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The map label for this gene is hmeA [H]

Identifier: 21675050

GI number: 21675050

Start: 2104360

End: 2105136

Strand: Reverse

Name: hmeA [H]

Synonym: CT2241

Alternate gene names: 21675050

Gene position: 2105136-2104360 (Counterclockwise)

Preceding gene: 21675051

Following gene: 21675049

Centisome position: 97.69

GC content: 60.36

Gene sequence:

>777_bases
ATGAGTCAAAACCGTAGAGAATTTCTGAAAAAGGCCGGTCTGGGCGCTCTCGCCGGCCTGGGTGCCGCCGCGGGTCTGTT
CCCGGCGTTCGCGCTTGAGAATACCGATATGTCGAGCTTTACCACCAAGCCGACGCCGGGCAAGGTGCGCTGGGGAATGC
TGGTTGATACCCGCAAGTGCCTCGGCGACTGCAAGGAGTGCATCGTCCGTTGCCACCACGATCACAACGTGCCGGACTTC
GGCCATACGAAGAACGAGGTCAAATGGATATGGAAGAGCGGTTATGAGAATGCGTTTCCGACCGCCAGCACGCAGTTCCA
GAATCCCGAAGTGCTGGAACGCCAGGTGCTGACGCTCTGCAACCACTGCACCGAGCCGCCCTGCACCAAAGCCTGCCCGA
CCGAGGCGACCTTTAAGCGCTGGGACGGCATCGTCTCCATCGACTACCATCGCTGCATTGGCTGCCGCTTCTGCATGGCC
GCCTGCCCGTACGGTTCGCGCAGCTTCAACTGGCTCGATCCGCGTCCGCACATCAAGGAGCTGAGCAACACCTACCCGAC
ACGGATGCGCGGCGTGGTCGAGAAGTGCAACTTCTGCTCGGAGCGGCTCGTCAAGGGCGAGCTTCCGGCCTGCGTCGTCA
GCTGTGCGGAGAATGCGCTTACCTTCGGCGACCTGAACGATCCAAATTCGGAGATCCGCAAGATGCTCGCCACGAACGAA
ACCATGCAGCGCAAGCCGGAACTCGGCACGCTGCCATCAGTCTTTTACATCATCTAA

Upstream 100 bases:

>100_bases
GCCACGGCTCGAATCCAATGTTCTGTTTTATGTGTCACGAGTATGCCAATGTCAAGCCGACATGCTGGAACTGTCATCTG
TCGCCGATGGAGGTGTCGCA

Downstream 100 bases:

>100_bases
CCATAAGCCATGATCGAGAAAGCGCTTAAAGGAGGCCGCGGCTACTGGACATGGGTAGCCTTCCTGCTCGTTGTCATCGG
GCTTGGAGTCTCGGCGTACG

Product: polysulfide reductase, subunit B, putative

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: Hme subunit A [H]

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MSQNRREFLKKAGLGALAGLGAAAGLFPAFALENTDMSSFTTKPTPGKVRWGMLVDTRKCLGDCKECIVRCHHDHNVPDF
GHTKNEVKWIWKSGYENAFPTASTQFQNPEVLERQVLTLCNHCTEPPCTKACPTEATFKRWDGIVSIDYHRCIGCRFCMA
ACPYGSRSFNWLDPRPHIKELSNTYPTRMRGVVEKCNFCSERLVKGELPACVVSCAENALTFGDLNDPNSEIRKMLATNE
TMQRKPELGTLPSVFYII

Sequences:

>Translated_258_residues
MSQNRREFLKKAGLGALAGLGAAAGLFPAFALENTDMSSFTTKPTPGKVRWGMLVDTRKCLGDCKECIVRCHHDHNVPDF
GHTKNEVKWIWKSGYENAFPTASTQFQNPEVLERQVLTLCNHCTEPPCTKACPTEATFKRWDGIVSIDYHRCIGCRFCMA
ACPYGSRSFNWLDPRPHIKELSNTYPTRMRGVVEKCNFCSERLVKGELPACVVSCAENALTFGDLNDPNSEIRKMLATNE
TMQRKPELGTLPSVFYII
>Mature_257_residues
SQNRREFLKKAGLGALAGLGAAAGLFPAFALENTDMSSFTTKPTPGKVRWGMLVDTRKCLGDCKECIVRCHHDHNVPDFG
HTKNEVKWIWKSGYENAFPTASTQFQNPEVLERQVLTLCNHCTEPPCTKACPTEATFKRWDGIVSIDYHRCIGCRFCMAA
CPYGSRSFNWLDPRPHIKELSNTYPTRMRGVVEKCNFCSERLVKGELPACVVSCAENALTFGDLNDPNSEIRKMLATNET
MQRKPELGTLPSVFYII

Specific function: Has menaquinol-oxidizing activity. HmeA, hmeB and hmeE subunits may together catalyze electron transfer from menaquinol to cytochrome c [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Extracellular side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Escherichia coli, GI2367345, Length=259, Percent_Identity=33.5907335907336, Blast_Score=124, Evalue=8e-30,
Organism=Escherichia coli, GI1787122, Length=186, Percent_Identity=32.7956989247312, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787872, Length=190, Percent_Identity=32.6315789473684, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1789370, Length=253, Percent_Identity=30.8300395256917, Blast_Score=98, Evalue=4e-22,
Organism=Escherichia coli, GI226510944, Length=257, Percent_Identity=31.5175097276265, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI1790326, Length=198, Percent_Identity=31.8181818181818, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1787749, Length=208, Percent_Identity=30.2884615384615, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787478, Length=132, Percent_Identity=32.5757575757576, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI1787740, Length=122, Percent_Identity=34.4262295081967, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788811, Length=170, Percent_Identity=24.7058823529412, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1789067, Length=178, Percent_Identity=28.6516853932584, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 28964; Mature: 28832

Theoretical pI: Translated: 8.13; Mature: 8.13

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

6.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
8.9 %Cys+Met (Translated Protein)
6.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
8.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQNRREFLKKAGLGALAGLGAAAGLFPAFALENTDMSSFTTKPTPGKVRWGMLVDTRKC
CCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECHHHHHHHH
LGDCKECIVRCHHDHNVPDFGHTKNEVKWIWKSGYENAFPTASTQFQNPEVLERQVLTLC
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHH
NHCTEPPCTKACPTEATFKRWDGIVSIDYHRCIGCRFCMAACPYGSRSFNWLDPRPHIKE
HHCCCCCCCCCCCCHHHHHHHCCCEEECHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHH
LSNTYPTRMRGVVEKCNFCSERLVKGELPACVVSCAENALTFGDLNDPNSEIRKMLATNE
HHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCEECCCCCCCHHHHHHHHHHCH
TMQRKPELGTLPSVFYII
HHHHCCCCCCCCCHHHCC
>Mature Secondary Structure 
SQNRREFLKKAGLGALAGLGAAAGLFPAFALENTDMSSFTTKPTPGKVRWGMLVDTRKC
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECHHHHHHHH
LGDCKECIVRCHHDHNVPDFGHTKNEVKWIWKSGYENAFPTASTQFQNPEVLERQVLTLC
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHH
NHCTEPPCTKACPTEATFKRWDGIVSIDYHRCIGCRFCMAACPYGSRSFNWLDPRPHIKE
HHCCCCCCCCCCCCHHHHHHHCCCEEECHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHH
LSNTYPTRMRGVVEKCNFCSERLVKGELPACVVSCAENALTFGDLNDPNSEIRKMLATNE
HHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCEECCCCCCCHHHHHHHHHHCH
TMQRKPELGTLPSVFYII
HHHHCCCCCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9389475; 11952791 [H]