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The map label for this gene is pbpB [H]

Identifier: 218231650

GI number: 218231650

Start: 3940936

End: 3943086

Strand: Reverse

Name: pbpB [H]

Synonym: BCB4264_A4017

Alternate gene names: 218231650

Gene position: 3943086-3940936 (Counterclockwise)

Preceding gene: 218235302

Following gene: 218231282

Centisome position: 72.76

GC content: 38.21

Gene sequence:

>2151_bases
ATGAAGAAGAATATGACTAAATTTCATACCAATAAGGGAGCAGGTTATTTTATGATATTTTTCCTCCTGCTCTTTTTGCT
GTTATTAGCCCGATTTTTTTACATACAATCAACAGGAACAGTTCATAATCAGGATTTAGATGCACTTGCTAAACAAAAGC
ATAGTAAAAAAGGAGTTCTTGAAGCGAACCGAGGAACCATTTATGATCAAAACGGTCATGTGTTAGCGCAAGACGCAAAC
TCTTACAAAATGGTAGCTGCACTAAAAGGTGCTAACTCAGTTGAGAATAAAGAAGATACTGCAAAGAAAATTGCAGGAGT
ACTTGGAAAAGGCGAAGAGGATATTTTAGCTTCTTTAAACAAGGAAGGTAGAAGTCAAGTCGAATTTGGAACGTTAGGCA
AGGATTTGACGAAAGAAAAGAAGGAACAAATAGAAGCGTTGAAGTTGCCGGGAATATCTTTTATTACAGAAAATGCAAGG
GTGTATCCAAACGGTGATTTTGCATCTTACGTTATAGGTCATGCAAAACCAAATGAGAATGGAACCTCGGTAGGTCAATT
TGGCATAGAGAAAAGCTTGGATAAATACTTAAGTGCTTCGAACGGAGAAGTAGCATATACAGGTGATCGTAAAGGTGTGT
CCCTTGATGGGGGAAAAGTTAATGTAAAGGCACCTAAAAATGGAGATAACGCTTATTTAACAATTGATCAACGTGTCCAA
AGTTATTTAGAAGACGCGATGAAAGCAGCAAGTAAGCAATATGAGCCAGAAAGTTTAATAGGGATTGTTGCAGATCCGAA
AACTGGGAAAATATTAGCGATGTCTACTAAACCTAGTTATGACCCTAATGATCGCCAAATTAAATATTTCTTCAATGACG
CGATTGCCAATGCATTTGAACCTGGATCAACAATGAAAATTTTTACGTTAGCGGCAGCTATTAATGAAGGTGTGTACAAA
GGACAAGATTATTATCAATCCGGGACATATCAAGTCGGAAATAGGAAAATAAAAGATCATAATGGCGGTGCTGGTTGGGG
CTCTATCACGTTTGATGAAGGGGTAGAGCGTTCTTCGAACGTAGCGTTTGCAATTTTAGGAGATCAAAAACTTGGTCCAG
AACGTTTCCGAAAATATATTCATAGTTTTGGATTAGATGAAAAAACGAACATTGATTTATCAGGTGAAGGATCGAATACA
ATTTTATTTGATCAGCAAATACAACAAGTGACAACAGCTTTTGGACAAGGTTCCACTGTAACACCAATTCAGCTTGTACA
AGCTGCAACGGCCATTGCAAATGATGGGAAAATGATGAAACCTTATGCAATTGATAAAATTGTAGATCCGATAACAGACA
AAGTAAAATTAGAACATAAGCCAGAAGAAGTAGGAAAACCTGTTACAAAGGAAACAGCAGCGCAAGTAAGACAGTTATTA
GAACGTGTTGTTACATCTCCGAAAGGTACAGGGACAGCTTATAAAATTGATGGATATTCAGTTGGTGGTAAAACAGGTAC
AGCGCAAATTCCGGATGGAAAAGGCGGCTATATGACAGGAAGGCAGAATTATATATTCTCATTCTTAGGTATGGCACCAA
TGGACGATCCACAACTTGTCGTGTATGTAGCTGTTAAACAGCCGAAATTAAAAGATGATGAGAACGGCGCACAACCTTTA
GCTGATATTTTTAAATATGTAACAAAAAATAGTTTAGAGTATTTAAAGATTAAGCCAAATGAAGTGAAAGATCCGAAAAA
ACATCTGAAAGAACAACAAACTACTGTTCCAGATGTAACTGGTAAAACGATGGCTGAAGCGGGGAAAGCGATTGATAAAG
CAAAACTTCGTCCAATCGTACTAGGTGAAGGGAAAGTACAGCAACAAGTACCAAAAGCGACTGAACAAACATTAAAAGGC
GACCGAGTCTTCTTGGTAGGAGATAAGCCTACAATGCCAAATATACAAGGATGGGCACTGCGTGATGTTATGAATTTAGC
GAAGACATTACAGCTTAACTTGAAACCTTCTGGTACAGGATACGTAACAGAACAAAGTGTGGCGGAAGGAACATTATTAC
AACCTGGTACAGAATTAGGTGTCACACTCGTACCGCCACTCGAACCACAGCAAGAAGCAGAAAAGCCGTAA

Upstream 100 bases:

>100_bases
TCGCTATGAACGAATCGCTGAAGTTGCAAAAAAACATGGGCTAGAAATTAATGCGAATAATGTGAAAGGCCTCAAATAAG
GCAATAGGTGTGAAGGAACG

Downstream 100 bases:

>100_bases
TGGTAAAAGAGAATAAGTTCTAATTAAATAAGTACAAGCATATATTGGAACAAGCTAGGCGTAAGGGAGGCTTGTTCCGA
TATGCGTGTATCAAATGTAA

Product: penicillin-binding protein

Products: NA

Alternate protein names: PBP-2B; Penicillin-binding protein B [H]

Number of amino acids: Translated: 716; Mature: 716

Protein sequence:

>716_residues
MKKNMTKFHTNKGAGYFMIFFLLLFLLLLARFFYIQSTGTVHNQDLDALAKQKHSKKGVLEANRGTIYDQNGHVLAQDAN
SYKMVAALKGANSVENKEDTAKKIAGVLGKGEEDILASLNKEGRSQVEFGTLGKDLTKEKKEQIEALKLPGISFITENAR
VYPNGDFASYVIGHAKPNENGTSVGQFGIEKSLDKYLSASNGEVAYTGDRKGVSLDGGKVNVKAPKNGDNAYLTIDQRVQ
SYLEDAMKAASKQYEPESLIGIVADPKTGKILAMSTKPSYDPNDRQIKYFFNDAIANAFEPGSTMKIFTLAAAINEGVYK
GQDYYQSGTYQVGNRKIKDHNGGAGWGSITFDEGVERSSNVAFAILGDQKLGPERFRKYIHSFGLDEKTNIDLSGEGSNT
ILFDQQIQQVTTAFGQGSTVTPIQLVQAATAIANDGKMMKPYAIDKIVDPITDKVKLEHKPEEVGKPVTKETAAQVRQLL
ERVVTSPKGTGTAYKIDGYSVGGKTGTAQIPDGKGGYMTGRQNYIFSFLGMAPMDDPQLVVYVAVKQPKLKDDENGAQPL
ADIFKYVTKNSLEYLKIKPNEVKDPKKHLKEQQTTVPDVTGKTMAEAGKAIDKAKLRPIVLGEGKVQQQVPKATEQTLKG
DRVFLVGDKPTMPNIQGWALRDVMNLAKTLQLNLKPSGTGYVTEQSVAEGTLLQPGTELGVTLVPPLEPQQEAEKP

Sequences:

>Translated_716_residues
MKKNMTKFHTNKGAGYFMIFFLLLFLLLLARFFYIQSTGTVHNQDLDALAKQKHSKKGVLEANRGTIYDQNGHVLAQDAN
SYKMVAALKGANSVENKEDTAKKIAGVLGKGEEDILASLNKEGRSQVEFGTLGKDLTKEKKEQIEALKLPGISFITENAR
VYPNGDFASYVIGHAKPNENGTSVGQFGIEKSLDKYLSASNGEVAYTGDRKGVSLDGGKVNVKAPKNGDNAYLTIDQRVQ
SYLEDAMKAASKQYEPESLIGIVADPKTGKILAMSTKPSYDPNDRQIKYFFNDAIANAFEPGSTMKIFTLAAAINEGVYK
GQDYYQSGTYQVGNRKIKDHNGGAGWGSITFDEGVERSSNVAFAILGDQKLGPERFRKYIHSFGLDEKTNIDLSGEGSNT
ILFDQQIQQVTTAFGQGSTVTPIQLVQAATAIANDGKMMKPYAIDKIVDPITDKVKLEHKPEEVGKPVTKETAAQVRQLL
ERVVTSPKGTGTAYKIDGYSVGGKTGTAQIPDGKGGYMTGRQNYIFSFLGMAPMDDPQLVVYVAVKQPKLKDDENGAQPL
ADIFKYVTKNSLEYLKIKPNEVKDPKKHLKEQQTTVPDVTGKTMAEAGKAIDKAKLRPIVLGEGKVQQQVPKATEQTLKG
DRVFLVGDKPTMPNIQGWALRDVMNLAKTLQLNLKPSGTGYVTEQSVAEGTLLQPGTELGVTLVPPLEPQQEAEKP
>Mature_716_residues
MKKNMTKFHTNKGAGYFMIFFLLLFLLLLARFFYIQSTGTVHNQDLDALAKQKHSKKGVLEANRGTIYDQNGHVLAQDAN
SYKMVAALKGANSVENKEDTAKKIAGVLGKGEEDILASLNKEGRSQVEFGTLGKDLTKEKKEQIEALKLPGISFITENAR
VYPNGDFASYVIGHAKPNENGTSVGQFGIEKSLDKYLSASNGEVAYTGDRKGVSLDGGKVNVKAPKNGDNAYLTIDQRVQ
SYLEDAMKAASKQYEPESLIGIVADPKTGKILAMSTKPSYDPNDRQIKYFFNDAIANAFEPGSTMKIFTLAAAINEGVYK
GQDYYQSGTYQVGNRKIKDHNGGAGWGSITFDEGVERSSNVAFAILGDQKLGPERFRKYIHSFGLDEKTNIDLSGEGSNT
ILFDQQIQQVTTAFGQGSTVTPIQLVQAATAIANDGKMMKPYAIDKIVDPITDKVKLEHKPEEVGKPVTKETAAQVRQLL
ERVVTSPKGTGTAYKIDGYSVGGKTGTAQIPDGKGGYMTGRQNYIFSFLGMAPMDDPQLVVYVAVKQPKLKDDENGAQPL
ADIFKYVTKNSLEYLKIKPNEVKDPKKHLKEQQTTVPDVTGKTMAEAGKAIDKAKLRPIVLGEGKVQQQVPKATEQTLKG
DRVFLVGDKPTMPNIQGWALRDVMNLAKTLQLNLKPSGTGYVTEQSVAEGTLLQPGTELGVTLVPPLEPQQEAEKP

Specific function: Penicillin-binding proteins (PBPs) function in the late steps of murein biosynthesis. PBP-2B is required for vegetative cell division and sporulation septation. Beta-lactamase inactivates the PBPs by acylating an essential serine residue in the active sit

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PASTA domains [H]

Homologues:

Organism=Escherichia coli, GI1786272, Length=578, Percent_Identity=27.681660899654, Blast_Score=177, Evalue=2e-45,
Organism=Escherichia coli, GI1786854, Length=547, Percent_Identity=23.400365630713, Blast_Score=115, Evalue=7e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005543
- InterPro:   IPR005311
- InterPro:   IPR001460 [H]

Pfam domain/function: PF03793 PASTA; PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 78089; Mature: 78089

Theoretical pI: Translated: 9.32; Mature: 9.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKNMTKFHTNKGAGYFMIFFLLLFLLLLARFFYIQSTGTVHNQDLDALAKQKHSKKGVL
CCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCE
EANRGTIYDQNGHVLAQDANSYKMVAALKGANSVENKEDTAKKIAGVLGKGEEDILASLN
ECCCCEEEECCCEEEEECCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHC
KEGRSQVEFGTLGKDLTKEKKEQIEALKLPGISFITENARVYPNGDFASYVIGHAKPNEN
CCCCCCEEHHHCCHHHHHHHHHHHHHHCCCCCEEEECCCEEECCCCCHHEEEECCCCCCC
GTSVGQFGIEKSLDKYLSASNGEVAYTGDRKGVSLDGGKVNVKAPKNGDNAYLTIDQRVQ
CCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEECCCEEEEECCCCCCCEEEEEHHHHH
SYLEDAMKAASKQYEPESLIGIVADPKTGKILAMSTKPSYDPNDRQIKYFFNDAIANAFE
HHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEEECCCCCCCCCCEEEEEECHHHHHCCC
PGSTMKIFTLAAAINEGVYKGQDYYQSGTYQVGNRKIKDHNGGAGWGSITFDEGVERSSN
CCCCEEHHHHHHHHHCCCCCCCHHHCCCCEEECCEEEECCCCCCCCCCCCHHHCCCCCCC
VAFAILGDQKLGPERFRKYIHSFGLDEKTNIDLSGEGSNTILFDQQIQQVTTAFGQGSTV
EEEEEECCCCCCHHHHHHHHHHCCCCCCCCEEECCCCCCEEEEHHHHHHHHHHHCCCCCC
TPIQLVQAATAIANDGKMMKPYAIDKIVDPITDKVKLEHKPEEVGKPVTKETAAQVRQLL
CHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHEEECCCHHHHCCCCHHHHHHHHHHHH
ERVVTSPKGTGTAYKIDGYSVGGKTGTAQIPDGKGGYMTGRQNYIFSFLGMAPMDDPQLV
HHHHCCCCCCCCEEEECCEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCEE
VYVAVKQPKLKDDENGAQPLADIFKYVTKNSLEYLKIKPNEVKDPKKHLKEQQTTVPDVT
EEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHCCCCCCC
GKTMAEAGKAIDKAKLRPIVLGEGKVQQQVPKATEQTLKGDRVFLVGDKPTMPNIQGWAL
CHHHHHHHHHHHHHHCCEEEECCCHHHHHCCHHHHHHCCCCEEEEEECCCCCCCCCCHHH
RDVMNLAKTLQLNLKPSGTGYVTEQSVAEGTLLQPGTELGVTLVPPLEPQQEAEKP
HHHHHHHHHEEEEECCCCCCCEECHHHCCCCEECCCCCCCEEEECCCCCHHHCCCC
>Mature Secondary Structure
MKKNMTKFHTNKGAGYFMIFFLLLFLLLLARFFYIQSTGTVHNQDLDALAKQKHSKKGVL
CCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCE
EANRGTIYDQNGHVLAQDANSYKMVAALKGANSVENKEDTAKKIAGVLGKGEEDILASLN
ECCCCEEEECCCEEEEECCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHC
KEGRSQVEFGTLGKDLTKEKKEQIEALKLPGISFITENARVYPNGDFASYVIGHAKPNEN
CCCCCCEEHHHCCHHHHHHHHHHHHHHCCCCCEEEECCCEEECCCCCHHEEEECCCCCCC
GTSVGQFGIEKSLDKYLSASNGEVAYTGDRKGVSLDGGKVNVKAPKNGDNAYLTIDQRVQ
CCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEECCCEEEEECCCCCCCEEEEEHHHHH
SYLEDAMKAASKQYEPESLIGIVADPKTGKILAMSTKPSYDPNDRQIKYFFNDAIANAFE
HHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEEECCCCCCCCCCEEEEEECHHHHHCCC
PGSTMKIFTLAAAINEGVYKGQDYYQSGTYQVGNRKIKDHNGGAGWGSITFDEGVERSSN
CCCCEEHHHHHHHHHCCCCCCCHHHCCCCEEECCEEEECCCCCCCCCCCCHHHCCCCCCC
VAFAILGDQKLGPERFRKYIHSFGLDEKTNIDLSGEGSNTILFDQQIQQVTTAFGQGSTV
EEEEEECCCCCCHHHHHHHHHHCCCCCCCCEEECCCCCCEEEEHHHHHHHHHHHCCCCCC
TPIQLVQAATAIANDGKMMKPYAIDKIVDPITDKVKLEHKPEEVGKPVTKETAAQVRQLL
CHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHEEECCCHHHHCCCCHHHHHHHHHHHH
ERVVTSPKGTGTAYKIDGYSVGGKTGTAQIPDGKGGYMTGRQNYIFSFLGMAPMDDPQLV
HHHHCCCCCCCCEEEECCEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCEE
VYVAVKQPKLKDDENGAQPLADIFKYVTKNSLEYLKIKPNEVKDPKKHLKEQQTTVPDVT
EEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHCCCCCCC
GKTMAEAGKAIDKAKLRPIVLGEGKVQQQVPKATEQTLKGDRVFLVGDKPTMPNIQGWAL
CHHHHHHHHHHHHHHCCEEEECCCHHHHHCCHHHHHHCCCCEEEEEECCCCCCCCCCHHH
RDVMNLAKTLQLNLKPSGTGYVTEQSVAEGTLLQPGTELGVTLVPPLEPQQEAEKP
HHHHHHHHHEEEEECCCCCCCEECHHHCCCCEECCCCCCCEEEECCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8244929; 9384377; 8289242 [H]