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The map label for this gene is gdhA [H]

Identifier: 21674833

GI number: 21674833

Start: 1920231

End: 1921487

Strand: Direct

Name: gdhA [H]

Synonym: CT2023

Alternate gene names: 21674833

Gene position: 1920231-1921487 (Clockwise)

Preceding gene: 21674832

Following gene: 21674834

Centisome position: 89.11

GC content: 60.62

Gene sequence:

>1257_bases
ATGGCCGGACTTACCAAAGAAAATCCTTTCGACATCGCCCGCCGCCAGCTCGATGCTGCCGCTGGAATCATCGGTCTTGA
CGCCGAAGTGCTCGAACTGCTGCGCTGGCCGATGCGGGAAATGCATGTCACCATTCCGGTCAAAATGGACGACGGAGCGG
TACGCGCTTTTCACGGCTTCAGGGTGCAGTACAACGACGCACGTGGCCCCAACAAGGGGGGCATCCGCTTTCATCCCGAC
GAAACTATCGACACGGTCCGGGCGCTTGCAGCATGGATGACCTGGAAAACCGCCGTCATGGACATTCCGCTCGGCGGCGC
CAAAGGGGGCGTTATCTGCAATCCGAAAACGATGTCGCCCGGCGAACTCGAACGGCTGTCGCGCAGCTACATCCGGCAGG
TCGGCCGCCTGCTCGACCTCGAAAAGGATGTGCCTGCACCCGACGTTTACACCACGCCACAGATCATGGCCTGGATGGCT
GACGAATACTCTTTCATGCAGGGGCACAACGATTTCGGCGTCATTACCGGCAAACCGCTGGCGCTCGGCGGATCGCTCGG
GCGCGGCGACGCGACGGCGCGGGGAGGCATTATCTGCATCCGTGAAGCCGCGAAGATGCTCGGCATCAATCTGCGCGGGA
AACCCGCCGCCATCAACGGCTTCGGCAACGCCGGAGCCTTCGCCCACAAGCTCGCCGTCGAGCTGCTCGGCATGAAAGTG
GTGGCCGTCTCCGACTCGAAAGGCAGCATTTACAACCCGGATGGATTCGATCATCAAGCCCTCATGGAGTACAAAAAGCA
ACACGGATCGGTCGCCGATTTTCCCGGCTCCACGCCGCTGACCGACGCCGGACTGCTGGAACTGGATGTGACCGTGCTCA
TACCGGCGGCCCTTGAAGATGAAATTTCCTGCCGCAACGCGCGGAACATCCAGGCGAAAATCGTCGCCGAACTGGCCAAC
GGTCCGACCACCCCGGAAGCTGACAAAATTTTGCACGAGCGCGGCGTCTATCTCATTCCCGACCTGCTCTGCAACGCGGG
TGGCGTAACCGTCTCCTATTTCGAAATGGTACAGAACGCCAGTGGATGGTACTGGGAAGAGGAGGTCGTACATCGCCAGC
TCGAAAAGAAAATGGCCGCCGCAATCAAAGCCGTGCATCAGGCCGCCGTGCAGTACAGCGTCGATAACCGCACAGCAGCC
ATGATCGTGGCGATTCGCCGCGTGGCCGAGGCGATGAAGCTGCGTGGCTGGGTGTGA

Upstream 100 bases:

>100_bases
ATCCTGAAATTTTTTCAATCTCTCGAACACTCCCCTCATGCAGTCATCGAACGATCATCCCCAGCCAGCCACGGAGCCAT
TCGTGCTTGACAACTGCTCA

Downstream 100 bases:

>100_bases
GGCTCAGAATGCGAATTGCAAGTGCCGGACAGGCCGTCCGGCACTTTGCGTTCATGACCGGAATACCGCCGGTGGGGAGT
TTCACCCCGCCCTGAGAATT

Product: glutamate dehydrogenase

Products: NA

Alternate protein names: GDH [H]

Number of amino acids: Translated: 418; Mature: 417

Protein sequence:

>418_residues
MAGLTKENPFDIARRQLDAAAGIIGLDAEVLELLRWPMREMHVTIPVKMDDGAVRAFHGFRVQYNDARGPNKGGIRFHPD
ETIDTVRALAAWMTWKTAVMDIPLGGAKGGVICNPKTMSPGELERLSRSYIRQVGRLLDLEKDVPAPDVYTTPQIMAWMA
DEYSFMQGHNDFGVITGKPLALGGSLGRGDATARGGIICIREAAKMLGINLRGKPAAINGFGNAGAFAHKLAVELLGMKV
VAVSDSKGSIYNPDGFDHQALMEYKKQHGSVADFPGSTPLTDAGLLELDVTVLIPAALEDEISCRNARNIQAKIVAELAN
GPTTPEADKILHERGVYLIPDLLCNAGGVTVSYFEMVQNASGWYWEEEVVHRQLEKKMAAAIKAVHQAAVQYSVDNRTAA
MIVAIRRVAEAMKLRGWV

Sequences:

>Translated_418_residues
MAGLTKENPFDIARRQLDAAAGIIGLDAEVLELLRWPMREMHVTIPVKMDDGAVRAFHGFRVQYNDARGPNKGGIRFHPD
ETIDTVRALAAWMTWKTAVMDIPLGGAKGGVICNPKTMSPGELERLSRSYIRQVGRLLDLEKDVPAPDVYTTPQIMAWMA
DEYSFMQGHNDFGVITGKPLALGGSLGRGDATARGGIICIREAAKMLGINLRGKPAAINGFGNAGAFAHKLAVELLGMKV
VAVSDSKGSIYNPDGFDHQALMEYKKQHGSVADFPGSTPLTDAGLLELDVTVLIPAALEDEISCRNARNIQAKIVAELAN
GPTTPEADKILHERGVYLIPDLLCNAGGVTVSYFEMVQNASGWYWEEEVVHRQLEKKMAAAIKAVHQAAVQYSVDNRTAA
MIVAIRRVAEAMKLRGWV
>Mature_417_residues
AGLTKENPFDIARRQLDAAAGIIGLDAEVLELLRWPMREMHVTIPVKMDDGAVRAFHGFRVQYNDARGPNKGGIRFHPDE
TIDTVRALAAWMTWKTAVMDIPLGGAKGGVICNPKTMSPGELERLSRSYIRQVGRLLDLEKDVPAPDVYTTPQIMAWMAD
EYSFMQGHNDFGVITGKPLALGGSLGRGDATARGGIICIREAAKMLGINLRGKPAAINGFGNAGAFAHKLAVELLGMKVV
AVSDSKGSIYNPDGFDHQALMEYKKQHGSVADFPGSTPLTDAGLLELDVTVLIPAALEDEISCRNARNIQAKIVAELANG
PTTPEADKILHERGVYLIPDLLCNAGGVTVSYFEMVQNASGWYWEEEVVHRQLEKKMAAAIKAVHQAAVQYSVDNRTAAM
IVAIRRVAEAMKLRGWV

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=343, Percent_Identity=44.0233236151604, Blast_Score=278, Evalue=5e-75,
Organism=Homo sapiens, GI4885281, Length=343, Percent_Identity=43.731778425656, Blast_Score=273, Evalue=2e-73,
Organism=Escherichia coli, GI1788059, Length=404, Percent_Identity=32.4257425742574, Blast_Score=178, Evalue=6e-46,
Organism=Caenorhabditis elegans, GI17544676, Length=444, Percent_Identity=35.5855855855856, Blast_Score=246, Evalue=2e-65,
Organism=Saccharomyces cerevisiae, GI6319256, Length=430, Percent_Identity=30.2325581395349, Blast_Score=152, Evalue=9e-38,
Organism=Saccharomyces cerevisiae, GI6324951, Length=420, Percent_Identity=28.8095238095238, Blast_Score=144, Evalue=2e-35,
Organism=Drosophila melanogaster, GI45549226, Length=388, Percent_Identity=37.1134020618557, Blast_Score=246, Evalue=2e-65,
Organism=Drosophila melanogaster, GI45553475, Length=444, Percent_Identity=34.009009009009, Blast_Score=244, Evalue=1e-64,
Organism=Drosophila melanogaster, GI24649283, Length=343, Percent_Identity=37.6093294460641, Blast_Score=226, Evalue=3e-59,
Organism=Drosophila melanogaster, GI24649460, Length=325, Percent_Identity=37.8461538461538, Blast_Score=207, Evalue=9e-54,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.3 [H]

Molecular weight: Translated: 45443; Mature: 45312

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGLTKENPFDIARRQLDAAAGIIGLDAEVLELLRWPMREMHVTIPVKMDDGAVRAFHGF
CCCCCCCCHHHHHHHHHHHHHCEEECCHHHHHHHHCCHHHEEEEEEEEECCCCEEEEECE
RVQYNDARGPNKGGIRFHPDETIDTVRALAAWMTWKTAVMDIPLGGAKGGVICNPKTMSP
EEEECCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCCEEECCCCCCC
GELERLSRSYIRQVGRLLDLEKDVPAPDVYTTPQIMAWMADEYSFMQGHNDFGVITGKPL
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCE
ALGGSLGRGDATARGGIICIREAAKMLGINLRGKPAAINGFGNAGAFAHKLAVELLGMKV
EECCCCCCCCCCCCCCEEEHHHHHHHHCCCCCCCCCEECCCCCCHHHHHHHHHHHHCEEE
VAVSDSKGSIYNPDGFDHQALMEYKKQHGSVADFPGSTPLTDAGLLELDVTVLIPAALED
EEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEEEECCHHCC
EISCRNARNIQAKIVAELANGPTTPEADKILHERGVYLIPDLLCNAGGVTVSYFEMVQNA
CHHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHCCCEEEHHHHHCCCCCHHHHHHHHHCC
SGWYWEEEVVHRQLEKKMAAAIKAVHQAAVQYSVDNRTAAMIVAIRRVAEAMKLRGWV
CCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AGLTKENPFDIARRQLDAAAGIIGLDAEVLELLRWPMREMHVTIPVKMDDGAVRAFHGF
CCCCCCCHHHHHHHHHHHHHCEEECCHHHHHHHHCCHHHEEEEEEEEECCCCEEEEECE
RVQYNDARGPNKGGIRFHPDETIDTVRALAAWMTWKTAVMDIPLGGAKGGVICNPKTMSP
EEEECCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCCEEECCCCCCC
GELERLSRSYIRQVGRLLDLEKDVPAPDVYTTPQIMAWMADEYSFMQGHNDFGVITGKPL
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCE
ALGGSLGRGDATARGGIICIREAAKMLGINLRGKPAAINGFGNAGAFAHKLAVELLGMKV
EECCCCCCCCCCCCCCEEEHHHHHHHHCCCCCCCCCEECCCCCCHHHHHHHHHHHHCEEE
VAVSDSKGSIYNPDGFDHQALMEYKKQHGSVADFPGSTPLTDAGLLELDVTVLIPAALED
EEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEEEECCHHCC
EISCRNARNIQAKIVAELANGPTTPEADKILHERGVYLIPDLLCNAGGVTVSYFEMVQNA
CHHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHCCCEEEHHHHHCCCCCHHHHHHHHHCC
SGWYWEEEVVHRQLEKKMAAAIKAVHQAAVQYSVDNRTAAMIVAIRRVAEAMKLRGWV
CCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8135516 [H]