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The map label for this gene is cynR [H]

Identifier: 218232168

GI number: 218232168

Start: 2295469

End: 2296371

Strand: Reverse

Name: cynR [H]

Synonym: BCB4264_A2396

Alternate gene names: 218232168

Gene position: 2296371-2295469 (Counterclockwise)

Preceding gene: 218231859

Following gene: 218232677

Centisome position: 42.38

GC content: 32.12

Gene sequence:

>903_bases
GTGATTCCAATCGAATTACGACAACTTGAATACTTTTTAGCTGTTTCAAAAGAATTACATTTCACAAAAGCAGCTGAAAA
ATTAAATATTTCACAGCCTTCTCTAAGTCAACAAATACGTGCATTAGAGCATGAAGTAGGAATGCCACTTTTCGATCGAA
TTGGTAAAAAAATATCATTAACTGAAGCAGGGAAGATTTTATTATCACATAGTAAAACCATTTTTCATGAAGTCGAACAA
GCTCGTTCAGCTATTCAAGACCTAAACGGATTACAGCAAGGTTCCTTAACAATAGGGGCTTTGTTAACCGTCGTAAATTA
TTTACTACCACCAGCTATTTTAAATTTTAATAATTTATATCCTAATATAGAGCTTTCTGTATTAGGGCTTCGTACAGGAG
ATATTCGTGAAAAATTATTACAAAACGAACTAGATATTGGAATTACATTTCTCCCGGTGCAAGATAAAGAGATTATCTCC
ATCCCTCTATACAAAAGCGATCTTACATTGGTCGTGCCCACTGGTCATCGATTAGCTGAACGTAATCATGTATCTATTGC
TGAATTACAGAACTACCCTTTAATTCTTTTACCTAAAAATTTCTTTTTAACCGAACTAATTACATCTCATTGTCAAAAGT
TAAACTTTAAACCAAGGCCAATTTTAGAAATTAGTACGATGGAATCTTTAATCCAAATGGTTTCCAAAGGAATGGGAATT
ACAGTGTTACCCAAACCATATATAGACTTTTTACAAAATAAAAACATTCAAGCTATTAAAATAGAAAACCCTACTCCAAT
AATTGAAATAGGGCTAATTTATAGAAAAGATAAATATATGTGTGCTGCAACTCGAGAATTTATTGAGCAACTAAAAATCA
CAGTACGTTCTTTCCAAAACTAA

Upstream 100 bases:

>100_bases
CCTCTCTGTTATATAAAGATGATTATAAAACCTCTAAAACCATTGTTCAATTCAATTATTTATATAATAATGATAGGTAT
AACCTATTATTATACTTGAG

Downstream 100 bases:

>100_bases
ACAAAAGAGATTATCCTATGAAACCGTTTCACAACAATTTAGTAGAATAATCTCTTTTCAAAACAAAGTAAACATTATAT
TTAACTCCTATAAACTTTAA

Product: transcriptional regulator, LysR family

Products: NA

Alternate protein names: Cyn operon transcriptional activator [H]

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MIPIELRQLEYFLAVSKELHFTKAAEKLNISQPSLSQQIRALEHEVGMPLFDRIGKKISLTEAGKILLSHSKTIFHEVEQ
ARSAIQDLNGLQQGSLTIGALLTVVNYLLPPAILNFNNLYPNIELSVLGLRTGDIREKLLQNELDIGITFLPVQDKEIIS
IPLYKSDLTLVVPTGHRLAERNHVSIAELQNYPLILLPKNFFLTELITSHCQKLNFKPRPILEISTMESLIQMVSKGMGI
TVLPKPYIDFLQNKNIQAIKIENPTPIIEIGLIYRKDKYMCAATREFIEQLKITVRSFQN

Sequences:

>Translated_300_residues
MIPIELRQLEYFLAVSKELHFTKAAEKLNISQPSLSQQIRALEHEVGMPLFDRIGKKISLTEAGKILLSHSKTIFHEVEQ
ARSAIQDLNGLQQGSLTIGALLTVVNYLLPPAILNFNNLYPNIELSVLGLRTGDIREKLLQNELDIGITFLPVQDKEIIS
IPLYKSDLTLVVPTGHRLAERNHVSIAELQNYPLILLPKNFFLTELITSHCQKLNFKPRPILEISTMESLIQMVSKGMGI
TVLPKPYIDFLQNKNIQAIKIENPTPIIEIGLIYRKDKYMCAATREFIEQLKITVRSFQN
>Mature_300_residues
MIPIELRQLEYFLAVSKELHFTKAAEKLNISQPSLSQQIRALEHEVGMPLFDRIGKKISLTEAGKILLSHSKTIFHEVEQ
ARSAIQDLNGLQQGSLTIGALLTVVNYLLPPAILNFNNLYPNIELSVLGLRTGDIREKLLQNELDIGITFLPVQDKEIIS
IPLYKSDLTLVVPTGHRLAERNHVSIAELQNYPLILLPKNFFLTELITSHCQKLNFKPRPILEISTMESLIQMVSKGMGI
TVLPKPYIDFLQNKNIQAIKIENPTPIIEIGLIYRKDKYMCAATREFIEQLKITVRSFQN

Specific function: Positively regulates the cynTSX operon, and negatively regulates its own transcription. Binds specifically to the cynR- cynTSX intergenic region [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI157672245, Length=282, Percent_Identity=34.3971631205674, Blast_Score=182, Evalue=2e-47,
Organism=Escherichia coli, GI145693105, Length=287, Percent_Identity=30.3135888501742, Blast_Score=137, Evalue=7e-34,
Organism=Escherichia coli, GI1790399, Length=295, Percent_Identity=29.1525423728814, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1788887, Length=291, Percent_Identity=31.2714776632302, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1787879, Length=292, Percent_Identity=31.1643835616438, Blast_Score=128, Evalue=6e-31,
Organism=Escherichia coli, GI2367136, Length=252, Percent_Identity=29.7619047619048, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1788481, Length=293, Percent_Identity=30.3754266211604, Blast_Score=110, Evalue=8e-26,
Organism=Escherichia coli, GI1787530, Length=291, Percent_Identity=23.3676975945017, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787806, Length=274, Percent_Identity=24.4525547445255, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1788297, Length=244, Percent_Identity=28.6885245901639, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1788748, Length=297, Percent_Identity=24.5791245791246, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1789204, Length=256, Percent_Identity=26.953125, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1787601, Length=297, Percent_Identity=24.9158249158249, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1790208, Length=244, Percent_Identity=24.5901639344262, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1788296, Length=232, Percent_Identity=26.7241379310345, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI1790262, Length=257, Percent_Identity=26.0700389105058, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI87081904, Length=201, Percent_Identity=30.3482587064677, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI87082132, Length=242, Percent_Identity=23.9669421487603, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1789173, Length=145, Percent_Identity=31.7241379310345, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787589, Length=301, Percent_Identity=24.2524916943522, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1790783, Length=253, Percent_Identity=27.6679841897233, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786401, Length=292, Percent_Identity=22.6027397260274, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1787128, Length=152, Percent_Identity=27.6315789473684, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1789639, Length=246, Percent_Identity=24.390243902439, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788626, Length=244, Percent_Identity=27.4590163934426, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1788706, Length=147, Percent_Identity=30.6122448979592, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1786713, Length=278, Percent_Identity=25.8992805755396, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34048; Mature: 34048

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPIELRQLEYFLAVSKELHFTKAAEKLNISQPSLSQQIRALEHEVGMPLFDRIGKKISL
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHCCCEEEE
TEAGKILLSHSKTIFHEVEQARSAIQDLNGLQQGSLTIGALLTVVNYLLPPAILNFNNLY
HHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHHCCCCCCC
PNIELSVLGLRTGDIREKLLQNELDIGITFLPVQDKEIISIPLYKSDLTLVVPTGHRLAE
CCEEEEEEEECCCHHHHHHHHHCCCCCEEEEECCCCCEEEEEEEECCCEEEEECCHHHHH
RNHVSIAELQNYPLILLPKNFFLTELITSHCQKLNFKPRPILEISTMESLIQMVSKGMGI
CCCCCHHHHCCCCEEEECCHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHCCCCE
TVLPKPYIDFLQNKNIQAIKIENPTPIIEIGLIYRKDKYMCAATREFIEQLKITVRSFQN
EECCCHHHHHHCCCCEEEEEECCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIPIELRQLEYFLAVSKELHFTKAAEKLNISQPSLSQQIRALEHEVGMPLFDRIGKKISL
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHCCCEEEE
TEAGKILLSHSKTIFHEVEQARSAIQDLNGLQQGSLTIGALLTVVNYLLPPAILNFNNLY
HHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHHCCCCCCC
PNIELSVLGLRTGDIREKLLQNELDIGITFLPVQDKEIISIPLYKSDLTLVVPTGHRLAE
CCEEEEEEEECCCHHHHHHHHHCCCCCEEEEECCCCCEEEEEEEECCCEEEEECCHHHHH
RNHVSIAELQNYPLILLPKNFFLTELITSHCQKLNFKPRPILEISTMESLIQMVSKGMGI
CCCCCHHHHCCCCEEEECCHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHCCCCE
TVLPKPYIDFLQNKNIQAIKIENPTPIIEIGLIYRKDKYMCAATREFIEQLKITVRSFQN
EECCCHHHHHHCCCCEEEEEECCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]