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The map label for this gene is hydG-1 [H]

Identifier: 21674704

GI number: 21674704

Start: 1791623

End: 1792447

Strand: Direct

Name: hydG-1 [H]

Synonym: CT1892

Alternate gene names: 21674704

Gene position: 1791623-1792447 (Clockwise)

Preceding gene: 21674703

Following gene: 21674705

Centisome position: 83.14

GC content: 60.61

Gene sequence:

>825_bases
ATGGTGACCGACCACGGCTACAAGTGCCGCATCACCAACATCGTGCCGCTATCGGAGCACGAAAAGCTCTTTCAGCTCCG
CATCGTCGATCCGCGCGAGCGCGAGCTGTTCACCTTCAGGCCGGGCCAGTTCCTGATGCTCGAAGTGCCGGGCTACGGCG
AAGCGCCGATCTCGATTTCGAGTGCCACGAGCAACCGCGAATTCATCGAGCTGTGCATCCGCAAGGCGGGCCACGTCACC
TCGGCGCTGTTCGAGGCAAAGCAAGGCGCGTTTGTCGCCGTTCGCGGGCCGTTCGGCACCTCGTTTCCGATGGAGGCAAT
GCAGGATCACGACGTGCTGCTCATCGCGGGCGGCCTCGGCATCGCCCCGTTGCGCGCGCCGCTCTTCTGGATCAACGACC
ACCGCGACCACTACCGCAACGTGAGCTTTCTGTACGGCGCAAAGGAGCCGTCGCAGATGCTCTTCACCTACCAGTTTGAG
GAGTGGAAGACCGTCAGCCACATCGACCTGCACACCATCGTCGAAAAACCGGATGACCAGTGGACGGGACGCACGGGAAT
GATTACCTTGCTCTTCGACGAGATCACGATCGACCCGAAGAACACCTGGGCGATTGTCTGCGGCCCGCCGGTGATGTTCA
AATTCGTCTGCACGCACCTCGACAAACTCGGCATTCCGATGAACCGGATGTTCGTGTCGCTGGAGCGCCACATGAATTGC
GGCATGGGCAAGTGCTGCCGCTGCATGGTCGGCTCGACCTTCACCTGCCTCGACGGCCCGGTATTCGACTACTGGAGCGT
CATGAATCTCAAGGAAGCGATTTAA

Upstream 100 bases:

>100_bases
AAGAATCATGCGTGACCTGCGTTTCACCATCACCGGCGAAGACCTGAACCGCGAGCCGTTCGCCCGCACGAGCCCCGATT
TCGGCAAGAAGTCGGAGGTA

Downstream 100 bases:

>100_bases
GGATTGACCACCATGAACCACCTCGGATTCGATAGAGAGAAAATCAGGATCGGCTCCTTCGACTTCACCTGCTGCGAAGG
GTGCCAGCTCCAGCTCGCCA

Product: hydrogenase/sulfur reductase, gamma subunit

Products: NAD(P); FMNH2; 2-octaprenyl-6-hydroxyphenol [C]

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MVTDHGYKCRITNIVPLSEHEKLFQLRIVDPRERELFTFRPGQFLMLEVPGYGEAPISISSATSNREFIELCIRKAGHVT
SALFEAKQGAFVAVRGPFGTSFPMEAMQDHDVLLIAGGLGIAPLRAPLFWINDHRDHYRNVSFLYGAKEPSQMLFTYQFE
EWKTVSHIDLHTIVEKPDDQWTGRTGMITLLFDEITIDPKNTWAIVCGPPVMFKFVCTHLDKLGIPMNRMFVSLERHMNC
GMGKCCRCMVGSTFTCLDGPVFDYWSVMNLKEAI

Sequences:

>Translated_274_residues
MVTDHGYKCRITNIVPLSEHEKLFQLRIVDPRERELFTFRPGQFLMLEVPGYGEAPISISSATSNREFIELCIRKAGHVT
SALFEAKQGAFVAVRGPFGTSFPMEAMQDHDVLLIAGGLGIAPLRAPLFWINDHRDHYRNVSFLYGAKEPSQMLFTYQFE
EWKTVSHIDLHTIVEKPDDQWTGRTGMITLLFDEITIDPKNTWAIVCGPPVMFKFVCTHLDKLGIPMNRMFVSLERHMNC
GMGKCCRCMVGSTFTCLDGPVFDYWSVMNLKEAI
>Mature_274_residues
MVTDHGYKCRITNIVPLSEHEKLFQLRIVDPRERELFTFRPGQFLMLEVPGYGEAPISISSATSNREFIELCIRKAGHVT
SALFEAKQGAFVAVRGPFGTSFPMEAMQDHDVLLIAGGLGIAPLRAPLFWINDHRDHYRNVSFLYGAKEPSQMLFTYQFE
EWKTVSHIDLHTIVEKPDDQWTGRTGMITLLFDEITIDPKNTWAIVCGPPVMFKFVCTHLDKLGIPMNRMFVSLERHMNC
GMGKCCRCMVGSTFTCLDGPVFDYWSVMNLKEAI

Specific function: This enzyme catalyzes the hydrogen sulfide production from sulfite. It is strictly anaerobic. It is regulated by electron acceptors rather than by cysteine [H]

COG id: COG0543

COG function: function code HC; 2-polyprenylphenol hydroxylase and related flavodoxin oxidoreductases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012165
- InterPro:   IPR019480
- InterPro:   IPR017927
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR017938
- InterPro:   IPR014260 [H]

Pfam domain/function: PF10418 DHODB_Fe-S_bind; PF00970 FAD_binding_6; PF00175 NAD_binding_1 [H]

EC number: 1.5.1.29 [C]

Molecular weight: Translated: 31192; Mature: 31192

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
8.0 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
8.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTDHGYKCRITNIVPLSEHEKLFQLRIVDPRERELFTFRPGQFLMLEVPGYGEAPISIS
CCCCCCCEEEEEEEECCCCCCEEEEEEEECCCCCCEEEECCCCEEEEECCCCCCCCEEEE
SATSNREFIELCIRKAGHVTSALFEAKQGAFVAVRGPFGTSFPMEAMQDHDVLLIAGGLG
CCCCCHHHHHHHHHHCCHHHHHHHHHCCCCEEEEECCCCCCCCHHHHCCCCEEEEECCCC
IAPLRAPLFWINDHRDHYRNVSFLYGAKEPSQMLFTYQFEEWKTVSHIDLHTIVEKPDDQ
CCCCCCCEEEEECCHHHHCCEEEEECCCCCCCEEEEEEECCCCEECEEEEEHHHCCCCCC
WTGRTGMITLLFDEITIDPKNTWAIVCGPPVMFKFVCTHLDKLGIPMNRMFVSLERHMNC
CCCCCCEEEEEEEEEEECCCCCEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCC
GMGKCCRCMVGSTFTCLDGPVFDYWSVMNLKEAI
CHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MVTDHGYKCRITNIVPLSEHEKLFQLRIVDPRERELFTFRPGQFLMLEVPGYGEAPISIS
CCCCCCCEEEEEEEECCCCCCEEEEEEEECCCCCCEEEECCCCEEEEECCCCCCCCEEEE
SATSNREFIELCIRKAGHVTSALFEAKQGAFVAVRGPFGTSFPMEAMQDHDVLLIAGGLG
CCCCCHHHHHHHHHHCCHHHHHHHHHCCCCEEEEECCCCCCCCHHHHCCCCEEEEECCCC
IAPLRAPLFWINDHRDHYRNVSFLYGAKEPSQMLFTYQFEEWKTVSHIDLHTIVEKPDDQ
CCCCCCCEEEEECCHHHHCCEEEEECCCCCCCEEEEEEECCCCEECEEEEEHHHCCCCCC
WTGRTGMITLLFDEITIDPKNTWAIVCGPPVMFKFVCTHLDKLGIPMNRMFVSLERHMNC
CCCCCCEEEEEEEEEEECCCCCEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCC
GMGKCCRCMVGSTFTCLDGPVFDYWSVMNLKEAI
CHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD(P)H2; FMN [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD(P)H2; FMN; 2 2-octaprenylphenol; O2 [C]

Specific reaction: NAD(P)H2 + FMN = NAD(P) + FMNH2 2 2-octaprenylphenol + O2 = 2 2-octaprenyl-6-hydroxyphenol [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1704886; 11677609 [H]