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The map label for this gene is insK [H]

Identifier: 218231138

GI number: 218231138

Start: 2919682

End: 2920401

Strand: Reverse

Name: insK [H]

Synonym: BCB4264_A3041

Alternate gene names: 218231138

Gene position: 2920401-2919682 (Counterclockwise)

Preceding gene: 218235925

Following gene: 218231247

Centisome position: 53.89

GC content: 32.08

Gene sequence:

>720_bases
ATGTCTAAAATAGTAGAATGCGAACGAGATCCAGATATAAATGGAAGCTATGGTTACCGAAGAATTTGTATATGGCTTAA
AAAGTATTATGGTTTACAGATAAATCACAAAAGAGTATATCGTTTAATGAGGAAAATGGGGATACAAGCTAAGATTCGAA
AGAAAAAGTGGAGGCATTTTGGTTTAAAAGAACAATGCGTCGTGTCGGAAAATCTGTTAAACAGAGAGTTTTCAACTACA
AGACCAAATGAAAAATGGGTTACAGATATAACCTATCTTACCTTTAACAATAAGCGCATATATCTTTCCGTGATACTAGA
CCTTTACAATAACGAAGTTGTGGCCTACCAAATGAGCGAATACAACAATTTAAAATTAGTTACAGACACAGTCAATAAAG
CCTTAAGAAAAAGAAAAGTCCACGAAACAGTATTACATAGCGACCGAGGTTATCAGTATACGTCAAAAAAATATAACCAA
TTACTTAAAAAGCACAAAATAACTGTGAGTATGTCTAGGAAGGGAAATTGTTACGATAATGCATGTATAGAGAGTTTTTT
TAGTCATTTTAAGGCAGAATGCTTTTATAGACATACGTTTCATGAAAAAGCAGAAGTTTTTAAGGCTACTAAAAGATATA
TGGGCTATTACAATAACAAAAGATTTCAAAAAAGATTAAACAACCTTAGTCCTATTGAATTTGGAACTAAGGCTGCTTAA

Upstream 100 bases:

>100_bases
ATTGCTTTGTGAAATTGCAGGTGTTTCAAGAAGTGGATATTATAAGTGGGAAAAAAGAATCAACGATCCCTCTGAAAAGA
TGAAAGAGGATCAATTTATC

Downstream 100 bases:

>100_bases
TGAAAAGACACCTAATCTATTACTGTCTACTTGACAGGGGTAAGACCATATGGCTGAAGTCATCTTTTTTGTATAAGTAG
AATCCATTTTGGACTCAGTT

Product: transposase InsF for insertion sequence IS3A/B/C/D/E/fA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 239; Mature: 238

Protein sequence:

>239_residues
MSKIVECERDPDINGSYGYRRICIWLKKYYGLQINHKRVYRLMRKMGIQAKIRKKKWRHFGLKEQCVVSENLLNREFSTT
RPNEKWVTDITYLTFNNKRIYLSVILDLYNNEVVAYQMSEYNNLKLVTDTVNKALRKRKVHETVLHSDRGYQYTSKKYNQ
LLKKHKITVSMSRKGNCYDNACIESFFSHFKAECFYRHTFHEKAEVFKATKRYMGYYNNKRFQKRLNNLSPIEFGTKAA

Sequences:

>Translated_239_residues
MSKIVECERDPDINGSYGYRRICIWLKKYYGLQINHKRVYRLMRKMGIQAKIRKKKWRHFGLKEQCVVSENLLNREFSTT
RPNEKWVTDITYLTFNNKRIYLSVILDLYNNEVVAYQMSEYNNLKLVTDTVNKALRKRKVHETVLHSDRGYQYTSKKYNQ
LLKKHKITVSMSRKGNCYDNACIESFFSHFKAECFYRHTFHEKAEVFKATKRYMGYYNNKRFQKRLNNLSPIEFGTKAA
>Mature_238_residues
SKIVECERDPDINGSYGYRRICIWLKKYYGLQINHKRVYRLMRKMGIQAKIRKKKWRHFGLKEQCVVSENLLNREFSTTR
PNEKWVTDITYLTFNNKRIYLSVILDLYNNEVVAYQMSEYNNLKLVTDTVNKALRKRKVHETVLHSDRGYQYTSKKYNQL
LKKHKITVSMSRKGNCYDNACIESFFSHFKAECFYRHTFHEKAEVFKATKRYMGYYNNKRFQKRLNNLSPIEFGTKAA

Specific function: Involved in the transposition of the insertion sequence IS150 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=213, Percent_Identity=30.9859154929577, Blast_Score=104, Evalue=8e-23,
Organism=Escherichia coli, GI1789981, Length=221, Percent_Identity=45.2488687782805, Blast_Score=205, Evalue=3e-54,
Organism=Escherichia coli, GI1788405, Length=216, Percent_Identity=38.4259259259259, Blast_Score=144, Evalue=7e-36,
Organism=Escherichia coli, GI1787263, Length=216, Percent_Identity=38.4259259259259, Blast_Score=144, Evalue=7e-36,
Organism=Escherichia coli, GI1786753, Length=216, Percent_Identity=38.4259259259259, Blast_Score=144, Evalue=7e-36,
Organism=Escherichia coli, GI1786570, Length=216, Percent_Identity=38.4259259259259, Blast_Score=144, Evalue=7e-36,
Organism=Escherichia coli, GI1786490, Length=216, Percent_Identity=38.4259259259259, Blast_Score=144, Evalue=7e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 28786; Mature: 28654

Theoretical pI: Translated: 10.39; Mature: 10.39

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKIVECERDPDINGSYGYRRICIWLKKYYGLQINHKRVYRLMRKMGIQAKIRKKKWRHF
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCEECHHHHHHHHHHHCCHHHHHHHHHHHC
GLKEQCVVSENLLNREFSTTRPNEKWVTDITYLTFNNKRIYLSVILDLYNNEVVAYQMSE
CCCHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEECCCEEEEEEEEHHHCCCEEEEEECC
YNNLKLVTDTVNKALRKRKVHETVLHSDRGYQYTSKKYNQLLKKHKITVSMSRKGNCYDN
CCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCEEEEECCCCCCCHH
ACIESFFSHFKAECFYRHTFHEKAEVFKATKRYMGYYNNKRFQKRLNNLSPIEFGTKAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SKIVECERDPDINGSYGYRRICIWLKKYYGLQINHKRVYRLMRKMGIQAKIRKKKWRHF
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCEECHHHHHHHHHHHCCHHHHHHHHHHHC
GLKEQCVVSENLLNREFSTTRPNEKWVTDITYLTFNNKRIYLSVILDLYNNEVVAYQMSE
CCCHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEECCCEEEEEEEEHHHCCCEEEEEECC
YNNLKLVTDTVNKALRKRKVHETVLHSDRGYQYTSKKYNQLLKKHKITVSMSRKGNCYDN
CCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCEEEEECCCCCCCHH
ACIESFFSHFKAECFYRHTFHEKAEVFKATKRYMGYYNNKRFQKRLNNLSPIEFGTKAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2841644; 2541046; 8041620; 9278503 [H]