAACATTTTATAAAATGTTTCAAGAAGGGT
TTTTTTTATAAAATACTTCG

Product: sporulation kinase

Products: NA

Alternate protein names: Sensor histidine kinase E [H]

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MNKYKSLIYEERRALKVFILLFYIIFFLYDAIYYFVYPAMNISGTAVGWPEGGLGVGVPIFVIALFPISIYLQKRGYVYS
VKYLFLFGYMFINLVNNLMIYLRTDRPFEHGSMVEMLLILFAPVFVNKKYFYLVSFSVIGKYVFFTLLLQDLKVIIPLVL
CVFYFIISHSLLKRFQSYVRTVVEMMNNMKETENLAVIGTMSTTIAHEIRNPLTALKGFTQMQKERNPTDTMSYDIMLQE
IERVNGFVSELMLLGKPKPTNYERCNIRDILLYVVHLMESYATQYRVKCHLQVDGNLPAINGDDKQLKQVLLNIIKNGIE
SMPEGGEIHIRAYQKTQGHLCISVEDQGFGIENEKLEKIGKAFYTTKETGTGLGLMITYKIIKEHQGSITIQSSVGVGTK
VEIFLPTF

Sequences:

>Translated_408_residues
MNKYKSLIYEERRALKVFILLFYIIFFLYDAIYYFVYPAMNISGTAVGWPEGGLGVGVPIFVIALFPISIYLQKRGYVYS
VKYLFLFGYMFINLVNNLMIYLRTDRPFEHGSMVEMLLILFAPVFVNKKYFYLVSFSVIGKYVFFTLLLQDLKVIIPLVL
CVFYFIISHSLLKRFQSYVRTVVEMMNNMKETENLAVIGTMSTTIAHEIRNPLTALKGFTQMQKERNPTDTMSYDIMLQE
IERVNGFVSELMLLGKPKPTNYERCNIRDILLYVVHLMESYATQYRVKCHLQVDGNLPAINGDDKQLKQVLLNIIKNGIE
SMPEGGEIHIRAYQKTQGHLCISVEDQGFGIENEKLEKIGKAFYTTKETGTGLGLMITYKIIKEHQGSITIQSSVGVGTK
VEIFLPTF
>Mature_408_residues
MNKYKSLIYEERRALKVFILLFYIIFFLYDAIYYFVYPAMNISGTAVGWPEGGLGVGVPIFVIALFPISIYLQKRGYVYS
VKYLFLFGYMFINLVNNLMIYLRTDRPFEHGSMVEMLLILFAPVFVNKKYFYLVSFSVIGKYVFFTLLLQDLKVIIPLVL
CVFYFIISHSLLKRFQSYVRTVVEMMNNMKETENLAVIGTMSTTIAHEIRNPLTALKGFTQMQKERNPTDTMSYDIMLQE
IERVNGFVSELMLLGKPKPTNYERCNIRDILLYVVHLMESYATQYRVKCHLQVDGNLPAINGDDKQLKQVLLNIIKNGIE
SMPEGGEIHIRAYQKTQGHLCISVEDQGFGIENEKLEKIGKAFYTTKETGTGLGLMITYKIIKEHQGSITIQSSVGVGTK
VEIFLPTF

Specific function: Phosphorylates the sporulation-regulatory protein spo0A under biofilm growth conditions. Also able to weakly phosphorylate spo0F [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=225, Percent_Identity=34.2222222222222, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI1790436, Length=256, Percent_Identity=31.25, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1786600, Length=217, Percent_Identity=28.5714285714286, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI1790300, Length=214, Percent_Identity=33.1775700934579, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1788713, Length=247, Percent_Identity=25.1012145748988, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1786783, Length=220, Percent_Identity=28.1818181818182, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1789149, Length=251, Percent_Identity=27.0916334661355, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790346, Length=215, Percent_Identity=24.1860465116279, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790551, Length=237, Percent_Identity=24.0506329113924, Blast_Score=62, Evalue=7e-11,
Organism=Escherichia coli, GI87081816, Length=217, Percent_Identity=25.8064516129032, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 46938; Mature: 46938

Theoretical pI: Translated: 8.99; Mature: 8.99

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKYKSLIYEERRALKVFILLFYIIFFLYDAIYYFVYPAMNISGTAVGWPEGGLGVGVPI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHH
FVIALFPISIYLQKRGYVYSVKYLFLFGYMFINLVNNLMIYLRTDRPFEHGSMVEMLLIL
HHHHHHHHHHEEECCCCEEHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCHHHHHHHH
FAPVFVNKKYFYLVSFSVIGKYVFFTLLLQDLKVIIPLVLCVFYFIISHSLLKRFQSYVR
HHHHHHCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TVVEMMNNMKETENLAVIGTMSTTIAHEIRNPLTALKGFTQMQKERNPTDTMSYDIMLQE
HHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
IERVNGFVSELMLLGKPKPTNYERCNIRDILLYVVHLMESYATQYRVKCHLQVDGNLPAI
HHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCEEEEEEEEECCCCCCC
NGDDKQLKQVLLNIIKNGIESMPEGGEIHIRAYQKTQGHLCISVEDQGFGIENEKLEKIG
CCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCEEEEEECCCCCCCHHHHHHHH
KAFYTTKETGTGLGLMITYKIIKEHQGSITIQSSVGVGTKVEIFLPTF
HHHHCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCEEEEEECCC
>Mature Secondary Structure
MNKYKSLIYEERRALKVFILLFYIIFFLYDAIYYFVYPAMNISGTAVGWPEGGLGVGVPI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHH
FVIALFPISIYLQKRGYVYSVKYLFLFGYMFINLVNNLMIYLRTDRPFEHGSMVEMLLIL
HHHHHHHHHHEEECCCCEEHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCHHHHHHHH
FAPVFVNKKYFYLVSFSVIGKYVFFTLLLQDLKVIIPLVLCVFYFIISHSLLKRFQSYVR
HHHHHHCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TVVEMMNNMKETENLAVIGTMSTTIAHEIRNPLTALKGFTQMQKERNPTDTMSYDIMLQE
HHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
IERVNGFVSELMLLGKPKPTNYERCNIRDILLYVVHLMESYATQYRVKCHLQVDGNLPAI
HHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCEEEEEEEEECCCCCCC
NGDDKQLKQVLLNIIKNGIESMPEGGEIHIRAYQKTQGHLCISVEDQGFGIENEKLEKIG
CCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCEEEEEECCCCCCCHHHHHHHH
KAFYTTKETGTGLGLMITYKIIKEHQGSITIQSSVGVGTKVEIFLPTF
HHHHCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]