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The map label for this gene is colA [H]

Identifier: 218231943

GI number: 218231943

Start: 567364

End: 570261

Strand: Direct

Name: colA [H]

Synonym: BCB4264_A0591

Alternate gene names: 218231943

Gene position: 567364-570261 (Clockwise)

Preceding gene: 218231498

Following gene: 218235548

Centisome position: 10.47

GC content: 34.23

Gene sequence:

>2898_bases
ATGAACAAGAAATCAAAGATCAATAAAGTGATGCTTAGCATTAGTACAATGGCTTTATCGTTAGGCGCACTTCAAACTCA
TGCAGCAGCGGAAGAAAAAGTACCGTATAACGTGTTAAAAACGAAACCGGTTGGAATTGAAAAGTCGGTAGATGAAGTTG
GACATATTTCAAAAGTTGATGAAACTTTATCATTTCAAGAACGTTTAAAAGTAGGAGATTTTTCACAGCGACCAGCATCT
ATTACGAAGAAAACTGCAGTAAAGCAGGTTAAAGAAAGCTATTCAATGGCTGATTTAAACAAAATGAATGACCAAGAATT
AGTTGAAACGTTAGGCAGTATTAAATGGCACCAAATTACAGACTTATTCCAGTTTAATGAAGATGCAAAGGCTTTTTATA
AAGATAAAGGAAAAATGCAAGTCATTATAGATGAATTAGCTCATAGAGGTAGTACATTTACGAAAGATGATTCAAGAGGA
ATTCAAACGTTTACTGAAGTGCTGCGTTCAGCTTTTTATCTTGCATTTTATAATAGTGAATTAAGCGACTTAAATGAAAG
AAGCTTCCAGGATAAATGTTTACCTGCTTTAAAAGCAATCGCAAAAAATCCAAACTTTAAGCTTGGTACAGTTGAACAAG
ATACAGTCGTATCTGCGTACGGTAAATTAATTAGTAATGCTTCAAGCGATGTTGAAACGGTTCAATATGCATCGAATATT
TTAAAGCAATACAATGATAATTATACTACTTATGTAAATGATCGAATGAAGGGACAAGCAATATACGATATTATGCAAGG
TATTGACTATGATATGCAGTCGTACTTAACTGAGGCTCGTAAAGAAGCGAATGAAACGATGTGGTATGGAAAAGTAGATG
GGTTTATTAATGAAATAAATCGTATTGCTCTTCTAAATGAAGTAACGCCAGAAAATAAATGGCTCGTTAATAATGGCATT
TATTTTGCTAGCCGTTTAGGGAAGTTTCATAGCAATCCAAATAAAGGATTAGAGGTTGTTACACAAGCAATGCATATGTA
CCCGCGCTTAAGTGAACCGTATTTTGTTGCGGTAGAACAAATTACAACAAATTATAATGGTAAAGATTATAGCGGGAATA
CAGTAGATTTAGAGAAAATACGTAAAGAAGGAAAAGAGCAATACCTACCAAAAACGTATACATTCGACGATGGATCAATT
GTGTTCAAAACAGGAGATAAAGTATCAGAAGAAAAAATTAAGAGACTATATTGGGCTGCGAAGGAAGTAAAGGCACAGTA
TCACCGTGTAATTGGAAATGACAAAGCGTTAGAGCCAGGAAATGCGGATGATGTATTAACGATCGTAATTTATAATAGTC
CAGATGAATATCAGTTAAATAGACAATTGTATGGATATGAAACAAACAACGGTGGAATTTATATTGAAGAGACAGGTACA
TTCTTTACATATGAGCGTACACCAGAGCAAAGTATTTATAGTTTAGAAGAGTTATTCCGTCATGAATTTACTCATTATCT
GCAAGGGAGATATGAAGTTCCTGGTTTATTTGGAAGAGGAGATATGTATCAAAATGAAAGGCTAACTTGGTTCCAAGAAG
GAAATGCAGAGTTTTTCGCAGGATCTACTCGTACGAATAACGTTGTACCAAGAAAGAGTATAATTAGCGGATTATCATCT
GATCCTGCAAGCCGTTATACAGCAGAGCGTACACTATTTGCTAAATACGGTTCTTGGGATTTCTATAATTACTCGTTCGC
ATTGCAGTCTTACTTATATACGCATCAGTTTGAAACATTTGATAAAATTCAAGATTTGATTCGTGCGAATGACGTGAAAA
ATTATGATGCATATCGTGAAAATCTAAGTAAAGATCCTAAGTTAAATAAAGAGTATCAAGAGTATATGCAGCAGTTAATT
GATAATCAAGATAAATATAATGTACCGGAAGTAGCAGATGATTATTTAGCTGAACATGCACCGAAATCGTTAACTGAAGT
GAAAAAAGAAATTAGTGATACGTTGCCTATGAAAGATACAAAAATGACAAAACATAATTCTCAATTCTTTAATACATTTA
CATTAGAAGGTACGTATACAGGTAGTGTCACAAAAGGTGAATCAGAAGATTGGAAAGCAATGAGTAAAAGAGTAAATGAA
TCTTTAGAACAATTGGCGCAAAAAGAATGGAGTGGCTACAAAACTGTTACAGCATACTTCGTCAATTATCGTGTGAATAG
CTCAAATGAATTTGAATATGATGTAGTCTTCCATGGAATCGCAAAAGATGATGGAGAAAATAAAGCTCCAACGGTTAATA
TAAATGGCCCTTATAGCGGTCTTGTAAAAGAGGGAATTCAATTTAAAAGTGATGGCTCAAACGATGAAGATGGAAAAATT
GTTTCTTATTTATGGGAATTTGGAGATGGAAGCACAAGTGTAGAAGTGAATCCAGTACATGTATATGAAAGAGAAGGTTC
TTATAAAGTATCGTTAAGAGTAAAAGATGATAAAGGCAAAGAGAGTAGAAGCGAAACAACTGTTACGATTAAAGATGGAA
GTTTAACAGAATCAGAACCAAATAATCGTCCAGAGGAAGCAAATCGTATCGGGCTAAATAGTACGATAAAAGGTAATCTT
ATTGGCGGGGACCACACTGATGTTTATACATTTAATGTAGCATCAGCGAAAGATATCGACATTTCTGTTTTAAATGAGTA
TGGAATTGGGATGACATGGGTACTTCACCATGAATCAGATATGCAAAATTATGCAGCTTACGGTCAAGCCAATGGGAATC
ATATAGAAGCGAAATTTAATGCAAAACCAGGCAAGTATTACTTGTATGTATATAAATATGATAATGGCGATGGAACGTAC
TCATTATCAGTAAAGTGA

Upstream 100 bases:

>100_bases
AATTATAGGAAGAAATAATATGAAATATTGCATTTTATATTGTTACATCCAGATTTTATGTTAAATTCTTGAATGGAATA
TTAAAATAGGGGGCAAGGAT

Downstream 100 bases:

>100_bases
AACTTTAATCATTCATCCTCCAATGATTATTAGCCCACACCAATCGGGGGAAAATAAGTGCTTTTCCAATGTATGTTTAA
CTTTACTTTTTGTGGAAAAT

Product: putative microbial collagenase

Products: NA

Alternate protein names: 120 kDa collagenase [H]

Number of amino acids: Translated: 965; Mature: 965

Protein sequence:

>965_residues
MNKKSKINKVMLSISTMALSLGALQTHAAAEEKVPYNVLKTKPVGIEKSVDEVGHISKVDETLSFQERLKVGDFSQRPAS
ITKKTAVKQVKESYSMADLNKMNDQELVETLGSIKWHQITDLFQFNEDAKAFYKDKGKMQVIIDELAHRGSTFTKDDSRG
IQTFTEVLRSAFYLAFYNSELSDLNERSFQDKCLPALKAIAKNPNFKLGTVEQDTVVSAYGKLISNASSDVETVQYASNI
LKQYNDNYTTYVNDRMKGQAIYDIMQGIDYDMQSYLTEARKEANETMWYGKVDGFINEINRIALLNEVTPENKWLVNNGI
YFASRLGKFHSNPNKGLEVVTQAMHMYPRLSEPYFVAVEQITTNYNGKDYSGNTVDLEKIRKEGKEQYLPKTYTFDDGSI
VFKTGDKVSEEKIKRLYWAAKEVKAQYHRVIGNDKALEPGNADDVLTIVIYNSPDEYQLNRQLYGYETNNGGIYIEETGT
FFTYERTPEQSIYSLEELFRHEFTHYLQGRYEVPGLFGRGDMYQNERLTWFQEGNAEFFAGSTRTNNVVPRKSIISGLSS
DPASRYTAERTLFAKYGSWDFYNYSFALQSYLYTHQFETFDKIQDLIRANDVKNYDAYRENLSKDPKLNKEYQEYMQQLI
DNQDKYNVPEVADDYLAEHAPKSLTEVKKEISDTLPMKDTKMTKHNSQFFNTFTLEGTYTGSVTKGESEDWKAMSKRVNE
SLEQLAQKEWSGYKTVTAYFVNYRVNSSNEFEYDVVFHGIAKDDGENKAPTVNINGPYSGLVKEGIQFKSDGSNDEDGKI
VSYLWEFGDGSTSVEVNPVHVYEREGSYKVSLRVKDDKGKESRSETTVTIKDGSLTESEPNNRPEEANRIGLNSTIKGNL
IGGDHTDVYTFNVASAKDIDISVLNEYGIGMTWVLHHESDMQNYAAYGQANGNHIEAKFNAKPGKYYLYVYKYDNGDGTY
SLSVK

Sequences:

>Translated_965_residues
MNKKSKINKVMLSISTMALSLGALQTHAAAEEKVPYNVLKTKPVGIEKSVDEVGHISKVDETLSFQERLKVGDFSQRPAS
ITKKTAVKQVKESYSMADLNKMNDQELVETLGSIKWHQITDLFQFNEDAKAFYKDKGKMQVIIDELAHRGSTFTKDDSRG
IQTFTEVLRSAFYLAFYNSELSDLNERSFQDKCLPALKAIAKNPNFKLGTVEQDTVVSAYGKLISNASSDVETVQYASNI
LKQYNDNYTTYVNDRMKGQAIYDIMQGIDYDMQSYLTEARKEANETMWYGKVDGFINEINRIALLNEVTPENKWLVNNGI
YFASRLGKFHSNPNKGLEVVTQAMHMYPRLSEPYFVAVEQITTNYNGKDYSGNTVDLEKIRKEGKEQYLPKTYTFDDGSI
VFKTGDKVSEEKIKRLYWAAKEVKAQYHRVIGNDKALEPGNADDVLTIVIYNSPDEYQLNRQLYGYETNNGGIYIEETGT
FFTYERTPEQSIYSLEELFRHEFTHYLQGRYEVPGLFGRGDMYQNERLTWFQEGNAEFFAGSTRTNNVVPRKSIISGLSS
DPASRYTAERTLFAKYGSWDFYNYSFALQSYLYTHQFETFDKIQDLIRANDVKNYDAYRENLSKDPKLNKEYQEYMQQLI
DNQDKYNVPEVADDYLAEHAPKSLTEVKKEISDTLPMKDTKMTKHNSQFFNTFTLEGTYTGSVTKGESEDWKAMSKRVNE
SLEQLAQKEWSGYKTVTAYFVNYRVNSSNEFEYDVVFHGIAKDDGENKAPTVNINGPYSGLVKEGIQFKSDGSNDEDGKI
VSYLWEFGDGSTSVEVNPVHVYEREGSYKVSLRVKDDKGKESRSETTVTIKDGSLTESEPNNRPEEANRIGLNSTIKGNL
IGGDHTDVYTFNVASAKDIDISVLNEYGIGMTWVLHHESDMQNYAAYGQANGNHIEAKFNAKPGKYYLYVYKYDNGDGTY
SLSVK
>Mature_965_residues
MNKKSKINKVMLSISTMALSLGALQTHAAAEEKVPYNVLKTKPVGIEKSVDEVGHISKVDETLSFQERLKVGDFSQRPAS
ITKKTAVKQVKESYSMADLNKMNDQELVETLGSIKWHQITDLFQFNEDAKAFYKDKGKMQVIIDELAHRGSTFTKDDSRG
IQTFTEVLRSAFYLAFYNSELSDLNERSFQDKCLPALKAIAKNPNFKLGTVEQDTVVSAYGKLISNASSDVETVQYASNI
LKQYNDNYTTYVNDRMKGQAIYDIMQGIDYDMQSYLTEARKEANETMWYGKVDGFINEINRIALLNEVTPENKWLVNNGI
YFASRLGKFHSNPNKGLEVVTQAMHMYPRLSEPYFVAVEQITTNYNGKDYSGNTVDLEKIRKEGKEQYLPKTYTFDDGSI
VFKTGDKVSEEKIKRLYWAAKEVKAQYHRVIGNDKALEPGNADDVLTIVIYNSPDEYQLNRQLYGYETNNGGIYIEETGT
FFTYERTPEQSIYSLEELFRHEFTHYLQGRYEVPGLFGRGDMYQNERLTWFQEGNAEFFAGSTRTNNVVPRKSIISGLSS
DPASRYTAERTLFAKYGSWDFYNYSFALQSYLYTHQFETFDKIQDLIRANDVKNYDAYRENLSKDPKLNKEYQEYMQQLI
DNQDKYNVPEVADDYLAEHAPKSLTEVKKEISDTLPMKDTKMTKHNSQFFNTFTLEGTYTGSVTKGESEDWKAMSKRVNE
SLEQLAQKEWSGYKTVTAYFVNYRVNSSNEFEYDVVFHGIAKDDGENKAPTVNINGPYSGLVKEGIQFKSDGSNDEDGKI
VSYLWEFGDGSTSVEVNPVHVYEREGSYKVSLRVKDDKGKESRSETTVTIKDGSLTESEPNNRPEEANRIGLNSTIKGNL
IGGDHTDVYTFNVASAKDIDISVLNEYGIGMTWVLHHESDMQNYAAYGQANGNHIEAKFNAKPGKYYLYVYKYDNGDGTY
SLSVK

Specific function: Unknown

COG id: COG3291

COG function: function code R; FOG: PKD repeat

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PKD domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013510
- InterPro:   IPR007280
- InterPro:   IPR002169
- InterPro:   IPR013661
- InterPro:   IPR022409
- InterPro:   IPR000601 [H]

Pfam domain/function: PF01752 Peptidase_M9; PF08453 Peptidase_M9_N; PF00801 PKD; PF04151 PPC [H]

EC number: =3.4.24.3 [H]

Molecular weight: Translated: 110161; Mature: 110161

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS50093 PKD ; PS00142 ZINC_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKSKINKVMLSISTMALSLGALQTHAAAEEKVPYNVLKTKPVGIEKSVDEVGHISKVD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHEECCCCCCCCCHHHHHHHHHHH
ETLSFQERLKVGDFSQRPASITKKTAVKQVKESYSMADLNKMNDQELVETLGSIKWHQIT
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHCCCHHHHH
DLFQFNEDAKAFYKDKGKMQVIIDELAHRGSTFTKDDSRGIQTFTEVLRSAFYLAFYNSE
HHHHCCCHHHHHHHCCCCEEHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHEEEEECCH
LSDLNERSFQDKCLPALKAIAKNPNFKLGTVEQDTVVSAYGKLISNASSDVETVQYASNI
HHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHH
LKQYNDNYTTYVNDRMKGQAIYDIMQGIDYDMQSYLTEARKEANETMWYGKVDGFINEIN
HHHHCCCCEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEECHHHHHHHHH
RIALLNEVTPENKWLVNNGIYFASRLGKFHSNPNKGLEVVTQAMHMYPRLSEPYFVAVEQ
HHHEECCCCCCCCEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEE
ITTNYNGKDYSGNTVDLEKIRKEGKEQYLPKTYTFDDGSIVFKTGDKVSEEKIKRLYWAA
HHCCCCCCCCCCCEEEHHHHHHHHHHHCCCCEEEECCCEEEEECCCCCHHHHHHHHHHHH
KEVKAQYHRVIGNDKALEPGNADDVLTIVIYNSPDEYQLNRQLYGYETNNGGIYIEETGT
HHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCEEECCEEEEEEECCCCEEEEECCC
FFTYERTPEQSIYSLEELFRHEFTHYLQGRYEVPGLFGRGDMYQNERLTWFQEGNAEFFA
EEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEE
GSTRTNNVVPRKSIISGLSSDPASRYTAERTLFAKYGSWDFYNYSFALQSYLYTHQFETF
CCCCCCCCCCHHHHHHCCCCCCHHHHHHHHEEEEECCCCCEEEHHHHHHHHHHHHHHHHH
DKIQDLIRANDVKNYDAYRENLSKDPKLNKEYQEYMQQLIDNQDKYNVPEVADDYLAEHA
HHHHHHHHHCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHC
PKSLTEVKKEISDTLPMKDTKMTKHNSQFFNTFTLEGTYTGSVTKGESEDWKAMSKRVNE
CHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEEEEECCCCCCCCHHHHHHHHHHHH
SLEQLAQKEWSGYKTVTAYFVNYRVNSSNEFEYDVVFHGIAKDDGENKAPTVNINGPYSG
HHHHHHHHHCCCCEEEEEEEEEEEECCCCCEEEEEEEEEECCCCCCCCCCEEEECCCHHH
LVKEGIQFKSDGSNDEDGKIVSYLWEFGDGSTSVEVNPVHVYEREGSYKVSLRVKDDKGK
HHHHCCEECCCCCCCCCCHHHHHHHHCCCCCCEEEECEEEEEECCCCEEEEEEEECCCCC
ESRSETTVTIKDGSLTESEPNNRPEEANRIGLNSTIKGNLIGGDHTDVYTFNVASAKDID
CCCCCEEEEEECCCCCCCCCCCCCCHHHHCCCCCCEECCEECCCCCEEEEEECCCCCCCE
ISVLNEYGIGMTWVLHHESDMQNYAAYGQANGNHIEAKFNAKPGKYYLYVYKYDNGDGTY
EEEECCCCCCEEEEEECCCCHHHHHHCCCCCCCEEEEEECCCCCEEEEEEEEEECCCCEE
SLSVK
EEEEC
>Mature Secondary Structure
MNKKSKINKVMLSISTMALSLGALQTHAAAEEKVPYNVLKTKPVGIEKSVDEVGHISKVD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHEECCCCCCCCCHHHHHHHHHHH
ETLSFQERLKVGDFSQRPASITKKTAVKQVKESYSMADLNKMNDQELVETLGSIKWHQIT
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHCCCHHHHH
DLFQFNEDAKAFYKDKGKMQVIIDELAHRGSTFTKDDSRGIQTFTEVLRSAFYLAFYNSE
HHHHCCCHHHHHHHCCCCEEHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHEEEEECCH
LSDLNERSFQDKCLPALKAIAKNPNFKLGTVEQDTVVSAYGKLISNASSDVETVQYASNI
HHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHH
LKQYNDNYTTYVNDRMKGQAIYDIMQGIDYDMQSYLTEARKEANETMWYGKVDGFINEIN
HHHHCCCCEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEECHHHHHHHHH
RIALLNEVTPENKWLVNNGIYFASRLGKFHSNPNKGLEVVTQAMHMYPRLSEPYFVAVEQ
HHHEECCCCCCCCEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEE
ITTNYNGKDYSGNTVDLEKIRKEGKEQYLPKTYTFDDGSIVFKTGDKVSEEKIKRLYWAA
HHCCCCCCCCCCCEEEHHHHHHHHHHHCCCCEEEECCCEEEEECCCCCHHHHHHHHHHHH
KEVKAQYHRVIGNDKALEPGNADDVLTIVIYNSPDEYQLNRQLYGYETNNGGIYIEETGT
HHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCEEECCEEEEEEECCCCEEEEECCC
FFTYERTPEQSIYSLEELFRHEFTHYLQGRYEVPGLFGRGDMYQNERLTWFQEGNAEFFA
EEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEE
GSTRTNNVVPRKSIISGLSSDPASRYTAERTLFAKYGSWDFYNYSFALQSYLYTHQFETF
CCCCCCCCCCHHHHHHCCCCCCHHHHHHHHEEEEECCCCCEEEHHHHHHHHHHHHHHHHH
DKIQDLIRANDVKNYDAYRENLSKDPKLNKEYQEYMQQLIDNQDKYNVPEVADDYLAEHA
HHHHHHHHHCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHC
PKSLTEVKKEISDTLPMKDTKMTKHNSQFFNTFTLEGTYTGSVTKGESEDWKAMSKRVNE
CHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEEEEECCCCCCCCHHHHHHHHHHHH
SLEQLAQKEWSGYKTVTAYFVNYRVNSSNEFEYDVVFHGIAKDDGENKAPTVNINGPYSG
HHHHHHHHHCCCCEEEEEEEEEEEECCCCCEEEEEEEEEECCCCCCCCCCEEEECCCHHH
LVKEGIQFKSDGSNDEDGKIVSYLWEFGDGSTSVEVNPVHVYEREGSYKVSLRVKDDKGK
HHHHCCEECCCCCCCCCCHHHHHHHHCCCCCCEEEECEEEEEECCCCEEEEEEEECCCCC
ESRSETTVTIKDGSLTESEPNNRPEEANRIGLNSTIKGNLIGGDHTDVYTFNVASAKDID
CCCCCEEEEEECCCCCCCCCCCCCCHHHHCCCCCCEECCEECCCCCEEEEEECCCCCCCE
ISVLNEYGIGMTWVLHHESDMQNYAAYGQANGNHIEAKFNAKPGKYYLYVYKYDNGDGTY
EEEECCCCCCEEEEEECCCCHHHHHHCCCCCCCEEEEEECCCCCEEEEEEEEEECCCCEE
SLSVK
EEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8282691; 11792842 [H]