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The map label for this gene is queF

Identifier: 21674456

GI number: 21674456

Start: 1547771

End: 1548124

Strand: Direct

Name: queF

Synonym: CT1638

Alternate gene names: 21674456

Gene position: 1547771-1548124 (Clockwise)

Preceding gene: 21674446

Following gene: 21674458

Centisome position: 71.82

GC content: 51.98

Gene sequence:

>354_bases
ATGAACAAAGAGATCATCGAAGTTTTCGACAACACCTATCCCGACAGGGACTACACTATCGAAATCATCAACCCCGAATT
CACCTCGGTCTGCCCCAAAACCGGCCTGCCCGACTTCGGCACAATCACCGTGAATTACGTGCCCGACAAAAGCTGCATCG
AGCTCAAGTCCCTCAAATACTACTTCCTCGAATTCCGCAACGCTGGCATCTTCTACGAGAACATCACCAACCGGATTCTC
GACGACCTTGTTGAAGCCTGCCAGCCGAGAAGAATGACCGTCAAAACCGAGTGGAACGCCAGAGGCGGCATCACCGAAAC
CGTCACCGTCAGCTACAGCAAGAGCAAAGAGTAA

Upstream 100 bases:

>100_bases
TCCTGAGGGTCGATCTGGTCACAAATTTAATGAATATCACTGCCGTTTTCTATTTTGCAGCGCTTATGAGCAATCACCTT
AACCTATCCGGCTTATACCC

Downstream 100 bases:

>100_bases
CCTGTCCAATGCAGAACGCCCCGGATTTCCGGGGCGCTTCTGAAAGAAAACCTCTGTTGTTCATTCCGATCGGAATCAGA
ACTTCTCGTGGAGCCAGATG

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase

Number of amino acids: Translated: 117; Mature: 117

Protein sequence:

>117_residues
MNKEIIEVFDNTYPDRDYTIEIINPEFTSVCPKTGLPDFGTITVNYVPDKSCIELKSLKYYFLEFRNAGIFYENITNRIL
DDLVEACQPRRMTVKTEWNARGGITETVTVSYSKSKE

Sequences:

>Translated_117_residues
MNKEIIEVFDNTYPDRDYTIEIINPEFTSVCPKTGLPDFGTITVNYVPDKSCIELKSLKYYFLEFRNAGIFYENITNRIL
DDLVEACQPRRMTVKTEWNARGGITETVTVSYSKSKE
>Mature_117_residues
MNKEIIEVFDNTYPDRDYTIEIINPEFTSVCPKTGLPDFGTITVNYVPDKSCIELKSLKYYFLEFRNAGIFYENITNRIL
DDLVEACQPRRMTVKTEWNARGGITETVTVSYSKSKE

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1)

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEF_CHLTE (Q9F719)

Other databases:

- EMBL:   AF287481
- EMBL:   AE006470
- RefSeq:   NP_662521.1
- ProteinModelPortal:   Q9F719
- SMR:   Q9F719
- GeneID:   1006126
- GenomeReviews:   AE006470_GR
- KEGG:   cte:CT1638
- NMPDR:   fig|194439.1.peg.1615
- TIGR:   CT1638
- HOGENOM:   HBG294103
- OMA:   FRNAGIF
- ProtClustDB:   PRK13258
- BioCyc:   CTEP194439:CT_1638-MONOMER
- BRENDA:   1.7.1.13
- GO:   GO:0005737
- HAMAP:   MF_00818
- InterPro:   IPR016856
- InterPro:   IPR020602
- PIRSF:   PIRSF027377
- TIGRFAMs:   TIGR03139

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =1.7.1.13

Molecular weight: Translated: 13565; Mature: 13565

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKEIIEVFDNTYPDRDYTIEIINPEFTSVCPKTGLPDFGTITVNYVPDKSCIELKSLKY
CCCHHHEECCCCCCCCCEEEEEECCCHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHHHE
YFLEFRNAGIFYENITNRILDDLVEACQPRRMTVKTEWNARGGITETVTVSYSKSKE
EEEEECCCCEEHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEEEEECCCCC
>Mature Secondary Structure
MNKEIIEVFDNTYPDRDYTIEIINPEFTSVCPKTGLPDFGTITVNYVPDKSCIELKSLKY
CCCHHHEECCCCCCCCCEEEEEECCCHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHHHE
YFLEFRNAGIFYENITNRILDDLVEACQPRRMTVKTEWNARGGITETVTVSYSKSKE
EEEEECCCCEEHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10976061; 12093901