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The map label for this gene is brnQ1 [H]

Identifier: 218232051

GI number: 218232051

Start: 713533

End: 714834

Strand: Direct

Name: brnQ1 [H]

Synonym: BCB4264_A0722

Alternate gene names: 218232051

Gene position: 713533-714834 (Clockwise)

Preceding gene: 218232463

Following gene: 218232821

Centisome position: 13.17

GC content: 34.1

Gene sequence:

>1302_bases
ATGAAATTGTTACAGAAAAAAGAAATTTTACTTATTAGCCTTATGTTATTCTCTATGTTCTTTGGAGCAGGGAATCTTAT
ATTTCCACCTTTCCTTGGGTATGAAGCAGGAGAACATGTTTGGATTTCATTAGTAGGGTTTATTATATCAGCAACAGGTC
TTCCTATATTAGGTGTTATCTCTATTGCAAAAGCAGGAAGTTTTCAAACGTTAGCGGGTAGGGTTCATTCTTCATTTGCA
ATTATTTTTCCATGTATCGTGTATGTATTTATTGGACCTGGGCTTGGTATACCACGTGCGGGAAGTTTAGCTTTTGAAAT
GGGGCCAGGTCAGTTATTCCCTGAAGCAGGAAACGGATTATTATTGTTTTACACAGTTATTTTCTTTAGTATTGTTTACT
GGTTAAGTTTATCCCCATCTAAATTAATGGGGTTGTTTGGAAAAGTCTTGACGCCGTTATTATTATGTATGATTGCACTC
ATTTTTATAAAGAGTATGTTTACAACGGTAGGAGATATGAAAGAACCAATAGGGAACTATGGACAAGCTCCTATGTTTCA
AGGGTTTTTAGATGGATATTTAACAATGGATGCGTTAGCTGCTTTAATCTTTGGAATTGTTATTGCAAATGCATTACGTG
CAAAGGGTATTGAAGATGATAAAGGTTTAGCGAAATATATGAGTATTGCTGGAATTGGGGCAGGACTATTATTATCTATT
ATTTATGTCATCCTTGGATATGTGGGGGCAATTAGTGGCTCATTAGGAACATTTGATAACGGTGCGAAAGTGTTAGCACA
AGTTATGATTACATTATTTGGACAGGGCGGAGTCGTTTTATTAGGTCTTATTTTTACTATCGCGTGTTTATGTGTTTCAA
TTGGACTTGTTACGTCTTGTAGTCAATTTTTTGCGAGTGCATTTCCGAAAGTATCTTATAAAGTGTGGGCATTTATAGTA
AGCGTTGTTAGTATGATCTTAGCTAATTTAGGATTAACGCAAATTTTAAAAGTATCAGTACCAATTCTTGGATTTATTTA
TCCAATTGCATTAACGCTTATTATTCTAGGGTTATTCCATAAGTATATTGGGAAGTATGCATACGTATATGCAGTAACTG
TTTGGATTGTAGCAATATTTAGTGCAATTGATATTTTAAATAAAAATGTAATGATGAATCAGTGGACGTCAGTATTAAAA
TACATTCCGTTTTATACAGAAGGTGTTGGGTGGATTGTTCCAGCTATTATAGGCATGTGTCTTGGTTTCATCGTTAGTAT
TGCTTTAAATAAGAATAAATGA

Upstream 100 bases:

>100_bases
AGACTATGGACTATGTTTTCTTTGTAAGGAAGCGGGTTATTGTACTTTTTTATATGTGAAATTGATTTCATAAAAGATGA
AATTAAAGAAGGAGAAAGAA

Downstream 100 bases:

>100_bases
GTAAAAAGGTGTTTTCCTATTTTGGGAAGACACCTTTCTTTTTTTGGAAAAATACTATTGAAAATGAATAGACTAAGAGT
ATGATAAAGATAATTGGTAA

Product: branched-chain amino acid transport system II carrier protein

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: Branched-chain amino acid uptake carrier BrnQ [H]

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MKLLQKKEILLISLMLFSMFFGAGNLIFPPFLGYEAGEHVWISLVGFIISATGLPILGVISIAKAGSFQTLAGRVHSSFA
IIFPCIVYVFIGPGLGIPRAGSLAFEMGPGQLFPEAGNGLLLFYTVIFFSIVYWLSLSPSKLMGLFGKVLTPLLLCMIAL
IFIKSMFTTVGDMKEPIGNYGQAPMFQGFLDGYLTMDALAALIFGIVIANALRAKGIEDDKGLAKYMSIAGIGAGLLLSI
IYVILGYVGAISGSLGTFDNGAKVLAQVMITLFGQGGVVLLGLIFTIACLCVSIGLVTSCSQFFASAFPKVSYKVWAFIV
SVVSMILANLGLTQILKVSVPILGFIYPIALTLIILGLFHKYIGKYAYVYAVTVWIVAIFSAIDILNKNVMMNQWTSVLK
YIPFYTEGVGWIVPAIIGMCLGFIVSIALNKNK

Sequences:

>Translated_433_residues
MKLLQKKEILLISLMLFSMFFGAGNLIFPPFLGYEAGEHVWISLVGFIISATGLPILGVISIAKAGSFQTLAGRVHSSFA
IIFPCIVYVFIGPGLGIPRAGSLAFEMGPGQLFPEAGNGLLLFYTVIFFSIVYWLSLSPSKLMGLFGKVLTPLLLCMIAL
IFIKSMFTTVGDMKEPIGNYGQAPMFQGFLDGYLTMDALAALIFGIVIANALRAKGIEDDKGLAKYMSIAGIGAGLLLSI
IYVILGYVGAISGSLGTFDNGAKVLAQVMITLFGQGGVVLLGLIFTIACLCVSIGLVTSCSQFFASAFPKVSYKVWAFIV
SVVSMILANLGLTQILKVSVPILGFIYPIALTLIILGLFHKYIGKYAYVYAVTVWIVAIFSAIDILNKNVMMNQWTSVLK
YIPFYTEGVGWIVPAIIGMCLGFIVSIALNKNK
>Mature_433_residues
MKLLQKKEILLISLMLFSMFFGAGNLIFPPFLGYEAGEHVWISLVGFIISATGLPILGVISIAKAGSFQTLAGRVHSSFA
IIFPCIVYVFIGPGLGIPRAGSLAFEMGPGQLFPEAGNGLLLFYTVIFFSIVYWLSLSPSKLMGLFGKVLTPLLLCMIAL
IFIKSMFTTVGDMKEPIGNYGQAPMFQGFLDGYLTMDALAALIFGIVIANALRAKGIEDDKGLAKYMSIAGIGAGLLLSI
IYVILGYVGAISGSLGTFDNGAKVLAQVMITLFGQGGVVLLGLIFTIACLCVSIGLVTSCSQFFASAFPKVSYKVWAFIV
SVVSMILANLGLTQILKVSVPILGFIYPIALTLIILGLFHKYIGKYAYVYAVTVWIVAIFSAIDILNKNVMMNQWTSVLK
YIPFYTEGVGWIVPAIIGMCLGFIVSIALNKNK

Specific function: Component of the transport system for branched-chain amino acids (leucine, isoleucine and valine) Which is coupled to a proton motive force [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=436, Percent_Identity=34.1743119266055, Blast_Score=259, Evalue=2e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 46553; Mature: 46553

Theoretical pI: Translated: 9.30; Mature: 9.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHCCCC
>Mature Secondary Structure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HHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9023181; 9308178; 9384377 [H]