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The map label for this gene is pgk [H]

Identifier: 21675031

GI number: 21675031

Start: 2088304

End: 2089497

Strand: Reverse

Name: pgk [H]

Synonym: CT2222

Alternate gene names: 21675031

Gene position: 2089497-2088304 (Counterclockwise)

Preceding gene: 21675032

Following gene: 21675030

Centisome position: 96.96

GC content: 59.88

Gene sequence:

>1194_bases
ATGCAGAAAAAGACATTGTCTGACATATCGTTACAAGGAAAACGCGTCCTGATGCGAGTCGATTTCAACGTGCCGCTCGA
CCAGGACAGGAACATCACTGACGAGAAAAGGATCGTGGAGGCACTGCCTTCAATCCGCAAGGTCATCGACAATGGCGGCA
GGCTGATTCTGATGTCGCACCTCGGAAGACCGAAAGGCAAGGTCAACCCGGCATTCTCCCTTTCACCGGTCGCAAAGCGC
CTCTCCGAGCTGCTTGATTGCCCGGTGACGATGGCTGGTGACTGCATCGGCACCGAGGTGATGCAGCAGGTGCTCGCCTT
GCAGGACGGTGACGTGCTCATGCTCGAAAACCTCCGCTTCCACCCCGAAGAGGAGGCTAATGATCCTGACTTCGCCAGGG
AGCTGGCTTCGCTGGGCGAAATCTACGTCAACGACGCATTCGGTACGGCGCACCGCGCCCACGCCTCGACCGAGGGCATC
ACCCACTTCATGCAGACCGCCGTTGCGGGCTACCTCATCGAAAAGGAGCTGCGCTATCTCGGTACGGCGCTCAACGATGC
ACAGCGTCCGTTCGTGGCCATTCTCGGCGGCGCGAAGATTTCAGGCAAGATCGACGTGCTCGAAAGCCTCTTCGAGAAAG
TCGATACCGTTCTTGTCGGCGGCGCGATGGTCTTCACCTTCTTTAAGGCTCAGGGCCTCGACGTTGGCAACTCGCTTGTC
GAAGAAAACAAGCTCGAACTGGCTGTTTCCCTGCTTGAAAAAGCCAAAGCGAAAAACGTGCGCTTGCTTCTTCCGGAAGA
CGTGGTCGTGGCCGGAGAGATTTCCGCCGACGCGCCTTCGCGTGTCGAGCCGGTCAGCGCCATCTCCGCAGGCATGATTG
GTCTCGACATCGGCCCGGCAACCATCGAAACCTACCGCAAGGAAATTCTCGACGCGAAAACCGTGCTCTGGAATGGCCCG
ATGGGTGTGTTCGAGATCGACCAGTTCGCCAGGGGCACCTTCGCCGTCGCCCAGGCGCTTGCCGACGCAACCGCAGAGGG
CGCGATCACCATCATCGGCGGCGGCGACTCGGCGGCGGCCATCGCAAAGGCTGGCCTCTCCGACAAGGTGACGCACGTTT
CGACCGGCGGTGGCGCCAGCCTCGAATTCCTCGAAGGCAAGGAGCTGCCGGGCATCGCAGCCCTGAACGATTAG

Upstream 100 bases:

>100_bases
CACGCCTTGACGAATCGCTCCGGGTATACCGCGGATGCCGGATATTATGTATATTCACGCAGGCAAACACTTTTAGTTTC
ATCAACCAACTGACAGGGAA

Downstream 100 bases:

>100_bases
ACCCTGACGAGCATTGATCGTCACGCAAGCGATTTCCGAACCGTATGAACAATCATGGGCGCGTCTGAGAAACGGCTCGA
CACCGGTCAGCATGTTTTCC

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MQKKTLSDISLQGKRVLMRVDFNVPLDQDRNITDEKRIVEALPSIRKVIDNGGRLILMSHLGRPKGKVNPAFSLSPVAKR
LSELLDCPVTMAGDCIGTEVMQQVLALQDGDVLMLENLRFHPEEEANDPDFARELASLGEIYVNDAFGTAHRAHASTEGI
THFMQTAVAGYLIEKELRYLGTALNDAQRPFVAILGGAKISGKIDVLESLFEKVDTVLVGGAMVFTFFKAQGLDVGNSLV
EENKLELAVSLLEKAKAKNVRLLLPEDVVVAGEISADAPSRVEPVSAISAGMIGLDIGPATIETYRKEILDAKTVLWNGP
MGVFEIDQFARGTFAVAQALADATAEGAITIIGGGDSAAAIAKAGLSDKVTHVSTGGGASLEFLEGKELPGIAALND

Sequences:

>Translated_397_residues
MQKKTLSDISLQGKRVLMRVDFNVPLDQDRNITDEKRIVEALPSIRKVIDNGGRLILMSHLGRPKGKVNPAFSLSPVAKR
LSELLDCPVTMAGDCIGTEVMQQVLALQDGDVLMLENLRFHPEEEANDPDFARELASLGEIYVNDAFGTAHRAHASTEGI
THFMQTAVAGYLIEKELRYLGTALNDAQRPFVAILGGAKISGKIDVLESLFEKVDTVLVGGAMVFTFFKAQGLDVGNSLV
EENKLELAVSLLEKAKAKNVRLLLPEDVVVAGEISADAPSRVEPVSAISAGMIGLDIGPATIETYRKEILDAKTVLWNGP
MGVFEIDQFARGTFAVAQALADATAEGAITIIGGGDSAAAIAKAGLSDKVTHVSTGGGASLEFLEGKELPGIAALND
>Mature_397_residues
MQKKTLSDISLQGKRVLMRVDFNVPLDQDRNITDEKRIVEALPSIRKVIDNGGRLILMSHLGRPKGKVNPAFSLSPVAKR
LSELLDCPVTMAGDCIGTEVMQQVLALQDGDVLMLENLRFHPEEEANDPDFARELASLGEIYVNDAFGTAHRAHASTEGI
THFMQTAVAGYLIEKELRYLGTALNDAQRPFVAILGGAKISGKIDVLESLFEKVDTVLVGGAMVFTFFKAQGLDVGNSLV
EENKLELAVSLLEKAKAKNVRLLLPEDVVVAGEISADAPSRVEPVSAISAGMIGLDIGPATIETYRKEILDAKTVLWNGP
MGVFEIDQFARGTFAVAQALADATAEGAITIIGGGDSAAAIAKAGLSDKVTHVSTGGGASLEFLEGKELPGIAALND

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family [H]

Homologues:

Organism=Homo sapiens, GI4505763, Length=415, Percent_Identity=45.5421686746988, Blast_Score=352, Evalue=5e-97,
Organism=Homo sapiens, GI31543397, Length=415, Percent_Identity=43.855421686747, Blast_Score=345, Evalue=3e-95,
Organism=Escherichia coli, GI1789294, Length=394, Percent_Identity=43.4010152284264, Blast_Score=300, Evalue=1e-82,
Organism=Caenorhabditis elegans, GI17508823, Length=414, Percent_Identity=47.1014492753623, Blast_Score=366, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6319857, Length=412, Percent_Identity=47.5728155339806, Blast_Score=364, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24581203, Length=417, Percent_Identity=47.0023980815348, Blast_Score=357, Evalue=6e-99,
Organism=Drosophila melanogaster, GI17136394, Length=413, Percent_Identity=46.0048426150121, Blast_Score=334, Evalue=7e-92,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824 [H]

Pfam domain/function: PF00162 PGK [H]

EC number: =2.7.2.3 [H]

Molecular weight: Translated: 42290; Mature: 42290

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKKTLSDISLQGKRVLMRVDFNVPLDQDRNITDEKRIVEALPSIRKVIDNGGRLILMSH
CCCCCCCHHCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEC
LGRPKGKVNPAFSLSPVAKRLSELLDCPVTMAGDCIGTEVMQQVLALQDGDVLMLENLRF
CCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHCCCCCEEEECCCCC
HPEEEANDPDFARELASLGEIYVNDAFGTAHRAHASTEGITHFMQTAVAGYLIEKELRYL
CCCCCCCCHHHHHHHHHHHHEEEECCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
GTALNDAQRPFVAILGGAKISGKIDVLESLFEKVDTVLVGGAMVFTFFKAQGLDVGNSLV
HHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
EENKLELAVSLLEKAKAKNVRLLLPEDVVVAGEISADAPSRVEPVSAISAGMIGLDIGPA
HHHHHHHHHHHHHHHCCCCEEEECCCCEEEEEECCCCCCCCCCHHHHHHCCEEEECCCHH
TIETYRKEILDAKTVLWNGPMGVFEIDQFARGTFAVAQALADATAEGAITIIGGGDSAAA
HHHHHHHHHHHHHHEEECCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHH
IAKAGLSDKVTHVSTGGGASLEFLEGKELPGIAALND
HHHCCCCCCCEEEECCCCCEEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MQKKTLSDISLQGKRVLMRVDFNVPLDQDRNITDEKRIVEALPSIRKVIDNGGRLILMSH
CCCCCCCHHCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEC
LGRPKGKVNPAFSLSPVAKRLSELLDCPVTMAGDCIGTEVMQQVLALQDGDVLMLENLRF
CCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHCCCCCEEEECCCCC
HPEEEANDPDFARELASLGEIYVNDAFGTAHRAHASTEGITHFMQTAVAGYLIEKELRYL
CCCCCCCCHHHHHHHHHHHHEEEECCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
GTALNDAQRPFVAILGGAKISGKIDVLESLFEKVDTVLVGGAMVFTFFKAQGLDVGNSLV
HHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
EENKLELAVSLLEKAKAKNVRLLLPEDVVVAGEISADAPSRVEPVSAISAGMIGLDIGPA
HHHHHHHHHHHHHHHCCCCEEEECCCCEEEEEECCCCCCCCCCHHHHHHCCEEEECCCHH
TIETYRKEILDAKTVLWNGPMGVFEIDQFARGTFAVAQALADATAEGAITIIGGGDSAAA
HHHHHHHHHHHHHHEEECCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHH
IAKAGLSDKVTHVSTGGGASLEFLEGKELPGIAALND
HHHCCCCCCCEEEECCCCCEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA