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The map label for this gene is purU [H]

Identifier: 21674639

GI number: 21674639

Start: 1729339

End: 1730208

Strand: Reverse

Name: purU [H]

Synonym: CT1825

Alternate gene names: 21674639

Gene position: 1730208-1729339 (Counterclockwise)

Preceding gene: 21674640

Following gene: 21674638

Centisome position: 80.29

GC content: 60.57

Gene sequence:

>870_bases
ATGATCGCTGCCGCCGCTCCCTCTAAAGCCATACTTCTGCTCTCCTGCCCCGACCGCGTGGGTCTTGTGGCGCGAATTGC
CAACTTTATCTACGAGCGCGGTGGAAACATTCTCGACCTCAACGAGCATGTCGATGTGGATGAACGGCAATTCTTTCTCC
GCGTTTCGTGGAGTCTTGACCACTTCTCGATTCCTGCTGAAGACCTCGAATCGGCATTTGCGCCGCTGGGCCGAGAATTC
AGGGCGAACTGGCAGATTCGCCTCTCTGGCAAGCGGAGCCGGATGGCGGTTTTCGTCTCGAAATACGACCACTGCCTGCG
CGAGATTCTCTGGCGGCACAGCCTCGGCGAGTTCGATATCGACCTCCCGCTCGTCATCTCCAATCATCCCGATCTCGCGC
CGCTCGTCGAAGCGCACGGCATACCGTTCCACGTCATTCCGGTGACGCCTGAAGCCAAAGCAGCAGCTGAGCAGCGGCAG
ATGGCTTTGTGCGATGAGCATGGCATTGACACCATCGTGCTGGCGCGGTACATGCAGGTGCTCTCGCCGGAGTTTACACG
GCGCTGGGTGGGCCGGATCATTAACATTCACCACTCGTTTCTTCCGGCCTTTGTCGGCGGCAACCCGTACCGGCAAGCCT
ACCGGCGGGGCGTGAAGCTGATCGGCGCGACCAGCCACTACGTCACCGACGAGTTGGACGAAGGGCCGATCATCGAGCAA
GATATCATCCGCATCACGCACCGCGACACGCTTGAAGACCTCGTGCGGAAAGGGCGCGACCTCGAACGGCTCGTGCTGGC
GCGCGCCCTGCGGCTGCACTGCGACCACCGGATACTGCTCAACGGGCGCAAAACCGTCGTGTTCGACTGA

Upstream 100 bases:

>100_bases
TACGATAATCTGCGAAGAACCCCTTGGTGAAACCAATAGGCGGATAACTTCATTATCTTGCTGCATTTTAATCACTAATG
TCAAGTTCAATTTTTCATCC

Downstream 100 bases:

>100_bases
GCTGATCCGTCCGGCGGGAATTTCCCGGGCCGCTCGAACAGTTCCTGTAAGACGAAGTATTGTTTGTCAGATCGGAATCC
GAATGAGCGAGCACCATCAC

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MIAAAAPSKAILLLSCPDRVGLVARIANFIYERGGNILDLNEHVDVDERQFFLRVSWSLDHFSIPAEDLESAFAPLGREF
RANWQIRLSGKRSRMAVFVSKYDHCLREILWRHSLGEFDIDLPLVISNHPDLAPLVEAHGIPFHVIPVTPEAKAAAEQRQ
MALCDEHGIDTIVLARYMQVLSPEFTRRWVGRIINIHHSFLPAFVGGNPYRQAYRRGVKLIGATSHYVTDELDEGPIIEQ
DIIRITHRDTLEDLVRKGRDLERLVLARALRLHCDHRILLNGRKTVVFD

Sequences:

>Translated_289_residues
MIAAAAPSKAILLLSCPDRVGLVARIANFIYERGGNILDLNEHVDVDERQFFLRVSWSLDHFSIPAEDLESAFAPLGREF
RANWQIRLSGKRSRMAVFVSKYDHCLREILWRHSLGEFDIDLPLVISNHPDLAPLVEAHGIPFHVIPVTPEAKAAAEQRQ
MALCDEHGIDTIVLARYMQVLSPEFTRRWVGRIINIHHSFLPAFVGGNPYRQAYRRGVKLIGATSHYVTDELDEGPIIEQ
DIIRITHRDTLEDLVRKGRDLERLVLARALRLHCDHRILLNGRKTVVFD
>Mature_289_residues
MIAAAAPSKAILLLSCPDRVGLVARIANFIYERGGNILDLNEHVDVDERQFFLRVSWSLDHFSIPAEDLESAFAPLGREF
RANWQIRLSGKRSRMAVFVSKYDHCLREILWRHSLGEFDIDLPLVISNHPDLAPLVEAHGIPFHVIPVTPEAKAAAEQRQ
MALCDEHGIDTIVLARYMQVLSPEFTRRWVGRIINIHHSFLPAFVGGNPYRQAYRRGVKLIGATSHYVTDELDEGPIIEQ
DIIRITHRDTLEDLVRKGRDLERLVLARALRLHCDHRILLNGRKTVVFD

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=185, Percent_Identity=31.8918918918919, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI209869995, Length=185, Percent_Identity=31.8918918918919, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI209869993, Length=185, Percent_Identity=31.8918918918919, Blast_Score=94, Evalue=1e-19,
Organism=Escherichia coli, GI1787483, Length=282, Percent_Identity=42.5531914893617, Blast_Score=221, Evalue=3e-59,
Organism=Escherichia coli, GI1788846, Length=191, Percent_Identity=28.2722513089005, Blast_Score=82, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17567511, Length=169, Percent_Identity=32.5443786982249, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24582400, Length=208, Percent_Identity=33.1730769230769, Blast_Score=85, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF01842 ACT; PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 32984; Mature: 32984

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAAAAPSKAILLLSCPDRVGLVARIANFIYERGGNILDLNEHVDVDERQFFLRVSWSLD
CCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCEEEEEEEECCC
HFSIPAEDLESAFAPLGREFRANWQIRLSGKRSRMAVFVSKYDHCLREILWRHSLGEFDI
CCCCCHHHHHHHHHHCCHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
DLPLVISNHPDLAPLVEAHGIPFHVIPVTPEAKAAAEQRQMALCDEHGIDTIVLARYMQV
CCEEEECCCCCHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
LSPEFTRRWVGRIINIHHSFLPAFVGGNPYRQAYRRGVKLIGATSHYVTDELDEGPIIEQ
HCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCEEEECCCHHHCCCCCCCCCCHH
DIIRITHRDTLEDLVRKGRDLERLVLARALRLHCDHRILLNGRKTVVFD
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEECCCEEEEEC
>Mature Secondary Structure
MIAAAAPSKAILLLSCPDRVGLVARIANFIYERGGNILDLNEHVDVDERQFFLRVSWSLD
CCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCEEEEEEEECCC
HFSIPAEDLESAFAPLGREFRANWQIRLSGKRSRMAVFVSKYDHCLREILWRHSLGEFDI
CCCCCHHHHHHHHHHCCHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
DLPLVISNHPDLAPLVEAHGIPFHVIPVTPEAKAAAEQRQMALCDEHGIDTIVLARYMQV
CCEEEECCCCCHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
LSPEFTRRWVGRIINIHHSFLPAFVGGNPYRQAYRRGVKLIGATSHYVTDELDEGPIIEQ
HCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCEEEECCCHHHCCCCCCCCCCHH
DIIRITHRDTLEDLVRKGRDLERLVLARALRLHCDHRILLNGRKTVVFD
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEECCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]