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The map label for this gene is glyS

Identifier: 21675063

GI number: 21675063

Start: 2117985

End: 2119397

Strand: Direct

Name: glyS

Synonym: CT2255

Alternate gene names: 21675063

Gene position: 2117985-2119397 (Clockwise)

Preceding gene: 21675062

Following gene: 21675064

Centisome position: 98.28

GC content: 59.31

Gene sequence:

>1413_bases
ATGAACAAGCTGGTTTCGCTGGCCAAGCGGCGGGGCTTTATTTTTCCGTCGTCGGAGATTTATGGCGGCCTGTCGTCGTG
CTTCGATTACGGCCCGCTCGGCAGCGAAATGAAGAAGAATATTAAGGATTTGTGGTGGAACGCTATGACGCGCCGCCATC
AGAACATCGTGGGGATCGATGCGTCGATCATGATGAATCCGACCGTGTGGGAGGCTTCGGGCCATGTGGCAAGCTTCAAC
GATCCGATGATCGACGACAAAACCACCAAGCGCCGCTACCGCGCCGACCACCTGATCGAAAACCATATCGAGAAGCTCCA
TCGCGACGGCAAGGAGGCAGAGGCCGCCGCCATCAAGGTTGCCTATGAGGCCGCCGGGGGCACCGAAGATCCGAACCGCA
CATTGTATAATATTATTATTGAGGCGGGTATCAAGGCTCCCGACACCGGTTCGGCGGACTGGACGGAGGTGCGTCAATTC
AACCTGATGTTCCAGTGCAACATGGGCGCGGTGGCCGATTCAGCGGGCGTGGTCTATCTGCGGCCCGAAACCGCGCAGGG
CATCTTTGTAAACTTCCACAACGTCCGTGAAGCATCGCGCATGAAGGTGCCGTTCGGCATCGCGCAGATCGGCAAGGCGT
TCCGTAACGAGATCGTCAAGGGCAACTTCATCTTCCGCATGGTGGAGTTCGAGCAGATGGAGATGCAGTATTTCGTCAAG
CCCGGCACGCAGCTCGAAGCGTTCGAGGCGTGGCGCGAGGAGCGCTTCCGCTGGTACTCCGAAACGCTCGGCATGTCGAA
GGAGAAACTGCACTGGTACAAGCACGACAAGCTGGCCCACTACGCCGACCTGGCCTACGACATCAAGTTCGAGTTCCCGT
TCGGCATTGAGGAGATCGAAGGCATCCACTCGCGCACCGATTTCGACCTGAGCCAGCATCAGAAGTACTCCGGCAAGAGC
ATGGAGTACATCGACCAGACCACCAACGAGCGCTACATTCCCTACGTCGTCGAGACCTCGTCGGGCTGCGACCGCACCTT
CCTGGCGCTGCTGTCGGACGCCTATCAGGAGGATGTGGTGGACGGCGAGCCGCGCGTGATGCTGAAGCTCGCGCCGAAGG
TTGCGCCGGTGAAGGCCGCCGTGCTGCCGCTGATGAAGAAGGGCGAAATGGGCGAAAAGGCCGCGCAGCTTTGCCGAGAC
CTGTCGGAGAGCTTCATGGTGCAGTACGACGACGCAGCCTCGATCGGCAAGCGCTACCGCCGTCAGGACGAGATCGGCAC
GCCGTTCTGCTTCACGGTCGATCACGACACACTGGAGAACGGCACCATTACCGTGCGCTACCGCGACACGGCGGCGCAGG
AGCGGATCAATATGTCGAAAGCCGCTGAGTTCCTTGCGACCAAACTAATGTAA

Upstream 100 bases:

>100_bases
ACGGGTGGGAAACGCTGAATTTTTCAACTTCTCAGATCAAACGATGAGCAATTCCGATCAGTCGCGGGTGCAGACGCAGA
ATCTGTCGCCCGATAAAGTG

Downstream 100 bases:

>100_bases
CGCGGCATGAAGAGATTGGGCTAACACCCATAAATAAATTGCGTTATCCATGAACCCCGGACTAAAGTCCAGGGCAATTG
TTAAAAATTTTTTACTGGAT

Product: glycyl-tRNA synthetase

Products: NA

Alternate protein names: Glycine--tRNA ligase; GlyRS

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MNKLVSLAKRRGFIFPSSEIYGGLSSCFDYGPLGSEMKKNIKDLWWNAMTRRHQNIVGIDASIMMNPTVWEASGHVASFN
DPMIDDKTTKRRYRADHLIENHIEKLHRDGKEAEAAAIKVAYEAAGGTEDPNRTLYNIIIEAGIKAPDTGSADWTEVRQF
NLMFQCNMGAVADSAGVVYLRPETAQGIFVNFHNVREASRMKVPFGIAQIGKAFRNEIVKGNFIFRMVEFEQMEMQYFVK
PGTQLEAFEAWREERFRWYSETLGMSKEKLHWYKHDKLAHYADLAYDIKFEFPFGIEEIEGIHSRTDFDLSQHQKYSGKS
MEYIDQTTNERYIPYVVETSSGCDRTFLALLSDAYQEDVVDGEPRVMLKLAPKVAPVKAAVLPLMKKGEMGEKAAQLCRD
LSESFMVQYDDAASIGKRYRRQDEIGTPFCFTVDHDTLENGTITVRYRDTAAQERINMSKAAEFLATKLM

Sequences:

>Translated_470_residues
MNKLVSLAKRRGFIFPSSEIYGGLSSCFDYGPLGSEMKKNIKDLWWNAMTRRHQNIVGIDASIMMNPTVWEASGHVASFN
DPMIDDKTTKRRYRADHLIENHIEKLHRDGKEAEAAAIKVAYEAAGGTEDPNRTLYNIIIEAGIKAPDTGSADWTEVRQF
NLMFQCNMGAVADSAGVVYLRPETAQGIFVNFHNVREASRMKVPFGIAQIGKAFRNEIVKGNFIFRMVEFEQMEMQYFVK
PGTQLEAFEAWREERFRWYSETLGMSKEKLHWYKHDKLAHYADLAYDIKFEFPFGIEEIEGIHSRTDFDLSQHQKYSGKS
MEYIDQTTNERYIPYVVETSSGCDRTFLALLSDAYQEDVVDGEPRVMLKLAPKVAPVKAAVLPLMKKGEMGEKAAQLCRD
LSESFMVQYDDAASIGKRYRRQDEIGTPFCFTVDHDTLENGTITVRYRDTAAQERINMSKAAEFLATKLM
>Mature_470_residues
MNKLVSLAKRRGFIFPSSEIYGGLSSCFDYGPLGSEMKKNIKDLWWNAMTRRHQNIVGIDASIMMNPTVWEASGHVASFN
DPMIDDKTTKRRYRADHLIENHIEKLHRDGKEAEAAAIKVAYEAAGGTEDPNRTLYNIIIEAGIKAPDTGSADWTEVRQF
NLMFQCNMGAVADSAGVVYLRPETAQGIFVNFHNVREASRMKVPFGIAQIGKAFRNEIVKGNFIFRMVEFEQMEMQYFVK
PGTQLEAFEAWREERFRWYSETLGMSKEKLHWYKHDKLAHYADLAYDIKFEFPFGIEEIEGIHSRTDFDLSQHQKYSGKS
MEYIDQTTNERYIPYVVETSSGCDRTFLALLSDAYQEDVVDGEPRVMLKLAPKVAPVKAAVLPLMKKGEMGEKAAQLCRD
LSESFMVQYDDAASIGKRYRRQDEIGTPFCFTVDHDTLENGTITVRYRDTAAQERINMSKAAEFLATKLM

Specific function: Catalyzes the attachment of glycine to tRNA(Gly)

COG id: COG0423

COG function: function code J; Glycyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI116805340, Length=361, Percent_Identity=31.3019390581717, Blast_Score=166, Evalue=4e-41,
Organism=Homo sapiens, GI70887790, Length=305, Percent_Identity=25.5737704918033, Blast_Score=86, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI32564541, Length=404, Percent_Identity=31.4356435643564, Blast_Score=171, Evalue=6e-43,
Organism=Caenorhabditis elegans, GI17554996, Length=404, Percent_Identity=31.4356435643564, Blast_Score=171, Evalue=7e-43,
Organism=Saccharomyces cerevisiae, GI6325338, Length=371, Percent_Identity=31.266846361186, Blast_Score=161, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6319597, Length=400, Percent_Identity=27.25, Blast_Score=131, Evalue=3e-31,
Organism=Drosophila melanogaster, GI21357965, Length=364, Percent_Identity=32.967032967033, Blast_Score=172, Evalue=3e-43,
Organism=Drosophila melanogaster, GI24664462, Length=364, Percent_Identity=32.967032967033, Blast_Score=172, Evalue=5e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYG_CHLTE (Q8KAB1)

Other databases:

- EMBL:   AE006470
- RefSeq:   NP_663128.1
- ProteinModelPortal:   Q8KAB1
- SMR:   Q8KAB1
- GeneID:   1007304
- GenomeReviews:   AE006470_GR
- KEGG:   cte:CT2255
- NMPDR:   fig|194439.1.peg.2222
- TIGR:   CT2255
- HOGENOM:   HBG319978
- OMA:   GSEWGEL
- ProtClustDB:   PRK04173
- BioCyc:   CTEP194439:CT_2255-MONOMER
- BRENDA:   6.1.1.14
- GO:   GO:0005737
- HAMAP:   MF_00253_B
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR022961
- InterPro:   IPR002315
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR10745
- PRINTS:   PR01043
- TIGRFAMs:   TIGR00389

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.14

Molecular weight: Translated: 53719; Mature: 53719

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: BINDING 94-94 BINDING 183-183

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKLVSLAKRRGFIFPSSEIYGGLSSCFDYGPLGSEMKKNIKDLWWNAMTRRHQNIVGID
CCHHHHHHHHCCCCCCCHHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCEEECC
ASIMMNPTVWEASGHVASFNDPMIDDKTTKRRYRADHLIENHIEKLHRDGKEAEAAAIKV
CEEEECCEEEECCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHEEEE
AYEAAGGTEDPNRTLYNIIIEAGIKAPDTGSADWTEVRQFNLMFQCNMGAVADSAGVVYL
EEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHEEEEEEEECCCCEECCCCEEEE
RPETAQGIFVNFHNVREASRMKVPFGIAQIGKAFRNEIVKGNFIFRMVEFEQMEMQYFVK
CCCCCCEEEEEEHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHEEC
PGTQLEAFEAWREERFRWYSETLGMSKEKLHWYKHDKLAHYADLAYDIKFEFPFGIEEIE
CCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCEEEEEEEECCCCHHHHH
GIHSRTDFDLSQHQKYSGKSMEYIDQTTNERYIPYVVETSSGCDRTFLALLSDAYQEDVV
HHHCCCCCCHHHHHCCCCCCHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCC
DGEPRVMLKLAPKVAPVKAAVLPLMKKGEMGEKAAQLCRDLSESFMVQYDDAASIGKRYR
CCCCEEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEEEECCHHHHHHHHH
RQDEIGTPFCFTVDHDTLENGTITVRYRDTAAQERINMSKAAEFLATKLM
HHHCCCCCEEEEECCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNKLVSLAKRRGFIFPSSEIYGGLSSCFDYGPLGSEMKKNIKDLWWNAMTRRHQNIVGID
CCHHHHHHHHCCCCCCCHHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCEEECC
ASIMMNPTVWEASGHVASFNDPMIDDKTTKRRYRADHLIENHIEKLHRDGKEAEAAAIKV
CEEEECCEEEECCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHEEEE
AYEAAGGTEDPNRTLYNIIIEAGIKAPDTGSADWTEVRQFNLMFQCNMGAVADSAGVVYL
EEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHEEEEEEEECCCCEECCCCEEEE
RPETAQGIFVNFHNVREASRMKVPFGIAQIGKAFRNEIVKGNFIFRMVEFEQMEMQYFVK
CCCCCCEEEEEEHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHEEC
PGTQLEAFEAWREERFRWYSETLGMSKEKLHWYKHDKLAHYADLAYDIKFEFPFGIEEIE
CCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCEEEEEEEECCCCHHHHH
GIHSRTDFDLSQHQKYSGKSMEYIDQTTNERYIPYVVETSSGCDRTFLALLSDAYQEDVV
HHHCCCCCCHHHHHCCCCCCHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCC
DGEPRVMLKLAPKVAPVKAAVLPLMKKGEMGEKAAQLCRDLSESFMVQYDDAASIGKRYR
CCCCEEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEEEECCHHHHHHHHH
RQDEIGTPFCFTVDHDTLENGTITVRYRDTAAQERINMSKAAEFLATKLM
HHHCCCCCEEEEECCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12093901