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The map label for this gene is yeiT [H]

Identifier: 21675056

GI number: 21675056

Start: 2109133

End: 2110866

Strand: Reverse

Name: yeiT [H]

Synonym: CT2247

Alternate gene names: 21675056

Gene position: 2110866-2109133 (Counterclockwise)

Preceding gene: 21675057

Following gene: 21675055

Centisome position: 97.95

GC content: 62.75

Gene sequence:

>1734_bases
ATGAATGCAGAATCAAACCCGATTCTCGATTTTGCGACAGAGTACGTCTACCCTGCCTTCAGCGAACTGACAGGGACCGA
CAAGATCGTCGCCTTTGGGGATCACAGCCACAAATGCCCGATCTACGTACCGCAGACCCCGCCCTGCACCGCCGAGTGCC
CGGCAGGCGAAGACATCCGGGCCATCAACCGTTTTCTGAACGGCACCGACCCGTCGGACGACCCGCTGAAATCGGCCTGG
GAAACGGCCACCGACACCAACCCCTTCCCGGCGGTAATGGGGCGCATCTGCCCGCACCCGTGCCAGAGCAAATGCAACCG
CGGCGTGCACGACGAAAGCGTGGCCATCAACGCCGTCGAGCAGGTGCTCGGCAACTACGGCATCGAGCACAACCTCAAAC
TCAAAGGCCCCGGTGCCGACACCGGCAAGCGGGTCGCCATCATCGGCGGCGGCCCGGCGGGACTCTCCGCCGCCTACCAG
CTGCGCCGCAAAGGCCACGCCGTCACCATCTACGACGCCAACGAAAAGCTCGGCGGCATGGTGCTCTATGGCATCATGGG
CTACCGCGTAGATCGCAAAGTGCTCGAAGCCGAAATCGGGCGCATCATCGAACTCGGCGTCGAGACGAAGATGGGCGTCA
CCATCGGCAAGGACATCACCCTCGAACAGCTCGAAGCGGAGTACGACGCGGTCTTCATCGCCGTCGGCGCACAGAAGGGA
AGAGCTCTTCCCGTGCCCGGCTTTGAAGGCACGCCCGGCGCCACCAACGCCATCGACTTCCTGAAAAGCTACGAAGTGCT
GGGCGATGACATTCCGGTCGGCAAGCACGTGGTGGTGATCGGCGACGGTAACGTAGCGATGGACGTCGCGCGTCTGGCGC
TGCGTCTCGGCTCGCAAGCCACCATCATCTCCGGCGTGCCGCGCGAAGAGATGGCCTGCTTCGAGAACGAATTCGACGAC
GCCAAAAACGAAGGCACAACGATGCACTTTCTCACCGGCACGGTAGAAGTGCTCGGCGGAGCGAGTGGCGTCACCGGCCT
GCGTTGCACAAAGATGGTCAAAAAGGAGAAAGGCGAGGAAGGCTGGAACTCCCCGATTCCGTTCCTGCGCTACAAAAGCA
ATGGCGAAAGCTTCGAGATCGAAGCGGACATGGTGGTGGCGGCCATCGGCCAGGCGACCGACCTGTCGGGCCTCGGCAGT
GCGGCCAGCGGCCCGTGGCTCAAGGTTGACCGCAACTTCCGGATTCCGGGCCGCGAAAAGCTCTTCGGCGGCGGCGACGC
CCTGAAGGTCGATCTCATCACCACGGCGGTGGGTCACGGCCGCAAAGCCGCCTACGCCATCGACGCCTTCCTGAAAGGCG
AACCGATGCCGGAGGAGCCGTATCGCGAGATCACCAAGCCGCACAAGCAGGACCTGCTCTACTTCCTGCACACCCCGCAG
GCAAAACGCACCAGCATCAAGCCGGAGGTGGTGGTGGGCAACCACGACGAACTGCTCGAAGCGCTGACGCCCGAACAGGC
CATCACCGAATCGAAGCGGTGTATGAGCTGCGGGTTCTGCTTCGACTGCAAGCAGTGCGTCTCGTTCTGCCCGCAGGAGG
CGATCACCCGCTTCCGCGACAACCCGGCAGGCGAAAAAGTCTATACCGACTACACAAAATGCGTCGGCTGCCACCTCTGC
TCCCTGGTCTGCCCCTGCGGCTACATCCAGATGGGCATGGGCGACGGACTGTAA

Upstream 100 bases:

>100_bases
GTTCGACGACAACATGATCGACAGCTACCGTCACGCCCGAACCACCTTCAACCAGTCGGCGCACATCCGTTTTTAAGCTG
AAAAGCAAAGGAGCAACCTC

Downstream 100 bases:

>100_bases
ATTGGGCGGCATCGTAACCTGAAAGACCGAAACACTATGATGGACGATATGATCCAGATGGTCGAGAAGGCCATCGAAAC
ATCCTTGCATTGGCAGGAGA

Product: putative glutamate synthase (NADPH) small subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 577; Mature: 577

Protein sequence:

>577_residues
MNAESNPILDFATEYVYPAFSELTGTDKIVAFGDHSHKCPIYVPQTPPCTAECPAGEDIRAINRFLNGTDPSDDPLKSAW
ETATDTNPFPAVMGRICPHPCQSKCNRGVHDESVAINAVEQVLGNYGIEHNLKLKGPGADTGKRVAIIGGGPAGLSAAYQ
LRRKGHAVTIYDANEKLGGMVLYGIMGYRVDRKVLEAEIGRIIELGVETKMGVTIGKDITLEQLEAEYDAVFIAVGAQKG
RALPVPGFEGTPGATNAIDFLKSYEVLGDDIPVGKHVVVIGDGNVAMDVARLALRLGSQATIISGVPREEMACFENEFDD
AKNEGTTMHFLTGTVEVLGGASGVTGLRCTKMVKKEKGEEGWNSPIPFLRYKSNGESFEIEADMVVAAIGQATDLSGLGS
AASGPWLKVDRNFRIPGREKLFGGGDALKVDLITTAVGHGRKAAYAIDAFLKGEPMPEEPYREITKPHKQDLLYFLHTPQ
AKRTSIKPEVVVGNHDELLEALTPEQAITESKRCMSCGFCFDCKQCVSFCPQEAITRFRDNPAGEKVYTDYTKCVGCHLC
SLVCPCGYIQMGMGDGL

Sequences:

>Translated_577_residues
MNAESNPILDFATEYVYPAFSELTGTDKIVAFGDHSHKCPIYVPQTPPCTAECPAGEDIRAINRFLNGTDPSDDPLKSAW
ETATDTNPFPAVMGRICPHPCQSKCNRGVHDESVAINAVEQVLGNYGIEHNLKLKGPGADTGKRVAIIGGGPAGLSAAYQ
LRRKGHAVTIYDANEKLGGMVLYGIMGYRVDRKVLEAEIGRIIELGVETKMGVTIGKDITLEQLEAEYDAVFIAVGAQKG
RALPVPGFEGTPGATNAIDFLKSYEVLGDDIPVGKHVVVIGDGNVAMDVARLALRLGSQATIISGVPREEMACFENEFDD
AKNEGTTMHFLTGTVEVLGGASGVTGLRCTKMVKKEKGEEGWNSPIPFLRYKSNGESFEIEADMVVAAIGQATDLSGLGS
AASGPWLKVDRNFRIPGREKLFGGGDALKVDLITTAVGHGRKAAYAIDAFLKGEPMPEEPYREITKPHKQDLLYFLHTPQ
AKRTSIKPEVVVGNHDELLEALTPEQAITESKRCMSCGFCFDCKQCVSFCPQEAITRFRDNPAGEKVYTDYTKCVGCHLC
SLVCPCGYIQMGMGDGL
>Mature_577_residues
MNAESNPILDFATEYVYPAFSELTGTDKIVAFGDHSHKCPIYVPQTPPCTAECPAGEDIRAINRFLNGTDPSDDPLKSAW
ETATDTNPFPAVMGRICPHPCQSKCNRGVHDESVAINAVEQVLGNYGIEHNLKLKGPGADTGKRVAIIGGGPAGLSAAYQ
LRRKGHAVTIYDANEKLGGMVLYGIMGYRVDRKVLEAEIGRIIELGVETKMGVTIGKDITLEQLEAEYDAVFIAVGAQKG
RALPVPGFEGTPGATNAIDFLKSYEVLGDDIPVGKHVVVIGDGNVAMDVARLALRLGSQATIISGVPREEMACFENEFDD
AKNEGTTMHFLTGTVEVLGGASGVTGLRCTKMVKKEKGEEGWNSPIPFLRYKSNGESFEIEADMVVAAIGQATDLSGLGS
AASGPWLKVDRNFRIPGREKLFGGGDALKVDLITTAVGHGRKAAYAIDAFLKGEPMPEEPYREITKPHKQDLLYFLHTPQ
AKRTSIKPEVVVGNHDELLEALTPEQAITESKRCMSCGFCFDCKQCVSFCPQEAITRFRDNPAGEKVYTDYTKCVGCHLC
SLVCPCGYIQMGMGDGL

Specific function: Unknown

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To E.coli glutamate synthase [NADPH] small chain (GltD) and to E.coli AegA [H]

Homologues:

Organism=Homo sapiens, GI111118983, Length=158, Percent_Identity=31.6455696202532, Blast_Score=69, Evalue=2e-11,
Organism=Homo sapiens, GI111118981, Length=158, Percent_Identity=31.6455696202532, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1788468, Length=417, Percent_Identity=33.3333333333333, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI1788811, Length=411, Percent_Identity=30.9002433090024, Blast_Score=162, Evalue=6e-41,
Organism=Escherichia coli, GI1789243, Length=535, Percent_Identity=29.1588785046729, Blast_Score=155, Evalue=7e-39,
Organism=Escherichia coli, GI87082180, Length=438, Percent_Identity=28.9954337899543, Blast_Score=152, Evalue=6e-38,
Organism=Escherichia coli, GI1789606, Length=441, Percent_Identity=29.4784580498866, Blast_Score=145, Evalue=8e-36,
Organism=Caenorhabditis elegans, GI17570289, Length=448, Percent_Identity=28.3482142857143, Blast_Score=139, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI71984108, Length=462, Percent_Identity=25.1082251082251, Blast_Score=82, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17543860, Length=195, Percent_Identity=32.8205128205128, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6320030, Length=272, Percent_Identity=31.6176470588235, Blast_Score=137, Evalue=6e-33,
Organism=Saccharomyces cerevisiae, GI6320584, Length=174, Percent_Identity=30.4597701149425, Blast_Score=64, Evalue=7e-11,
Organism=Drosophila melanogaster, GI28574881, Length=456, Percent_Identity=28.0701754385965, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24665539, Length=456, Percent_Identity=28.0701754385965, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24665547, Length=456, Percent_Identity=28.2894736842105, Blast_Score=156, Evalue=4e-38,
Organism=Drosophila melanogaster, GI24665543, Length=456, Percent_Identity=28.2894736842105, Blast_Score=156, Evalue=4e-38,
Organism=Drosophila melanogaster, GI24640763, Length=446, Percent_Identity=24.6636771300448, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI18858217, Length=446, Percent_Identity=24.6636771300448, Blast_Score=96, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: NA

Molecular weight: Translated: 62208; Mature: 62208

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAESNPILDFATEYVYPAFSELTGTDKIVAFGDHSHKCPIYVPQTPPCTAECPAGEDIR
CCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEECCCCCCCCCCCCCCCHHH
AINRFLNGTDPSDDPLKSAWETATDTNPFPAVMGRICPHPCQSKCNRGVHDESVAINAVE
HHHHHHCCCCCCCCHHHHHHHCCCCCCCHHHHHHHCCCCHHHHHHCCCCCCCHHHHHHHH
QVLGNYGIEHNLKLKGPGADTGKRVAIIGGGPAGLSAAYQLRRKGHAVTIYDANEKLGGM
HHHHCCCCCCEEEEECCCCCCCCEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCCCCE
VLYGIMGYRVDRKVLEAEIGRIIELGVETKMGVTIGKDITLEQLEAEYDAVFIAVGAQKG
EEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHCCCCEEEEEEECCCC
RALPVPGFEGTPGATNAIDFLKSYEVLGDDIPVGKHVVVIGDGNVAMDVARLALRLGSQA
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCE
TIISGVPREEMACFENEFDDAKNEGTTMHFLTGTVEVLGGASGVTGLRCTKMVKKEKGEE
EEECCCCHHHHHHHHHHHCCCCCCCCEEEEEEEEHHHHCCCCCCCHHHHHHHHHHHCCCC
GWNSPIPFLRYKSNGESFEIEADMVVAAIGQATDLSGLGSAASGPWLKVDRNFRIPGREK
CCCCCCCEEEECCCCCEEEEECCEEEEECCCCCCCCCCCCCCCCCEEEECCCCCCCCHHH
LFGGGDALKVDLITTAVGHGRKAAYAIDAFLKGEPMPEEPYREITKPHKQDLLYFLHTPQ
CCCCCCEEEEEEEEHHHCCCCCHHHEEHHHHCCCCCCCCHHHHHCCCCHHCEEEEEECCC
AKRTSIKPEVVVGNHDELLEALTPEQAITESKRCMSCGFCFDCKQCVSFCPQEAITRFRD
CHHCCCCCEEEECCHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHCC
NPAGEKVYTDYTKCVGCHLCSLVCPCGYIQMGMGDGL
CCCCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCCCC
>Mature Secondary Structure
MNAESNPILDFATEYVYPAFSELTGTDKIVAFGDHSHKCPIYVPQTPPCTAECPAGEDIR
CCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEECCCCCCCCCCCCCCCHHH
AINRFLNGTDPSDDPLKSAWETATDTNPFPAVMGRICPHPCQSKCNRGVHDESVAINAVE
HHHHHHCCCCCCCCHHHHHHHCCCCCCCHHHHHHHCCCCHHHHHHCCCCCCCHHHHHHHH
QVLGNYGIEHNLKLKGPGADTGKRVAIIGGGPAGLSAAYQLRRKGHAVTIYDANEKLGGM
HHHHCCCCCCEEEEECCCCCCCCEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCCCCE
VLYGIMGYRVDRKVLEAEIGRIIELGVETKMGVTIGKDITLEQLEAEYDAVFIAVGAQKG
EEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHCCCCEEEEEEECCCC
RALPVPGFEGTPGATNAIDFLKSYEVLGDDIPVGKHVVVIGDGNVAMDVARLALRLGSQA
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCE
TIISGVPREEMACFENEFDDAKNEGTTMHFLTGTVEVLGGASGVTGLRCTKMVKKEKGEE
EEECCCCHHHHHHHHHHHCCCCCCCCEEEEEEEEHHHHCCCCCCCHHHHHHHHHHHCCCC
GWNSPIPFLRYKSNGESFEIEADMVVAAIGQATDLSGLGSAASGPWLKVDRNFRIPGREK
CCCCCCCEEEECCCCCEEEEECCEEEEECCCCCCCCCCCCCCCCCEEEECCCCCCCCHHH
LFGGGDALKVDLITTAVGHGRKAAYAIDAFLKGEPMPEEPYREITKPHKQDLLYFLHTPQ
CCCCCCEEEEEEEEHHHCCCCCHHHEEHHHHCCCCCCCCHHHHHCCCCHHCEEEEEECCC
AKRTSIKPEVVVGNHDELLEALTPEQAITESKRCMSCGFCFDCKQCVSFCPQEAITRFRD
CHHCCCCCEEEECCHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHCC
NPAGEKVYTDYTKCVGCHLCSLVCPCGYIQMGMGDGL
CCCCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]