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The map label for this gene is yhcR [H]

Identifier: 218232142

GI number: 218232142

Start: 3029395

End: 3030984

Strand: Reverse

Name: yhcR [H]

Synonym: BCB4264_A3149

Alternate gene names: 218232142

Gene position: 3030984-3029395 (Counterclockwise)

Preceding gene: 218233264

Following gene: 218234244

Centisome position: 55.93

GC content: 35.41

Gene sequence:

>1590_bases
ATGTTGAAAAAAATCATTCCAGTTGCTTTAGCATTAAGTACAGTTACTTTTACTAGTGTATTTGCTGCGCCTTCAATCCA
AGCAAGTACAGAACAAAACCGCTACATAGATGTACAAATGCTCGGTATTAACGATTTTCATGGGCAACTAGACACTGTAA
AAAAAATTAACAATAAAGATGCAGGGGGAATTGAGTACTTAGGGGCTTATTTACGTGATCGTGAAAAACAAAATCCGAAT
ACATTAAAGGTACATGCTGGCGATGTTGTTGGAGCAAGTACTCCTGTTTCAGCATTATTACAAGACGAACCTACGATTGA
ATTTCTAAATGATTTGAAATTCGATGTTGGAACGATAGGAAATCATGAATTTGATGAAGGTGTTGAAGAAATGAACCGCC
TTATTTATGGTGGATACCATGAGAAAACAGGAAATTTTAAAGGAGCAAAATTCCCATATGTTGCTGCAAATTTCTATAAT
AAATCCACCGGTCGTTTATTTTTACCACCATTCACTATAAAAAAGGTACAAGGAGTTCCTGTTGGCTTTATCGGAGTCGT
AACAACGGATGTTCCTAACCTTGTTATGCCTACTATGCTAAAGAATGTAGAAATTACAGATGAAGTTGAAGCTATTAACA
AATCCGTAAAGCAATTAAAAAAACTAGGCGTTAAATCTATCGTTGTTCTTGCACATAACGGAGGTATAACAGATGGGAAC
GGCGTAACAAATGGTGATATCGTCCGATTAGCAAATGAGACTGATCCAGAAGTCGATGTTATTTTTGGTGGCCATAGCCA
CACCTATGTAAATGGAACTGTTAATAATAAACTTGTCGTACAAGCAAATTCATATGGTATGGCTTTTGCTGATGTTGATG
TAAAGATTGATCGTAAAACACAAGATATTGTTGAGAAAAAAGCAGAAATTGTTACAACTTACCATGAAGGCATGGAGCCT
GATAAAAAAATCAAGAAGAAGATGGAGAAATATCAAGCAAAAATCGCACCTCTTGTTAATGAAATTGTAGGTAAGTCTAT
TGCTCCACTAGATCGTAAACTCAATACGGCTGGTGAATCTACTCTCGGAAATTTAGTTGCTGATGCTCAGCGTGCAACAA
TGCAATCCAAAATTGCACTTATGAATCCTGGTGGTATTCGTAATGACTTAGATGCTGGTGATATTACATGGGGAGAGATA
TATGGTATTCAACCATTCGGAAATCAATTAATAAAAGTAAATTTAACAGGTCAAGACATTCGTGATATTTTAAATCAACA
ATGGCAAAAAGACATAACAAGAATGCTTCAAATTTCAGGGATCCAATACACTTGGGATGCGAACAAGCCTAATGGAGAAA
AAGTAACAAGTATTCGCTTAACAAATGGAGAAGAAATTATTCCTTCTAAAACTTACAGCGTCGTTGCGAACGCATTTCTA
GCTTCAGGTGGAGATGGATTTGTATCCTTTAAAAACGGTAAAGATGCTGAAACAGGACCAACTGATTTTGAAGCATTAGT
AGATTATATAAAAAAATCAAAAGAACCAATTCAGTCTATTATTGATGGAAGAATTCAAAAGATAAATTAG

Upstream 100 bases:

>100_bases
TTAATTTTTATAATTGGTAGAATTTTTGTGGAAATAATTCTCTTAATATATTAAAATAACATCGTATTATGTCAGATTTT
AATTAGGGGAGAGAAATTTT

Downstream 100 bases:

>100_bases
TTTTTTATTAATCTCAAAAACAACTAGATTACCTGTGCGCTATACTCTTTAGTGTGCAGGTAATCTATTTTTTTGATTTG
AAATAGTCTAACTGTGGCCA

Product: putative 5'-nucleotidase

Products: ribonucleoside; phosphate

Alternate protein names: NA

Number of amino acids: Translated: 529; Mature: 529

Protein sequence:

>529_residues
MLKKIIPVALALSTVTFTSVFAAPSIQASTEQNRYIDVQMLGINDFHGQLDTVKKINNKDAGGIEYLGAYLRDREKQNPN
TLKVHAGDVVGASTPVSALLQDEPTIEFLNDLKFDVGTIGNHEFDEGVEEMNRLIYGGYHEKTGNFKGAKFPYVAANFYN
KSTGRLFLPPFTIKKVQGVPVGFIGVVTTDVPNLVMPTMLKNVEITDEVEAINKSVKQLKKLGVKSIVVLAHNGGITDGN
GVTNGDIVRLANETDPEVDVIFGGHSHTYVNGTVNNKLVVQANSYGMAFADVDVKIDRKTQDIVEKKAEIVTTYHEGMEP
DKKIKKKMEKYQAKIAPLVNEIVGKSIAPLDRKLNTAGESTLGNLVADAQRATMQSKIALMNPGGIRNDLDAGDITWGEI
YGIQPFGNQLIKVNLTGQDIRDILNQQWQKDITRMLQISGIQYTWDANKPNGEKVTSIRLTNGEEIIPSKTYSVVANAFL
ASGGDGFVSFKNGKDAETGPTDFEALVDYIKKSKEPIQSIIDGRIQKIN

Sequences:

>Translated_529_residues
MLKKIIPVALALSTVTFTSVFAAPSIQASTEQNRYIDVQMLGINDFHGQLDTVKKINNKDAGGIEYLGAYLRDREKQNPN
TLKVHAGDVVGASTPVSALLQDEPTIEFLNDLKFDVGTIGNHEFDEGVEEMNRLIYGGYHEKTGNFKGAKFPYVAANFYN
KSTGRLFLPPFTIKKVQGVPVGFIGVVTTDVPNLVMPTMLKNVEITDEVEAINKSVKQLKKLGVKSIVVLAHNGGITDGN
GVTNGDIVRLANETDPEVDVIFGGHSHTYVNGTVNNKLVVQANSYGMAFADVDVKIDRKTQDIVEKKAEIVTTYHEGMEP
DKKIKKKMEKYQAKIAPLVNEIVGKSIAPLDRKLNTAGESTLGNLVADAQRATMQSKIALMNPGGIRNDLDAGDITWGEI
YGIQPFGNQLIKVNLTGQDIRDILNQQWQKDITRMLQISGIQYTWDANKPNGEKVTSIRLTNGEEIIPSKTYSVVANAFL
ASGGDGFVSFKNGKDAETGPTDFEALVDYIKKSKEPIQSIIDGRIQKIN
>Mature_529_residues
MLKKIIPVALALSTVTFTSVFAAPSIQASTEQNRYIDVQMLGINDFHGQLDTVKKINNKDAGGIEYLGAYLRDREKQNPN
TLKVHAGDVVGASTPVSALLQDEPTIEFLNDLKFDVGTIGNHEFDEGVEEMNRLIYGGYHEKTGNFKGAKFPYVAANFYN
KSTGRLFLPPFTIKKVQGVPVGFIGVVTTDVPNLVMPTMLKNVEITDEVEAINKSVKQLKKLGVKSIVVLAHNGGITDGN
GVTNGDIVRLANETDPEVDVIFGGHSHTYVNGTVNNKLVVQANSYGMAFADVDVKIDRKTQDIVEKKAEIVTTYHEGMEP
DKKIKKKMEKYQAKIAPLVNEIVGKSIAPLDRKLNTAGESTLGNLVADAQRATMQSKIALMNPGGIRNDLDAGDITWGEI
YGIQPFGNQLIKVNLTGQDIRDILNQQWQKDITRMLQISGIQYTWDANKPNGEKVTSIRLTNGEEIIPSKTYSVVANAFL
ASGGDGFVSFKNGKDAETGPTDFEALVDYIKKSKEPIQSIIDGRIQKIN

Specific function: Sugar-nonspecific endonuclease that yields nucleotide 3'-monophosphate products. No 5'-nucleotidase activity was detected, using 5'-AMP as the substrate, in the presence of diverse divalent metals and with various pH values [H]

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TNase-like domain [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=537, Percent_Identity=26.0707635009311, Blast_Score=143, Evalue=5e-34,
Organism=Escherichia coli, GI1786687, Length=551, Percent_Identity=26.1343012704174, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1790658, Length=565, Percent_Identity=23.0088495575221, Blast_Score=106, Evalue=5e-24,
Organism=Drosophila melanogaster, GI19922446, Length=561, Percent_Identity=27.807486631016, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI24654424, Length=561, Percent_Identity=27.807486631016, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI221329836, Length=560, Percent_Identity=25.8928571428571, Blast_Score=149, Evalue=4e-36,
Organism=Drosophila melanogaster, GI24641187, Length=561, Percent_Identity=26.2032085561497, Blast_Score=148, Evalue=1e-35,
Organism=Drosophila melanogaster, GI19921980, Length=449, Percent_Identity=24.7216035634744, Blast_Score=119, Evalue=7e-27,
Organism=Drosophila melanogaster, GI24652512, Length=449, Percent_Identity=24.4988864142539, Blast_Score=119, Evalue=8e-27,
Organism=Drosophila melanogaster, GI161076508, Length=449, Percent_Identity=24.4988864142539, Blast_Score=118, Evalue=9e-27,
Organism=Drosophila melanogaster, GI19922444, Length=506, Percent_Identity=25.296442687747, Blast_Score=118, Evalue=1e-26,
Organism=Drosophila melanogaster, GI28573524, Length=542, Percent_Identity=24.5387453874539, Blast_Score=118, Evalue=1e-26,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019948
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR016027
- InterPro:   IPR004365
- InterPro:   IPR016071
- InterPro:   IPR006021 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00746 Gram_pos_anchor; PF00149 Metallophos; PF00565 SNase; PF01336 tRNA_anti [H]

EC number: 3.1.3.5

Molecular weight: Translated: 57933; Mature: 57933

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: PS00786 5_NUCLEOTIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKKIIPVALALSTVTFTSVFAAPSIQASTEQNRYIDVQMLGINDFHGQLDTVKKINNKD
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHCCCC
AGGIEYLGAYLRDREKQNPNTLKVHAGDVVGASTPVSALLQDEPTIEFLNDLKFDVGTIG
CCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHHCCCCHHHHHHHCCCCCCCCC
NHEFDEGVEEMNRLIYGGYHEKTGNFKGAKFPYVAANFYNKSTGRLFLPPFTIKKVQGVP
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEECCCHHHHCCCCC
VGFIGVVTTDVPNLVMPTMLKNVEITDEVEAINKSVKQLKKLGVKSIVVLAHNGGITDGN
EEEEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCC
GVTNGDIVRLANETDPEVDVIFGGHSHTYVNGTVNNKLVVQANSYGMAFADVDVKIDRKT
CCCCCCEEEECCCCCCCEEEEECCCCEEEEECCCCCEEEEEECCCCEEEEEEEEEECCCH
QDIVEKKAEIVTTYHEGMEPDKKIKKKMEKYQAKIAPLVNEIVGKSIAPLDRKLNTAGES
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCHH
TLGNLVADAQRATMQSKIALMNPGGIRNDLDAGDITWGEIYGIQPFGNQLIKVNLTGQDI
HHHHHHHHHHHHHHHHCEEEECCCCCCCCCCCCCCCHHHEEEECCCCCEEEEEEECCHHH
RDILNQQWQKDITRMLQISGIQYTWDANKPNGEKVTSIRLTNGEEIIPSKTYSVVANAFL
HHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHH
ASGGDGFVSFKNGKDAETGPTDFEALVDYIKKSKEPIQSIIDGRIQKIN
HCCCCCEEEECCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHCCHHCCCC
>Mature Secondary Structure
MLKKIIPVALALSTVTFTSVFAAPSIQASTEQNRYIDVQMLGINDFHGQLDTVKKINNKD
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHCCCC
AGGIEYLGAYLRDREKQNPNTLKVHAGDVVGASTPVSALLQDEPTIEFLNDLKFDVGTIG
CCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHHCCCCHHHHHHHCCCCCCCCC
NHEFDEGVEEMNRLIYGGYHEKTGNFKGAKFPYVAANFYNKSTGRLFLPPFTIKKVQGVP
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEECCCHHHHCCCCC
VGFIGVVTTDVPNLVMPTMLKNVEITDEVEAINKSVKQLKKLGVKSIVVLAHNGGITDGN
EEEEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCC
GVTNGDIVRLANETDPEVDVIFGGHSHTYVNGTVNNKLVVQANSYGMAFADVDVKIDRKT
CCCCCCEEEECCCCCCCEEEEECCCCEEEEECCCCCEEEEEECCCCEEEEEEEEEECCCH
QDIVEKKAEIVTTYHEGMEPDKKIKKKMEKYQAKIAPLVNEIVGKSIAPLDRKLNTAGES
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCHH
TLGNLVADAQRATMQSKIALMNPGGIRNDLDAGDITWGEIYGIQPFGNQLIKVNLTGQDI
HHHHHHHHHHHHHHHHCEEEECCCCCCCCCCCCCCCHHHEEEECCCCCEEEEEEECCHHH
RDILNQQWQKDITRMLQISGIQYTWDANKPNGEKVTSIRLTNGEEIIPSKTYSVVANAFL
HHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHH
ASGGDGFVSFKNGKDAETGPTDFEALVDYIKKSKEPIQSIIDGRIQKIN
HCCCCCEEEECCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 5'-ribonucleotide; H2O

Specific reaction: A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969498; 9384377 [H]