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The map label for this gene is exoA [H]

Identifier: 218231608

GI number: 218231608

Start: 3748186

End: 3748944

Strand: Reverse

Name: exoA [H]

Synonym: BCB4264_A3839

Alternate gene names: 218231608

Gene position: 3748944-3748186 (Counterclockwise)

Preceding gene: 218234009

Following gene: 218234327

Centisome position: 69.18

GC content: 33.6

Gene sequence:

>759_bases
GTGAAGTTAATTTCATGGAATGTAAATGGTTTGCGAGCAGTTATCGCAAAAGGTGAATTCTTGGAATATCTCGAGGAATC
AAGTGCTGATATATTTTGTCTACAAGAGATTAAATTACAAGAAGGGCAAATTGATTTAAATCTAGAGGGATATTATACAT
ACTGGAATTATGCTGTGAAAAAAGGATATTCAGGAACTGCTATTTTTTCGAAAAAAGAACCGCTTTCAGTTACATATGGT
TTAGACATTGAAGAGCATGATCAAGAAGGGCGACTTATTACTTTAGAATTCGAAGATTTTTATATGATAACGTTATATAC
ACCAAATTCAAAACGTGGATTAGAACGATTAGATTACAGAATGAAATGGGAAGATGATTTCAGGTCCTATATCAAACGAT
TAGATGAGAAAAAACCAGTTATTTTTTGTGGAGACTTAAATGTTGCTCATAAAGAAATCGATTTGAAAAATCCAAAAAGT
AATCGTAAAAATCCTGGATTCTCTGATGAGGAGCGAGAGAAATTTTCAAGTATTTTAGAAGAAGGATTTATTGATACATA
TCGTTACCTTTATCCTGATCAAGAAGGTGCATACTCGTGGTGGTCGTATCGTATGGGCGCGAGAGCGAAAAATATCGGAT
GGCGTTTAGATTATTTTGTTGTTTCGGAACGAATGAAAGACCAAATAACAGAAGCGAAAATTAATAGTGAAGTGATGGGA
TCAGACCATTGTCCAGTTGAATTGCATATAAATTTCTAA

Upstream 100 bases:

>100_bases
CATAAATGTAAGAGCAAGATTACAAAGGTGAAAATATGATTGTTTTTCTATTTTGTGATAGCATGAAATTTGGAAATAAA
CATCAAGAGGAGGGCTAAAA

Downstream 100 bases:

>100_bases
AAAAAGAAGTATCTCCCTAATATCAGGGAGATACTTCTTTTTTAATTCAATTTGTTAAAATTTTTCAAATCATTCTTGAA
ATAATTTCATATTAAATATA

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MKLISWNVNGLRAVIAKGEFLEYLEESSADIFCLQEIKLQEGQIDLNLEGYYTYWNYAVKKGYSGTAIFSKKEPLSVTYG
LDIEEHDQEGRLITLEFEDFYMITLYTPNSKRGLERLDYRMKWEDDFRSYIKRLDEKKPVIFCGDLNVAHKEIDLKNPKS
NRKNPGFSDEEREKFSSILEEGFIDTYRYLYPDQEGAYSWWSYRMGARAKNIGWRLDYFVVSERMKDQITEAKINSEVMG
SDHCPVELHINF

Sequences:

>Translated_252_residues
MKLISWNVNGLRAVIAKGEFLEYLEESSADIFCLQEIKLQEGQIDLNLEGYYTYWNYAVKKGYSGTAIFSKKEPLSVTYG
LDIEEHDQEGRLITLEFEDFYMITLYTPNSKRGLERLDYRMKWEDDFRSYIKRLDEKKPVIFCGDLNVAHKEIDLKNPKS
NRKNPGFSDEEREKFSSILEEGFIDTYRYLYPDQEGAYSWWSYRMGARAKNIGWRLDYFVVSERMKDQITEAKINSEVMG
SDHCPVELHINF
>Mature_252_residues
MKLISWNVNGLRAVIAKGEFLEYLEESSADIFCLQEIKLQEGQIDLNLEGYYTYWNYAVKKGYSGTAIFSKKEPLSVTYG
LDIEEHDQEGRLITLEFEDFYMITLYTPNSKRGLERLDYRMKWEDDFRSYIKRLDEKKPVIFCGDLNVAHKEIDLKNPKS
NRKNPGFSDEEREKFSSILEEGFIDTYRYLYPDQEGAYSWWSYRMGARAKNIGWRLDYFVVSERMKDQITEAKINSEVMG
SDHCPVELHINF

Specific function: Major Apurinic-Apyrimidinic Endonuclease Of E.Coli. It Removes The Damaged DNA At Cytosines And Guanines By Cleaving On The 3' Side Of The Ap Site By A Beta-Elimination Reaction. It Exhibits 3'-5'-Exonuclease, 3'-Phosphomonoesterase, 3'-Repair Diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=258, Percent_Identity=46.8992248062016, Blast_Score=268, Evalue=3e-72,
Organism=Homo sapiens, GI18375503, Length=258, Percent_Identity=46.8992248062016, Blast_Score=268, Evalue=3e-72,
Organism=Homo sapiens, GI18375501, Length=258, Percent_Identity=46.8992248062016, Blast_Score=268, Evalue=3e-72,
Organism=Homo sapiens, GI18375507, Length=318, Percent_Identity=31.1320754716981, Blast_Score=115, Evalue=4e-26,
Organism=Escherichia coli, GI1788046, Length=266, Percent_Identity=27.4436090225564, Blast_Score=103, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI71989536, Length=256, Percent_Identity=46.875, Blast_Score=223, Evalue=1e-58,
Organism=Caenorhabditis elegans, GI71989539, Length=145, Percent_Identity=38.6206896551724, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI221330655, Length=255, Percent_Identity=49.8039215686275, Blast_Score=275, Evalue=2e-74,
Organism=Drosophila melanogaster, GI17136678, Length=255, Percent_Identity=49.8039215686275, Blast_Score=275, Evalue=2e-74,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29762; Mature: 29762

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00726 AP_NUCLEASE_F1_1 ; PS00728 AP_NUCLEASE_F1_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLISWNVNGLRAVIAKGEFLEYLEESSADIFCLQEIKLQEGQIDLNLEGYYTYWNYAVK
CEEEEECCCCEEEEECCCHHHHHHHHCCCCEEEEEEEEECCCEEEEEEEEEEEEEEEEEC
KGYSGTAIFSKKEPLSVTYGLDIEEHDQEGRLITLEFEDFYMITLYTPNSKRGLERLDYR
CCCCCCEEEECCCCEEEEEECCCCCCCCCCCEEEEEECCEEEEEEECCCCCCCHHHHCCE
MKWEDDFRSYIKRLDEKKPVIFCGDLNVAHKEIDLKNPKSNRKNPGFSDEEREKFSSILE
ECCCHHHHHHHHHHCCCCCEEEECCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHH
EGFIDTYRYLYPDQEGAYSWWSYRMGARAKNIGWRLDYFVVSERMKDQITEAKINSEVMG
HCCHHHHHHCCCCCCCCCEEEHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCHHCC
SDHCPVELHINF
CCCCCEEEEECC
>Mature Secondary Structure
MKLISWNVNGLRAVIAKGEFLEYLEESSADIFCLQEIKLQEGQIDLNLEGYYTYWNYAVK
CEEEEECCCCEEEEECCCHHHHHHHHCCCCEEEEEEEEECCCEEEEEEEEEEEEEEEEEC
KGYSGTAIFSKKEPLSVTYGLDIEEHDQEGRLITLEFEDFYMITLYTPNSKRGLERLDYR
CCCCCCEEEECCCCEEEEEECCCCCCCCCCCEEEEEECCEEEEEEECCCCCCCHHHHCCE
MKWEDDFRSYIKRLDEKKPVIFCGDLNVAHKEIDLKNPKSNRKNPGFSDEEREKFSSILE
ECCCHHHHHHHHHHCCCCCEEEECCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHH
EGFIDTYRYLYPDQEGAYSWWSYRMGARAKNIGWRLDYFVVSERMKDQITEAKINSEVMG
HCCHHHHHHCCCCCCCCCEEEHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCHHCC
SDHCPVELHINF
CCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]