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The map label for this gene is dus1 [H]

Identifier: 21674741

GI number: 21674741

Start: 1823480

End: 1824538

Strand: Reverse

Name: dus1 [H]

Synonym: CT1929

Alternate gene names: 21674741

Gene position: 1824538-1823480 (Counterclockwise)

Preceding gene: 21674742

Following gene: 21674740

Centisome position: 84.67

GC content: 61.38

Gene sequence:

>1059_bases
ATGGAACAAAGCGCCATGCGCATCGGAACCATCGACATCGACCGCCCGATCATTCTCGCGCCCATGGAGGATGTGACCGA
TCGGGCCTTCCGGCAGCTCTGCAAGCAGCACGGGGCGGATATCGTCTATACCGAGTTCATCAGCGCGGAGGCACTGCGGC
GCGGCGTGGAGAAATCGATTCGCAAGATCACTGTGGCCGACCACGAACGGCCGGTTGCGGTGCAGATTTTCGGCAACACG
GTCGAGTCGATGGTCGAGGCGGCGGCGATTGCCGAGACGTACGAGCCGGATTACCTCGACATCAACTTTGGCTGTCCGGT
GAAAAAGGTTGCCGGTCGCGGCGCCGGAGCTGCGCTGCTGAAGGAGCCGGAAAAGCTTTCGGAGATCGCCTCGGCAGTGG
TCAACGCGGTCTCGCTGCCGGTGACGGCCAAGACCCGTATCGGCTGGGATCACGACTCGATCAACATCACCGACACTGTG
CGGCGGCTCGAAGATGCGGGTATCCGGGCGGTGGCGGTGCATGGGCGGACGCGCAGCGATATGTACAAGGGGCGGGCCGA
CTGGGGACGCATCGCCGAAGCGAAGCAGGCGTGCAGCATTCCGGTGATCGCTAATGGCGACGTCTGGCTGCCGGAAGACG
CGGTGGCCATGTTCGAGCGCACCGGTGCGGATGGCATCATGATCGGGCGCGGCTCGATCGGCAACCCCTTCATTTTCGAG
CAGGCGAAAAGTCTTGTCAAAGAGGGCGTGCGCCTTTCGGCTCCGTCCTATCGTCAGCGGATTCAGGCTGCTGTCGATCA
TCTTCGGCTTTCACTGGAGTACAAGGGCGAGAAATACGGTGTGCTTGAGATGCGCCGCCACTACTCGACTTATCTGAAAG
GACTTCCCGGTGTTTCGAAAGTGCGCAACCTGCTGGTGCGCGAACCCGATCCGGCGGTGATCGTCGAATTGCTCCGGGAG
TTCGAGGCTGCGTGCGAAGCGCTCGACCGCGAGGGGCTTCTGAAGGAGGGGGGTGAAAACCTGAACGATCACTCGCCCAG
ACTGATTCTCAATTACTAA

Upstream 100 bases:

>100_bases
GCGCGAGAACGTCAAGAAGCTGCTGAAGGAGGATGAGACCCTGCGCAATACGATCCGCCAGCAGGTTCGCGACATGCTCA
CCGGAGCGCCAACCGCATAA

Downstream 100 bases:

>100_bases
ACCATCATCCATTCTTACGTATATGAGCAGTGAAGCAGGCTATCGAGTAGCCGTTCTTGGCGCAACCGGCCTGGTTGGCA
GAACCATGATCAAGGTGCTC

Product: NifR3 family TIM-barrel protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MEQSAMRIGTIDIDRPIILAPMEDVTDRAFRQLCKQHGADIVYTEFISAEALRRGVEKSIRKITVADHERPVAVQIFGNT
VESMVEAAAIAETYEPDYLDINFGCPVKKVAGRGAGAALLKEPEKLSEIASAVVNAVSLPVTAKTRIGWDHDSINITDTV
RRLEDAGIRAVAVHGRTRSDMYKGRADWGRIAEAKQACSIPVIANGDVWLPEDAVAMFERTGADGIMIGRGSIGNPFIFE
QAKSLVKEGVRLSAPSYRQRIQAAVDHLRLSLEYKGEKYGVLEMRRHYSTYLKGLPGVSKVRNLLVREPDPAVIVELLRE
FEAACEALDREGLLKEGGENLNDHSPRLILNY

Sequences:

>Translated_352_residues
MEQSAMRIGTIDIDRPIILAPMEDVTDRAFRQLCKQHGADIVYTEFISAEALRRGVEKSIRKITVADHERPVAVQIFGNT
VESMVEAAAIAETYEPDYLDINFGCPVKKVAGRGAGAALLKEPEKLSEIASAVVNAVSLPVTAKTRIGWDHDSINITDTV
RRLEDAGIRAVAVHGRTRSDMYKGRADWGRIAEAKQACSIPVIANGDVWLPEDAVAMFERTGADGIMIGRGSIGNPFIFE
QAKSLVKEGVRLSAPSYRQRIQAAVDHLRLSLEYKGEKYGVLEMRRHYSTYLKGLPGVSKVRNLLVREPDPAVIVELLRE
FEAACEALDREGLLKEGGENLNDHSPRLILNY
>Mature_352_residues
MEQSAMRIGTIDIDRPIILAPMEDVTDRAFRQLCKQHGADIVYTEFISAEALRRGVEKSIRKITVADHERPVAVQIFGNT
VESMVEAAAIAETYEPDYLDINFGCPVKKVAGRGAGAALLKEPEKLSEIASAVVNAVSLPVTAKTRIGWDHDSINITDTV
RRLEDAGIRAVAVHGRTRSDMYKGRADWGRIAEAKQACSIPVIANGDVWLPEDAVAMFERTGADGIMIGRGSIGNPFIFE
QAKSLVKEGVRLSAPSYRQRIQAAVDHLRLSLEYKGEKYGVLEMRRHYSTYLKGLPGVSKVRNLLVREPDPAVIVELLRE
FEAACEALDREGLLKEGGENLNDHSPRLILNY

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=298, Percent_Identity=30.5369127516779, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI239788462, Length=289, Percent_Identity=31.1418685121107, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI40807366, Length=231, Percent_Identity=35.0649350649351, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI31742496, Length=226, Percent_Identity=32.7433628318584, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI8923374, Length=289, Percent_Identity=31.4878892733564, Blast_Score=105, Evalue=4e-23,
Organism=Escherichia coli, GI1789660, Length=288, Percent_Identity=37.8472222222222, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI1788462, Length=233, Percent_Identity=35.1931330472103, Blast_Score=124, Evalue=8e-30,
Organism=Escherichia coli, GI145693211, Length=240, Percent_Identity=27.9166666666667, Blast_Score=81, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17507177, Length=235, Percent_Identity=34.8936170212766, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17543114, Length=247, Percent_Identity=28.3400809716599, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI25144369, Length=214, Percent_Identity=32.2429906542056, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17510279, Length=252, Percent_Identity=32.1428571428571, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323437, Length=232, Percent_Identity=32.3275862068966, Blast_Score=112, Evalue=8e-26,
Organism=Saccharomyces cerevisiae, GI6323560, Length=306, Percent_Identity=29.0849673202614, Blast_Score=107, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6323433, Length=311, Percent_Identity=24.4372990353698, Blast_Score=93, Evalue=8e-20,
Organism=Drosophila melanogaster, GI24580595, Length=229, Percent_Identity=34.061135371179, Blast_Score=131, Evalue=9e-31,
Organism=Drosophila melanogaster, GI19920448, Length=229, Percent_Identity=34.061135371179, Blast_Score=131, Evalue=9e-31,
Organism=Drosophila melanogaster, GI24585320, Length=284, Percent_Identity=32.0422535211268, Blast_Score=130, Evalue=9e-31,
Organism=Drosophila melanogaster, GI19921524, Length=226, Percent_Identity=27.8761061946903, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI45549423, Length=247, Percent_Identity=30.7692307692308, Blast_Score=95, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 38879; Mature: 38879

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQSAMRIGTIDIDRPIILAPMEDVTDRAFRQLCKQHGADIVYTEFISAEALRRGVEKSI
CCCCCEEEEEEECCCCEEECCHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHH
RKITVADHERPVAVQIFGNTVESMVEAAAIAETYEPDYLDINFGCPVKKVAGRGAGAALL
HEEEECCCCCCEEEEEECHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHCCCCCHHHH
KEPEKLSEIASAVVNAVSLPVTAKTRIGWDHDSINITDTVRRLEDAGIRAVAVHGRTRSD
CCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEECCCCHHH
MYKGRADWGRIAEAKQACSIPVIANGDVWLPEDAVAMFERTGADGIMIGRGSIGNPFIFE
HHCCCCCHHHHHHHHHHCCCCEEECCCEECCHHHHHHHHHCCCCEEEEECCCCCCCHHHH
QAKSLVKEGVRLSAPSYRQRIQAAVDHLRLSLEYKGEKYGVLEMRRHYSTYLKGLPGVSK
HHHHHHHCCCCCCCHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHCCCCHHH
VRNLLVREPDPAVIVELLREFEAACEALDREGLLKEGGENLNDHSPRLILNY
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCEEEEEC
>Mature Secondary Structure
MEQSAMRIGTIDIDRPIILAPMEDVTDRAFRQLCKQHGADIVYTEFISAEALRRGVEKSI
CCCCCEEEEEEECCCCEEECCHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHH
RKITVADHERPVAVQIFGNTVESMVEAAAIAETYEPDYLDINFGCPVKKVAGRGAGAALL
HEEEECCCCCCEEEEEECHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHCCCCCHHHH
KEPEKLSEIASAVVNAVSLPVTAKTRIGWDHDSINITDTVRRLEDAGIRAVAVHGRTRSD
CCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEECCCCHHH
MYKGRADWGRIAEAKQACSIPVIANGDVWLPEDAVAMFERTGADGIMIGRGSIGNPFIFE
HHCCCCCHHHHHHHHHHCCCCEEECCCEECCHHHHHHHHHCCCCEEEEECCCCCCCHHHH
QAKSLVKEGVRLSAPSYRQRIQAAVDHLRLSLEYKGEKYGVLEMRRHYSTYLKGLPGVSK
HHHHHHHCCCCCCCHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHCCCCHHH
VRNLLVREPDPAVIVELLREFEAACEALDREGLLKEGGENLNDHSPRLILNY
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]