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The map label for this gene is hetI [H]

Identifier: 21675005

GI number: 21675005

Start: 2069523

End: 2070251

Strand: Direct

Name: hetI [H]

Synonym: CT2196

Alternate gene names: 21675005

Gene position: 2069523-2070251 (Clockwise)

Preceding gene: 21675004

Following gene: 21675006

Centisome position: 96.04

GC content: 54.05

Gene sequence:

>729_bases
ATGATGATTATTTCGAAAGAGGCCGTCACCCTCATCCATACCGACACGCACATCGCCGGAATTCCGGAAGAAAAGCTTTT
CGAAACGCTTACCGATGAGGAAAAAGAAAAAGCCGATCGATTTCGTTTCGACAACGACCGACATAACTTTCTTCTCCGCA
GAGGTCTTCTCCGGCTTCTGCTTGGCGAGACGCTCAGCATTGAACCGTCCCTGATCCGGTTCTCAAGCACGCAGGTCGGC
AAGCCGTTCATGACCTTCCCCGAAAACACTGGCCTCTACTTCAACCTCTCCCATTCAGGCCGTCAGATTGTCTACGCTTT
TTCAAAACACCCTGAAATGGGCGTTGACATCGAAAGGATACGAACAGTGGATGACATCGATCTGCTGGCCCGCAAATACT
TCTCCGCCGAGGAGTACGCGATCATCGTCAACCTGCCCAGTCGCGAAAAAAACAAGGCATTCATCCGGATATGGAGCATC
AAGGAAGCCCTGATCAAAGCCAGCGGCTGGCCGCTCGAACACGGCCTTGCCGCCTTCGATGTCGCCACGCAGTACCGCAT
GACTCGCTTCAAGATGCCATTCGGAGCCAACCGGAGCCTCACCTGCATCACGCCCGTGTTCGACTACATGTGCGGCTTCG
CCACCGCGCTGGCCATCCAGCTCGACAACAACGAACCGTTGAACCTTCGCCGCTACTCGCTGCAAAACGGTGAGTATATC
GAACTCTGA

Upstream 100 bases:

>100_bases
CACGCTCCGGGAACGATGCCAGTCAAAAAATCGTAGATTGAGCAAAACGGAACGAGGCGCGCGAAATGGAACCACGGCCG
TCCAGAACCTGAGACATGAA

Downstream 100 bases:

>100_bases
ACAGCTTATCGACTTCGTGCTCTCACAACTCTCCCGCGATCACCATCAGCAAAAAGGAACGTAAGAGAAAAAACATGGAG
CCTGTTCACAAACGAAGAGA

Product: EntD/Gsp/HetI/Sfp family protein

Products: adenosine 3',5'-bisphosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 242; Mature: 242

Protein sequence:

>242_residues
MMIISKEAVTLIHTDTHIAGIPEEKLFETLTDEEKEKADRFRFDNDRHNFLLRRGLLRLLLGETLSIEPSLIRFSSTQVG
KPFMTFPENTGLYFNLSHSGRQIVYAFSKHPEMGVDIERIRTVDDIDLLARKYFSAEEYAIIVNLPSREKNKAFIRIWSI
KEALIKASGWPLEHGLAAFDVATQYRMTRFKMPFGANRSLTCITPVFDYMCGFATALAIQLDNNEPLNLRRYSLQNGEYI
EL

Sequences:

>Translated_242_residues
MMIISKEAVTLIHTDTHIAGIPEEKLFETLTDEEKEKADRFRFDNDRHNFLLRRGLLRLLLGETLSIEPSLIRFSSTQVG
KPFMTFPENTGLYFNLSHSGRQIVYAFSKHPEMGVDIERIRTVDDIDLLARKYFSAEEYAIIVNLPSREKNKAFIRIWSI
KEALIKASGWPLEHGLAAFDVATQYRMTRFKMPFGANRSLTCITPVFDYMCGFATALAIQLDNNEPLNLRRYSLQNGEYI
EL
>Mature_242_residues
MMIISKEAVTLIHTDTHIAGIPEEKLFETLTDEEKEKADRFRFDNDRHNFLLRRGLLRLLLGETLSIEPSLIRFSSTQVG
KPFMTFPENTGLYFNLSHSGRQIVYAFSKHPEMGVDIERIRTVDDIDLLARKYFSAEEYAIIVNLPSREKNKAFIRIWSI
KEALIKASGWPLEHGLAAFDVATQYRMTRFKMPFGANRSLTCITPVFDYMCGFATALAIQLDNNEPLNLRRYSLQNGEYI
EL

Specific function: Probably activates the acyl carrier protein (ACP) domain of hetM, by transferring the 4'-phosphopantetheinyl moiety of coenzyme A (CoA) to a serine residue. May be required for maintaining vegetative growth and probably acts via hetN to inhibit differenti

COG id: COG2091

COG function: function code H; Phosphopantetheinyl transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the P-Pant transferase superfamily. Gsp/sfp/hetI/acpT family [H]

Homologues:

Organism=Escherichia coli, GI1789886, Length=112, Percent_Identity=39.2857142857143, Blast_Score=65, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17569611, Length=169, Percent_Identity=33.7278106508876, Blast_Score=77, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008278 [H]

Pfam domain/function: PF01648 ACPS [H]

EC number: NA

Molecular weight: Translated: 27919; Mature: 27919

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMIISKEAVTLIHTDTHIAGIPEEKLFETLTDEEKEKADRFRFDNDRHNFLLRRGLLRLL
CEEECCCCEEEEEECCEECCCCHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHH
LGETLSIEPSLIRFSSTQVGKPFMTFPENTGLYFNLSHSGRQIVYAFSKHPEMGVDIERI
HCCCCCCCCCEEEECCCCCCCCCEECCCCCCEEEEECCCCCEEEEEECCCCCCCCCHHHH
RTVDDIDLLARKYFSAEEYAIIVNLPSREKNKAFIRIWSIKEALIKASGWPLEHGLAAFD
CCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCEEEEEEEHHHHHHHCCCCCHHHCCHHHH
VATQYRMTRFKMPFGANRSLTCITPVFDYMCGFATALAIQLDNNEPLNLRRYSLQNGEYI
HHHHHHHHEEECCCCCCCCEEEHHHHHHHHHHHHEEEEEEECCCCCCEEEEEECCCCCEE
EL
EC
>Mature Secondary Structure
MMIISKEAVTLIHTDTHIAGIPEEKLFETLTDEEKEKADRFRFDNDRHNFLLRRGLLRLL
CEEECCCCEEEEEECCEECCCCHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHH
LGETLSIEPSLIRFSSTQVGKPFMTFPENTGLYFNLSHSGRQIVYAFSKHPEMGVDIERI
HCCCCCCCCCEEEECCCCCCCCCEECCCCCCEEEEECCCCCEEEEEECCCCCCCCCHHHH
RTVDDIDLLARKYFSAEEYAIIVNLPSREKNKAFIRIWSIKEALIKASGWPLEHGLAAFD
CCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCEEEEEEEHHHHHHHCCCCCHHHCCHHHH
VATQYRMTRFKMPFGANRSLTCITPVFDYMCGFATALAIQLDNNEPLNLRRYSLQNGEYI
HHHHHHHHEEECCCCCCCCEEEHHHHHHHHHHHHEEEEEEECCCCCCEEEEEECCCCCEE
EL
EC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Coenzyme A; apo-[acyl carrier protein] [C]

Specific reaction: Coenzyme A + apo-[acyl carrier protein] = adenosine 3',5'-bisphosphate + [acyl carrier protein] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8157596; 11759840; 8939709 [H]