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The map label for this gene is yfkF [H]

Identifier: 218230855

GI number: 218230855

Start: 3183763

End: 3184959

Strand: Reverse

Name: yfkF [H]

Synonym: BCB4264_A3301

Alternate gene names: 218230855

Gene position: 3184959-3183763 (Counterclockwise)

Preceding gene: 218231243

Following gene: 218231253

Centisome position: 58.77

GC content: 37.68

Gene sequence:

>1197_bases
ATGTCAATGCGTTTTACTTTTTGGATTATGGTTGGGATTGTAGCAATCTCGGGTTTGTCACAAGGAATGCTCTTACCTGC
CATTGCAATGATTTTTGAACAAGAAGGGGTTAGTTCAAGTATTAATGGTATTCATGCGACGGCATTGTACATTGGGATAT
TAGTTATTTCGCCGTTTCTTGAAAAACCGATGCAAAGGTTTGGAATGAAGCCGATTATTGTTATAGGTGGGTTTCTCGTT
ATTATTTCATTATTCTTTTTTACACAAACATTTTCATTTTGGGTATGGTTTATCCTTAGATTTCTAGTTGGAGTTGGAGA
TCATATGCTTCATGTCGGAACACAAACATGGATCACGACAACATCAGACCCAAGTAAAATAGGGAGACAAGTATCGATAT
ACGGTGTATTCTTCGGAATTGGTTTTGCCGTTGGTCCGTATTTAGCAAGCACTGTGCAGTACGGTCTTGCAACACCATTT
ATTATATCTACTATACTTTGTCTAATAGGTTGGCTTTTACTACTTCCAACAAAAAATGCATTCCCAGCGCAAGATGAAAG
AGAAGTGAAGAGTGAATCATCATTTTCTCGTTATAAACAAGTTGTTGGATTAGGATGGATTGCACTGCTAGGACCACTTG
CATATGGCGTACTTGAAGCAATGTTAAACAGTAACTTACCAGTATACGCACTTCGTAAAGGGTGGTCTGTTTCAGATGTA
TCCTTCTTATTACCAGCATTTGCAGTTGGGGGTATTATTACACAAATTCCGCTCGGTATATTGAGTGATAAATACGGAAG
GGACCGTATATTAACGTGGACGTTTAGCATAAGTACGGGCATTTTTCTACTTGCCGCCGTATTTGATCAATATTACTGGA
TCGTCTTTGCCTGCATGCTTTTAGCGGGTATGGTTATTGGATCGTGCTTCTCTTTAGGGCTTGGATTTATGACTGATTTA
TTACCGAGACATTTACTACCGGCAGGTAATATATTATCCGGAATCGCTTTTAGTTTAGGAAGTATTATGGGACCTGTATT
AGGCGGCGTATTTATAGAAAAAATACAGTATACAAGCTTTTTTATTGCGGTTATGATTATAATGGGAATTCTCGCAATGT
TATATATGGTCTACATGAAAAATCAATTTGCATCAAGAAAAATAGAAAGTAGGTTAGGGCATGACAAAACAACCTAA

Upstream 100 bases:

>100_bases
TCATTCTTTTAGCTTCTTTTTTATTGCGAATATTCGTACACGCATTTGAAAAAACAGTTTATAATGAAAAGTGGAAGCGT
GAGAAGGAGAGGGACAGAGG

Downstream 100 bases:

>100_bases
TAAAAAGAAATTTGAAGTACTTGAAAATGAAACAATTACAGACTGCTTAGCACGTATGGAACAAGAAGGCTACGCTCCGT
CTCGTCGTATGGAAGAGCCA

Product: major facilitator family transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MSMRFTFWIMVGIVAISGLSQGMLLPAIAMIFEQEGVSSSINGIHATALYIGILVISPFLEKPMQRFGMKPIIVIGGFLV
IISLFFFTQTFSFWVWFILRFLVGVGDHMLHVGTQTWITTTSDPSKIGRQVSIYGVFFGIGFAVGPYLASTVQYGLATPF
IISTILCLIGWLLLLPTKNAFPAQDEREVKSESSFSRYKQVVGLGWIALLGPLAYGVLEAMLNSNLPVYALRKGWSVSDV
SFLLPAFAVGGIITQIPLGILSDKYGRDRILTWTFSISTGIFLLAAVFDQYYWIVFACMLLAGMVIGSCFSLGLGFMTDL
LPRHLLPAGNILSGIAFSLGSIMGPVLGGVFIEKIQYTSFFIAVMIIMGILAMLYMVYMKNQFASRKIESRLGHDKTT

Sequences:

>Translated_398_residues
MSMRFTFWIMVGIVAISGLSQGMLLPAIAMIFEQEGVSSSINGIHATALYIGILVISPFLEKPMQRFGMKPIIVIGGFLV
IISLFFFTQTFSFWVWFILRFLVGVGDHMLHVGTQTWITTTSDPSKIGRQVSIYGVFFGIGFAVGPYLASTVQYGLATPF
IISTILCLIGWLLLLPTKNAFPAQDEREVKSESSFSRYKQVVGLGWIALLGPLAYGVLEAMLNSNLPVYALRKGWSVSDV
SFLLPAFAVGGIITQIPLGILSDKYGRDRILTWTFSISTGIFLLAAVFDQYYWIVFACMLLAGMVIGSCFSLGLGFMTDL
LPRHLLPAGNILSGIAFSLGSIMGPVLGGVFIEKIQYTSFFIAVMIIMGILAMLYMVYMKNQFASRKIESRLGHDKTT
>Mature_397_residues
SMRFTFWIMVGIVAISGLSQGMLLPAIAMIFEQEGVSSSINGIHATALYIGILVISPFLEKPMQRFGMKPIIVIGGFLVI
ISLFFFTQTFSFWVWFILRFLVGVGDHMLHVGTQTWITTTSDPSKIGRQVSIYGVFFGIGFAVGPYLASTVQYGLATPFI
ISTILCLIGWLLLLPTKNAFPAQDEREVKSESSFSRYKQVVGLGWIALLGPLAYGVLEAMLNSNLPVYALRKGWSVSDVS
FLLPAFAVGGIITQIPLGILSDKYGRDRILTWTFSISTGIFLLAAVFDQYYWIVFACMLLAGMVIGSCFSLGLGFMTDLL
PRHLLPAGNILSGIAFSLGSIMGPVLGGVFIEKIQYTSFFIAVMIIMGILAMLYMVYMKNQFASRKIESRLGHDKTT

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily [H]

Homologues:

Organism=Escherichia coli, GI1787126, Length=337, Percent_Identity=22.8486646884273, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43795; Mature: 43664

Theoretical pI: Translated: 9.38; Mature: 9.38

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMRFTFWIMVGIVAISGLSQGMLLPAIAMIFEQEGVSSSINGIHATALYIGILVISPFL
CCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHCHHHHHHHHHHHHHHHHHH
EKPMQRFGMKPIIVIGGFLVIISLFFFTQTFSFWVWFILRFLVGVGDHMLHVGTQTWITT
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCEEEE
TSDPSKIGRQVSIYGVFFGIGFAVGPYLASTVQYGLATPFIISTILCLIGWLLLLPTKNA
CCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCC
FPAQDEREVKSESSFSRYKQVVGLGWIALLGPLAYGVLEAMLNSNLPVYALRKGWSVSDV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHH
SFLLPAFAVGGIITQIPLGILSDKYGRDRILTWTFSISTGIFLLAAVFDQYYWIVFACML
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHH
LAGMVIGSCFSLGLGFMTDLLPRHLLPAGNILSGIAFSLGSIMGPVLGGVFIEKIQYTSF
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FIAVMIIMGILAMLYMVYMKNQFASRKIESRLGHDKTT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SMRFTFWIMVGIVAISGLSQGMLLPAIAMIFEQEGVSSSINGIHATALYIGILVISPFL
CCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHCHHHHHHHHHHHHHHHHHH
EKPMQRFGMKPIIVIGGFLVIISLFFFTQTFSFWVWFILRFLVGVGDHMLHVGTQTWITT
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCEEEE
TSDPSKIGRQVSIYGVFFGIGFAVGPYLASTVQYGLATPFIISTILCLIGWLLLLPTKNA
CCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCC
FPAQDEREVKSESSFSRYKQVVGLGWIALLGPLAYGVLEAMLNSNLPVYALRKGWSVSDV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHH
SFLLPAFAVGGIITQIPLGILSDKYGRDRILTWTFSISTGIFLLAAVFDQYYWIVFACML
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHH
LAGMVIGSCFSLGLGFMTDLLPRHLLPAGNILSGIAFSLGSIMGPVLGGVFIEKIQYTSF
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FIAVMIIMGILAMLYMVYMKNQFASRKIESRLGHDKTT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]