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The map label for this gene is appA [H]

Identifier: 21674450

GI number: 21674450

Start: 1541788

End: 1543416

Strand: Reverse

Name: appA [H]

Synonym: CT1632

Alternate gene names: 21674450

Gene position: 1543416-1541788 (Counterclockwise)

Preceding gene: 21674451

Following gene: 21674449

Centisome position: 71.62

GC content: 57.34

Gene sequence:

>1629_bases
ATGCTTGGTGACGCCGATTACCTCAATCCGGTCATCGGGGCGTCGCTCACGTCGAGCGAGATCACCGGGCTGATCTATCC
GGCTCTTCTTCAGGGTGAGTTCGACACCAAAACCGGCCTGCTCAACTACCTTGCGCTTGAAAAACGGTTGCGCTCTTCCA
CCGGCCCCGACGAGAAGTCGCCGAAGGGCGCGCTCGCCAAAACCTGGACGATGTCGCCCGACCATCGCTCCATCACCTAC
ATTCTGCGTGATGATGCGAAGTGGGCTGATGGCCAGCCAATCACGTCGCGTGACTTCAAGTTTACCTACAAGCTCTATGG
CAATCCGGTGATCGCCAGCCCTCGCCAGCAGTTTCTTGCCGAACTGGTCGGCGCGGACAAGGGGCAGGTCGATTTCGACA
GGGCGATCGAAACGCCAAACGACACCACGCTCATCTTCCATTTCTACAAGCCGGTACCGGAGCATCTTGCGCTCTTCCAC
ACATCGCTGACACCACTGCCGGAGCATCTCTGGAAAAACGTCAAGCCGGAGGAGTTCCGCGAGTCGAAGCTCAACCAGCA
GCCGGTGGGCGCTGGCCCGTACCGCCTCGCCGACTGGAGCAAGCAGCAGTCGCTAACCCTTTCGTCGAACGTCTCCTGCA
ACCTGCCCAAGCCGGGCAACATCAAACGCATCATCTACCGCGTCATTCCCGACTACACCGTCCGGTTGGCACAGCTCCAG
ACGGGTGATGTCGATGTGGTCGAGAATATCAAACCGGAGGATTTCGCCGCCGTGCAGAAGGCCAATCCGAATGTCGATAT
AAAAACCATCGGTTTGCGGGTTTACGACTATGTTGGCTGGCAGAATATCGACGGCGCGTATTACAACCAGACCGGCAAGA
TTCGCCCCCATCCGCTCTTCGGTGATCCGGTCGTGCGCCGTGCGCTGACCATGGCTATTGACCGCCAGTCGATCATCGAC
GGCTATCTCGGCGAGTACGGCGTGCTGGCCAAAACCGATATTTCGCCTTCGCTGAAGTGGGCTTACGACGACTCGATCAA
GCCCTATGGCTACGATCCAGCGCAAGCCGTCAAGTTGCTCGAAGCAGCGGGATGGATGCCGGGCCCCGATGGCATCCGGC
AGAAGAACGGGCGCAAGTTTAGCTTCGTGCTCTACACGAACGCCGGAAACGCGCGCAGGAACTACGCCTGTACGATCATC
CAGCAAAACCTTCGTGAGATCGGCATCGACTGCAAGATCGAAATGCAGGAGTCCAACGTTTTCTTCCAGAACCTGCAGGA
TCGAAAGCTCGATGCCTGGATGGCGGGCTGGTCGATCGGCCTTGAGATCGATCCGCTCGACGTGTGGGGTTCCGACCTGA
AAAAGAGCCGTTTCAACTTTCCCGGTTTCATCAATCCGAGGATCGACCAACTCTGCGAACTTGCCAAGAACAAGATGCGC
ATTGAAGACGCGCGTCCTTACTGGATCGAGTACCAGAAGATTCTGCACGAACAGCAGCCGGTCACCTTCCTCTACTGGAT
CAGGGAGACGCAAGGGTTCAGCAAGCGTATTCAGGGCGCGAAGCTCAATATTTCCGGTACTTTCTACAACATCGACGACT
GGACGCTGAAACCGTCAATCGCGCCGTAA

Upstream 100 bases:

>100_bases
GCTGCTTGCGGCCTCAACCCTGTTGACGGCCTGTTCGAAGTCTGGCGGTAAATCCGGTGCACTTTCGGGCGCGGCTCGCG
ACACCACGCTGGTGATCGGA

Downstream 100 bases:

>100_bases
CGACATGCTCAGGTATATCTTCAAACGGCTCTTGATTGCCGTGCCGCTCATCTTCGGGGTGCTGACCCTGACCTTCTTCA
TCATCCGGCTCGCGCCGGGC

Product: peptide ABC transporter, periplasmic peptide-binding protein

Products: Ni2; [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 542; Mature: 542

Protein sequence:

>542_residues
MLGDADYLNPVIGASLTSSEITGLIYPALLQGEFDTKTGLLNYLALEKRLRSSTGPDEKSPKGALAKTWTMSPDHRSITY
ILRDDAKWADGQPITSRDFKFTYKLYGNPVIASPRQQFLAELVGADKGQVDFDRAIETPNDTTLIFHFYKPVPEHLALFH
TSLTPLPEHLWKNVKPEEFRESKLNQQPVGAGPYRLADWSKQQSLTLSSNVSCNLPKPGNIKRIIYRVIPDYTVRLAQLQ
TGDVDVVENIKPEDFAAVQKANPNVDIKTIGLRVYDYVGWQNIDGAYYNQTGKIRPHPLFGDPVVRRALTMAIDRQSIID
GYLGEYGVLAKTDISPSLKWAYDDSIKPYGYDPAQAVKLLEAAGWMPGPDGIRQKNGRKFSFVLYTNAGNARRNYACTII
QQNLREIGIDCKIEMQESNVFFQNLQDRKLDAWMAGWSIGLEIDPLDVWGSDLKKSRFNFPGFINPRIDQLCELAKNKMR
IEDARPYWIEYQKILHEQQPVTFLYWIRETQGFSKRIQGAKLNISGTFYNIDDWTLKPSIAP

Sequences:

>Translated_542_residues
MLGDADYLNPVIGASLTSSEITGLIYPALLQGEFDTKTGLLNYLALEKRLRSSTGPDEKSPKGALAKTWTMSPDHRSITY
ILRDDAKWADGQPITSRDFKFTYKLYGNPVIASPRQQFLAELVGADKGQVDFDRAIETPNDTTLIFHFYKPVPEHLALFH
TSLTPLPEHLWKNVKPEEFRESKLNQQPVGAGPYRLADWSKQQSLTLSSNVSCNLPKPGNIKRIIYRVIPDYTVRLAQLQ
TGDVDVVENIKPEDFAAVQKANPNVDIKTIGLRVYDYVGWQNIDGAYYNQTGKIRPHPLFGDPVVRRALTMAIDRQSIID
GYLGEYGVLAKTDISPSLKWAYDDSIKPYGYDPAQAVKLLEAAGWMPGPDGIRQKNGRKFSFVLYTNAGNARRNYACTII
QQNLREIGIDCKIEMQESNVFFQNLQDRKLDAWMAGWSIGLEIDPLDVWGSDLKKSRFNFPGFINPRIDQLCELAKNKMR
IEDARPYWIEYQKILHEQQPVTFLYWIRETQGFSKRIQGAKLNISGTFYNIDDWTLKPSIAP
>Mature_542_residues
MLGDADYLNPVIGASLTSSEITGLIYPALLQGEFDTKTGLLNYLALEKRLRSSTGPDEKSPKGALAKTWTMSPDHRSITY
ILRDDAKWADGQPITSRDFKFTYKLYGNPVIASPRQQFLAELVGADKGQVDFDRAIETPNDTTLIFHFYKPVPEHLALFH
TSLTPLPEHLWKNVKPEEFRESKLNQQPVGAGPYRLADWSKQQSLTLSSNVSCNLPKPGNIKRIIYRVIPDYTVRLAQLQ
TGDVDVVENIKPEDFAAVQKANPNVDIKTIGLRVYDYVGWQNIDGAYYNQTGKIRPHPLFGDPVVRRALTMAIDRQSIID
GYLGEYGVLAKTDISPSLKWAYDDSIKPYGYDPAQAVKLLEAAGWMPGPDGIRQKNGRKFSFVLYTNAGNARRNYACTII
QQNLREIGIDCKIEMQESNVFFQNLQDRKLDAWMAGWSIGLEIDPLDVWGSDLKKSRFNFPGFINPRIDQLCELAKNKMR
IEDARPYWIEYQKILHEQQPVTFLYWIRETQGFSKRIQGAKLNISGTFYNIDDWTLKPSIAP

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789887, Length=379, Percent_Identity=29.023746701847, Blast_Score=116, Evalue=4e-27,
Organism=Escherichia coli, GI1789966, Length=477, Percent_Identity=23.6897274633124, Blast_Score=105, Evalue=9e-24,
Organism=Escherichia coli, GI1787762, Length=498, Percent_Identity=24.6987951807229, Blast_Score=98, Evalue=2e-21,
Organism=Escherichia coli, GI1787495, Length=434, Percent_Identity=24.1935483870968, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI87081878, Length=454, Percent_Identity=23.1277533039648, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1787052, Length=475, Percent_Identity=23.1578947368421, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1789397, Length=320, Percent_Identity=25, Blast_Score=70, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 61555; Mature: 61555

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: PS01159 WW_DOMAIN_1 ; PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGDADYLNPVIGASLTSSEITGLIYPALLQGEFDTKTGLLNYLALEKRLRSSTGPDEKS
CCCCCHHCCHHHCCCCCHHHHHHHEEHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCC
PKGALAKTWTMSPDHRSITYILRDDAKWADGQPITSRDFKFTYKLYGNPVIASPRQQFLA
CCCCCEEEEECCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEEEEECCCEECCHHHHHHH
ELVGADKGQVDFDRAIETPNDTTLIFHFYKPVPEHLALFHTSLTPLPEHLWKNVKPEEFR
HHHCCCCCCCCHHHHCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCHHHHHCCCCHHHHH
ESKLNQQPVGAGPYRLADWSKQQSLTLSSNVSCNLPKPGNIKRIIYRVIPDYTVRLAQLQ
HHHCCCCCCCCCCCCCCCCCCCCCEEEECCCEECCCCCCCHHHHHHHHCCCCEEEEEEEC
TGDVDVVENIKPEDFAAVQKANPNVDIKTIGLRVYDYVGWQNIDGAYYNQTGKIRPHPLF
CCCCHHHHCCCCCHHHHHHCCCCCCEEEEEEEEEEEECCCCCCCCCEECCCCCCCCCCCC
GDPVVRRALTMAIDRQSIIDGYLGEYGVLAKTDISPSLKWAYDDSIKPYGYDPAQAVKLL
CCHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCEEEEECCCCCCCCCCHHHHHHHH
EAAGWMPGPDGIRQKNGRKFSFVLYTNAGNARRNYACTIIQQNLREIGIDCKIEMQESNV
HHHCCCCCCCCHHHCCCCEEEEEEEECCCCCCCCCEEHHHHHHHHHCCCCEEEEEECCCC
FFQNLQDRKLDAWMAGWSIGLEIDPLDVWGSDLKKSRFNFPGFINPRIDQLCELAKNKMR
HHHCCCCCHHHHHHCCEEECEEECCHHHCCCHHHHHCCCCCCCCCHHHHHHHHHHHHCEE
IEDARPYWIEYQKILHEQQPVTFLYWIRETQGFSKRIQGAKLNISGTFYNIDDWTLKPSI
ECCCCCHHHHHHHHHHCCCCCEEEEEEECCCCHHHHCCCCEEEEEEEEEECCCCEECCCC
AP
CC
>Mature Secondary Structure
MLGDADYLNPVIGASLTSSEITGLIYPALLQGEFDTKTGLLNYLALEKRLRSSTGPDEKS
CCCCCHHCCHHHCCCCCHHHHHHHEEHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCC
PKGALAKTWTMSPDHRSITYILRDDAKWADGQPITSRDFKFTYKLYGNPVIASPRQQFLA
CCCCCEEEEECCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEEEEECCCEECCHHHHHHH
ELVGADKGQVDFDRAIETPNDTTLIFHFYKPVPEHLALFHTSLTPLPEHLWKNVKPEEFR
HHHCCCCCCCCHHHHCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCHHHHHCCCCHHHHH
ESKLNQQPVGAGPYRLADWSKQQSLTLSSNVSCNLPKPGNIKRIIYRVIPDYTVRLAQLQ
HHHCCCCCCCCCCCCCCCCCCCCCEEEECCCEECCCCCCCHHHHHHHHCCCCEEEEEEEC
TGDVDVVENIKPEDFAAVQKANPNVDIKTIGLRVYDYVGWQNIDGAYYNQTGKIRPHPLF
CCCCHHHHCCCCCHHHHHHCCCCCCEEEEEEEEEEEECCCCCCCCCEECCCCCCCCCCCC
GDPVVRRALTMAIDRQSIIDGYLGEYGVLAKTDISPSLKWAYDDSIKPYGYDPAQAVKLL
CCHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCEEEEECCCCCCCCCCHHHHHHHH
EAAGWMPGPDGIRQKNGRKFSFVLYTNAGNARRNYACTIIQQNLREIGIDCKIEMQESNV
HHHCCCCCCCCHHHCCCCEEEEEEEECCCCCCCCCEEHHHHHHHHHCCCCEEEEEECCCC
FFQNLQDRKLDAWMAGWSIGLEIDPLDVWGSDLKKSRFNFPGFINPRIDQLCELAKNKMR
HHHCCCCCHHHHHHCCEEECEEECCHHHCCCHHHHHCCCCCCCCCHHHHHHHHHHHHCEE
IEDARPYWIEYQKILHEQQPVTFLYWIRETQGFSKRIQGAKLNISGTFYNIDDWTLKPSI
ECCCCCHHHHHHHHHHCCCCCEEEEEEECCCCHHHHCCCCEEEEEEEEEECCCCEECCCC
AP
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; ATP; H2O [C]

Specific reaction: Ni2+ [Periplasm] + ATP + H2O = Ni2+ [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]