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The map label for this gene is rnhB

Identifier: 21675069

GI number: 21675069

Start: 2125957

End: 2126574

Strand: Direct

Name: rnhB

Synonym: CT2261

Alternate gene names: 21675069

Gene position: 2125957-2126574 (Clockwise)

Preceding gene: 21675065

Following gene: 21675070

Centisome position: 98.65

GC content: 62.14

Gene sequence:

>618_bases
ATGCTTTCAACTGATTTTGAATACCCGCTCTGGGAAAGCCTGTCGCAGGTCTGCGGCATCGACGAGGCTGGCCGCGGGCC
GCTAGCCGGGCCGGTGGTCGCCGCGGCGGTGGTTTTTCCGCGCCACTTCAGGCCGACAGGCATCTTCGCAAAGCTCGACG
ATTCGAAAAAGCTGACCGCCGAACTGCGCGACGAGCTGGCTCTGGCCATCCGCGAGTCCGCCGAAAGCTGGGCGCTCGGC
GTCGTCGATGCGGAGACCATCGACCGGATCAACATTCTGCAGGCAACCATGCTCGCCATGAATCTCGCAGTCGAATCGCT
CGGCTCGACGCCAGAGTTCCTGTTGGTGGACGGCAACCGCTTCAGGCCCGTGCTGCCGATTCCGTACCAGACCATCGTCA
AGGGGGACTCGAAGGTCTTCTCCATCGCCGCCGCCTCTGTGCTGGCCAAAACGCACAGGGACGAGCTAATGACCACCAGC
GCCGCAGAGTACCCAGAGTACGGATTCGAGGTTCATTTTGGCTACCCGACCGCCCGGCACGTCGAGGCAATCGCCCGGCA
CGGTCGCTGCGCCATCCACCGCCAAAGCTTCAAACTTCGGAAACTGGGAGAAAAGTAA

Upstream 100 bases:

>100_bases
CACTTCACGTCTTGGTACGTTGCCCATTCAGCGAACATTTTTGTCTGGTTCCCGGATTTTCGCACCTTGAAAGAGAGTCT
CCCCAACCGTTACGTTTACC

Downstream 100 bases:

>100_bases
AAAACAAGGAACTGCCGTCATGAATGCCCCTCAATGGCTCGGCGCTGAAGGAGAAAAAATCGCCGCAAGACATCTGGCCG
CAAAAGGCTACCGCATCGTT

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 205; Mature: 205

Protein sequence:

>205_residues
MLSTDFEYPLWESLSQVCGIDEAGRGPLAGPVVAAAVVFPRHFRPTGIFAKLDDSKKLTAELRDELALAIRESAESWALG
VVDAETIDRINILQATMLAMNLAVESLGSTPEFLLVDGNRFRPVLPIPYQTIVKGDSKVFSIAAASVLAKTHRDELMTTS
AAEYPEYGFEVHFGYPTARHVEAIARHGRCAIHRQSFKLRKLGEK

Sequences:

>Translated_205_residues
MLSTDFEYPLWESLSQVCGIDEAGRGPLAGPVVAAAVVFPRHFRPTGIFAKLDDSKKLTAELRDELALAIRESAESWALG
VVDAETIDRINILQATMLAMNLAVESLGSTPEFLLVDGNRFRPVLPIPYQTIVKGDSKVFSIAAASVLAKTHRDELMTTS
AAEYPEYGFEVHFGYPTARHVEAIARHGRCAIHRQSFKLRKLGEK
>Mature_205_residues
MLSTDFEYPLWESLSQVCGIDEAGRGPLAGPVVAAAVVFPRHFRPTGIFAKLDDSKKLTAELRDELALAIRESAESWALG
VVDAETIDRINILQATMLAMNLAVESLGSTPEFLLVDGNRFRPVLPIPYQTIVKGDSKVFSIAAASVLAKTHRDELMTTS
AAEYPEYGFEVHFGYPTARHVEAIARHGRCAIHRQSFKLRKLGEK

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Escherichia coli, GI1786380, Length=193, Percent_Identity=49.2227979274611, Blast_Score=168, Evalue=2e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_CHLTE (Q8KAA5)

Other databases:

- EMBL:   AE006470
- RefSeq:   NP_663134.1
- ProteinModelPortal:   Q8KAA5
- SMR:   Q8KAA5
- GeneID:   1007298
- GenomeReviews:   AE006470_GR
- KEGG:   cte:CT2261
- NMPDR:   fig|194439.1.peg.2228
- TIGR:   CT2261
- HOGENOM:   HBG584843
- OMA:   RLGPTPI
- ProtClustDB:   PRK00015
- BioCyc:   CTEP194439:CT_2261-MONOMER
- BRENDA:   3.1.26.4
- GO:   GO:0005737
- HAMAP:   MF_00052_B
- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337
- PANTHER:   PTHR10954

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 22583; Mature: 22583

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSTDFEYPLWESLSQVCGIDEAGRGPLAGPVVAAAVVFPRHFRPTGIFAKLDDSKKLTA
CCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCHHHHH
ELRDELALAIRESAESWALGVVDAETIDRINILQATMLAMNLAVESLGSTPEFLLVDGNR
HHHHHHHHHHHHCCCHHEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCC
FRPVLPIPYQTIVKGDSKVFSIAAASVLAKTHRDELMTTSAAEYPEYGFEVHFGYPTARH
CCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCHHH
VEAIARHGRCAIHRQSFKLRKLGEK
HHHHHHCCCEEEEHHHHHHHHCCCC
>Mature Secondary Structure
MLSTDFEYPLWESLSQVCGIDEAGRGPLAGPVVAAAVVFPRHFRPTGIFAKLDDSKKLTA
CCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCHHHHH
ELRDELALAIRESAESWALGVVDAETIDRINILQATMLAMNLAVESLGSTPEFLLVDGNR
HHHHHHHHHHHHCCCHHEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCC
FRPVLPIPYQTIVKGDSKVFSIAAASVLAKTHRDELMTTSAAEYPEYGFEVHFGYPTARH
CCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCHHH
VEAIARHGRCAIHRQSFKLRKLGEK
HHHHHHCCCEEEEHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12093901