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The map label for this gene is miaB

Identifier: 21674804

GI number: 21674804

Start: 1890043

End: 1891377

Strand: Reverse

Name: miaB

Synonym: CT1993

Alternate gene names: 21674804

Gene position: 1891377-1890043 (Counterclockwise)

Preceding gene: 21674805

Following gene: 21674803

Centisome position: 87.77

GC content: 60.75

Gene sequence:

>1335_bases
ATGCGTCAACGAATGAGCCGTATGCCATCATCATTTTACATTCACACCTTCGGCTGTCAGATGAACCAGGCGGACTCGGA
GATCGTCACGGCGCTGCTTCGGGCCGAGGGGTTTGTTCCATCGGCAGATGAGACGAACGCCGATATCGTGCTGCTCAACT
CCTGTGCGGTGCGTGAGAATGCCGAGGAGCGGCTCGGCAACATTCTCATGCACCTCAAAGGGCGCAAGCGGCGGTGCAAG
GAGCTGGTGATCGGTGTGCTTGGATGCGTGCCGCAGTTCGAACGTGAACGGGTGTTCAGCGACTATCCCTTTGTCGATTT
CATCGTCGGGCCAGACAACTATCGCGAGCTTGCCGGTCTCGTCGCAGGCTTGCGCGAGGCGGTGGCGCGCCCCGCTCTTC
TTGACTACGACCAGACGGAGACCTACGCGGGCATCGAGCCGGTTCGCGCCGGGTCGATCAGCGCGTTCCTGCCGGTGATG
CGCGGCTGCAACAACCACTGCGCCTTCTGCGTGGTGCCAGTGACGCGCGGGCGCGAGCGCAGCGTCGGTTTCGAGCGCGT
GGTGGCTGAGGTGGTTGCACTGGAAAAAGCCGGATTCCGCGAGGTGACGCTGCTCGGCCAGAACGTCAACTCATGGCGCG
ACGCCGAAAAGGGGCTCGACTTCGCCGGATTGCTCGAAGGCGTGAGCCTCGCGGTGCCTTCAATGCGCATCCGTTTCACC
ACTTCGCATCCGAAGGACATCTCCGAAGCGCTCGTCAAGGTCATCGCTGCGCGCCCGAATCTCTGCAACCATATCCATTT
GCCCGTGCAGTCAGGATCGTCGCGAATGCTCGACCTGATGAAGCGTGGCCATACCCGTGAGGAGTATCTCGACCGGATCG
CCATGATCCGCAGCTACATTCCGGAGGTGGCCATTACCACCGATCTGATTGCCGGATTCTGCACCGAAACCGAAAAGGAT
CATCGCGAGACTCTTTCGCTGATGGAGGCAGTCGGTTACGATACGGCATTCATGTTCCACTATTCGGTACGCCCGGGCAC
CTGGGCGGCGCGTAACCTGCCGGACGATGTGCCGGACACGGTCAAAAAGGCGAGGCTTCAGGAGATCATCGAGCTGCAAA
ATGCCATATCGCGCGAGATTTTCCAGCGCGAGATCGGCAAGACGGTCGAGGTGCTTGCCGAAGCCGAGAGCAAGCGATCG
GAGTCCATGCTCATGGGCCGTACCCCCGAAAACCGTGTGGTGGTTTTCAGCCGGGGCAGGTTCAATCCCAGCGACACGCT
GCTGGTGAAAATCACGGGCGCCACCTCGGCTACGCTCTCCGGAGAGGCGGTTTAG

Upstream 100 bases:

>100_bases
AAGGAGGCGGCAGGGCGCGCCTTGTGACGACTCCGGTTCGCCGGACAAATCACGAGATCGCGCCTCGCTCCGTTCTGGTA
GTTGAGATCGAGCAAGCACT

Downstream 100 bases:

>100_bases
CGCCCGGTCACCCAACGGGCGGGCACTTCCTTCATAATTGTTCCCGATATGATGTTATTATTTCTGCGGGATTTGTAAAT
TTACGCAACATATAGAGGAG

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MRQRMSRMPSSFYIHTFGCQMNQADSEIVTALLRAEGFVPSADETNADIVLLNSCAVRENAEERLGNILMHLKGRKRRCK
ELVIGVLGCVPQFERERVFSDYPFVDFIVGPDNYRELAGLVAGLREAVARPALLDYDQTETYAGIEPVRAGSISAFLPVM
RGCNNHCAFCVVPVTRGRERSVGFERVVAEVVALEKAGFREVTLLGQNVNSWRDAEKGLDFAGLLEGVSLAVPSMRIRFT
TSHPKDISEALVKVIAARPNLCNHIHLPVQSGSSRMLDLMKRGHTREEYLDRIAMIRSYIPEVAITTDLIAGFCTETEKD
HRETLSLMEAVGYDTAFMFHYSVRPGTWAARNLPDDVPDTVKKARLQEIIELQNAISREIFQREIGKTVEVLAEAESKRS
ESMLMGRTPENRVVVFSRGRFNPSDTLLVKITGATSATLSGEAV

Sequences:

>Translated_444_residues
MRQRMSRMPSSFYIHTFGCQMNQADSEIVTALLRAEGFVPSADETNADIVLLNSCAVRENAEERLGNILMHLKGRKRRCK
ELVIGVLGCVPQFERERVFSDYPFVDFIVGPDNYRELAGLVAGLREAVARPALLDYDQTETYAGIEPVRAGSISAFLPVM
RGCNNHCAFCVVPVTRGRERSVGFERVVAEVVALEKAGFREVTLLGQNVNSWRDAEKGLDFAGLLEGVSLAVPSMRIRFT
TSHPKDISEALVKVIAARPNLCNHIHLPVQSGSSRMLDLMKRGHTREEYLDRIAMIRSYIPEVAITTDLIAGFCTETEKD
HRETLSLMEAVGYDTAFMFHYSVRPGTWAARNLPDDVPDTVKKARLQEIIELQNAISREIFQREIGKTVEVLAEAESKRS
ESMLMGRTPENRVVVFSRGRFNPSDTLLVKITGATSATLSGEAV
>Mature_444_residues
MRQRMSRMPSSFYIHTFGCQMNQADSEIVTALLRAEGFVPSADETNADIVLLNSCAVRENAEERLGNILMHLKGRKRRCK
ELVIGVLGCVPQFERERVFSDYPFVDFIVGPDNYRELAGLVAGLREAVARPALLDYDQTETYAGIEPVRAGSISAFLPVM
RGCNNHCAFCVVPVTRGRERSVGFERVVAEVVALEKAGFREVTLLGQNVNSWRDAEKGLDFAGLLEGVSLAVPSMRIRFT
TSHPKDISEALVKVIAARPNLCNHIHLPVQSGSSRMLDLMKRGHTREEYLDRIAMIRSYIPEVAITTDLIAGFCTETEKD
HRETLSLMEAVGYDTAFMFHYSVRPGTWAARNLPDDVPDTVKKARLQEIIELQNAISREIFQREIGKTVEVLAEAESKRS
ESMLMGRTPENRVVVFSRGRFNPSDTLLVKITGATSATLSGEAV

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=474, Percent_Identity=41.9831223628692, Blast_Score=358, Evalue=8e-99,
Organism=Homo sapiens, GI28872782, Length=474, Percent_Identity=41.9831223628692, Blast_Score=357, Evalue=1e-98,
Organism=Homo sapiens, GI93277076, Length=400, Percent_Identity=26.25, Blast_Score=134, Evalue=2e-31,
Organism=Escherichia coli, GI1786882, Length=442, Percent_Identity=38.0090497737557, Blast_Score=280, Evalue=1e-76,
Organism=Escherichia coli, GI1787057, Length=450, Percent_Identity=28, Blast_Score=147, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17553146, Length=463, Percent_Identity=40.8207343412527, Blast_Score=330, Evalue=6e-91,
Organism=Caenorhabditis elegans, GI71996771, Length=225, Percent_Identity=28.4444444444444, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21356207, Length=473, Percent_Identity=39.7463002114165, Blast_Score=341, Evalue=7e-94,
Organism=Drosophila melanogaster, GI19922432, Length=445, Percent_Identity=28.0898876404494, Blast_Score=132, Evalue=4e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_CHLTE (Q8KB05)

Other databases:

- EMBL:   AE006470
- RefSeq:   NP_662869.1
- ProteinModelPortal:   Q8KB05
- SMR:   Q8KB05
- GeneID:   1007568
- GenomeReviews:   AE006470_GR
- KEGG:   cte:CT1993
- NMPDR:   fig|194439.1.peg.1963
- TIGR:   CT1993
- HOGENOM:   HBG457663
- OMA:   EVRTYGC
- ProtClustDB:   PRK14340
- BioCyc:   CTEP194439:CT_1993-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 49472; Mature: 49472

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQRMSRMPSSFYIHTFGCQMNQADSEIVTALLRAEGFVPSADETNADIVLLNSCAVREN
CCCHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCHHHCC
AEERLGNILMHLKGRKRRCKELVIGVLGCVPQFERERVFSDYPFVDFIVGPDNYRELAGL
HHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCEEEEECCCCHHHHHHH
VAGLREAVARPALLDYDQTETYAGIEPVRAGSISAFLPVMRGCNNHCAFCVVPVTRGRER
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCC
SVGFERVVAEVVALEKAGFREVTLLGQNVNSWRDAEKGLDFAGLLEGVSLAVPSMRIRFT
CCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEE
TSHPKDISEALVKVIAARPNLCNHIHLPVQSGSSRMLDLMKRGHTREEYLDRIAMIRSYI
CCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHC
PEVAITTDLIAGFCTETEKDHRETLSLMEAVGYDTAFMFHYSVRPGTWAARNLPDDVPDT
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCHHH
VKKARLQEIIELQNAISREIFQREIGKTVEVLAEAESKRSESMLMGRTPENRVVVFSRGR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEEEECCC
FNPSDTLLVKITGATSATLSGEAV
CCCCCEEEEEEECCCCCCCCCCCC
>Mature Secondary Structure
MRQRMSRMPSSFYIHTFGCQMNQADSEIVTALLRAEGFVPSADETNADIVLLNSCAVREN
CCCHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCHHHCC
AEERLGNILMHLKGRKRRCKELVIGVLGCVPQFERERVFSDYPFVDFIVGPDNYRELAGL
HHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCEEEEECCCCHHHHHHH
VAGLREAVARPALLDYDQTETYAGIEPVRAGSISAFLPVMRGCNNHCAFCVVPVTRGRER
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCC
SVGFERVVAEVVALEKAGFREVTLLGQNVNSWRDAEKGLDFAGLLEGVSLAVPSMRIRFT
CCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEE
TSHPKDISEALVKVIAARPNLCNHIHLPVQSGSSRMLDLMKRGHTREEYLDRIAMIRSYI
CCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHC
PEVAITTDLIAGFCTETEKDHRETLSLMEAVGYDTAFMFHYSVRPGTWAARNLPDDVPDT
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCHHH
VKKARLQEIIELQNAISREIFQREIGKTVEVLAEAESKRSESMLMGRTPENRVVVFSRGR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEEEECCC
FNPSDTLLVKITGATSATLSGEAV
CCCCCEEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12093901