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The map label for this gene is dacB [H]

Identifier: 218231015

GI number: 218231015

Start: 1286199

End: 1287485

Strand: Direct

Name: dacB [H]

Synonym: BCB4264_A1328

Alternate gene names: 218231015

Gene position: 1286199-1287485 (Clockwise)

Preceding gene: 218234119

Following gene: 218235299

Centisome position: 23.73

GC content: 36.13

Gene sequence:

>1287_bases
ATGGTAAATTTTAAGAAAATTTTGGTTTTTATTACAGTTATTTGTTTGTTTTTGTTGACACCTATGACATTGCGTGCTGA
GACGAATATTGGAGTAAATCCAGAACAAGTTGCACCACCACCTGCAGAAGGACCGAATGTTTTTAGTCAGTTTGCAACGA
CAATTGATGCAAAAACAGGAGATGTTTTGTATGACAAAAACGCATATCACCGTGCATATCCTGCTAGTACGACGAAGGTG
TTAACAGCGATTTTACTCATGGAGCATACAAAGCCAGAGGATCAATTTACATTTTCACAATTAGCATTAGATCAAGAGAA
GAGTAATTATCAAATTGAGTTTCAACCTGGAGAGACAATTAATAGAAATACTGCGCTTATGATTTTAATGGTGCTGAGTG
CGAATGATGTGTCGTATGCGATTGCGGAGCGGATTGGCGGGAGTGTTGAGAATTTCGCTAATATGATGAATGAGAAAGCG
AAGCAATTAGGGGCGAAAGATAGCCACTTTGTAACACCGAATGGATTACATGATCCGAACCATTATACGACGCCGTATGA
TATGGCTATGATTACGAAGGGTGTGCAAAAGTATCCTGAAATTTTACAAGCGATGAATACGAAAAGAACGACAGTTACGA
CGTCTAGGCAGACGGTGTCTATTTTTAATAAATCTAATTTTTTTGAAAATCCATATAGCATTGGCGGTAAAACAGGGTTT
ACGAATGAAGCGCGTAATACACTTGTTTTATTAAATGAGAAAGATGGAAATCGTATTATAAGCGTAGTAATGGCTTCTCA
AAGACCTGAAATTTATGAAGATTTGAAGCAGATGGCGGACTATTCTTTCGGTCAATTTACGAAGCAAATGGTGCTTGATA
AACATAATTGGCATCAAAAAACAACATATTTAAATAAAGATGTTAATTCTGAGTTGGAACAAAGTGCAGAGCTTATGCTT
AAGAAAGATGAAGGGAAAAATGTGAAAACTATTTTCCGGGCGGCTTCATTAGATAAAGAATCTTTATATCATAAAGGAAT
ACATCGTGGTGAGGTAGTTGGTGCAGTTGATATTACGAAAAATAATCAAACAATTGCGACGGTAAATGTTCTTTCAACAG
AAGATGTCACTTTTGCGATGCCTAGAAAAGATACAACTATGCCTGAAGTAAAAGACTCAAATATGAAAGTAATAAGTGTT
GGGATAGGTGCGATTGTATTATTTGGAGCAATTTTATATGTAGTGTTGCGCCGAAATACAAAAGGAATGAAATCAGAAGG
TAAATAA

Upstream 100 bases:

>100_bases
AGTAAAAAATAGTGAAAATTCTTCCAGTTTAATACAAATTACATATGATTGGTAGATTTATTATTTTGTTAGAGGAGTAA
ATAGAAAAAGGGAGAGTCAT

Downstream 100 bases:

>100_bases
ATTACTAATAAAAAGACTGTGTAAAATTTTGGCACAGTCTTTTTTATATATCTTAAATAACTAATAATTGTAAATTTTCT
TATTGCAATAACTTGAAGTT

Product: D-alanyl-D-alanine carboxypeptidase family protein

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5*; PBP-5* [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MVNFKKILVFITVICLFLLTPMTLRAETNIGVNPEQVAPPPAEGPNVFSQFATTIDAKTGDVLYDKNAYHRAYPASTTKV
LTAILLMEHTKPEDQFTFSQLALDQEKSNYQIEFQPGETINRNTALMILMVLSANDVSYAIAERIGGSVENFANMMNEKA
KQLGAKDSHFVTPNGLHDPNHYTTPYDMAMITKGVQKYPEILQAMNTKRTTVTTSRQTVSIFNKSNFFENPYSIGGKTGF
TNEARNTLVLLNEKDGNRIISVVMASQRPEIYEDLKQMADYSFGQFTKQMVLDKHNWHQKTTYLNKDVNSELEQSAELML
KKDEGKNVKTIFRAASLDKESLYHKGIHRGEVVGAVDITKNNQTIATVNVLSTEDVTFAMPRKDTTMPEVKDSNMKVISV
GIGAIVLFGAILYVVLRRNTKGMKSEGK

Sequences:

>Translated_428_residues
MVNFKKILVFITVICLFLLTPMTLRAETNIGVNPEQVAPPPAEGPNVFSQFATTIDAKTGDVLYDKNAYHRAYPASTTKV
LTAILLMEHTKPEDQFTFSQLALDQEKSNYQIEFQPGETINRNTALMILMVLSANDVSYAIAERIGGSVENFANMMNEKA
KQLGAKDSHFVTPNGLHDPNHYTTPYDMAMITKGVQKYPEILQAMNTKRTTVTTSRQTVSIFNKSNFFENPYSIGGKTGF
TNEARNTLVLLNEKDGNRIISVVMASQRPEIYEDLKQMADYSFGQFTKQMVLDKHNWHQKTTYLNKDVNSELEQSAELML
KKDEGKNVKTIFRAASLDKESLYHKGIHRGEVVGAVDITKNNQTIATVNVLSTEDVTFAMPRKDTTMPEVKDSNMKVISV
GIGAIVLFGAILYVVLRRNTKGMKSEGK
>Mature_428_residues
MVNFKKILVFITVICLFLLTPMTLRAETNIGVNPEQVAPPPAEGPNVFSQFATTIDAKTGDVLYDKNAYHRAYPASTTKV
LTAILLMEHTKPEDQFTFSQLALDQEKSNYQIEFQPGETINRNTALMILMVLSANDVSYAIAERIGGSVENFANMMNEKA
KQLGAKDSHFVTPNGLHDPNHYTTPYDMAMITKGVQKYPEILQAMNTKRTTVTTSRQTVSIFNKSNFFENPYSIGGKTGF
TNEARNTLVLLNEKDGNRIISVVMASQRPEIYEDLKQMADYSFGQFTKQMVLDKHNWHQKTTYLNKDVNSELEQSAELML
KKDEGKNVKTIFRAASLDKESLYHKGIHRGEVVGAVDITKNNQTIATVNVLSTEDVTFAMPRKDTTMPEVKDSNMKVISV
GIGAIVLFGAILYVVLRRNTKGMKSEGK

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors. Required specifically for the synthesis of the spore form of peptidoglycan (cortex) [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Forespore outer membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=375, Percent_Identity=25.3333333333333, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI87082022, Length=384, Percent_Identity=23.4375, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1786851, Length=337, Percent_Identity=24.6290801186944, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI87082054, Length=224, Percent_Identity=27.2321428571429, Blast_Score=72, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 47907; Mature: 47907

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNFKKILVFITVICLFLLTPMTLRAETNIGVNPEQVAPPPAEGPNVFSQFATTIDAKTG
CCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHCCCCCCCCCHHHHHHHHHHCCCCC
DVLYDKNAYHRAYPASTTKVLTAILLMEHTKPEDQFTFSQLALDQEKSNYQIEFQPGETI
CEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCC
NRNTALMILMVLSANDVSYAIAERIGGSVENFANMMNEKAKQLGAKDSHFVTPNGLHDPN
CCCCEEEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCC
HYTTPYDMAMITKGVQKYPEILQAMNTKRTTVTTSRQTVSIFNKSNFFENPYSIGGKTGF
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEECCCCCCCCCCCCCCCCCC
TNEARNTLVLLNEKDGNRIISVVMASQRPEIYEDLKQMADYSFGQFTKQMVLDKHNWHQK
CCCCCCEEEEEECCCCCEEEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHH
TTYLNKDVNSELEQSAELMLKKDEGKNVKTIFRAASLDKESLYHKGIHRGEVVGAVDITK
HHHHCCCHHHHHHHHHHHEEECCCCCCHHHHHHHHCCCHHHHHHCCCCCCCEEEEEEEEC
NNQTIATVNVLSTEDVTFAMPRKDTTMPEVKDSNMKVISVGIGAIVLFGAILYVVLRRNT
CCCEEEEEEEEECCCEEEECCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCC
KGMKSEGK
CCCCCCCC
>Mature Secondary Structure
MVNFKKILVFITVICLFLLTPMTLRAETNIGVNPEQVAPPPAEGPNVFSQFATTIDAKTG
CCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHCCCCCCCCCHHHHHHHHHHCCCCC
DVLYDKNAYHRAYPASTTKVLTAILLMEHTKPEDQFTFSQLALDQEKSNYQIEFQPGETI
CEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCC
NRNTALMILMVLSANDVSYAIAERIGGSVENFANMMNEKAKQLGAKDSHFVTPNGLHDPN
CCCCEEEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCC
HYTTPYDMAMITKGVQKYPEILQAMNTKRTTVTTSRQTVSIFNKSNFFENPYSIGGKTGF
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEECCCCCCCCCCCCCCCCCC
TNEARNTLVLLNEKDGNRIISVVMASQRPEIYEDLKQMADYSFGQFTKQMVLDKHNWHQK
CCCCCCEEEEEECCCCCEEEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHH
TTYLNKDVNSELEQSAELMLKKDEGKNVKTIFRAASLDKESLYHKGIHRGEVVGAVDITK
HHHHCCCHHHHHHHHHHHEEECCCCCCHHHHHHHHCCCHHHHHHCCCCCCCEEEEEEEEC
NNQTIATVNVLSTEDVTFAMPRKDTTMPEVKDSNMKVISVGIGAIVLFGAILYVVLRRNT
CCCEEEEEEEEECCCEEEECCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCC
KGMKSEGK
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1548223; 7934829; 9384377 [H]