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The map label for this gene is aam1 [H]

Identifier: 21674899

GI number: 21674899

Start: 1981757

End: 1983769

Strand: Reverse

Name: aam1 [H]

Synonym: CT2089

Alternate gene names: 21674899

Gene position: 1983769-1981757 (Counterclockwise)

Preceding gene: 21674900

Following gene: 21674898

Centisome position: 92.06

GC content: 61.4

Gene sequence:

>2013_bases
ATGAAGCCAGAGTTTGTTCTGCCAGCTCCGATTCGTTTAGACCGCCCTTTTGACGGCCGCCGACGCGTTGTGATCGATCG
TGTATTTCCTGAAATCGATGGAGGCCGCTTTCCTGTCAAAAGAGTTGAGGGCGACCGGATGACCGTCGAGGCCGATATTT
TCACCGATGGGGCGGATACCATTGTCGCCGAGCTGCTGTACCGCCGGAAGGGCGAGGCGGCGTGGAGCGCGGTTCCGATG
ACACACATTGGCAACGACCGCTGGAAGGCGTCGTTCGAGGTGGGTGCGCCGGGCATGTGTGAATATACCGTGCAGGGCTG
GGTTGACCATTTCGAGACCTGGCGGAAGGGGCTGCAAAAGAAGCTCGATGCGGGGCAGGATGTGTCGCTTGACTTGCGCA
TCGGCGCGACCATCGTTGAGTTAGGCGCGGCGCGGGCGAAGGATGAGGATGCGGGCACGCTGCACCACTACGTCGGTTTG
CTCTCGGTTGGCGGCAGCGATGCCGCAGTCGAGGCGGCCTTGAGCGAGGGGCTTCTGGCGGCCATGCGGCGTTCGCCCGA
AAAAGCGATGGCGACGCTGTACGACAAGATTTTGCTGCTTCAGATCGACCAGAAAAAGGCCGGATTCAGCACCTGGTACG
AATTTTTCCCGCGCTCGTGGTCGGAGGAGCCGGGCAAGCACGGCACCTTCCGCGACTGCATAAAACTCCTGCCGCGCATT
GCACGAATGGGCTTCGACGTCATCTACCTGCCGCCGATCCACCCCATTGGCCTCACCAAGCGCAAGGGCAAGAACAACGC
GCTCGTTGCCGGGCCGGACGATCCGGGAAGCTGCTGGGCCATCGGCAGCGCTGACGGTGGCCACACCTCGGTGCATCCCG
AACTCGGCACGATGGAGGAGTTCGAGGCCTTCGTGCAGGAGGCCGAAGCGCAGGGGATTTCGGTGGCGCTCGACATTGCC
TTCCAGTGCTCGCCCGACCATCCCTGGGTGAAAGAGCATCCGCAGTGGTTCCGCTGGCGTCCCGACGGCACGGTGCAGTT
CGCCGAGAATCCGCCGAAGCGCTACGAGGACATCCTTCCCATCGACTTCGAGTCGGAGGACTGGCAGAACCTCTGGATCG
CGCTGCGCGAGGTATTTCTTTTCTGGATCGGCAAGGGCGTCAAGATTTTCCGCGTGGACAATCCGCACACCAAGGCGTTC
GGTTTCTGGGAATGGGCGCTCGGCTCGATCCGCGAGCAGTATCCCGAGACGATGTTCCTCGCCGAGGCGTTCACCCGCCC
GAAGCTGATGGCGCGGCTCGCGAAGGGCGGCTACACCCATTCCTACACCTACTTCACCTGGCGCAACACCAAGCACGAGT
TGCAAGAGTACCTCACCGAGCTGACCCAGACCGAGCTGCGCGAGTACATGCGCCCGAACTTCTGGCCCAACACGCCGGAC
ATTCTGCACGAGGAGTTGCAGGGTGGCAGCCGCGCACGCTTTATCATCCGCTTCGTGCTCGCCGCCACGCTCTCGTCGAA
CTACGGCATATACGGCCCGGCCTACGAACTGTGCGAGCACGTGCCGTATCCCGGCAAGGAGGAGTACCTCGATTCGGAGA
AGTACGAAATCAAGCAGTGGGATCTTGACCGCCCTGGTAACATCCGCTCCGAGATTGCGATGGTCAACCGCATTCGCCAC
AACAATCCCGCGCTCCAGCAGACCAGCGACATCACCTTCGTGAAGATCGAGGTGTCGCAAGGTCAGGAGCACGACCAGCT
CATGGGCTACGTCAAGTGCTCGCCCGATGGCGCGAACATCATCCTCACCGTGGTGACGCTCGACGACCGGAACACGCAGG
GCGGCTGGCTGCGCTTTCCGCTTGAAAAGTTCGGGCGCCCGCACACCGAGCGCTTCACGGTCGAAGACCTGATTTCGGGA
CGCACCTTTGAGTGGAACGGCGAGTGGAACTACGTCGAGCTGAATCCGCACCAGATGCCGGCACATATTTTCAGGGTCAA
TCTCCCATCATAA

Upstream 100 bases:

>100_bases
TTTCGGTATTGCTCAGCTTTATGATGATGGCCGAAGCCTGTTCCCGGATAGTGCTGCCGGTCTCCGGAGGCACGGTCTGA
AGTTCCACTCATTACAGCCT

Downstream 100 bases:

>100_bases
CCTAAAGCACCTTTTTTACCACCAATGCCTAAAAAACAAGCTGTAACAAGCTATCAGCCGGAGTCGCTCTGGTACAAGGA
TGCCATTATTTATGAACTAC

Product: alpha-amylase family protein

Products: D-Glucose 6-phosphate; D-Glucose [C]

Alternate protein names: NA

Number of amino acids: Translated: 670; Mature: 670

Protein sequence:

>670_residues
MKPEFVLPAPIRLDRPFDGRRRVVIDRVFPEIDGGRFPVKRVEGDRMTVEADIFTDGADTIVAELLYRRKGEAAWSAVPM
THIGNDRWKASFEVGAPGMCEYTVQGWVDHFETWRKGLQKKLDAGQDVSLDLRIGATIVELGAARAKDEDAGTLHHYVGL
LSVGGSDAAVEAALSEGLLAAMRRSPEKAMATLYDKILLLQIDQKKAGFSTWYEFFPRSWSEEPGKHGTFRDCIKLLPRI
ARMGFDVIYLPPIHPIGLTKRKGKNNALVAGPDDPGSCWAIGSADGGHTSVHPELGTMEEFEAFVQEAEAQGISVALDIA
FQCSPDHPWVKEHPQWFRWRPDGTVQFAENPPKRYEDILPIDFESEDWQNLWIALREVFLFWIGKGVKIFRVDNPHTKAF
GFWEWALGSIREQYPETMFLAEAFTRPKLMARLAKGGYTHSYTYFTWRNTKHELQEYLTELTQTELREYMRPNFWPNTPD
ILHEELQGGSRARFIIRFVLAATLSSNYGIYGPAYELCEHVPYPGKEEYLDSEKYEIKQWDLDRPGNIRSEIAMVNRIRH
NNPALQQTSDITFVKIEVSQGQEHDQLMGYVKCSPDGANIILTVVTLDDRNTQGGWLRFPLEKFGRPHTERFTVEDLISG
RTFEWNGEWNYVELNPHQMPAHIFRVNLPS

Sequences:

>Translated_670_residues
MKPEFVLPAPIRLDRPFDGRRRVVIDRVFPEIDGGRFPVKRVEGDRMTVEADIFTDGADTIVAELLYRRKGEAAWSAVPM
THIGNDRWKASFEVGAPGMCEYTVQGWVDHFETWRKGLQKKLDAGQDVSLDLRIGATIVELGAARAKDEDAGTLHHYVGL
LSVGGSDAAVEAALSEGLLAAMRRSPEKAMATLYDKILLLQIDQKKAGFSTWYEFFPRSWSEEPGKHGTFRDCIKLLPRI
ARMGFDVIYLPPIHPIGLTKRKGKNNALVAGPDDPGSCWAIGSADGGHTSVHPELGTMEEFEAFVQEAEAQGISVALDIA
FQCSPDHPWVKEHPQWFRWRPDGTVQFAENPPKRYEDILPIDFESEDWQNLWIALREVFLFWIGKGVKIFRVDNPHTKAF
GFWEWALGSIREQYPETMFLAEAFTRPKLMARLAKGGYTHSYTYFTWRNTKHELQEYLTELTQTELREYMRPNFWPNTPD
ILHEELQGGSRARFIIRFVLAATLSSNYGIYGPAYELCEHVPYPGKEEYLDSEKYEIKQWDLDRPGNIRSEIAMVNRIRH
NNPALQQTSDITFVKIEVSQGQEHDQLMGYVKCSPDGANIILTVVTLDDRNTQGGWLRFPLEKFGRPHTERFTVEDLISG
RTFEWNGEWNYVELNPHQMPAHIFRVNLPS
>Mature_670_residues
MKPEFVLPAPIRLDRPFDGRRRVVIDRVFPEIDGGRFPVKRVEGDRMTVEADIFTDGADTIVAELLYRRKGEAAWSAVPM
THIGNDRWKASFEVGAPGMCEYTVQGWVDHFETWRKGLQKKLDAGQDVSLDLRIGATIVELGAARAKDEDAGTLHHYVGL
LSVGGSDAAVEAALSEGLLAAMRRSPEKAMATLYDKILLLQIDQKKAGFSTWYEFFPRSWSEEPGKHGTFRDCIKLLPRI
ARMGFDVIYLPPIHPIGLTKRKGKNNALVAGPDDPGSCWAIGSADGGHTSVHPELGTMEEFEAFVQEAEAQGISVALDIA
FQCSPDHPWVKEHPQWFRWRPDGTVQFAENPPKRYEDILPIDFESEDWQNLWIALREVFLFWIGKGVKIFRVDNPHTKAF
GFWEWALGSIREQYPETMFLAEAFTRPKLMARLAKGGYTHSYTYFTWRNTKHELQEYLTELTQTELREYMRPNFWPNTPD
ILHEELQGGSRARFIIRFVLAATLSSNYGIYGPAYELCEHVPYPGKEEYLDSEKYEIKQWDLDRPGNIRSEIAMVNRIRH
NNPALQQTSDITFVKIEVSQGQEHDQLMGYVKCSPDGANIILTVVTLDDRNTQGGWLRFPLEKFGRPHTERFTVEDLISG
RTFEWNGEWNYVELNPHQMPAHIFRVNLPS

Specific function: Unknown

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family. GlgE subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021828
- InterPro:   IPR006047
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF11896 DUF3416 [H]

EC number: 3.2.1.93 [C]

Molecular weight: Translated: 76609; Mature: 76609

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPEFVLPAPIRLDRPFDGRRRVVIDRVFPEIDGGRFPVKRVEGDRMTVEADIFTDGADT
CCCCEECCCCCEECCCCCCCCEEEEEECCCCCCCCCCCCEEECCCEEEEEEEEECCCHHH
IVAELLYRRKGEAAWSAVPMTHIGNDRWKASFEVGAPGMCEYTVQGWVDHFETWRKGLQK
HHHHHHHHCCCCCHHHCCCHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
KLDAGQDVSLDLRIGATIVELGAARAKDEDAGTLHHYVGLLSVGGSDAAVEAALSEGLLA
HHCCCCCCEEEEEECCHHHHHCCCCCCCCCCCHHHHHHHHHEECCCHHHHHHHHHHHHHH
AMRRSPEKAMATLYDKILLLQIDQKKAGFSTWYEFFPRSWSEEPGKHGTFRDCIKLLPRI
HHHCCHHHHHHHHHHEEEEEEECCCCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
ARMGFDVIYLPPIHPIGLTKRKGKNNALVAGPDDPGSCWAIGSADGGHTSVHPELGTMEE
HHCCCCEEEECCCCCCCCCCCCCCCCCEEECCCCCCCEEEEECCCCCCCCCCCCCCCHHH
FEAFVQEAEAQGISVALDIAFQCSPDHPWVKEHPQWFRWRPDGTVQFAENPPKRYEDILP
HHHHHHHHHCCCCEEEEEEEEEECCCCCCHHCCCCCEEECCCCCEEECCCCCHHHHCCCC
IDFESEDWQNLWIALREVFLFWIGKGVKIFRVDNPHTKAFGFWEWALGSIREQYPETMFL
CCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEHHHHHHHHHHHHCCHHHHH
AEAFTRPKLMARLAKGGYTHSYTYFTWRNTKHELQEYLTELTQTELREYMRPNFWPNTPD
HHHHCCHHHHHHHHCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
ILHEELQGGSRARFIIRFVLAATLSSNYGIYGPAYELCEHVPYPGKEEYLDSEKYEIKQW
HHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCHHHHCCCCCCEEEEC
DLDRPGNIRSEIAMVNRIRHNNPALQQTSDITFVKIEVSQGQEHDQLMGYVKCSPDGANI
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCEEEEEEECCCCCEE
ILTVVTLDDRNTQGGWLRFPLEKFGRPHTERFTVEDLISGRTFEWNGEWNYVELNPHQMP
EEEEEEECCCCCCCCEEECCHHHCCCCCCCCEEHHHHHCCCEEEECCCEEEEEECCCCCC
AHIFRVNLPS
EEEEEEECCC
>Mature Secondary Structure
MKPEFVLPAPIRLDRPFDGRRRVVIDRVFPEIDGGRFPVKRVEGDRMTVEADIFTDGADT
CCCCEECCCCCEECCCCCCCCEEEEEECCCCCCCCCCCCEEECCCEEEEEEEEECCCHHH
IVAELLYRRKGEAAWSAVPMTHIGNDRWKASFEVGAPGMCEYTVQGWVDHFETWRKGLQK
HHHHHHHHCCCCCHHHCCCHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
KLDAGQDVSLDLRIGATIVELGAARAKDEDAGTLHHYVGLLSVGGSDAAVEAALSEGLLA
HHCCCCCCEEEEEECCHHHHHCCCCCCCCCCCHHHHHHHHHEECCCHHHHHHHHHHHHHH
AMRRSPEKAMATLYDKILLLQIDQKKAGFSTWYEFFPRSWSEEPGKHGTFRDCIKLLPRI
HHHCCHHHHHHHHHHEEEEEEECCCCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
ARMGFDVIYLPPIHPIGLTKRKGKNNALVAGPDDPGSCWAIGSADGGHTSVHPELGTMEE
HHCCCCEEEECCCCCCCCCCCCCCCCCEEECCCCCCCEEEEECCCCCCCCCCCCCCCHHH
FEAFVQEAEAQGISVALDIAFQCSPDHPWVKEHPQWFRWRPDGTVQFAENPPKRYEDILP
HHHHHHHHHCCCCEEEEEEEEEECCCCCCHHCCCCCEEECCCCCEEECCCCCHHHHCCCC
IDFESEDWQNLWIALREVFLFWIGKGVKIFRVDNPHTKAFGFWEWALGSIREQYPETMFL
CCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEHHHHHHHHHHHHCCHHHHH
AEAFTRPKLMARLAKGGYTHSYTYFTWRNTKHELQEYLTELTQTELREYMRPNFWPNTPD
HHHHCCHHHHHHHHCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
ILHEELQGGSRARFIIRFVLAATLSSNYGIYGPAYELCEHVPYPGKEEYLDSEKYEIKQW
HHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCHHHHCCCCCCEEEEC
DLDRPGNIRSEIAMVNRIRHNNPALQQTSDITFVKIEVSQGQEHDQLMGYVKCSPDGANI
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCEEEEEEECCCCCEE
ILTVVTLDDRNTQGGWLRFPLEKFGRPHTERFTVEDLISGRTFEWNGEWNYVELNPHQMP
EEEEEEECCCCCCCCEEECCHHHCCCCCCCCEEHHHHHCCCEEEECCCEEEEEECCCCCC
AHIFRVNLPS
EEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: H2O; alpha,alpha'-Trehalose 6-phosphate [C]

Specific reaction: H2O + alpha,alpha'-Trehalose 6-phosphate --> D-Glucose 6-phosphate + D-Glucose [C]

General reaction: O-Glycosyl bond hydrolysis [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10970853 [H]