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The map label for this gene is cadA [H]

Identifier: 218232206

GI number: 218232206

Start: 617965

End: 620331

Strand: Direct

Name: cadA [H]

Synonym: BCB4264_A0634

Alternate gene names: 218232206

Gene position: 617965-620331 (Clockwise)

Preceding gene: 218234196

Following gene: 218233411

Centisome position: 11.4

GC content: 36.29

Gene sequence:

>2367_bases
ATGGCTGAAGCGTTAGTGAAAAAGAAACTGATGTTAGAAGGTTTAGATTGTGCGAATTGTGCAATGAAAATTGAAAAAGG
TGTTGGGAATATAGAAGGAGTAAATTCTTGCTCTGTAAACTTTGCAACAAAGACGATGATTTTAGAAACGGCACAAAATA
AAGAAAACGAAGTTGTTACGGAAGCAAAACAACTCGTTACAAAATTAGAACCGCATATTAAAGTGCAAGAAGAACAAAAA
AATAAAATTGCTAAAGAAGTATTTATATTAGAAGGTTTAGATTGCGCGAACTGTGCAATGAAAATTGAAAATAAAGTGAA
GGAAATGCCAGCTGTCTCAGAAGCAACTGTTGATTTCGTATCGAAGAAACTACGAGTAGAAGTTGCGAATAAAAGAGAAC
TAGAAGCGACTGTAGCAAATATAACAAATGTCGTTCAAAAGTTAGAGCCAGACGTGAAAGTTGTTCGTGAAGAGAAGAAC
GATCATGACCACGGGCATAGTCATGATCATGGTGAAGCAAATGTGAAAAAGATGGTAGGGAGATTAGTGGTCGGCGGAAT
TTTGACAGCAATTGCTGCATTAGCGGGCTTACCACAAATGGTAACAATTCCGTTATTCGTCCTTGCTTATTTATTAATAG
GTGGAGATATCGTTTGGAGAGCGATAAGAAACATAACTCGTGGCCAAGTATTTGATGAAAACTTCTTAATGGCAATTGCA
ACTGTAGGAGCTTTTGCAATTCAACAATACTCAGAAGCTGTAGCAGTAATGCTATTTTATCAAGTAGGAGAACTATTCCA
AAGCATTGCGGTAAACCGCTCTCGAAAATCAATTACTTCATTAATGGATATTCGTCCTGATTATGCGAATGTAAAGGTTG
GAAATGAAACGAAACAAGTATCACCAGAAGATGTACAAATTGGCGATTATATTATCGTTAAGCCAGGTGAGAAAGTGCCG
TTAGACGGAAAAGTAATTGAAGGAACATCAATGGTAGATACTTCAGCATTAACAGGTGAATCTGTACCACGTGAAGTTGA
AGTTGGAAATGATGTATTAAGTGGCTTTGTGAACCAAAACGGTGTGTTGACAATTGAAGTTACAAAAGAATTCGGTGAAT
CAACTGTATCGAAAATTTTAGATTTAGTTCAAAATGCAAGCAGTAAAAAAGCACCAACGGAAAACTTTATTACGAAGTTT
GCACGTTACTACACTCCAGTTGTAGTTATTACAGCGGCAATCATGGCGTTTATTCCACCACTTATTTTAGAAGGAGCTAC
ATTCTCTGAGTGGATTTATAGAGCTTTAGTGTTCTTAGTAATCTCTTGTCCATGTGCGTTAGTTGTATCCATTCCTCTTG
GATTCTTTGGAGGTATTGGTGGTGCATCTAAAAGTGGTGTGTTAGTAAAAGGTAGTAACTATTTAGAAGCTTTAAATGAT
GTGAAATATATTGTTTTTGACAAAACAGGAACATTAACAAAAGGTGTTTTCAAAGTTACAAAAATGGAACCGAGCGAAGG
TACTACAAGTGAAGAGTTATTAGAGTATGCGGCATTTGCTGAAGTATATTCTAACCATCCAATTGCCCAATCTATTCGAA
AGGCATATGGAAAATCAATTGATGAAAAAATAATCGATGATTATAACGAAATTTCTGGTCACGGTACAGTTGTAAAAGTA
CAAGGAAAAGAAATTTTTGCAGGTAATGCAAAATTAATGAGAAAAGAAAATATTGAATTTAAGCAACCAGAAACAGTAGG
TACATTAGTTCATGTTGCTGTAGATGGAAGATATGCAGGTTATATTGTTATCTCTGATGAGGTAAAAGAGGATTCGAAAC
AAGCGATTCAAAAATTAAAAGAACTAGGTATTAAAAAGACAGTAATGTTAACTGGTGATGCAAAACCAGTTGGTGAAGCT
GTCGGTAAAGAATTAGGCTTAGATGAAGTTCATGCGGAATTACTACCGCAACAAAAAGTAGAAGAGATTGAAAAAATTGA
TGCAGCGAAGCACGGAAAAGAAAAAATTGCCTTCGTTGGTGACGGTATTAACGATACACCAGTATTAGCCCGTGCAGACG
TTGGTATTGCGATGGGTGGTTTAGGGTCAGATGCAGCAATTGAAGCGGCAGACATCGTTATTATGACTGATGAGCCTTCA
AAAATTGCGACAGCTGTAAAAATTGCAAAACGTACAAGAAGTATAGTGTGGCAAAATATCATCTTTGCTTTAGGTGTAAA
AGGGATCGTTTTATTACTTGGTGCTTTTGGTATTGCAACAATGTGGGAAGCTGTTTTCTCAGATGTTGGTGTGACACTAC
TTGCAGTGTTAAATGCAATGCGTGTATTAAGAGTGAAAGATTTATAA

Upstream 100 bases:

>100_bases
TTTATTATTCGTTAGCTGACCAGCACGTAATTCATATCTTCGAGCAAGCGTTTGAACACGTAAACGAAGAAGAATAAAAA
GAACGCGAGGAGGGAGAACG

Downstream 100 bases:

>100_bases
AAAAGAGGACGCGACCCGCGTCCTCTTTTTTATTGGTGATTTTGTTGAAATTGACTCAATAAAATAATACCAAATATTAT
TTTATGTTTAAATAGAGTTA

Product: heavy metal-transporting ATPase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 788; Mature: 787

Protein sequence:

>788_residues
MAEALVKKKLMLEGLDCANCAMKIEKGVGNIEGVNSCSVNFATKTMILETAQNKENEVVTEAKQLVTKLEPHIKVQEEQK
NKIAKEVFILEGLDCANCAMKIENKVKEMPAVSEATVDFVSKKLRVEVANKRELEATVANITNVVQKLEPDVKVVREEKN
DHDHGHSHDHGEANVKKMVGRLVVGGILTAIAALAGLPQMVTIPLFVLAYLLIGGDIVWRAIRNITRGQVFDENFLMAIA
TVGAFAIQQYSEAVAVMLFYQVGELFQSIAVNRSRKSITSLMDIRPDYANVKVGNETKQVSPEDVQIGDYIIVKPGEKVP
LDGKVIEGTSMVDTSALTGESVPREVEVGNDVLSGFVNQNGVLTIEVTKEFGESTVSKILDLVQNASSKKAPTENFITKF
ARYYTPVVVITAAIMAFIPPLILEGATFSEWIYRALVFLVISCPCALVVSIPLGFFGGIGGASKSGVLVKGSNYLEALND
VKYIVFDKTGTLTKGVFKVTKMEPSEGTTSEELLEYAAFAEVYSNHPIAQSIRKAYGKSIDEKIIDDYNEISGHGTVVKV
QGKEIFAGNAKLMRKENIEFKQPETVGTLVHVAVDGRYAGYIVISDEVKEDSKQAIQKLKELGIKKTVMLTGDAKPVGEA
VGKELGLDEVHAELLPQQKVEEIEKIDAAKHGKEKIAFVGDGINDTPVLARADVGIAMGGLGSDAAIEAADIVIMTDEPS
KIATAVKIAKRTRSIVWQNIIFALGVKGIVLLLGAFGIATMWEAVFSDVGVTLLAVLNAMRVLRVKDL

Sequences:

>Translated_788_residues
MAEALVKKKLMLEGLDCANCAMKIEKGVGNIEGVNSCSVNFATKTMILETAQNKENEVVTEAKQLVTKLEPHIKVQEEQK
NKIAKEVFILEGLDCANCAMKIENKVKEMPAVSEATVDFVSKKLRVEVANKRELEATVANITNVVQKLEPDVKVVREEKN
DHDHGHSHDHGEANVKKMVGRLVVGGILTAIAALAGLPQMVTIPLFVLAYLLIGGDIVWRAIRNITRGQVFDENFLMAIA
TVGAFAIQQYSEAVAVMLFYQVGELFQSIAVNRSRKSITSLMDIRPDYANVKVGNETKQVSPEDVQIGDYIIVKPGEKVP
LDGKVIEGTSMVDTSALTGESVPREVEVGNDVLSGFVNQNGVLTIEVTKEFGESTVSKILDLVQNASSKKAPTENFITKF
ARYYTPVVVITAAIMAFIPPLILEGATFSEWIYRALVFLVISCPCALVVSIPLGFFGGIGGASKSGVLVKGSNYLEALND
VKYIVFDKTGTLTKGVFKVTKMEPSEGTTSEELLEYAAFAEVYSNHPIAQSIRKAYGKSIDEKIIDDYNEISGHGTVVKV
QGKEIFAGNAKLMRKENIEFKQPETVGTLVHVAVDGRYAGYIVISDEVKEDSKQAIQKLKELGIKKTVMLTGDAKPVGEA
VGKELGLDEVHAELLPQQKVEEIEKIDAAKHGKEKIAFVGDGINDTPVLARADVGIAMGGLGSDAAIEAADIVIMTDEPS
KIATAVKIAKRTRSIVWQNIIFALGVKGIVLLLGAFGIATMWEAVFSDVGVTLLAVLNAMRVLRVKDL
>Mature_787_residues
AEALVKKKLMLEGLDCANCAMKIEKGVGNIEGVNSCSVNFATKTMILETAQNKENEVVTEAKQLVTKLEPHIKVQEEQKN
KIAKEVFILEGLDCANCAMKIENKVKEMPAVSEATVDFVSKKLRVEVANKRELEATVANITNVVQKLEPDVKVVREEKND
HDHGHSHDHGEANVKKMVGRLVVGGILTAIAALAGLPQMVTIPLFVLAYLLIGGDIVWRAIRNITRGQVFDENFLMAIAT
VGAFAIQQYSEAVAVMLFYQVGELFQSIAVNRSRKSITSLMDIRPDYANVKVGNETKQVSPEDVQIGDYIIVKPGEKVPL
DGKVIEGTSMVDTSALTGESVPREVEVGNDVLSGFVNQNGVLTIEVTKEFGESTVSKILDLVQNASSKKAPTENFITKFA
RYYTPVVVITAAIMAFIPPLILEGATFSEWIYRALVFLVISCPCALVVSIPLGFFGGIGGASKSGVLVKGSNYLEALNDV
KYIVFDKTGTLTKGVFKVTKMEPSEGTTSEELLEYAAFAEVYSNHPIAQSIRKAYGKSIDEKIIDDYNEISGHGTVVKVQ
GKEIFAGNAKLMRKENIEFKQPETVGTLVHVAVDGRYAGYIVISDEVKEDSKQAIQKLKELGIKKTVMLTGDAKPVGEAV
GKELGLDEVHAELLPQQKVEEIEKIDAAKHGKEKIAFVGDGINDTPVLARADVGIAMGGLGSDAAIEAADIVIMTDEPSK
IATAVKIAKRTRSIVWQNIIFALGVKGIVLLLGAFGIATMWEAVFSDVGVTLLAVLNAMRVLRVKDL

Specific function: Couples the hydrolysis of ATP with the transport of cadmium, zinc and cobalt out of the cell. Does not seem to transport copper [H]

COG id: COG2217

COG function: function code P; Cation transport ATPase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HMA domain [H]

Homologues:

Organism=Homo sapiens, GI55743071, Length=867, Percent_Identity=25.3748558246828, Blast_Score=259, Evalue=8e-69,
Organism=Homo sapiens, GI55743073, Length=543, Percent_Identity=28.3609576427256, Blast_Score=218, Evalue=2e-56,
Organism=Homo sapiens, GI115529486, Length=653, Percent_Identity=24.8085758039816, Blast_Score=170, Evalue=4e-42,
Organism=Homo sapiens, GI118498343, Length=212, Percent_Identity=28.3018867924528, Blast_Score=70, Evalue=8e-12,
Organism=Homo sapiens, GI22748667, Length=392, Percent_Identity=26.0204081632653, Blast_Score=68, Evalue=4e-11,
Organism=Escherichia coli, GI1789879, Length=705, Percent_Identity=34.3262411347518, Blast_Score=358, Evalue=1e-100,
Organism=Escherichia coli, GI1786691, Length=853, Percent_Identity=27.3153575615475, Blast_Score=274, Evalue=1e-74,
Organism=Escherichia coli, GI1786914, Length=524, Percent_Identity=28.4351145038168, Blast_Score=155, Evalue=8e-39,
Organism=Escherichia coli, GI2367363, Length=592, Percent_Identity=24.3243243243243, Blast_Score=84, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17556548, Length=905, Percent_Identity=26.6298342541436, Blast_Score=218, Evalue=1e-56,
Organism=Saccharomyces cerevisiae, GI6320475, Length=560, Percent_Identity=30.5357142857143, Blast_Score=226, Evalue=8e-60,
Organism=Saccharomyces cerevisiae, GI6319772, Length=635, Percent_Identity=24.8818897637795, Blast_Score=172, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6325221, Length=562, Percent_Identity=22.4199288256228, Blast_Score=88, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6321430, Length=568, Percent_Identity=22.1830985915493, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6321271, Length=220, Percent_Identity=26.3636363636364, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI221329854, Length=684, Percent_Identity=26.6081871345029, Blast_Score=166, Evalue=4e-41,
Organism=Drosophila melanogaster, GI24668704, Length=157, Percent_Identity=26.7515923566879, Blast_Score=72, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24668708, Length=157, Percent_Identity=26.7515923566879, Blast_Score=72, Evalue=2e-12,
Organism=Drosophila melanogaster, GI281366617, Length=157, Percent_Identity=26.7515923566879, Blast_Score=72, Evalue=2e-12,
Organism=Drosophila melanogaster, GI161085803, Length=157, Percent_Identity=26.7515923566879, Blast_Score=71, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24668696, Length=157, Percent_Identity=26.7515923566879, Blast_Score=71, Evalue=3e-12,
Organism=Drosophila melanogaster, GI281366676, Length=267, Percent_Identity=26.2172284644195, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008250
- InterPro:   IPR001366
- InterPro:   IPR006404
- InterPro:   IPR006416
- InterPro:   IPR001757
- InterPro:   IPR018303
- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR017969
- InterPro:   IPR006121 [H]

Pfam domain/function: PF00122 E1-E2_ATPase; PF00403 HMA; PF00702 Hydrolase [H]

EC number: =3.6.3.3; =3.6.3.5 [H]

Molecular weight: Translated: 85439; Mature: 85308

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS00154 ATPASE_E1_E2 ; PS01047 HMA_1 ; PS50846 HMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEALVKKKLMLEGLDCANCAMKIEKGVGNIEGVNSCSVNFATKTMILETAQNKENEVVT
CCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHCCCCHHHHH
EAKQLVTKLEPHIKVQEEQKNKIAKEVFILEGLDCANCAMKIENKVKEMPAVSEATVDFV
HHHHHHHHCCCCCEECHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHH
SKKLRVEVANKRELEATVANITNVVQKLEPDVKVVREEKNDHDHGHSHDHGEANVKKMVG
HHHHHEEECCCHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHH
RLVVGGILTAIAALAGLPQMVTIPLFVLAYLLIGGDIVWRAIRNITRGQVFDENFLMAIA
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
TVGAFAIQQYSEAVAVMLFYQVGELFQSIAVNRSRKSITSLMDIRPDYANVKVGNETKQV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEECCCCCCC
SPEDVQIGDYIIVKPGEKVPLDGKVIEGTSMVDTSALTGESVPREVEVGNDVLSGFVNQN
CCCCEEECCEEEECCCCCCCCCCEEECCCCCCCCHHCCCCCCCCHHHCCHHHHHHHCCCC
GVLTIEVTKEFGESTVSKILDLVQNASSKKAPTENFITKFARYYTPVVVITAAIMAFIPP
CEEEEEEHHHHCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LILEGATFSEWIYRALVFLVISCPCALVVSIPLGFFGGIGGASKSGVLVKGSNYLEALND
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCHHHHHHCC
VKYIVFDKTGTLTKGVFKVTKMEPSEGTTSEELLEYAAFAEVYSNHPIAQSIRKAYGKSI
CEEEEEECCCCHHHHHEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCH
DEKIIDDYNEISGHGTVVKVQGKEIFAGNAKLMRKENIEFKQPETVGTLVHVAVDGRYAG
HHHHHHHHHHCCCCCCEEEEECCEEEECCHHHHHHCCCCCCCCCHHCEEEEEEECCCEEE
YIVISDEVKEDSKQAIQKLKELGIKKTVMLTGDAKPVGEAVGKELGLDEVHAELLPQQKV
EEEEECHHCHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCHHHHHHHHCCHHHH
EEIEKIDAAKHGKEKIAFVGDGINDTPVLARADVGIAMGGLGSDAAIEAADIVIMTDEPS
HHHHHHHHHHCCCCEEEEEECCCCCCCEEEECCCCEEECCCCCCCCEEEEEEEEECCCCH
KIATAVKIAKRTRSIVWQNIIFALGVKGIVLLLGAFGIATMWEAVFSDVGVTLLAVLNAM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVLRVKDL
HHHHHCCC
>Mature Secondary Structure 
AEALVKKKLMLEGLDCANCAMKIEKGVGNIEGVNSCSVNFATKTMILETAQNKENEVVT
CHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHCCCCHHHHH
EAKQLVTKLEPHIKVQEEQKNKIAKEVFILEGLDCANCAMKIENKVKEMPAVSEATVDFV
HHHHHHHHCCCCCEECHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHH
SKKLRVEVANKRELEATVANITNVVQKLEPDVKVVREEKNDHDHGHSHDHGEANVKKMVG
HHHHHEEECCCHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHH
RLVVGGILTAIAALAGLPQMVTIPLFVLAYLLIGGDIVWRAIRNITRGQVFDENFLMAIA
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
TVGAFAIQQYSEAVAVMLFYQVGELFQSIAVNRSRKSITSLMDIRPDYANVKVGNETKQV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEECCCCCCC
SPEDVQIGDYIIVKPGEKVPLDGKVIEGTSMVDTSALTGESVPREVEVGNDVLSGFVNQN
CCCCEEECCEEEECCCCCCCCCCEEECCCCCCCCHHCCCCCCCCHHHCCHHHHHHHCCCC
GVLTIEVTKEFGESTVSKILDLVQNASSKKAPTENFITKFARYYTPVVVITAAIMAFIPP
CEEEEEEHHHHCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LILEGATFSEWIYRALVFLVISCPCALVVSIPLGFFGGIGGASKSGVLVKGSNYLEALND
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCHHHHHHCC
VKYIVFDKTGTLTKGVFKVTKMEPSEGTTSEELLEYAAFAEVYSNHPIAQSIRKAYGKSI
CEEEEEECCCCHHHHHEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCH
DEKIIDDYNEISGHGTVVKVQGKEIFAGNAKLMRKENIEFKQPETVGTLVHVAVDGRYAG
HHHHHHHHHHCCCCCCEEEEECCEEEECCHHHHHHCCCCCCCCCHHCEEEEEEECCCEEE
YIVISDEVKEDSKQAIQKLKELGIKKTVMLTGDAKPVGEAVGKELGLDEVHAELLPQQKV
EEEEECHHCHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCHHHHHHHHCCHHHH
EEIEKIDAAKHGKEKIAFVGDGINDTPVLARADVGIAMGGLGSDAAIEAADIVIMTDEPS
HHHHHHHHHHCCCCEEEEEECCCCCCCEEEECCCCEEECCCCCCCCEEEEEEEEECCCCH
KIATAVKIAKRTRSIVWQNIIFALGVKGIVLLLGAFGIATMWEAVFSDVGVTLLAVLNAM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVLRVKDL
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]