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The map label for this gene is odhB [H]

Identifier: 218231840

GI number: 218231840

Start: 1274658

End: 1275917

Strand: Reverse

Name: odhB [H]

Synonym: BCB4264_A1313

Alternate gene names: 218231840

Gene position: 1275917-1274658 (Counterclockwise)

Preceding gene: 218232802

Following gene: 218233970

Centisome position: 23.55

GC content: 41.11

Gene sequence:

>1260_bases
ATGATCGAAATTAAAGTACCTGAGCTTGCAGAATCTATTACTGAAGGAACTATTTCACAATGGCTTATCAACGTAGGCGA
CAAAGTTGAGAAAGGTGGCAGCGTTGTTGAGCTTGAAACTGATAAAGTCAATGTAGAAATCATTGCAGAAGATTCAGGTA
TTGTATCGAAGTTACTAGGCGAACCTGGGGATACAGTTGAAGTTGGCGCAACTATCGCAATTTTAGATGCTAATGGAGCA
CCAGTTGCAGTAAGTACACCTGCACCGGCTGCTGAGCAACCAAAACAAGAAACAGCTGAAGCACCGAAAGCTGCGGCACC
AAGTGCTGAGCAAACTGCAACACTACAAGGGTTACCAAATACAAACCGTCCTATCGCATCACCAGCTGCTAGAAAGATGG
CTCGTGAATTAGGAATCGACTTAAACGACGTACGTAGTACAGATCCACTTGGACGCGTGAGACCACATGATGTACAAGCT
CATGCTGCAGCACCAAAAGAAGCACCAGCTGCTCCAAAGAGTCCAGCTCCTGCTCCAGTTGCAAAAACGGAATTCGAAAA
ACCAGTTGAGCGCGTGAAAATGTCCCGCCGCCGTCAAACAATTGCAAAACGTCTTGTAGAAGTTCAACAAACATCTGCAA
TGTTAACAACATTTAACGAAGTTGATATGAGTGCGATCATGGAATTACGTAAAGAACGCAAAGATGCTTTCGAGAAAAAA
CATGATGTACGTCTTGGCTTCATGTCATTCTTCACAAAAGCAGTTGTTGCAGCATTAAAACAATTCCCATTATTAAATGC
TGAAATTCAAGGTGACGAGCTTATCATTAAAAAATTCTATGATATCGGTATTGCAGTAGCAGCTCCAGATGGATTAGTTG
TTCCAGTTGTACGCGATGCTAACCAATTAAACTTCGCTGAAATCGAAAGCGAAATTCGTGAATTAGGTAAAAAAGCACGC
GATAACAAACTTTCATTAAAAGAACTACAAGGTGGTACATTTACAATTACAAACGGTGGTGTATTCGGTTCTCTAATGTC
AACACCAATCCTAAATAGCCCTCAAGTTGGTATTTTAGGAATGCATAAAATCCAAGTACGCCCAGTTGCAATTGATAACG
AGCGTATGGAAAACCGCCCAATGATGTACATTGCTTTATCTTACGATCACCGTATTGTTGATGGTAAAGAAGCAGTTAGC
TTCCTTGTTGCTGTTAAAGATATGCTTGAAGATCCAAAGTCATTATTATTAGAAGGTTGA

Upstream 100 bases:

>100_bases
GGGCAGATATTCTGCCCAAGGCCAGGCTAACAAATGGAGATTACCGAAGACAAAAGAAGAAAAAACACACCGTTAGGCAA
ATAAGGGGAGGACATTTAAA

Downstream 100 bases:

>100_bases
TAGATTAAAAATCTATTAAAGGCTGTAGAATGAATCATTCTACAGCCTTTTTTTATTGTGTTCATACGGGACAAATTTCA
TGACATAAAGTAATAATGTA

Product: dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MIEIKVPELAESITEGTISQWLINVGDKVEKGGSVVELETDKVNVEIIAEDSGIVSKLLGEPGDTVEVGATIAILDANGA
PVAVSTPAPAAEQPKQETAEAPKAAAPSAEQTATLQGLPNTNRPIASPAARKMARELGIDLNDVRSTDPLGRVRPHDVQA
HAAAPKEAPAAPKSPAPAPVAKTEFEKPVERVKMSRRRQTIAKRLVEVQQTSAMLTTFNEVDMSAIMELRKERKDAFEKK
HDVRLGFMSFFTKAVVAALKQFPLLNAEIQGDELIIKKFYDIGIAVAAPDGLVVPVVRDANQLNFAEIESEIRELGKKAR
DNKLSLKELQGGTFTITNGGVFGSLMSTPILNSPQVGILGMHKIQVRPVAIDNERMENRPMMYIALSYDHRIVDGKEAVS
FLVAVKDMLEDPKSLLLEG

Sequences:

>Translated_419_residues
MIEIKVPELAESITEGTISQWLINVGDKVEKGGSVVELETDKVNVEIIAEDSGIVSKLLGEPGDTVEVGATIAILDANGA
PVAVSTPAPAAEQPKQETAEAPKAAAPSAEQTATLQGLPNTNRPIASPAARKMARELGIDLNDVRSTDPLGRVRPHDVQA
HAAAPKEAPAAPKSPAPAPVAKTEFEKPVERVKMSRRRQTIAKRLVEVQQTSAMLTTFNEVDMSAIMELRKERKDAFEKK
HDVRLGFMSFFTKAVVAALKQFPLLNAEIQGDELIIKKFYDIGIAVAAPDGLVVPVVRDANQLNFAEIESEIRELGKKAR
DNKLSLKELQGGTFTITNGGVFGSLMSTPILNSPQVGILGMHKIQVRPVAIDNERMENRPMMYIALSYDHRIVDGKEAVS
FLVAVKDMLEDPKSLLLEG
>Mature_419_residues
MIEIKVPELAESITEGTISQWLINVGDKVEKGGSVVELETDKVNVEIIAEDSGIVSKLLGEPGDTVEVGATIAILDANGA
PVAVSTPAPAAEQPKQETAEAPKAAAPSAEQTATLQGLPNTNRPIASPAARKMARELGIDLNDVRSTDPLGRVRPHDVQA
HAAAPKEAPAAPKSPAPAPVAKTEFEKPVERVKMSRRRQTIAKRLVEVQQTSAMLTTFNEVDMSAIMELRKERKDAFEKK
HDVRLGFMSFFTKAVVAALKQFPLLNAEIQGDELIIKKFYDIGIAVAAPDGLVVPVVRDANQLNFAEIESEIRELGKKAR
DNKLSLKELQGGTFTITNGGVFGSLMSTPILNSPQVGILGMHKIQVRPVAIDNERMENRPMMYIALSYDHRIVDGKEAVS
FLVAVKDMLEDPKSLLLEG

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=231, Percent_Identity=57.1428571428571, Blast_Score=271, Evalue=1e-72,
Organism=Homo sapiens, GI31711992, Length=437, Percent_Identity=30.2059496567506, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI203098753, Length=456, Percent_Identity=29.6052631578947, Blast_Score=169, Evalue=4e-42,
Organism=Homo sapiens, GI203098816, Length=456, Percent_Identity=29.6052631578947, Blast_Score=169, Evalue=4e-42,
Organism=Homo sapiens, GI110671329, Length=438, Percent_Identity=26.7123287671233, Blast_Score=152, Evalue=4e-37,
Organism=Homo sapiens, GI260898739, Length=168, Percent_Identity=35.7142857142857, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI1786946, Length=417, Percent_Identity=45.083932853717, Blast_Score=342, Evalue=3e-95,
Organism=Escherichia coli, GI1786305, Length=426, Percent_Identity=34.037558685446, Blast_Score=211, Evalue=5e-56,
Organism=Caenorhabditis elegans, GI25146366, Length=424, Percent_Identity=40.5660377358491, Blast_Score=283, Evalue=1e-76,
Organism=Caenorhabditis elegans, GI17537937, Length=434, Percent_Identity=29.9539170506912, Blast_Score=168, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI17560088, Length=439, Percent_Identity=29.6127562642369, Blast_Score=164, Evalue=6e-41,
Organism=Caenorhabditis elegans, GI17538894, Length=265, Percent_Identity=31.6981132075472, Blast_Score=115, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6320352, Length=414, Percent_Identity=42.9951690821256, Blast_Score=321, Evalue=2e-88,
Organism=Saccharomyces cerevisiae, GI6324258, Length=455, Percent_Identity=29.8901098901099, Blast_Score=170, Evalue=4e-43,
Organism=Drosophila melanogaster, GI24645909, Length=223, Percent_Identity=54.2600896860987, Blast_Score=255, Evalue=4e-68,
Organism=Drosophila melanogaster, GI18859875, Length=429, Percent_Identity=26.3403263403263, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI20129315, Length=233, Percent_Identity=30.9012875536481, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24582497, Length=233, Percent_Identity=30.9012875536481, Blast_Score=112, Evalue=3e-25,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 45328; Mature: 45328

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEIKVPELAESITEGTISQWLINVGDKVEKGGSVVELETDKVNVEIIAEDSGIVSKLLG
CEEEECHHHHHHHHHHHHHHHHHHCCCHHHCCCCEEEEECCEEEEEEEECCCHHHHHHHC
EPGDTVEVGATIAILDANGAPVAVSTPAPAAEQPKQETAEAPKAAAPSAEQTATLQGLPN
CCCCEEEECEEEEEEECCCCCEEEECCCCCCCCCHHHHHCCCCCCCCCCHHCEEECCCCC
TNRPIASPAARKMARELGIDLNDVRSTDPLGRVRPHDVQAHAAAPKEAPAAPKSPAPAPV
CCCCCCCHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCC
AKTEFEKPVERVKMSRRRQTIAKRLVEVQQTSAMLTTFNEVDMSAIMELRKERKDAFEKK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HDVRLGFMSFFTKAVVAALKQFPLLNAEIQGDELIIKKFYDIGIAVAAPDGLVVPVVRDA
HCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCEEEECCCCEEEEEECCC
NQLNFAEIESEIRELGKKARDNKLSLKELQGGTFTITNGGVFGSLMSTPILNSPQVGILG
CCCCHHHHHHHHHHHHHHHCCCCCCHHHCCCCEEEEECCCHHHHHHHCCCCCCCCCCEEE
MHKIQVRPVAIDNERMENRPMMYIALSYDHRIVDGKEAVSFLVAVKDMLEDPKSLLLEG
EEEEEEEEEEECCCHHCCCCEEEEEEECCCEECCHHHHHHHHHHHHHHHHCCHHHHCCC
>Mature Secondary Structure
MIEIKVPELAESITEGTISQWLINVGDKVEKGGSVVELETDKVNVEIIAEDSGIVSKLLG
CEEEECHHHHHHHHHHHHHHHHHHCCCHHHCCCCEEEEECCEEEEEEEECCCHHHHHHHC
EPGDTVEVGATIAILDANGAPVAVSTPAPAAEQPKQETAEAPKAAAPSAEQTATLQGLPN
CCCCEEEECEEEEEEECCCCCEEEECCCCCCCCCHHHHHCCCCCCCCCCHHCEEECCCCC
TNRPIASPAARKMARELGIDLNDVRSTDPLGRVRPHDVQAHAAAPKEAPAAPKSPAPAPV
CCCCCCCHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCC
AKTEFEKPVERVKMSRRRQTIAKRLVEVQQTSAMLTTFNEVDMSAIMELRKERKDAFEKK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HDVRLGFMSFFTKAVVAALKQFPLLNAEIQGDELIIKKFYDIGIAVAAPDGLVVPVVRDA
HCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCEEEECCCCEEEEEECCC
NQLNFAEIESEIRELGKKARDNKLSLKELQGGTFTITNGGVFGSLMSTPILNSPQVGILG
CCCCHHHHHHHHHHHHHHHCCCCCCHHHCCCCEEEEECCCHHHHHHHCCCCCCCCCCEEE
MHKIQVRPVAIDNERMENRPMMYIALSYDHRIVDGKEAVSFLVAVKDMLEDPKSLLLEG
EEEEEEEEEEECCCHHCCCCEEEEEEECCCEECCHHHHHHHHHHHHHHHHCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2500417; 9384377 [H]