Click here to switch to the map view.

The map label for this gene is ppx [H]

Identifier: 218230831

GI number: 218230831

Start: 4011235

End: 4012809

Strand: Reverse

Name: ppx [H]

Synonym: BCB4264_A4099

Alternate gene names: 218230831

Gene position: 4012809-4011235 (Counterclockwise)

Preceding gene: 218235476

Following gene: 218232962

Centisome position: 74.05

GC content: 32.13

Gene sequence:

>1575_bases
ATGTTTTTGAAATTTGATAATGAAAGAGTGAGAAGTTTGAAAGAAATATTAAAACAACAGTATGCCATTATAGATATTGG
ATCTAATACAATGCGTTTAGTTATTTATGAAAAGCAAAATGGAGGTTTTTATAAAGAGATTGAAAATACGAAAGTTGTTG
CTAGGTTAAGGAATTACTTAATTGATGGTGTATTGAATGAAGAGGGAATAGAAGTATTATTACAGACATTATTTCAATTT
CAAGAAAGTACAAGATTCCATCAGTTGCATCATGTACTTTGTGTTGCAACAGCAACAATTAGACAGTCGGAGAATCAAGA
GGAAATTAAAAAGCTTGTTGAAGGACAAACGGACTTTACTCTTAGAGTATTATCAGAGTATGAGGAAGCGCGTTACGGAT
ACTTGGCGGTTATGAATTCTACATCGTTTTCAGAAGGAATAACGGTAGATATTGGTGGGGGAAGTACGGAAGTTACATAC
TTTAGAAATAGAGAGATTTTAGAGTACCATAGTTTTCCTTTTGGGGCACTTTCTTTAAAGCAACAATTTATTAAAGGTGA
TATTCCTACTAAAGATGAGTTAGAGGGAATGCAACAGTATTTAGAGTTTCAATTTCGAAAGTTACCATGGTTAATCAATA
AGAAATTGCCTCTTATTGCAATTGGTGGTAGTGCTAGGAACTTAGTAAAAATCCATCAAAATTTAATATGTTATCCTATA
GCAGGATTACATTTATACAAGATGAAAGAAGAAGATATTGAAAGTGTAAAAGAGGAATTGGAAAAATTGTCGTTCATAGA
ACTTCAGAAATTGGAAGGATTAGCAAAAGATCGAGCAGATACGATTATTCCAGCGGTTGAAGTATTTCATACACTTGTTA
ATGTTATAGAGGCACCGGCATTTGTATTAAGTAGAAAAGGGTTAAGAGAAGGTGTTTTCTATGAAGAGTTGACAAAAGAT
TTGGGGATTTCATATTATCCGAACGTAGTTGAAGAAAGTTTGCATTTACTATCACATGAATACGAAATGGACATGGAATT
TGTTATTCAACTTATTAAACATGGAAGATTAATTTGCAAACAACTTGAGGAAATAGGGCTTATTTCTATGACAGTAAAAG
ACTGGGAAGTGTTCCATCAAGCTGCGAAAGTATTTAATATAGGCAAATATATAGACGAAGAAGCGAGCCGTTTACATACG
TTTTATTTATTAGCGAATAAGACAATTGATGGCATGATGCATAAAGATCGAGTAAGGCTAGCGCTTATTGCATCGTATAA
ATCAAAAATGTTATTTAAACAACATTTATCTCCATTTGAAGGTTGGTTTGATAAAAGCGAACAAAAAAAAATCCGCCTGT
TAGGAGCTGTTTTACAATTTTCAGCTACTTTAAATGTAAGGCAACGATCGCTTATTGATACCATATCTATAACAGAAAGC
GAAGAGGGATTAACGTTTAAAGTTTTATGTGAACAATCGGCATTAGCAGAAAAAGTACAAGCTGAAAAACAAAAAAAACA
GTTAGAAAGAGTATTAAAAACGAATATTAATTTATTATTTGAACTAAAACATTAG

Upstream 100 bases:

>100_bases
AAATAGGTATTTATTATACGCAAAAAGTGATTGTATATATTTTCAATATTAAAGCAATATTGTTACAAAAAACAACTATT
TTGTATAATAGTAATAGAAT

Downstream 100 bases:

>100_bases
GTTGTACGGATGTCTTTACGAAAAATTTACACTTTGTAATATTGTAATTTGATAAAATGAAATTAACTAAATTATAGTAA
AAAAGTTTCAAGGGGAAGTG

Product: Ppx/GppA phosphatase family protein

Products: NA

Alternate protein names: ExopolyPase; Metaphosphatase [H]

Number of amino acids: Translated: 524; Mature: 524

Protein sequence:

>524_residues
MFLKFDNERVRSLKEILKQQYAIIDIGSNTMRLVIYEKQNGGFYKEIENTKVVARLRNYLIDGVLNEEGIEVLLQTLFQF
QESTRFHQLHHVLCVATATIRQSENQEEIKKLVEGQTDFTLRVLSEYEEARYGYLAVMNSTSFSEGITVDIGGGSTEVTY
FRNREILEYHSFPFGALSLKQQFIKGDIPTKDELEGMQQYLEFQFRKLPWLINKKLPLIAIGGSARNLVKIHQNLICYPI
AGLHLYKMKEEDIESVKEELEKLSFIELQKLEGLAKDRADTIIPAVEVFHTLVNVIEAPAFVLSRKGLREGVFYEELTKD
LGISYYPNVVEESLHLLSHEYEMDMEFVIQLIKHGRLICKQLEEIGLISMTVKDWEVFHQAAKVFNIGKYIDEEASRLHT
FYLLANKTIDGMMHKDRVRLALIASYKSKMLFKQHLSPFEGWFDKSEQKKIRLLGAVLQFSATLNVRQRSLIDTISITES
EEGLTFKVLCEQSALAEKVQAEKQKKQLERVLKTNINLLFELKH

Sequences:

>Translated_524_residues
MFLKFDNERVRSLKEILKQQYAIIDIGSNTMRLVIYEKQNGGFYKEIENTKVVARLRNYLIDGVLNEEGIEVLLQTLFQF
QESTRFHQLHHVLCVATATIRQSENQEEIKKLVEGQTDFTLRVLSEYEEARYGYLAVMNSTSFSEGITVDIGGGSTEVTY
FRNREILEYHSFPFGALSLKQQFIKGDIPTKDELEGMQQYLEFQFRKLPWLINKKLPLIAIGGSARNLVKIHQNLICYPI
AGLHLYKMKEEDIESVKEELEKLSFIELQKLEGLAKDRADTIIPAVEVFHTLVNVIEAPAFVLSRKGLREGVFYEELTKD
LGISYYPNVVEESLHLLSHEYEMDMEFVIQLIKHGRLICKQLEEIGLISMTVKDWEVFHQAAKVFNIGKYIDEEASRLHT
FYLLANKTIDGMMHKDRVRLALIASYKSKMLFKQHLSPFEGWFDKSEQKKIRLLGAVLQFSATLNVRQRSLIDTISITES
EEGLTFKVLCEQSALAEKVQAEKQKKQLERVLKTNINLLFELKH
>Mature_524_residues
MFLKFDNERVRSLKEILKQQYAIIDIGSNTMRLVIYEKQNGGFYKEIENTKVVARLRNYLIDGVLNEEGIEVLLQTLFQF
QESTRFHQLHHVLCVATATIRQSENQEEIKKLVEGQTDFTLRVLSEYEEARYGYLAVMNSTSFSEGITVDIGGGSTEVTY
FRNREILEYHSFPFGALSLKQQFIKGDIPTKDELEGMQQYLEFQFRKLPWLINKKLPLIAIGGSARNLVKIHQNLICYPI
AGLHLYKMKEEDIESVKEELEKLSFIELQKLEGLAKDRADTIIPAVEVFHTLVNVIEAPAFVLSRKGLREGVFYEELTKD
LGISYYPNVVEESLHLLSHEYEMDMEFVIQLIKHGRLICKQLEEIGLISMTVKDWEVFHQAAKVFNIGKYIDEEASRLHT
FYLLANKTIDGMMHKDRVRLALIASYKSKMLFKQHLSPFEGWFDKSEQKKIRLLGAVLQFSATLNVRQRSLIDTISITES
EEGLTFKVLCEQSALAEKVQAEKQKKQLERVLKTNINLLFELKH

Specific function: Degradation of inorganic polyphosphates. Orthophosphate is released progressively from the ends of polyphosphate of circa 500 residues long, while chains of circa 15 residues compete poorly with polyphosphate as substrate [H]

COG id: COG0248

COG function: function code FP; Exopolyphosphatase

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gppA/ppx family [H]

Homologues:

Organism=Escherichia coli, GI1788848, Length=435, Percent_Identity=24.5977011494253, Blast_Score=103, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022371
- InterPro:   IPR003695 [H]

Pfam domain/function: PF02541 Ppx-GppA [H]

EC number: =3.6.1.11 [H]

Molecular weight: Translated: 60680; Mature: 60680

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLKFDNERVRSLKEILKQQYAIIDIGSNTMRLVIYEKQNGGFYKEIENTKVVARLRNYL
CCEECCHHHHHHHHHHHHHCEEEEEECCCEEEEEEEEECCCCCHHHHCCHHHHHHHHHHH
IDGVLNEEGIEVLLQTLFQFQESTRFHQLHHVLCVATATIRQSENQEEIKKLVEGQTDFT
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHH
LRVLSEYEEARYGYLAVMNSTSFSEGITVDIGGGSTEVTYFRNREILEYHSFPFGALSLK
HHHHHHHHHCCCCEEEEEECCCCCCCEEEEECCCCEEEEEECCCCCHHHCCCCCHHHHHH
QQFIKGDIPTKDELEGMQQYLEFQFRKLPWLINKKLPLIAIGGSARNLVKIHQNLICYPI
HHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHCCCCCEEEECCCHHHHHHHHHCEEEEEE
AGLHLYKMKEEDIESVKEELEKLSFIELQKLEGLAKDRADTIIPAVEVFHTLVNVIEAPA
CCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHH
FVLSRKGLREGVFYEELTKDLGISYYPNVVEESLHLLSHEYEMDMEFVIQLIKHGRLICK
HHHHHCCHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHH
QLEEIGLISMTVKDWEVFHQAAKVFNIGKYIDEEASRLHTFYLLANKTIDGMMHKDRVRL
HHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHH
ALIASYKSKMLFKQHLSPFEGWFDKSEQKKIRLLGAVLQFSATLNVRQRSLIDTISITES
HHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCC
EEGLTFKVLCEQSALAEKVQAEKQKKQLERVLKTNINLLFELKH
CCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECC
>Mature Secondary Structure
MFLKFDNERVRSLKEILKQQYAIIDIGSNTMRLVIYEKQNGGFYKEIENTKVVARLRNYL
CCEECCHHHHHHHHHHHHHCEEEEEECCCEEEEEEEEECCCCCHHHHCCHHHHHHHHHHH
IDGVLNEEGIEVLLQTLFQFQESTRFHQLHHVLCVATATIRQSENQEEIKKLVEGQTDFT
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHH
LRVLSEYEEARYGYLAVMNSTSFSEGITVDIGGGSTEVTYFRNREILEYHSFPFGALSLK
HHHHHHHHHCCCCEEEEEECCCCCCCEEEEECCCCEEEEEECCCCCHHHCCCCCHHHHHH
QQFIKGDIPTKDELEGMQQYLEFQFRKLPWLINKKLPLIAIGGSARNLVKIHQNLICYPI
HHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHCCCCCEEEECCCHHHHHHHHHCEEEEEE
AGLHLYKMKEEDIESVKEELEKLSFIELQKLEGLAKDRADTIIPAVEVFHTLVNVIEAPA
CCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHH
FVLSRKGLREGVFYEELTKDLGISYYPNVVEESLHLLSHEYEMDMEFVIQLIKHGRLICK
HHHHHCCHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHH
QLEEIGLISMTVKDWEVFHQAAKVFNIGKYIDEEASRLHTFYLLANKTIDGMMHKDRVRL
HHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHH
ALIASYKSKMLFKQHLSPFEGWFDKSEQKKIRLLGAVLQFSATLNVRQRSLIDTISITES
HHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCC
EEGLTFKVLCEQSALAEKVQAEKQKKQLERVLKTNINLLFELKH
CCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]