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The map label for this gene is aroA

Identifier: 21674730

GI number: 21674730

Start: 1813535

End: 1814839

Strand: Reverse

Name: aroA

Synonym: CT1918

Alternate gene names: 21674730

Gene position: 1814839-1813535 (Counterclockwise)

Preceding gene: 21674731

Following gene: 21674729

Centisome position: 84.22

GC content: 64.67

Gene sequence:

>1305_bases
ATGAGCACTTTTCAGGGCGAGGTGACGACCCTTCCGCCGGACAAATCGATTTCGCATCGCGCCGCGCTTATCGGCGCGCT
CGCCGAAGGCACGACCGAGATTTCCAACTTTTCGGGTGGATACGATAACCAGTCAACCCTGTCGGTGCTGCGCGATGCCG
GTATTTCCATTCGACAGGAGGAGCTTTCCGCCGGTGACGGGCGGATCGAGCGGCGCGTGGTGATCGAGTCGAACGGCCTC
TGGAGTTTCCGTGAGCCCTCCGTGCCGCTGATGTGCAACAACTCCGGCAGTACGATGCGCATGATGGCGGGTATTATGGC
CGCGCAGCCTTTCCGGAGCGAGCTGGTGGGTGACGCTTCGCTGATGAAGCGCCCGATGAAGCGCGTCGCCGATCCGCTGC
GGCAGATGGGCGCCGAAATTTCGCTTTCCGACGCCGGAACCGCGCCGGTCGTCATCCACGGCACCAAAGCGCTGAAGACG
ATCGAGTACCGTCTGCCGGTGCCCTCGGCGCAGGTGAAGTCGCTCGTGGCCTTTGCGGCGCTGCACGCCGATGGCCAGTC
GAAAATCATCGAGCCGATCCGTTCGCGCGACCATACCGAGCTGATGCTGGGCCTCGCAACCATCGATCGGCCCGACGGCG
TTCGCGAGATCATCATCGACGGGCGCAAGCCGATTGCCGCCAAACCGTTCAAGGTACCCGCCGATCCCTCGGCGGCCTGC
TTCATGATCGCCCTCGGCCTGCTCGGCGAGCGCTCGGAGATCGTGCTGCGCAACGTCTGCCTCAATCCGACGCGCGTGGC
CTACATCGACGTGTTGCAGGAGGCGGGCGCGGGCCTCGGCATCGAGAACGTGCGTTCGGAGGGCGGCGAGCCGGTGGGTG
ATATCGTCGTCCGGAGCTGCTCCGGCTTGAAGCCGCTGCGCATCAGCGACCACGGCGTTGTGGCTGGGGTGATCGACGAG
GTGCCGATGCTTGCCGTGCTCTCGGCCTTCGCCTCGGGCGAGTTCGAGCTGCACAACGCCGCCGAGCTGCGCACCAAGGA
GAGCGACCGCATCGACGCGCTGGTGGTGAATCTTCAGCGTCTCGGCTTCGAGTGCGAGCAGTACGCCGATGGCTTTGTGG
TGAAGGGACGGAAAACCGTCGCTTCAGAAGAGGTTGAGATCGAGTGCTTCGACGACCACCGCATCGCCATGAGCTTCACC
ATCGCCGCCGAAGCCGCCGGCGCATCGCTGCGCCTCTCGGATCGCGACGTGGCGGGCGTGTCGTTCCCGAATTTCTTCGC
CCTGATCGACTCGCTGCGGCAGTAG

Upstream 100 bases:

>100_bases
GCGCTAACGAAGAGCGCAACTCCTGATTTTTCAGGAATTCAGTATTGAAAAGCAGCTCCCTTGGGGCTGCTTTTTTTATC
GAACTCAAAAACAGCCAGCA

Downstream 100 bases:

>100_bases
GGGGGAGAAGGGTAGGGGCGGGAATTTTCCCTGGGAGCGCCGAGCGCCTGCTCGGCAATGGAGCTGGAGGCTCCGCATCA
TGCTTTTACGATTCTTTCGA

Product: 3-phosphoshikimate 1-carboxyvinyltransferase

Products: NA

Alternate protein names: 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS

Number of amino acids: Translated: 434; Mature: 433

Protein sequence:

>434_residues
MSTFQGEVTTLPPDKSISHRAALIGALAEGTTEISNFSGGYDNQSTLSVLRDAGISIRQEELSAGDGRIERRVVIESNGL
WSFREPSVPLMCNNSGSTMRMMAGIMAAQPFRSELVGDASLMKRPMKRVADPLRQMGAEISLSDAGTAPVVIHGTKALKT
IEYRLPVPSAQVKSLVAFAALHADGQSKIIEPIRSRDHTELMLGLATIDRPDGVREIIIDGRKPIAAKPFKVPADPSAAC
FMIALGLLGERSEIVLRNVCLNPTRVAYIDVLQEAGAGLGIENVRSEGGEPVGDIVVRSCSGLKPLRISDHGVVAGVIDE
VPMLAVLSAFASGEFELHNAAELRTKESDRIDALVVNLQRLGFECEQYADGFVVKGRKTVASEEVEIECFDDHRIAMSFT
IAAEAAGASLRLSDRDVAGVSFPNFFALIDSLRQ

Sequences:

>Translated_434_residues
MSTFQGEVTTLPPDKSISHRAALIGALAEGTTEISNFSGGYDNQSTLSVLRDAGISIRQEELSAGDGRIERRVVIESNGL
WSFREPSVPLMCNNSGSTMRMMAGIMAAQPFRSELVGDASLMKRPMKRVADPLRQMGAEISLSDAGTAPVVIHGTKALKT
IEYRLPVPSAQVKSLVAFAALHADGQSKIIEPIRSRDHTELMLGLATIDRPDGVREIIIDGRKPIAAKPFKVPADPSAAC
FMIALGLLGERSEIVLRNVCLNPTRVAYIDVLQEAGAGLGIENVRSEGGEPVGDIVVRSCSGLKPLRISDHGVVAGVIDE
VPMLAVLSAFASGEFELHNAAELRTKESDRIDALVVNLQRLGFECEQYADGFVVKGRKTVASEEVEIECFDDHRIAMSFT
IAAEAAGASLRLSDRDVAGVSFPNFFALIDSLRQ
>Mature_433_residues
STFQGEVTTLPPDKSISHRAALIGALAEGTTEISNFSGGYDNQSTLSVLRDAGISIRQEELSAGDGRIERRVVIESNGLW
SFREPSVPLMCNNSGSTMRMMAGIMAAQPFRSELVGDASLMKRPMKRVADPLRQMGAEISLSDAGTAPVVIHGTKALKTI
EYRLPVPSAQVKSLVAFAALHADGQSKIIEPIRSRDHTELMLGLATIDRPDGVREIIIDGRKPIAAKPFKVPADPSAACF
MIALGLLGERSEIVLRNVCLNPTRVAYIDVLQEAGAGLGIENVRSEGGEPVGDIVVRSCSGLKPLRISDHGVVAGVIDEV
PMLAVLSAFASGEFELHNAAELRTKESDRIDALVVNLQRLGFECEQYADGFVVKGRKTVASEEVEIECFDDHRIAMSFTI
AAEAAGASLRLSDRDVAGVSFPNFFALIDSLRQ

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; sixth step. [C]

COG id: COG0128

COG function: function code E; 5-enolpyruvylshikimate-3-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EPSP synthase family

Homologues:

Organism=Escherichia coli, GI1787137, Length=435, Percent_Identity=25.5172413793103, Blast_Score=83, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AROA_CHLTE (Q8KB71)

Other databases:

- EMBL:   AE006470
- RefSeq:   NP_662795.1
- ProteinModelPortal:   Q8KB71
- GeneID:   1007846
- GenomeReviews:   AE006470_GR
- KEGG:   cte:CT1918
- NMPDR:   fig|194439.1.peg.1889
- TIGR:   CT1918
- HOGENOM:   HBG646626
- OMA:   LSRNHTE
- ProtClustDB:   PRK02427
- BioCyc:   CTEP194439:CT_1918-MONOMER
- BRENDA:   2.5.1.19
- GO:   GO:0005737
- HAMAP:   MF_00210
- InterPro:   IPR001986
- InterPro:   IPR006264
- InterPro:   IPR023193
- InterPro:   IPR013792
- Gene3D:   G3DSA:3.65.10.10
- PIRSF:   PIRSF000505
- TIGRFAMs:   TIGR01356

Pfam domain/function: PF00275 EPSP_synthase; SSF55205 RNA3'_cycl/enolpyr_transf_A/B

EC number: =2.5.1.19

Molecular weight: Translated: 46627; Mature: 46496

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS00104 EPSP_SYNTHASE_1; PS00885 EPSP_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTFQGEVTTLPPDKSISHRAALIGALAEGTTEISNFSGGYDNQSTLSVLRDAGISIRQE
CCCCCCCEEECCCCCCCHHHHHHHHHHHCCCHHHHCCCCCCCCHHHHHHHHHCCCCEEHH
ELSAGDGRIERRVVIESNGLWSFREPSVPLMCNNSGSTMRMMAGIMAAQPFRSELVGDAS
HHCCCCCCEEEEEEEECCCEECCCCCCCCEEECCCCCHHHHHHHHHHCCCHHHHHCCCHH
LMKRPMKRVADPLRQMGAEISLSDAGTAPVVIHGTKALKTIEYRLPVPSAQVKSLVAFAA
HHHHHHHHHHHHHHHHCCEEEECCCCCCCEEEECCHHEEEEEEECCCCHHHHHHHHHHHH
LHADGQSKIIEPIRSRDHTELMLGLATIDRPDGVREIIIDGRKPIAAKPFKVPADPSAAC
HHCCCHHHHHHHHHCCCCHHEEEEHHHCCCCCCCHHHEECCCCCCCCCCEECCCCCCHHH
FMIALGLLGERSEIVLRNVCLNPTRVAYIDVLQEAGAGLGIENVRSEGGEPVGDIVVRSC
HHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHCCCCCHHHHHHHHC
SGLKPLRISDHGVVAGVIDEVPMLAVLSAFASGEFELHNAAELRTKESDRIDALVVNLQR
CCCCCEEECCCCEEEHHHHHHHHHHHHHHHHCCCEEECCHHHHCCCCCCCHHHHHHHHHH
LGFECEQYADGFVVKGRKTVASEEVEIECFDDHRIAMSFTIAAEAAGASLRLSDRDVAGV
HCCCHHHHCCCEEEECCCEECCCCEEEEEECCCEEEEEEEEEEECCCCEEEECCCCCCCC
SFPNFFALIDSLRQ
CCCHHHHHHHHHCC
>Mature Secondary Structure 
STFQGEVTTLPPDKSISHRAALIGALAEGTTEISNFSGGYDNQSTLSVLRDAGISIRQE
CCCCCCEEECCCCCCCHHHHHHHHHHHCCCHHHHCCCCCCCCHHHHHHHHHCCCCEEHH
ELSAGDGRIERRVVIESNGLWSFREPSVPLMCNNSGSTMRMMAGIMAAQPFRSELVGDAS
HHCCCCCCEEEEEEEECCCEECCCCCCCCEEECCCCCHHHHHHHHHHCCCHHHHHCCCHH
LMKRPMKRVADPLRQMGAEISLSDAGTAPVVIHGTKALKTIEYRLPVPSAQVKSLVAFAA
HHHHHHHHHHHHHHHHCCEEEECCCCCCCEEEECCHHEEEEEEECCCCHHHHHHHHHHHH
LHADGQSKIIEPIRSRDHTELMLGLATIDRPDGVREIIIDGRKPIAAKPFKVPADPSAAC
HHCCCHHHHHHHHHCCCCHHEEEEHHHCCCCCCCHHHEECCCCCCCCCCEECCCCCCHHH
FMIALGLLGERSEIVLRNVCLNPTRVAYIDVLQEAGAGLGIENVRSEGGEPVGDIVVRSC
HHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHCCCCCHHHHHHHHC
SGLKPLRISDHGVVAGVIDEVPMLAVLSAFASGEFELHNAAELRTKESDRIDALVVNLQR
CCCCCEEECCCCEEEHHHHHHHHHHHHHHHHCCCEEECCHHHHCCCCCCCHHHHHHHHHH
LGFECEQYADGFVVKGRKTVASEEVEIECFDDHRIAMSFTIAAEAAGASLRLSDRDVAGV
HCCCHHHHCCCEEEECCCEECCCCEEEEEECCCEEEEEEEEEEECCCCEEEECCCCCCCC
SFPNFFALIDSLRQ
CCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12093901