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The map label for this gene is ansA2 [H]

Identifier: 218231106

GI number: 218231106

Start: 2987177

End: 2988181

Strand: Reverse

Name: ansA2 [H]

Synonym: BCB4264_A3116

Alternate gene names: 218231106

Gene position: 2988181-2987177 (Counterclockwise)

Preceding gene: 218230843

Following gene: 218233268

Centisome position: 55.14

GC content: 35.52

Gene sequence:

>1005_bases
ATGAAGAAAATTTTATTACTTGCAACTGGTGGGACAATTGCATCTGTGGAGGGGAATGAGGGGCTTGTTCCAGGATTGTC
CGCTGAGGAATTATTGAATTATTTTTCGAAGTCTTCTCGAAACTTAGAAATAGATTGCAAAATCTTAATGAATATTGATA
GTACCAATATGCAACCTGAGCACTGGAAAGAAATAGCTAATGCTGTTTTTAATCACTATGATGACTATGACGGGTTTGTG
ATTACACATGGAACGGATACGTTAGCTTATACATCTTCTGCGTTATCTTATATGCTGCAAGGTTTAAGAAAACCAGTTGT
TTTAACAGGTTCACAAGTACCTATTAGTTTTAAAAAGACAGACGCAAAGAAAAATGTAGCTGATGCTCTTAGGTTTGCCT
GTGAGGATGTTGGCGGTGTGTTTATTGTTTTTGATAGTAGGGTGATTATTGGGACGAGAGCAGTTAAAATGCGAACAAAA
AGTTATGATGCATTTGAAAGTGTGAATTATCCATATGTTGCTGAGGTAAATGAAGATGAAGTGAAATATCATTGGAAGCC
AACTTCCTCACATAGTGAACTTTCAATAAATACTGATTTATGTACGGATGTTTTTCTTATGAAATTGTATCCAGGTACAA
AGCCTGAGGTATTTGATTGTTTAAAAGATTTGTATAAAGGGATTATTATTGAAAGTTTTGGAAACGGTGGATTACCTTTT
GAAGGAAGGAACTTGCTTTCGAAAATTCAAGAGTTGACTGAAATGGGGATTGCTGTAGTGATTACTACTCAATGCCTGGA
AGAAGGAGAAGACATACTTCTCTATGAAGTAGGACGGAAAGTGGCGCAACATCAAGTAATATTGTCTGGAGATATGAATA
CGGAAGCCATCATAGCAAAATTGATGTGGGCGCTTGGGAAAACTAATAAATTAGAAGAGATTAAAAAAATTATTGAAGAG
CCTTTAGCCTACGATTTAACAATAAAATCTGATAAGGATTGGTAA

Upstream 100 bases:

>100_bases
TACAAAATCTTGATTAATTCAAATTAAGTTCTATAATGATAAATATAAATACGCATTACGCGAACGATATATCGTTGTTT
ATTTTATGGAGGGGTTGTGT

Downstream 100 bases:

>100_bases
TGACTTCATATGATTTATTTAGGTTTTACATTTAATATGGGGGAGAAATTAAAATGGCAATGACAAACAAGAACGTGCGA
GTGGAGAATGATTTTTTGGG

Product: L-asparaginase

Products: NA

Alternate protein names: L-ASNase 1; L-asparagine amidohydrolase 1 [H]

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MKKILLLATGGTIASVEGNEGLVPGLSAEELLNYFSKSSRNLEIDCKILMNIDSTNMQPEHWKEIANAVFNHYDDYDGFV
ITHGTDTLAYTSSALSYMLQGLRKPVVLTGSQVPISFKKTDAKKNVADALRFACEDVGGVFIVFDSRVIIGTRAVKMRTK
SYDAFESVNYPYVAEVNEDEVKYHWKPTSSHSELSINTDLCTDVFLMKLYPGTKPEVFDCLKDLYKGIIIESFGNGGLPF
EGRNLLSKIQELTEMGIAVVITTQCLEEGEDILLYEVGRKVAQHQVILSGDMNTEAIIAKLMWALGKTNKLEEIKKIIEE
PLAYDLTIKSDKDW

Sequences:

>Translated_334_residues
MKKILLLATGGTIASVEGNEGLVPGLSAEELLNYFSKSSRNLEIDCKILMNIDSTNMQPEHWKEIANAVFNHYDDYDGFV
ITHGTDTLAYTSSALSYMLQGLRKPVVLTGSQVPISFKKTDAKKNVADALRFACEDVGGVFIVFDSRVIIGTRAVKMRTK
SYDAFESVNYPYVAEVNEDEVKYHWKPTSSHSELSINTDLCTDVFLMKLYPGTKPEVFDCLKDLYKGIIIESFGNGGLPF
EGRNLLSKIQELTEMGIAVVITTQCLEEGEDILLYEVGRKVAQHQVILSGDMNTEAIIAKLMWALGKTNKLEEIKKIIEE
PLAYDLTIKSDKDW
>Mature_334_residues
MKKILLLATGGTIASVEGNEGLVPGLSAEELLNYFSKSSRNLEIDCKILMNIDSTNMQPEHWKEIANAVFNHYDDYDGFV
ITHGTDTLAYTSSALSYMLQGLRKPVVLTGSQVPISFKKTDAKKNVADALRFACEDVGGVFIVFDSRVIIGTRAVKMRTK
SYDAFESVNYPYVAEVNEDEVKYHWKPTSSHSELSINTDLCTDVFLMKLYPGTKPEVFDCLKDLYKGIIIESFGNGGLPF
EGRNLLSKIQELTEMGIAVVITTQCLEEGEDILLYEVGRKVAQHQVILSGDMNTEAIIAKLMWALGKTNKLEEIKKIIEE
PLAYDLTIKSDKDW

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=360, Percent_Identity=31.3888888888889, Blast_Score=150, Evalue=2e-36,
Organism=Escherichia coli, GI1788065, Length=336, Percent_Identity=34.8214285714286, Blast_Score=183, Evalue=1e-47,
Organism=Escherichia coli, GI1789327, Length=304, Percent_Identity=25.6578947368421, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI193207372, Length=361, Percent_Identity=31.8559556786704, Blast_Score=162, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17558278, Length=365, Percent_Identity=31.5068493150685, Blast_Score=162, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17558280, Length=365, Percent_Identity=31.5068493150685, Blast_Score=162, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI193207374, Length=365, Percent_Identity=30.958904109589, Blast_Score=162, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6320527, Length=332, Percent_Identity=25, Blast_Score=90, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6323189, Length=279, Percent_Identity=26.5232974910394, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6323187, Length=279, Percent_Identity=26.5232974910394, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6323186, Length=279, Percent_Identity=26.5232974910394, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6323184, Length=279, Percent_Identity=26.5232974910394, Blast_Score=84, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24645530, Length=266, Percent_Identity=36.8421052631579, Blast_Score=165, Evalue=3e-41,
Organism=Drosophila melanogaster, GI24639609, Length=282, Percent_Identity=34.3971631205674, Blast_Score=163, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006033
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 37304; Mature: 37304

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKILLLATGGTIASVEGNEGLVPGLSAEELLNYFSKSSRNLEIDCKILMNIDSTNMQPE
CCEEEEEEECCEEEEECCCCCCCCCCCHHHHHHHHHCCCCCEEEEEEEEEEECCCCCCHH
HWKEIANAVFNHYDDYDGFVITHGTDTLAYTSSALSYMLQGLRKPVVLTGSQVPISFKKT
HHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEECC
DAKKNVADALRFACEDVGGVFIVFDSRVIIGTRAVKMRTKSYDAFESVNYPYVAEVNEDE
CCHHHHHHHHHHHHHHCCCEEEEECCEEEEEEEEEEEECCCCHHHHCCCCCEEEECCCCC
VKYHWKPTSSHSELSINTDLCTDVFLMKLYPGTKPEVFDCLKDLYKGIIIESFGNGGLPF
EEEEECCCCCCCEEEECHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCEEEEECCCCCCCC
EGRNLLSKIQELTEMGIAVVITTQCLEEGEDILLYEVGRKVAQHQVILSGDMNTEAIIAK
CCHHHHHHHHHHHHCCEEEEEEEHHHHCCCCEEEEHHHHHHHHCEEEEECCCCHHHHHHH
LMWALGKTNKLEEIKKIIEEPLAYDLTIKSDKDW
HHHHHCCCCCHHHHHHHHHCCCEEEEEECCCCCC
>Mature Secondary Structure
MKKILLLATGGTIASVEGNEGLVPGLSAEELLNYFSKSSRNLEIDCKILMNIDSTNMQPE
CCEEEEEEECCEEEEECCCCCCCCCCCHHHHHHHHHCCCCCEEEEEEEEEEECCCCCCHH
HWKEIANAVFNHYDDYDGFVITHGTDTLAYTSSALSYMLQGLRKPVVLTGSQVPISFKKT
HHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEECC
DAKKNVADALRFACEDVGGVFIVFDSRVIIGTRAVKMRTKSYDAFESVNYPYVAEVNEDE
CCHHHHHHHHHHHHHHCCCEEEEECCEEEEEEEEEEEECCCCHHHHCCCCCEEEECCCCC
VKYHWKPTSSHSELSINTDLCTDVFLMKLYPGTKPEVFDCLKDLYKGIIIESFGNGGLPF
EEEEECCCCCCCEEEECHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCEEEEECCCCCCCC
EGRNLLSKIQELTEMGIAVVITTQCLEEGEDILLYEVGRKVAQHQVILSGDMNTEAIIAK
CCHHHHHHHHHHHHCCEEEEEEEHHHHCCCCEEEEHHHHHHHHCEEEEECCCCHHHHHHH
LMWALGKTNKLEEIKKIIEEPLAYDLTIKSDKDW
HHHHHCCCCCHHHHHHHHHCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1711029; 8969508; 9384377; 8478318 [H]