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The map label for this gene is gyrB [H]

Identifier: 21675071

GI number: 21675071

Start: 2127056

End: 2128999

Strand: Direct

Name: gyrB [H]

Synonym: CT2263

Alternate gene names: 21675071

Gene position: 2127056-2128999 (Clockwise)

Preceding gene: 21675070

Following gene: 21675080

Centisome position: 98.71

GC content: 58.64

Gene sequence:

>1944_bases
ATGCAAGAAACCGATATCCAGACCGCGCAGAACGCTTCAACCGAATATGGCGCCACCAATATTCAGGTGCTCGACGGTAT
CGAGCATGTCCGGAAACGTCCGGCCATGTACATCGGCGACATTCACAGCCGCGGCTTGCACCATCTGGTTTACGAAATCG
TTGACAACGCCATCGACGAAACGCTCGCGGGCTACAACGATTACATTCATGTGGCGATGAATCCGGACGGCTCGGTGACC
GTCACCGACCACGGTCGAGGCATTCCGGTCGATATTCATCCGGTCAAGAAGAAGTCGGCGCTCGAACTGGTCATGACGGT
CATTGGCGCCGGCGGCAAGTTCGACAAGGGGGCCTACAAGGTGTCCGGCGGCTTGCACGGCGTGGGCGCCTCCGTGGTCA
ACGCCCTGTCGGAGTGGTGCGAGGTGGAGGTCTATCGTGACGGCAAGATTTTCCGGCAGACCTACCGGCGCGGCGTTCCG
CAAGGCGGGGTCGAGGAGATCGGAACCACCGATCAGCGCGGCACGAAGGTCACCTTTAAACCCGACCCCGAAATCTTCAA
GATCACCGAGTTCCGCAAGGATATTATTCTCGACCGCATGAGAGAGCTGGCGTTCCTGAACAGCAACCTGCGCATCATCG
TGCAGGACGCCGAGGGCAACGAGGAGATATTCCACTACGAGGGCGGCCTGAAAGAGTTTGTGCGCTTCATCGACTCGAAC
CGTCTGAGCCTGCTCAAGGAGCCGATCTTTTTATCGGGCGAGCGCGATTCGACGATGGTCGAGATCGCTTTGCAGTACAA
CGACTCCTATCAGGAGAACATCTTCAGCTACGTCAACAACATCAACACGCACGAAGGTGGTACGCACGTCACCGGCTTCC
GCAAGGCGCTGACCCGCACCCTCAACGCCTACGCGCAGAAGAACGACCTGCTCAAAAACGTCAAGATCACCCTCACCGGC
GACGACTTCAAGGAGGGCCTCACGGCGGTCATTTCGGTAAAAGTTCCGGAGCCGCAGTTCGAGGGGCAGACCAAGACCAA
GCTCGGCAACTCCGAGACGCAGAGCATCGTCGAGACCATTGTCAACGAGCAGCTCGCCGAGTTCGCCGAGAGCAACCCCG
GCACACTGAAGCTCATCATCGAAAAGGTGAAGAGCGCGGCCATTTCGCGCGAGGCAGCCCGCAAGGCCAAGGAGCTGACA
CGCCGCAAGTCGGTGCTCGAAAGCTCGGGCCTGCCCGGCAAGCTGGCCGACTGCTCGATCAACGATCCCGACCACTGCGA
GCTGTACATCGTCGAGGGCGACTCGGCAGGCGGCAGCGCCAAGCAGGGCCGCGACCGAAGCTTCCAGGCAATCCTGCCGC
TGAAAGGAAAGATTCTGAACGTCGAAAAGGCGCGGCTGCACAAGATGCTGGAAAACGAGGAGATCAAGACGATCATCCTC
GCGCTCGGTACCAGCATTGGCGAGGAGGAGTTCTCGCCCGAAAAGCTGCGCTATGGCAAAATCATCATCATGACTGATGC
CGACGTTGACGGCGCGCACATCCGCACGCTGCTCCTGACCTTCTTCTTCCGCTACATGCGCTCGCTCATCGAGGCGGGCA
AGGTGTTCATCGCCCAGCCGCCACTCTACCTGGTCAAGTCGGGCCGAGAGCAGCAATACGCATGGGATGAGGACGAGCGC
CTCAGTATCGTGGAACAGATGAAGAAGCTCCAGAAAGGCAAGGCCAATGTTTCCATCCAGCGCTACAAAGGTCTCGGTGA
AATGAACCCCGAGCAGCTCTGGAGCACCACGATGGATCCGGCTCACCGTTCCTTGCTTCAGGTCACCGTAGAAAACGCCA
TGGAGGCCGACCAGGTCTTTTCAACCCTGATGGGCGACAAGGTCGAACCCCGCCGAGACTTTATCGAGAAGAATGCCCGT
TATGTCAGACGTTTAGATGTCTGA

Upstream 100 bases:

>100_bases
CGCCTTCATGGTTGGCGACAAGGGCTGAAATAGCATATCAGCGGATTTTTTTTATCTTTGGGATCAAAGTTTTTACTGAT
TCATCACGCACGACGATCCC

Downstream 100 bases:

>100_bases
CATAACCTTCACCACCACCTCTTTTTTCAGCTTTTCAATCCAGGCGTTGAACAGCTCCTGGCTCTTGTGGTTCAGGGCCA
GCTCCTGGATTTGAGCGAAG

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 647; Mature: 647

Protein sequence:

>647_residues
MQETDIQTAQNASTEYGATNIQVLDGIEHVRKRPAMYIGDIHSRGLHHLVYEIVDNAIDETLAGYNDYIHVAMNPDGSVT
VTDHGRGIPVDIHPVKKKSALELVMTVIGAGGKFDKGAYKVSGGLHGVGASVVNALSEWCEVEVYRDGKIFRQTYRRGVP
QGGVEEIGTTDQRGTKVTFKPDPEIFKITEFRKDIILDRMRELAFLNSNLRIIVQDAEGNEEIFHYEGGLKEFVRFIDSN
RLSLLKEPIFLSGERDSTMVEIALQYNDSYQENIFSYVNNINTHEGGTHVTGFRKALTRTLNAYAQKNDLLKNVKITLTG
DDFKEGLTAVISVKVPEPQFEGQTKTKLGNSETQSIVETIVNEQLAEFAESNPGTLKLIIEKVKSAAISREAARKAKELT
RRKSVLESSGLPGKLADCSINDPDHCELYIVEGDSAGGSAKQGRDRSFQAILPLKGKILNVEKARLHKMLENEEIKTIIL
ALGTSIGEEEFSPEKLRYGKIIIMTDADVDGAHIRTLLLTFFFRYMRSLIEAGKVFIAQPPLYLVKSGREQQYAWDEDER
LSIVEQMKKLQKGKANVSIQRYKGLGEMNPEQLWSTTMDPAHRSLLQVTVENAMEADQVFSTLMGDKVEPRRDFIEKNAR
YVRRLDV

Sequences:

>Translated_647_residues
MQETDIQTAQNASTEYGATNIQVLDGIEHVRKRPAMYIGDIHSRGLHHLVYEIVDNAIDETLAGYNDYIHVAMNPDGSVT
VTDHGRGIPVDIHPVKKKSALELVMTVIGAGGKFDKGAYKVSGGLHGVGASVVNALSEWCEVEVYRDGKIFRQTYRRGVP
QGGVEEIGTTDQRGTKVTFKPDPEIFKITEFRKDIILDRMRELAFLNSNLRIIVQDAEGNEEIFHYEGGLKEFVRFIDSN
RLSLLKEPIFLSGERDSTMVEIALQYNDSYQENIFSYVNNINTHEGGTHVTGFRKALTRTLNAYAQKNDLLKNVKITLTG
DDFKEGLTAVISVKVPEPQFEGQTKTKLGNSETQSIVETIVNEQLAEFAESNPGTLKLIIEKVKSAAISREAARKAKELT
RRKSVLESSGLPGKLADCSINDPDHCELYIVEGDSAGGSAKQGRDRSFQAILPLKGKILNVEKARLHKMLENEEIKTIIL
ALGTSIGEEEFSPEKLRYGKIIIMTDADVDGAHIRTLLLTFFFRYMRSLIEAGKVFIAQPPLYLVKSGREQQYAWDEDER
LSIVEQMKKLQKGKANVSIQRYKGLGEMNPEQLWSTTMDPAHRSLLQVTVENAMEADQVFSTLMGDKVEPRRDFIEKNAR
YVRRLDV
>Mature_647_residues
MQETDIQTAQNASTEYGATNIQVLDGIEHVRKRPAMYIGDIHSRGLHHLVYEIVDNAIDETLAGYNDYIHVAMNPDGSVT
VTDHGRGIPVDIHPVKKKSALELVMTVIGAGGKFDKGAYKVSGGLHGVGASVVNALSEWCEVEVYRDGKIFRQTYRRGVP
QGGVEEIGTTDQRGTKVTFKPDPEIFKITEFRKDIILDRMRELAFLNSNLRIIVQDAEGNEEIFHYEGGLKEFVRFIDSN
RLSLLKEPIFLSGERDSTMVEIALQYNDSYQENIFSYVNNINTHEGGTHVTGFRKALTRTLNAYAQKNDLLKNVKITLTG
DDFKEGLTAVISVKVPEPQFEGQTKTKLGNSETQSIVETIVNEQLAEFAESNPGTLKLIIEKVKSAAISREAARKAKELT
RRKSVLESSGLPGKLADCSINDPDHCELYIVEGDSAGGSAKQGRDRSFQAILPLKGKILNVEKARLHKMLENEEIKTIIL
ALGTSIGEEEFSPEKLRYGKIIIMTDADVDGAHIRTLLLTFFFRYMRSLIEAGKVFIAQPPLYLVKSGREQQYAWDEDER
LSIVEQMKKLQKGKANVSIQRYKGLGEMNPEQLWSTTMDPAHRSLLQVTVENAMEADQVFSTLMGDKVEPRRDFIEKNAR
YVRRLDV

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=604, Percent_Identity=27.4834437086093, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI19913406, Length=637, Percent_Identity=26.8445839874411, Blast_Score=148, Evalue=1e-35,
Organism=Escherichia coli, GI48994957, Length=549, Percent_Identity=56.4663023679417, Blast_Score=604, Evalue=1e-174,
Organism=Escherichia coli, GI1789408, Length=630, Percent_Identity=39.8412698412698, Blast_Score=426, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17535065, Length=668, Percent_Identity=25.1497005988024, Blast_Score=151, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI212645845, Length=593, Percent_Identity=26.6441821247892, Blast_Score=144, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI212645657, Length=229, Percent_Identity=26.6375545851528, Blast_Score=73, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6324241, Length=557, Percent_Identity=26.5709156193896, Blast_Score=125, Evalue=2e-29,
Organism=Drosophila melanogaster, GI17136538, Length=678, Percent_Identity=24.7787610619469, Blast_Score=139, Evalue=6e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 72548; Mature: 72548

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQETDIQTAQNASTEYGATNIQVLDGIEHVRKRPAMYIGDIHSRGLHHLVYEIVDNAIDE
CCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHH
TLAGYNDYIHVAMNPDGSVTVTDHGRGIPVDIHPVKKKSALELVMTVIGAGGKFDKGAYK
HHCCCCCEEEEEECCCCCEEEEECCCCCCEECCCCCHHHHHHHHHHHHCCCCCCCCCCEE
VSGGLHGVGASVVNALSEWCEVEVYRDGKIFRQTYRRGVPQGGVEEIGTTDQRGTKVTFK
ECCCCCCHHHHHHHHHHHHHEEEEECCCHHHHHHHHCCCCCCCHHHCCCCCCCCCEEEEC
PDPEIFKITEFRKDIILDRMRELAFLNSNLRIIVQDAEGNEEIFHYEGGLKEFVRFIDSN
CCCCEEEHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEECCCHHHHHHHHCCC
RLSLLKEPIFLSGERDSTMVEIALQYNDSYQENIFSYVNNINTHEGGTHVTGFRKALTRT
CHHHHCCCCEECCCCCCEEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
LNAYAQKNDLLKNVKITLTGDDFKEGLTAVISVKVPEPQFEGQTKTKLGNSETQSIVETI
HHHHHHHHHHHCCEEEEEECCCHHCCCEEEEEEECCCCCCCCCHHHCCCCHHHHHHHHHH
VNEQLAEFAESNPGTLKLIIEKVKSAAISREAARKAKELTRRKSVLESSGLPGKLADCSI
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
NDPDHCELYIVEGDSAGGSAKQGRDRSFQAILPLKGKILNVEKARLHKMLENEEIKTIIL
CCCCCEEEEEEECCCCCCCCCCCCCCCCEEEECCCCEEECHHHHHHHHHHCCCHHHHHHH
ALGTSIGEEEFSPEKLRYGKIIIMTDADVDGAHIRTLLLTFFFRYMRSLIEAGKVFIAQP
HHCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECC
PLYLVKSGREQQYAWDEDERLSIVEQMKKLQKGKANVSIQRYKGLGEMNPEQLWSTTMDP
CHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEHHHCCCCCCCHHHHHHCCCCH
AHRSLLQVTVENAMEADQVFSTLMGDKVEPRRDFIEKNARYVRRLDV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQETDIQTAQNASTEYGATNIQVLDGIEHVRKRPAMYIGDIHSRGLHHLVYEIVDNAIDE
CCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHH
TLAGYNDYIHVAMNPDGSVTVTDHGRGIPVDIHPVKKKSALELVMTVIGAGGKFDKGAYK
HHCCCCCEEEEEECCCCCEEEEECCCCCCEECCCCCHHHHHHHHHHHHCCCCCCCCCCEE
VSGGLHGVGASVVNALSEWCEVEVYRDGKIFRQTYRRGVPQGGVEEIGTTDQRGTKVTFK
ECCCCCCHHHHHHHHHHHHHEEEEECCCHHHHHHHHCCCCCCCHHHCCCCCCCCCEEEEC
PDPEIFKITEFRKDIILDRMRELAFLNSNLRIIVQDAEGNEEIFHYEGGLKEFVRFIDSN
CCCCEEEHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEECCCHHHHHHHHCCC
RLSLLKEPIFLSGERDSTMVEIALQYNDSYQENIFSYVNNINTHEGGTHVTGFRKALTRT
CHHHHCCCCEECCCCCCEEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
LNAYAQKNDLLKNVKITLTGDDFKEGLTAVISVKVPEPQFEGQTKTKLGNSETQSIVETI
HHHHHHHHHHHCCEEEEEECCCHHCCCEEEEEEECCCCCCCCCHHHCCCCHHHHHHHHHH
VNEQLAEFAESNPGTLKLIIEKVKSAAISREAARKAKELTRRKSVLESSGLPGKLADCSI
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
NDPDHCELYIVEGDSAGGSAKQGRDRSFQAILPLKGKILNVEKARLHKMLENEEIKTIIL
CCCCCEEEEEEECCCCCCCCCCCCCCCCEEEECCCCEEECHHHHHHHHHHCCCHHHHHHH
ALGTSIGEEEFSPEKLRYGKIIIMTDADVDGAHIRTLLLTFFFRYMRSLIEAGKVFIAQP
HHCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECC
PLYLVKSGREQQYAWDEDERLSIVEQMKKLQKGKANVSIQRYKGLGEMNPEQLWSTTMDP
CHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEHHHCCCCCCCHHHHHHCCCCH
AHRSLLQVTVENAMEADQVFSTLMGDKVEPRRDFIEKNARYVRRLDV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]