Click here to switch to the map view.

The map label for this gene is cwlD [H]

Identifier: 218231285

GI number: 218231285

Start: 146303

End: 147016

Strand: Direct

Name: cwlD [H]

Synonym: BCB4264_A0168

Alternate gene names: 218231285

Gene position: 146303-147016 (Clockwise)

Preceding gene: 218233059

Following gene: 218231467

Centisome position: 2.7

GC content: 35.43

Gene sequence:

>714_bases
ATGAAGAGAATTCGAATTATCTCTTTTGCACTCGCTGCGGTTGTATTGTTTTTTTTAGTCAAGCAAGAATTTCAAATTAC
AAAATCGTGGCGAGCTTGGAATTTACCCTTATCAGGGAAAGTAATTGTGTTAGATGCTGGGCATGGTGGACCAGATGGAG
GGGCTGTTGGTGGAAAGGATATTATAGAAAAGGATATTACGCTTGAAATTACAAAAAAGGTACAAGACTATTTACAAGAA
CAAGGTGCACTAGTTATTTTAACGCGTGAGGGAGATTATGATTTAGCTAACAAAGATACAAAGTCATATAGTAGACGTAA
AGCAGAGGATTTAAAAAAACGTGTAGAAATCATCAATAAACCTGATGTAGACTTTTTTGCAAGTATTCATTTAAATGCTT
TAACTAGTAGTGGATCGAAAGGCGCACAAACGTTCTATTACCGCTCTTCGATTGAAAATGAACGTGCTGCAAAATTTATA
CAGGCTGAATTGAGAACAAGTTTAGAGAACACGAATCGTTCTGCTAAGACAATTTCGCATGTATATTTATTAAAGTATTC
TAAAACACCAGGTGCTTTAATTGAAGCTGGCTTTTTATCGAATGTGAACGAAAGGTATATGTTAAATTCGGAGAAATATC
AGCAAAAGGTAGCAGCTGCAATATACCGTGGTATATTACGTTATTTCACGGAAAAAGGAAATCCTCCAGAATAA

Upstream 100 bases:

>100_bases
AGGTGAAAGAAGATATCGATTAAAGCTTCATTAAGACAAGCATAAACTTTCCTCTTGTCCCATATAAGTAATTAGAGGGA
CTAGCTGGAGGAGGAAAGGT

Downstream 100 bases:

>100_bases
GGAGGATTTTTTTGAGCTTCTCTCGTTAACGAAGTTTTCGGAAAATATGTTATACTGGAAATGTAAACGCATTTATTTCA
AGGATAAGCAGAGATAAATT

Product: germination-specific N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: Autolysin; Cell wall hydrolase [H]

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MKRIRIISFALAAVVLFFLVKQEFQITKSWRAWNLPLSGKVIVLDAGHGGPDGGAVGGKDIIEKDITLEITKKVQDYLQE
QGALVILTREGDYDLANKDTKSYSRRKAEDLKKRVEIINKPDVDFFASIHLNALTSSGSKGAQTFYYRSSIENERAAKFI
QAELRTSLENTNRSAKTISHVYLLKYSKTPGALIEAGFLSNVNERYMLNSEKYQQKVAAAIYRGILRYFTEKGNPPE

Sequences:

>Translated_237_residues
MKRIRIISFALAAVVLFFLVKQEFQITKSWRAWNLPLSGKVIVLDAGHGGPDGGAVGGKDIIEKDITLEITKKVQDYLQE
QGALVILTREGDYDLANKDTKSYSRRKAEDLKKRVEIINKPDVDFFASIHLNALTSSGSKGAQTFYYRSSIENERAAKFI
QAELRTSLENTNRSAKTISHVYLLKYSKTPGALIEAGFLSNVNERYMLNSEKYQQKVAAAIYRGILRYFTEKGNPPE
>Mature_237_residues
MKRIRIISFALAAVVLFFLVKQEFQITKSWRAWNLPLSGKVIVLDAGHGGPDGGAVGGKDIIEKDITLEITKKVQDYLQE
QGALVILTREGDYDLANKDTKSYSRRKAEDLKKRVEIINKPDVDFFASIHLNALTSSGSKGAQTFYYRSSIENERAAKFI
QAELRTSLENTNRSAKTISHVYLLKYSKTPGALIEAGFLSNVNERYMLNSEKYQQKVAAAIYRGILRYFTEKGNPPE

Specific function: Unknown

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788776, Length=234, Percent_Identity=29.9145299145299, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI1790611, Length=238, Percent_Identity=25.6302521008403, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI87082163, Length=235, Percent_Identity=26.8085106382979, Blast_Score=78, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508
- InterPro:   IPR014234 [H]

Pfam domain/function: PF01520 Amidase_3 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 26688; Mature: 26688

Theoretical pI: Translated: 10.01; Mature: 10.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRIRIISFALAAVVLFFLVKQEFQITKSWRAWNLPLSGKVIVLDAGHGGPDGGAVGGKD
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCEEEEEECCCCCCCCCCCCCHH
IIEKDITLEITKKVQDYLQEQGALVILTREGDYDLANKDTKSYSRRKAEDLKKRVEIINK
HHHCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
PDVDFFASIHLNALTSSGSKGAQTFYYRSSIENERAAKFIQAELRTSLENTNRSAKTISH
CCCCEEHHEEEEHHHCCCCCCCEEEEEHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHE
VYLLKYSKTPGALIEAGFLSNVNERYMLNSEKYQQKVAAAIYRGILRYFTEKGNPPE
EEEEEECCCCCHHEEHHHHCCCCHHEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKRIRIISFALAAVVLFFLVKQEFQITKSWRAWNLPLSGKVIVLDAGHGGPDGGAVGGKD
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCEEEEEECCCCCCCCCCCCCHH
IIEKDITLEITKKVQDYLQEQGALVILTREGDYDLANKDTKSYSRRKAEDLKKRVEIINK
HHHCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
PDVDFFASIHLNALTSSGSKGAQTFYYRSSIENERAAKFIQAELRTSLENTNRSAKTISH
CCCCEEHHEEEEHHHCCCCCCCEEEEEHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHE
VYLLKYSKTPGALIEAGFLSNVNERYMLNSEKYQQKVAAAIYRGILRYFTEKGNPPE
EEEEEECCCCCHHEEHHHHCCCCHHEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7559346; 8969501; 9384377 [H]