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The map label for this gene is kinB2 [H]

Identifier: 218232135

GI number: 218232135

Start: 2578546

End: 2579715

Strand: Reverse

Name: kinB2 [H]

Synonym: BCB4264_A2669

Alternate gene names: 218232135

Gene position: 2579715-2578546 (Counterclockwise)

Preceding gene: 218234303

Following gene: 218234088

Centisome position: 47.6

GC content: 30.0

Gene sequence:

>1170_bases
TTGAAAAAATACAAAACATTACTGATTACCCTTTGTTCTAGTATCCCGCTTATTTTATGTATGCGATACCCTATCTATAT
GGATGAAAGTTGTATTCATGATTTAAGACAAATTCCGGTCATAATAGGTACGCTTTATGGTGGATTCCCTGTTGGTATCA
TATTGTTTACAATTTTATTAATTACAAGATTTTTATTTTATGGCTTTAACATGCTAACAGTCGTTGTTTACGGAATAATG
TTCATCGTTACAGCACTCGCATCTTCAAAATTTAATACATATAATAGAAAACTTAAAGTAGCTTTTTCCATGTTTTTAAC
CTTTTTCCTAGCAATATTTACAACTTTAATTGTATTAACTTTATCAGATTTTGAAGTAAATAATTTGTATATTATTTATT
TCATGGTATTACCGACTACTTTAATGTTATTTATTGTTTATATAAATGAAGTTATAAAAGATGCTGTATTAATGCGCTCT
AAATTAATTAAAATGGAAAAAATGGAGATTGTTAGCCAACTCGCTGCAAGTATATCGCACGAAGTAAGGAATCCCTTAAC
GGTTGTAAAAGGATTTACACAGCTTTTAAAAACACAAAATTTAACTCCTGAATCAAGAGACGAATATATTAAACATATAC
TTGAAGAGTTAAATCGTGCCCAAGAAATTATCGATGATTACTTAACGTTTGCCAAACCTGCTCCAGAAAAATTAGATCAA
ATTTCAGTAAAACAAGAGTTAAATCGAGTTATTAATATGATATTGCCATTGTGTAATATGAATACGATACATATTACACA
GGATTTTTCTGAGGCAACCATTGTTGGTAATAAACAGCACTTTCAACAATGTTTTTTAAACTTAATAAAAAACAGTATTG
AAGCAATGCCTAATGGTGGTACCTTAAATATTTCCTCATCTATCAATAATAGTAAAGTTGAAATACGAATTGAAGATAGC
GGAGTTGGAATGTCACAGGAGCAAATACATCGATTCGGTGAACCGTATTTTAGTACAAAAACAAAAGGAACTGGTTTAGG
TACAATGGTTGCTGTTAAGATTATTGAGACGATGCATGGAAGTTTAAAAATCCGAAGTATCGTTAGTAAAGGAACGACTT
TAACGATTACGTTCCCGAAATATAGTAAGAATACGCATTTAGACAAATAA

Upstream 100 bases:

>100_bases
TATGGACTTTGTTTATATTAATCAAAACGTATTAAATAATCTTCTATACATTCTATGTAGTATATTTGTTTTTTATTTCA
TATATGATAGCAGGCATTAT

Downstream 100 bases:

>100_bases
AAAAGGAGCATAGTTGCTCCTTTTTTATTTGTTTCGATACATAAGTATCTTCAATGTTTTTTTAGTTTACATTTGTGAAG
ACTAATTAAAAATTAGTATC

Product: sporulation kinase B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MKKYKTLLITLCSSIPLILCMRYPIYMDESCIHDLRQIPVIIGTLYGGFPVGIILFTILLITRFLFYGFNMLTVVVYGIM
FIVTALASSKFNTYNRKLKVAFSMFLTFFLAIFTTLIVLTLSDFEVNNLYIIYFMVLPTTLMLFIVYINEVIKDAVLMRS
KLIKMEKMEIVSQLAASISHEVRNPLTVVKGFTQLLKTQNLTPESRDEYIKHILEELNRAQEIIDDYLTFAKPAPEKLDQ
ISVKQELNRVINMILPLCNMNTIHITQDFSEATIVGNKQHFQQCFLNLIKNSIEAMPNGGTLNISSSINNSKVEIRIEDS
GVGMSQEQIHRFGEPYFSTKTKGTGLGTMVAVKIIETMHGSLKIRSIVSKGTTLTITFPKYSKNTHLDK

Sequences:

>Translated_389_residues
MKKYKTLLITLCSSIPLILCMRYPIYMDESCIHDLRQIPVIIGTLYGGFPVGIILFTILLITRFLFYGFNMLTVVVYGIM
FIVTALASSKFNTYNRKLKVAFSMFLTFFLAIFTTLIVLTLSDFEVNNLYIIYFMVLPTTLMLFIVYINEVIKDAVLMRS
KLIKMEKMEIVSQLAASISHEVRNPLTVVKGFTQLLKTQNLTPESRDEYIKHILEELNRAQEIIDDYLTFAKPAPEKLDQ
ISVKQELNRVINMILPLCNMNTIHITQDFSEATIVGNKQHFQQCFLNLIKNSIEAMPNGGTLNISSSINNSKVEIRIEDS
GVGMSQEQIHRFGEPYFSTKTKGTGLGTMVAVKIIETMHGSLKIRSIVSKGTTLTITFPKYSKNTHLDK
>Mature_389_residues
MKKYKTLLITLCSSIPLILCMRYPIYMDESCIHDLRQIPVIIGTLYGGFPVGIILFTILLITRFLFYGFNMLTVVVYGIM
FIVTALASSKFNTYNRKLKVAFSMFLTFFLAIFTTLIVLTLSDFEVNNLYIIYFMVLPTTLMLFIVYINEVIKDAVLMRS
KLIKMEKMEIVSQLAASISHEVRNPLTVVKGFTQLLKTQNLTPESRDEYIKHILEELNRAQEIIDDYLTFAKPAPEKLDQ
ISVKQELNRVINMILPLCNMNTIHITQDFSEATIVGNKQHFQQCFLNLIKNSIEAMPNGGTLNISSSINNSKVEIRIEDS
GVGMSQEQIHRFGEPYFSTKTKGTGLGTMVAVKIIETMHGSLKIRSIVSKGTTLTITFPKYSKNTHLDK

Specific function: Phosphorylates the sporulation-regulatory proteins spo0A and spo0F. Spo0F is required for the kinB activity [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=277, Percent_Identity=26.3537906137184, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1788549, Length=228, Percent_Identity=28.9473684210526, Blast_Score=118, Evalue=8e-28,
Organism=Escherichia coli, GI1789149, Length=240, Percent_Identity=29.5833333333333, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1786600, Length=264, Percent_Identity=24.6212121212121, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI145693157, Length=263, Percent_Identity=27.7566539923954, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1788713, Length=225, Percent_Identity=28, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1788393, Length=256, Percent_Identity=23.046875, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1790861, Length=242, Percent_Identity=21.4876033057851, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1790300, Length=233, Percent_Identity=23.6051502145923, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1786783, Length=230, Percent_Identity=22.6086956521739, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 44253; Mature: 44253

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CHHHHHHHHCCCEEEEECCCCCCCCCCCC
>Mature Secondary Structure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CHHHHHHHHCCCEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8497199; 9274030; 9384377 [H]