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The map label for this gene is menE [H]

Identifier: 21674661

GI number: 21674661

Start: 1747926

End: 1749302

Strand: Direct

Name: menE [H]

Synonym: CT1848

Alternate gene names: 21674661

Gene position: 1747926-1749302 (Clockwise)

Preceding gene: 21674660

Following gene: 21674666

Centisome position: 81.11

GC content: 64.27

Gene sequence:

>1377_bases
ATGGAGCTTGTAACCCAGGCCGCACAAACCTTCGGCGACCAACCCGCGCTCATCACCGACGAACGGCGCTGGAGCTTCGC
CGACCTCGATGGCGACACGGCTCGCATCGCCACTGCGTTCGAAGCGAGCAGCATCCGGCGCGGCGACATCGTTGCGCTTG
TCGCGCCGAACAGCCCGGCGCTCGTGCTCTCGCTGATGGCGCTCATGCGCATGGGCGCCGTCGCCGCGCCGGTGAACCAC
CGGTTTCCGGCAAATCACATCGAGGGCGTGCTCGCGCGGCTGAATCCGGCGATGACGCTCGACGCGGCAAAGCTCGACGC
CTTCGTCGCCGACGCGATCGCCCGGACGGGCGCGACCTTCACCGCCGCGACGGAGATGGAACGGCCCGTCTCGGTCATCC
ACACCTCGGCCAGCTCCGGCAAGCCGAAAGCCGCCGTGCACAGCCTCTCAAACCACTACCACAGCGCGATGGGTTCCGCG
CAAAACCTGCCCTTCGGGCCGGGCGACTGCTGGCTGCTTTCGCTCCCGCTCTACCACGTCGGCGGCTACTCGATGCTTTT
CAAATGCCTCCTCGGAGGCGGCGCGCTGGCCGTGCCGTCGCCTGATGCGGCGCTTGCTGAGTCTCTCACACACTTCCCCG
TCACGCACCTCTCGCTCGTGCCGACGCAGCTCTACCGGATGCTCCGCGCCGACGGCGGCCCGGAGCGCCTGCGAAGCCTC
AGGGCGTTATTGCTCGGAGGCAGCGCGGTCAGCGCCCCGCTTCTACGCGAGGCGATCTGCGAACGGGTACCGCTCTACCT
CACCTACGGCTCGACCGAAATGAGCACGCAGGTCACAACCTCGCCCACGCCAGTCACCAAGGCGCGGGGCGACAGCGGTG
TGGTGCTCCCTTACCGTGAGGTTGCAATTTCAGTGGACGGCGAAATTCTCGTCAAAGGCGAGTGCCTTTTCATGGGCTAT
CTCGATAATGGAGAATTGCGCGAGGCGCGAGACAAAAATGGATGGTTCCACACAGGCGACATGGGTGAACTGTCGGGGGA
CGCGCGGCTGACCGTACTCGGCAGGAAGGACAACATGTTCATCTCGGGCGGCGAGAACATCCACCCCGAAGAGATCGAAA
AAGCGCTGACATCGATCGTAGGCATCGAAGAGGCCGTGGTGGTTCCAGCGCCCGACGCCGAGTACGGCATGAGACCGGTA
GCGTGGATCAAAGCCCGGAGCGACTCGCCTGACGATGCCACAATCATCGCCAGCCTGAAAAGCACGATCGGCAAGCTCAA
AACACCGGTGGCGTTTCATCGCATTCAGGAGTGGCAGACCATCCCCGGCTCAGCCAAGATCGACCGAAGCTGGTACCGCA
AGCTGGCTGAAAAGTAA

Upstream 100 bases:

>100_bases
CCGCCACACCCTTCGCCACGCCGGACGGATTCGCCGATCCCGCAGCCGCATGGCGCGACAGCTTGCGCGTGAGACCCGAC
ATGATCGAAACCGTCAAGTC

Downstream 100 bases:

>100_bases
AAGGGCGACCAACCGGTCGCCCCTGCACATCACATGTCACCCGTCACCTCTTTCTTGCCTGCCGCTTCAGGAAAAACACC
GTCACACGACCGCCAAGACC

Product: O-succinylbenzoic acid-CoA ligase

Products: NA

Alternate protein names: o-succinylbenzoyl-CoA synthetase; OSB-CoA synthetase [H]

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MELVTQAAQTFGDQPALITDERRWSFADLDGDTARIATAFEASSIRRGDIVALVAPNSPALVLSLMALMRMGAVAAPVNH
RFPANHIEGVLARLNPAMTLDAAKLDAFVADAIARTGATFTAATEMERPVSVIHTSASSGKPKAAVHSLSNHYHSAMGSA
QNLPFGPGDCWLLSLPLYHVGGYSMLFKCLLGGGALAVPSPDAALAESLTHFPVTHLSLVPTQLYRMLRADGGPERLRSL
RALLLGGSAVSAPLLREAICERVPLYLTYGSTEMSTQVTTSPTPVTKARGDSGVVLPYREVAISVDGEILVKGECLFMGY
LDNGELREARDKNGWFHTGDMGELSGDARLTVLGRKDNMFISGGENIHPEEIEKALTSIVGIEEAVVVPAPDAEYGMRPV
AWIKARSDSPDDATIIASLKSTIGKLKTPVAFHRIQEWQTIPGSAKIDRSWYRKLAEK

Sequences:

>Translated_458_residues
MELVTQAAQTFGDQPALITDERRWSFADLDGDTARIATAFEASSIRRGDIVALVAPNSPALVLSLMALMRMGAVAAPVNH
RFPANHIEGVLARLNPAMTLDAAKLDAFVADAIARTGATFTAATEMERPVSVIHTSASSGKPKAAVHSLSNHYHSAMGSA
QNLPFGPGDCWLLSLPLYHVGGYSMLFKCLLGGGALAVPSPDAALAESLTHFPVTHLSLVPTQLYRMLRADGGPERLRSL
RALLLGGSAVSAPLLREAICERVPLYLTYGSTEMSTQVTTSPTPVTKARGDSGVVLPYREVAISVDGEILVKGECLFMGY
LDNGELREARDKNGWFHTGDMGELSGDARLTVLGRKDNMFISGGENIHPEEIEKALTSIVGIEEAVVVPAPDAEYGMRPV
AWIKARSDSPDDATIIASLKSTIGKLKTPVAFHRIQEWQTIPGSAKIDRSWYRKLAEK
>Mature_458_residues
MELVTQAAQTFGDQPALITDERRWSFADLDGDTARIATAFEASSIRRGDIVALVAPNSPALVLSLMALMRMGAVAAPVNH
RFPANHIEGVLARLNPAMTLDAAKLDAFVADAIARTGATFTAATEMERPVSVIHTSASSGKPKAAVHSLSNHYHSAMGSA
QNLPFGPGDCWLLSLPLYHVGGYSMLFKCLLGGGALAVPSPDAALAESLTHFPVTHLSLVPTQLYRMLRADGGPERLRSL
RALLLGGSAVSAPLLREAICERVPLYLTYGSTEMSTQVTTSPTPVTKARGDSGVVLPYREVAISVDGEILVKGECLFMGY
LDNGELREARDKNGWFHTGDMGELSGDARLTVLGRKDNMFISGGENIHPEEIEKALTSIVGIEEAVVVPAPDAEYGMRPV
AWIKARSDSPDDATIIASLKSTIGKLKTPVAFHRIQEWQTIPGSAKIDRSWYRKLAEK

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Homo sapiens, GI38505220, Length=467, Percent_Identity=25.6959314775161, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI156151445, Length=539, Percent_Identity=22.8200371057514, Blast_Score=106, Evalue=5e-23,
Organism=Homo sapiens, GI42544132, Length=496, Percent_Identity=25, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI122937307, Length=496, Percent_Identity=24.3951612903226, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI57165410, Length=150, Percent_Identity=33.3333333333333, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI57165412, Length=150, Percent_Identity=33.3333333333333, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI40807491, Length=152, Percent_Identity=33.5526315789474, Blast_Score=67, Evalue=5e-11,
Organism=Escherichia coli, GI1788595, Length=458, Percent_Identity=32.3144104803493, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI145693145, Length=509, Percent_Identity=24.3614931237721, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI1788107, Length=506, Percent_Identity=21.7391304347826, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1786801, Length=429, Percent_Identity=22.6107226107226, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1786810, Length=510, Percent_Identity=23.921568627451, Blast_Score=79, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17558820, Length=454, Percent_Identity=27.0925110132159, Blast_Score=145, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI32563687, Length=462, Percent_Identity=25.974025974026, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17560308, Length=330, Percent_Identity=26.3636363636364, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI71994690, Length=356, Percent_Identity=23.876404494382, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI71994703, Length=356, Percent_Identity=23.5955056179775, Blast_Score=96, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI71994694, Length=356, Percent_Identity=23.876404494382, Blast_Score=95, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17557194, Length=504, Percent_Identity=22.6190476190476, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17559526, Length=263, Percent_Identity=29.6577946768061, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17560140, Length=446, Percent_Identity=22.8699551569507, Blast_Score=70, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI71983001, Length=542, Percent_Identity=23.6162361623616, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI71982997, Length=542, Percent_Identity=23.8007380073801, Blast_Score=69, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6319699, Length=350, Percent_Identity=24.8571428571429, Blast_Score=80, Evalue=5e-16,
Organism=Drosophila melanogaster, GI19922652, Length=493, Percent_Identity=25.7606490872211, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24581924, Length=491, Percent_Identity=24.0325865580448, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21355181, Length=468, Percent_Identity=23.0769230769231, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24648260, Length=404, Percent_Identity=25.4950495049505, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI20130357, Length=442, Percent_Identity=22.8506787330317, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24653035, Length=290, Percent_Identity=25.5172413793103, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI21356947, Length=438, Percent_Identity=23.2876712328767, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI19921316, Length=217, Percent_Identity=26.7281105990783, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 49153; Mature: 49153

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELVTQAAQTFGDQPALITDERRWSFADLDGDTARIATAFEASSIRRGDIVALVAPNSPA
CCHHHHHHHHCCCCCCEEECCCCCEEECCCCCHHHEEEHHHHCCCCCCCEEEEECCCCCH
LVLSLMALMRMGAVAAPVNHRFPANHIEGVLARLNPAMTLDAAKLDAFVADAIARTGATF
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCEECHHHHHHHHHHHHHHCCCCE
TAATEMERPVSVIHTSASSGKPKAAVHSLSNHYHSAMGSAQNLPFGPGDCWLLSLPLYHV
EEHHHCCCCHHHEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECHHHHH
GGYSMLFKCLLGGGALAVPSPDAALAESLTHFPVTHLSLVPTQLYRMLRADGGPERLRSL
CCHHHHHHHHHCCCEEECCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHH
RALLLGGSAVSAPLLREAICERVPLYLTYGSTEMSTQVTTSPTPVTKARGDSGVVLPYRE
HHHHHCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCEEECCCCCCHHCCCCCCEEEEHHH
VAISVDGEILVKGECLFMGYLDNGELREARDKNGWFHTGDMGELSGDARLTVLGRKDNMF
EEEEECCEEEEECEEEEEEEECCCCHHHHHCCCCCEECCCCCCCCCCCEEEEEECCCCEE
ISGGENIHPEEIEKALTSIVGIEEAVVVPAPDAEYGMRPVAWIKARSDSPDDATIIASLK
EECCCCCCHHHHHHHHHHHHCCCCEEEECCCCHHHCCCCEEEEEECCCCCCCHHHHHHHH
STIGKLKTPVAFHRIQEWQTIPGSAKIDRSWYRKLAEK
HHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MELVTQAAQTFGDQPALITDERRWSFADLDGDTARIATAFEASSIRRGDIVALVAPNSPA
CCHHHHHHHHCCCCCCEEECCCCCEEECCCCCHHHEEEHHHHCCCCCCCEEEEECCCCCH
LVLSLMALMRMGAVAAPVNHRFPANHIEGVLARLNPAMTLDAAKLDAFVADAIARTGATF
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCEECHHHHHHHHHHHHHHCCCCE
TAATEMERPVSVIHTSASSGKPKAAVHSLSNHYHSAMGSAQNLPFGPGDCWLLSLPLYHV
EEHHHCCCCHHHEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECHHHHH
GGYSMLFKCLLGGGALAVPSPDAALAESLTHFPVTHLSLVPTQLYRMLRADGGPERLRSL
CCHHHHHHHHHCCCEEECCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHH
RALLLGGSAVSAPLLREAICERVPLYLTYGSTEMSTQVTTSPTPVTKARGDSGVVLPYRE
HHHHHCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCEEECCCCCCHHCCCCCCEEEEHHH
VAISVDGEILVKGECLFMGYLDNGELREARDKNGWFHTGDMGELSGDARLTVLGRKDNMF
EEEEECCEEEEECEEEEEEEECCCCHHHHHCCCCCEECCCCCCCCCCCEEEEEECCCCEE
ISGGENIHPEEIEKALTSIVGIEEAVVVPAPDAEYGMRPVAWIKARSDSPDDATIIASLK
EECCCCCCHHHHHHHHHHHHCCCCEEEECCCCHHHCCCCEEEEEECCCCCCCHHHHHHHH
STIGKLKTPVAFHRIQEWQTIPGSAKIDRSWYRKLAEK
HHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609; 8206837 [H]