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The map label for this gene is leuA-2 [H]

Identifier: 21674916

GI number: 21674916

Start: 1995735

End: 1997423

Strand: Reverse

Name: leuA-2 [H]

Synonym: CT2107

Alternate gene names: 21674916

Gene position: 1997423-1995735 (Counterclockwise)

Preceding gene: 21674919

Following gene: 21674915

Centisome position: 92.69

GC content: 60.57

Gene sequence:

>1689_bases
ATGAGTACGATGAATTATAAGAAATACGCACCCTATCCGACGGTTGGGCTGAAAGACAGAACCTGGCCGGACAAGACCAT
TACCAAAGCGCCGATCTGGTGCAGCGTCGATCTGCGCGATGGCAACCAGGCGCTTCCGATTCCGATGAGCGTCGATGAGA
AGGTGGAGTTTTTCAGGCTGCTGGTATCGGTTGGCTTCAAGGAGATCGAGGTGGGCTTCCCGTCGGCTTCGGCCACGGAG
TTCGCCTTTGTGCGCAAGCTGATCGAAAACAATCTCATTCCTGACGACGTGAGCATCCAGGTGTTGACGCAGTCGCGCGA
GCACCTCATCCGCCGCACCTTCGAGGCGATCAAGGGCGCGAAGAACGCCATCGTGCACCTCTACAACTCCACCTCGCGCC
AGCAGCGCGACATAGTGTTCCGCATGAGCCGCGAGGAGATCATCACCATCGCCGTCGCCGGAACGCGGCTGGTGCGCGAG
CTGAAGGAGGCGAGCGGCAATCCAGGCATCCGCTTCGAGTACAGCCCTGAAAGCTTCACTGGCACCGAGCTGGAGTACGC
GCTGGACGTGTGCCACGCGGTGATGGACGAGTGGGGCGCATCGGCCACCAACAAGGTGATCCTGAACCTGCCTTCGACGG
TCGAGCTTTCGACGCCGAACGTCTATGCCGACCGCATCGAGTGGTTTTGCCGCCACATCAAGAATCGCGACGCGGTGCTC
CTCAGTGTGCATGCCCATAACGACCGCGGCACGGCGGTGGCGACCTCCGAGCTGGCGGTGATGGCCGGGGCGGATCGCAT
CGAAGGTGCCCTGTTCGGCAACGGCGAGCGGTGTGGCAACATGGACATTATCATCATGGCGCTGAACCTGATGACGCAAG
GTGTCGATCCGGAGCTCGATTTCTCCAACCTGCCGCATATCAAGCAGGTCTACTCTCGCTGCACCCGCATGACGGTGCAT
CCGCGCCATCCCTACTCGGGCGATCTGGTCTACACCGCCTTTTCCGGTTCGCACCAGGACGCGATCAGCAAGGGAATGAA
AGCGCACCAGCGGGCTGCCGACGGCGTGTGGGACGTGCCCTATCTGCCGATCGATCCCGAGGATGTTGGTTGCAACTACG
AGGCCATCGTCCGGATCAACAGCCAGTCGGGCAAGGGAGGCATAGCCTATGTGCTCGAAAAGGAGTATGGTATTCAGATC
CCGAAGTGGATGCAGCCAGATTTCGGTGCGGTTGTTCAGGAGGTGACCGACCGCACCGGCGAGGAGCTGAGCGCCGAGCA
GATTCACGAGCTGTTCCAGAAGGAGTACATCGGCGCAACCGAGCCCTATCTGATGAAGAAGTGCACCATCAGCTGGAGCG
ACGAAGACCCGGATCGCAACGACGAGGTGGCGACGATCGTCAGCGCCTCGATGGTTGGGCCACAGGGCGAGTTCAGCTTC
AGGGCCAAAGGCAACGGCCCGCTGGACGCCTTCGTGCGCGGCATGGTGAGCCACACCGGCATCGATTTCCATGTCGACGA
ATACGCGGAGCACGCCATCGGCCACAGCTCGGACGCGATGGCCATCGCCTACATTCGCCTCTCGTTCGATGACGTGGCGC
TGGTGTGCGGTTCCGGTATCGACTCGAACATCAGCCTTGCCTCCATCAAGGCCATCGTCAGTGCGCTGAACCGCTACAAA
AAGGCTTGA

Upstream 100 bases:

>100_bases
TGCATGGAATTGAGATTCAAAGCCGCGATCCTCTCAGGGTAAGCGGCTTTTTTTCATTAAGGGACTTTTACTATCAAGTG
TTTTAAATGGAGACTCTCTC

Downstream 100 bases:

>100_bases
TCGTTTTTTTCGTCAGGCTTCCCGATGATGGGGAAGCCTGACCCTCTCCAACGTTTTGGATTCTCTGCTGTTATTCTTTT
TTTACAAAAGAGACACTTTT

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 562; Mature: 561

Protein sequence:

>562_residues
MSTMNYKKYAPYPTVGLKDRTWPDKTITKAPIWCSVDLRDGNQALPIPMSVDEKVEFFRLLVSVGFKEIEVGFPSASATE
FAFVRKLIENNLIPDDVSIQVLTQSREHLIRRTFEAIKGAKNAIVHLYNSTSRQQRDIVFRMSREEIITIAVAGTRLVRE
LKEASGNPGIRFEYSPESFTGTELEYALDVCHAVMDEWGASATNKVILNLPSTVELSTPNVYADRIEWFCRHIKNRDAVL
LSVHAHNDRGTAVATSELAVMAGADRIEGALFGNGERCGNMDIIIMALNLMTQGVDPELDFSNLPHIKQVYSRCTRMTVH
PRHPYSGDLVYTAFSGSHQDAISKGMKAHQRAADGVWDVPYLPIDPEDVGCNYEAIVRINSQSGKGGIAYVLEKEYGIQI
PKWMQPDFGAVVQEVTDRTGEELSAEQIHELFQKEYIGATEPYLMKKCTISWSDEDPDRNDEVATIVSASMVGPQGEFSF
RAKGNGPLDAFVRGMVSHTGIDFHVDEYAEHAIGHSSDAMAIAYIRLSFDDVALVCGSGIDSNISLASIKAIVSALNRYK
KA

Sequences:

>Translated_562_residues
MSTMNYKKYAPYPTVGLKDRTWPDKTITKAPIWCSVDLRDGNQALPIPMSVDEKVEFFRLLVSVGFKEIEVGFPSASATE
FAFVRKLIENNLIPDDVSIQVLTQSREHLIRRTFEAIKGAKNAIVHLYNSTSRQQRDIVFRMSREEIITIAVAGTRLVRE
LKEASGNPGIRFEYSPESFTGTELEYALDVCHAVMDEWGASATNKVILNLPSTVELSTPNVYADRIEWFCRHIKNRDAVL
LSVHAHNDRGTAVATSELAVMAGADRIEGALFGNGERCGNMDIIIMALNLMTQGVDPELDFSNLPHIKQVYSRCTRMTVH
PRHPYSGDLVYTAFSGSHQDAISKGMKAHQRAADGVWDVPYLPIDPEDVGCNYEAIVRINSQSGKGGIAYVLEKEYGIQI
PKWMQPDFGAVVQEVTDRTGEELSAEQIHELFQKEYIGATEPYLMKKCTISWSDEDPDRNDEVATIVSASMVGPQGEFSF
RAKGNGPLDAFVRGMVSHTGIDFHVDEYAEHAIGHSSDAMAIAYIRLSFDDVALVCGSGIDSNISLASIKAIVSALNRYK
KA
>Mature_561_residues
STMNYKKYAPYPTVGLKDRTWPDKTITKAPIWCSVDLRDGNQALPIPMSVDEKVEFFRLLVSVGFKEIEVGFPSASATEF
AFVRKLIENNLIPDDVSIQVLTQSREHLIRRTFEAIKGAKNAIVHLYNSTSRQQRDIVFRMSREEIITIAVAGTRLVREL
KEASGNPGIRFEYSPESFTGTELEYALDVCHAVMDEWGASATNKVILNLPSTVELSTPNVYADRIEWFCRHIKNRDAVLL
SVHAHNDRGTAVATSELAVMAGADRIEGALFGNGERCGNMDIIIMALNLMTQGVDPELDFSNLPHIKQVYSRCTRMTVHP
RHPYSGDLVYTAFSGSHQDAISKGMKAHQRAADGVWDVPYLPIDPEDVGCNYEAIVRINSQSGKGGIAYVLEKEYGIQIP
KWMQPDFGAVVQEVTDRTGEELSAEQIHELFQKEYIGATEPYLMKKCTISWSDEDPDRNDEVATIVSASMVGPQGEFSFR
AKGNGPLDAFVRGMVSHTGIDFHVDEYAEHAIGHSSDAMAIAYIRLSFDDVALVCGSGIDSNISLASIKAIVSALNRYKK
A

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=542, Percent_Identity=25.4612546125461, Blast_Score=129, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6324225, Length=562, Percent_Identity=46.9750889679715, Blast_Score=519, Evalue=1e-148,
Organism=Saccharomyces cerevisiae, GI6324682, Length=569, Percent_Identity=45.8699472759227, Blast_Score=512, Evalue=1e-146,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005668
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 62371; Mature: 62240

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS00070 ALDEHYDE_DEHYDR_CYS ; PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTMNYKKYAPYPTVGLKDRTWPDKTITKAPIWCSVDLRDGNQALPIPMSVDEKVEFFRL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCEECCCCCCCHHHHHHHHH
LVSVGFKEIEVGFPSASATEFAFVRKLIENNLIPDDVSIQVLTQSREHLIRRTFEAIKGA
HHHCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHCC
KNAIVHLYNSTSRQQRDIVFRMSREEIITIAVAGTRLVRELKEASGNPGIRFEYSPESFT
CCEEEEEECCCCCHHHHHHEEECCCEEEEEEEHHHHHHHHHHHCCCCCCEEEEECCCCCC
GTELEYALDVCHAVMDEWGASATNKVILNLPSTVELSTPNVYADRIEWFCRHIKNRDAVL
CCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEECCCCHHHHHHHHHHHHHCCCCEEE
LSVHAHNDRGTAVATSELAVMAGADRIEGALFGNGERCGNMDIIIMALNLMTQGVDPELD
EEEEECCCCCCEEEECCEEEEECCHHHCEEEECCCCCCCCCEEEEEEHHHHHCCCCCCCC
FSNLPHIKQVYSRCTRMTVHPRHPYSGDLVYTAFSGSHQDAISKGMKAHQRAADGVWDVP
CCCCCHHHHHHHHHHCEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCC
YLPIDPEDVGCNYEAIVRINSQSGKGGIAYVLEKEYGIQIPKWMQPDFGAVVQEVTDRTG
CCCCCCHHCCCCEEEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCC
EELSAEQIHELFQKEYIGATEPYLMKKCTISWSDEDPDRNDEVATIVSASMVGPQGEFSF
CCCCHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCHHHEEEHHHCCCCCCEEE
RAKGNGPLDAFVRGMVSHTGIDFHVDEYAEHAIGHSSDAMAIAYIRLSFDDVALVCGSGI
EECCCCCHHHHHHHHHHHCCCCEEHHHHHHHHCCCCCCCEEEEEEEECCCCEEEEECCCC
DSNISLASIKAIVSALNRYKKA
CCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
STMNYKKYAPYPTVGLKDRTWPDKTITKAPIWCSVDLRDGNQALPIPMSVDEKVEFFRL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCEECCCCCCCHHHHHHHHH
LVSVGFKEIEVGFPSASATEFAFVRKLIENNLIPDDVSIQVLTQSREHLIRRTFEAIKGA
HHHCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHCC
KNAIVHLYNSTSRQQRDIVFRMSREEIITIAVAGTRLVRELKEASGNPGIRFEYSPESFT
CCEEEEEECCCCCHHHHHHEEECCCEEEEEEEHHHHHHHHHHHCCCCCCEEEEECCCCCC
GTELEYALDVCHAVMDEWGASATNKVILNLPSTVELSTPNVYADRIEWFCRHIKNRDAVL
CCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEECCCCHHHHHHHHHHHHHCCCCEEE
LSVHAHNDRGTAVATSELAVMAGADRIEGALFGNGERCGNMDIIIMALNLMTQGVDPELD
EEEEECCCCCCEEEECCEEEEECCHHHCEEEECCCCCCCCCEEEEEEHHHHHCCCCCCCC
FSNLPHIKQVYSRCTRMTVHPRHPYSGDLVYTAFSGSHQDAISKGMKAHQRAADGVWDVP
CCCCCHHHHHHHHHHCEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCC
YLPIDPEDVGCNYEAIVRINSQSGKGGIAYVLEKEYGIQIPKWMQPDFGAVVQEVTDRTG
CCCCCCHHCCCCEEEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCC
EELSAEQIHELFQKEYIGATEPYLMKKCTISWSDEDPDRNDEVATIVSASMVGPQGEFSF
CCCCHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCHHHEEEHHHCCCCCCEEE
RAKGNGPLDAFVRGMVSHTGIDFHVDEYAEHAIGHSSDAMAIAYIRLSFDDVALVCGSGI
EECCCCCHHHHHHHHHHHCCCCEEHHHHHHHHCCCCCCCEEEEEEEECCCCEEEEECCCC
DSNISLASIKAIVSALNRYKKA
CCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]