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The map label for this gene is ykfA [H]

Identifier: 21675045

GI number: 21675045

Start: 2099664

End: 2100611

Strand: Direct

Name: ykfA [H]

Synonym: CT2236

Alternate gene names: 21675045

Gene position: 2099664-2100611 (Clockwise)

Preceding gene: 21675044

Following gene: 21675046

Centisome position: 97.43

GC content: 59.49

Gene sequence:

>948_bases
ATGAACATTCTGATCCCCAAAGCGCTCCGCAGGGGAGAGGTCATCGGCCTGATCTCGCCATCTTCGACCTGCGCCGAGCC
GGAAAAAATCGAACGGGCCGTCACCTACCTTGAGCGCTGTGGCTACAAGGTCAAGACCTCGCTGTACCTGAACCGCTCAG
AGCACGACCCCGCGCATACCGACCGGTACAAGCTTCACGATCTTCACCAGATGTTCGCAGATCGTGAAGTACGGGCTATT
TTCTGCCTCCGGGGCGGTGCCGGAGCGACACGTCTGCTTGACCGTATTGACTACGGACTGATTGCGGCCAATCCGAAAAT
CCTTGTCGGCTATTCCGATATCACGGCACTTTCTCTGGCCGTGTTCAGGAAAACCGGCCTCGTCAACTTTTCCGGCCCCA
TGGCCGCCACGGAGCTTTTGGCGCCCAGCAGCTACACCGAAGAACATTTCTGGGGAATGCTGACCGATCCGGGTTATTCG
AAGCACCTCACCAATTTCAGCGAACACCCGATCAGTTGCATCAGACCAGGAGCCGTCACCGGCAGGCTTGTTGGAGGCAA
TCTCTCGGTGCTCTCCTCGCTGGTCGGCACGCCGTACCTGCCCTCGTTCAGCGGCGCGCTGCTCTTCACCGAAGATGTCA
ACGAACCGGCCTACCGCATCGACCGGATGCTCTCGCACCTCTTCAACGCCGGTCTGGCGCAGAAGTGCCGGGGCCTGATG
TTCGGCCAGTTCAGCAAAAATCCCGCCGACGAAAACCGCGATTACCGCTTCGACAAGATGTTTACCTATTACGCAAACCG
GATGCACGACGGCGTGCCGGTGATGACCGGCCTCTCCTATGGCCACATCCGGGAGCTGATGACCCTGCCCGTTGGCGCCC
GATGCCGTCTGGAAATCTCACCGGAGCGATTCGCATTCGGGGCTGTGGATGCGCCCGTCTCACGCTGA

Upstream 100 bases:

>100_bases
GGCAATTTTGCTGACCGAAGAGGTCTCCTGACTGAAGTCGGCATAACAACATTAAAAACATCAGCAGTACTGAAACGTAC
TGCTGGTGTTTTTTGCTTTC

Downstream 100 bases:

>100_bases
AACCGGGCTTGCTGAAAAGCCTGCTGCACTATAAATTACCATTTACTTTTCAGGCAAACCGATACCCGTCATCATGATCC
GGTTGACCGCAAGCAACAAT

Product: muramoyltetrapeptide carboxypeptidase, putative

Products: NA

Alternate protein names: LD-carboxypeptidase [H]

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MNILIPKALRRGEVIGLISPSSTCAEPEKIERAVTYLERCGYKVKTSLYLNRSEHDPAHTDRYKLHDLHQMFADREVRAI
FCLRGGAGATRLLDRIDYGLIAANPKILVGYSDITALSLAVFRKTGLVNFSGPMAATELLAPSSYTEEHFWGMLTDPGYS
KHLTNFSEHPISCIRPGAVTGRLVGGNLSVLSSLVGTPYLPSFSGALLFTEDVNEPAYRIDRMLSHLFNAGLAQKCRGLM
FGQFSKNPADENRDYRFDKMFTYYANRMHDGVPVMTGLSYGHIRELMTLPVGARCRLEISPERFAFGAVDAPVSR

Sequences:

>Translated_315_residues
MNILIPKALRRGEVIGLISPSSTCAEPEKIERAVTYLERCGYKVKTSLYLNRSEHDPAHTDRYKLHDLHQMFADREVRAI
FCLRGGAGATRLLDRIDYGLIAANPKILVGYSDITALSLAVFRKTGLVNFSGPMAATELLAPSSYTEEHFWGMLTDPGYS
KHLTNFSEHPISCIRPGAVTGRLVGGNLSVLSSLVGTPYLPSFSGALLFTEDVNEPAYRIDRMLSHLFNAGLAQKCRGLM
FGQFSKNPADENRDYRFDKMFTYYANRMHDGVPVMTGLSYGHIRELMTLPVGARCRLEISPERFAFGAVDAPVSR
>Mature_315_residues
MNILIPKALRRGEVIGLISPSSTCAEPEKIERAVTYLERCGYKVKTSLYLNRSEHDPAHTDRYKLHDLHQMFADREVRAI
FCLRGGAGATRLLDRIDYGLIAANPKILVGYSDITALSLAVFRKTGLVNFSGPMAATELLAPSSYTEEHFWGMLTDPGYS
KHLTNFSEHPISCIRPGAVTGRLVGGNLSVLSSLVGTPYLPSFSGALLFTEDVNEPAYRIDRMLSHLFNAGLAQKCRGLM
FGQFSKNPADENRDYRFDKMFTYYANRMHDGVPVMTGLSYGHIRELMTLPVGARCRLEISPERFAFGAVDAPVSR

Specific function: May be involved in the degradation of peptidoglycan by catalyzing the cleavage of the terminal D-alanine residue from cytoplasmic murein peptides [H]

COG id: COG1619

COG function: function code V; Uncharacterized proteins, homologs of microcin C7 resistance protein MccF

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S66 family [H]

Homologues:

Organism=Escherichia coli, GI1787441, Length=294, Percent_Identity=31.6326530612245, Blast_Score=152, Evalue=3e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003507 [H]

Pfam domain/function: PF02016 Peptidase_S66 [H]

EC number: NA

Molecular weight: Translated: 35019; Mature: 35019

Theoretical pI: Translated: 8.55; Mature: 8.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILIPKALRRGEVIGLISPSSTCAEPEKIERAVTYLERCGYKVKTSLYLNRSEHDPAHT
CCCCCCHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCC
DRYKLHDLHQMFADREVRAIFCLRGGAGATRLLDRIDYGLIAANPKILVGYSDITALSLA
CCHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHCCCEEECCCEEEEEECHHHHHHHH
VFRKTGLVNFSGPMAATELLAPSSYTEEHFWGMLTDPGYSKHLTNFSEHPISCIRPGAVT
HHHHCCCEECCCCHHHHHHHCCCCCCHHHHCEEECCCCCHHHHCCCCCCCHHHCCCCCCE
GRLVGGNLSVLSSLVGTPYLPSFSGALLFTEDVNEPAYRIDRMLSHLFNAGLAQKCRGLM
EEEECCCHHHHHHHHCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHCC
FGQFSKNPADENRDYRFDKMFTYYANRMHDGVPVMTGLSYGHIRELMTLPVGARCRLEIS
CCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHCCCHHHHHHHHHCCCCCEEEEEEC
PERFAFGAVDAPVSR
CCCEEECCCCCCCCC
>Mature Secondary Structure
MNILIPKALRRGEVIGLISPSSTCAEPEKIERAVTYLERCGYKVKTSLYLNRSEHDPAHT
CCCCCCHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCC
DRYKLHDLHQMFADREVRAIFCLRGGAGATRLLDRIDYGLIAANPKILVGYSDITALSLA
CCHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHCCCEEECCCEEEEEECHHHHHHHH
VFRKTGLVNFSGPMAATELLAPSSYTEEHFWGMLTDPGYSKHLTNFSEHPISCIRPGAVT
HHHHCCCEECCCCHHHHHHHCCCCCCHHHHCEEECCCCCHHHHCCCCCCCHHHCCCCCCE
GRLVGGNLSVLSSLVGTPYLPSFSGALLFTEDVNEPAYRIDRMLSHLFNAGLAQKCRGLM
EEEECCCHHHHHHHHCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHCC
FGQFSKNPADENRDYRFDKMFTYYANRMHDGVPVMTGLSYGHIRELMTLPVGARCRLEIS
CCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHCCCHHHHHHHHHCCCCCEEEEEEC
PERFAFGAVDAPVSR
CCCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]