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The map label for this gene is relA [H]

Identifier: 21674364

GI number: 21674364

Start: 1449578

End: 1451773

Strand: Reverse

Name: relA [H]

Synonym: CT1545

Alternate gene names: 21674364

Gene position: 1451773-1449578 (Counterclockwise)

Preceding gene: 21674365

Following gene: 21674351

Centisome position: 67.37

GC content: 55.51

Gene sequence:

>2196_bases
ATGCTGGCCCAGATAGAACAGGAACATTATTCCAAACTGCACGAAATATTGCGGCTTTGCCGGGCGAACCTCAAGAACTA
CGACGAGTCGCTCATCCAGCGTGCATTTTTCATGTGCTACCGGGCTCATGAGGGTGAAAAACGCGCATCCGGGGAGCCGT
TTTTCTACCATCCGGTCGAGGTCGCCAAGCTGCTGGTGACCGAACTGCCGCTTGACGGCGTCTCTGTTGCGGCGGCGCTG
CTGCATGACGTCATCGAGGATAGCGGTTACACCTATGAGGATATTTCCGCCGAGCTGGGTGCCGAAGTGGCTGACATCGT
GGAGGGGCTGACCAAGATTTCCGAAATCATGGTCAACCGCGAAACCACCGAGGCCGAAGGGTTCCGCAAGATGCTGCTCT
CGATGGTCAAGGATATTCGCGTCATCCTGATCAAATTCTGCGACCGCCTGCACAACATGCGCACCCTCGACTCCCTGCCG
GAGCATCGCCGCCTGCGCATGGCGCTCGAAACGCGCGATATCTATGCGCCGCTCGCTCACCGTTTCGGTCTCGGTAAAAT
GAAGGTCGATCTGGAGAACCTTGCTCTCAAGTACATCGATCCCGAGATGTACGACTACCTGCTCAAGAAAGTGCGCCTGA
GCCGCAACGAGCGCGTCGCCTATCTCAACAAGATGATCGCTCCGATCAAGGATGATCTTGAAAAGCAGGGGTTTACCGTC
GAATTGCAGGGGCGCGCCAAGCATCTCTATTCGATTTACAACAAGATGCGGATGAAGAACAAGAAGTTTGACGACATCCA
CGATCTGTACGGCATTCGCGTCATCATCGATACGGAGAAGATTTCGGACTGCTTCGCTGTGTACGGCTACATCACGCAGC
AGTTTCCGCCCATTCCGCAGCACTTCAAGGATTACATCTCGATTCCGAAGCATAATGGCTACCAGTCGCTCCACTCGGCG
ATTATCGGTCCAAAAGGGCATGTCGTCGAGTTGCAGATCAGAACACGCCGGATGCACGAATTTGCCGAACTCGGTGTCGC
GGCTCACTGGCGCTACAAGGAGAAGATTTCCAAGGACGATGCGGCGCTCGATTCATTTCTCAAGTGGGCGAGGGAGCTGA
TCAAAGATGCCGATTCGGCTACGGCCTTCATGGAGGGGTTTAAACTCAACCTTTACCATGACGAGATTTACGTCTTCACC
CCGAAGGGCGATATGAAAGTGCTGCCCGCAGGAGCCACGCCGATCGATTTCGCCTATGCCATCCACTCCGAAATTGGCAA
TGGCTGCATTGGCGCGAAGGTCAATGGCAAAATCGTGCGGCTGAATACCGAGCTGAGGTCGGGCGATCGCGTCGAGGTGA
TCACCTCGAAGAGTCAGAGGCCGAAGGCGGACTGGCTGAAGATCGTGGTGACGCACCGCGCCAAACTCAAGATCAGGGCG
GCCATCAACGAGGAGCGCCGCCAGGAGATTGAAAAGGGGCGCAGCATCTGGGAAAAGATGCTCTCTGGCAGCAAGAAGCT
TTTCACCGAAAATGACGCCATTCGTGCCATCCGTCAGCATGGCATCAAGACCCCTGCCGATCTGTTCAACGCACTCGCCA
AACAGCAGATCAGCAGCGAAGAGGTGCTGGAGCGGATCAGCCACCCGCACAGACCCGCCGAGACTCACGAGAGCACCGTA
CAGGCCAGGGCGCCCCACAAGGATTTCGCCGAGATCGCTCGCGAGGTGCAGGAGCGTCTTGGGTACCAGAAGGATGAGGT
AACCATCGCTGGACTGAACAACATTTCCTACTCCTACGCCAAATGCTGCAATCCGGTGCCGGGCGACGACATCATCGGCT
TTGTGACCACCGGAGGCACGGTGAAGATTCACCGCAAGAACTGTGTCAACGTCACCAACGAGAACTCCGTCAAAAGCGAG
CGCATCGTCTCGGTTGCGTGGAACCGCAAGGTTGACACCGATTTTCTGGCAGGCATTCGCATCGTCGGCGAAGACAAGAT
CGGCATGACCAACCAGATCACCGGCGTCATCTCGAAATTTGACACGAACATCCGAACCATTGTCCTGAACGCCAAAGATG
GCATTTTCACCTGCAATCTGATGATCTTTGTCAAAAACACCGACAAACTGACGACGTTGATGGACAAGCTGCGGAAGGTG
CAGGGGGTGTTTACCGTGGAGCGGTTGTCGAACTGA

Upstream 100 bases:

>100_bases
CAGAGTAAAAAGTATTGACCTGAAGCCTCGATTTCATATCATAATAATAGTATAGAAAATCAGGCGATAACAACTCTTTA
TTACGGAGAGGCTTACCTCC

Downstream 100 bases:

>100_bases
TAGTGATGAAAATAATGTGAGATAGATATGGGCAGCGCTTTGTGGCTGCCCTTGTCAATTGATCAGAAAACAAGATCTTT
GCGAAGGGACTCCTCTATAT

Product: GTP pyrophosphokinase

Products: NA

Alternate protein names: (p)ppGpp synthase; ATP:GTP 3'-pyrophosphotransferase; ppGpp synthase I [H]

Number of amino acids: Translated: 731; Mature: 731

Protein sequence:

>731_residues
MLAQIEQEHYSKLHEILRLCRANLKNYDESLIQRAFFMCYRAHEGEKRASGEPFFYHPVEVAKLLVTELPLDGVSVAAAL
LHDVIEDSGYTYEDISAELGAEVADIVEGLTKISEIMVNRETTEAEGFRKMLLSMVKDIRVILIKFCDRLHNMRTLDSLP
EHRRLRMALETRDIYAPLAHRFGLGKMKVDLENLALKYIDPEMYDYLLKKVRLSRNERVAYLNKMIAPIKDDLEKQGFTV
ELQGRAKHLYSIYNKMRMKNKKFDDIHDLYGIRVIIDTEKISDCFAVYGYITQQFPPIPQHFKDYISIPKHNGYQSLHSA
IIGPKGHVVELQIRTRRMHEFAELGVAAHWRYKEKISKDDAALDSFLKWARELIKDADSATAFMEGFKLNLYHDEIYVFT
PKGDMKVLPAGATPIDFAYAIHSEIGNGCIGAKVNGKIVRLNTELRSGDRVEVITSKSQRPKADWLKIVVTHRAKLKIRA
AINEERRQEIEKGRSIWEKMLSGSKKLFTENDAIRAIRQHGIKTPADLFNALAKQQISSEEVLERISHPHRPAETHESTV
QARAPHKDFAEIAREVQERLGYQKDEVTIAGLNNISYSYAKCCNPVPGDDIIGFVTTGGTVKIHRKNCVNVTNENSVKSE
RIVSVAWNRKVDTDFLAGIRIVGEDKIGMTNQITGVISKFDTNIRTIVLNAKDGIFTCNLMIFVKNTDKLTTLMDKLRKV
QGVFTVERLSN

Sequences:

>Translated_731_residues
MLAQIEQEHYSKLHEILRLCRANLKNYDESLIQRAFFMCYRAHEGEKRASGEPFFYHPVEVAKLLVTELPLDGVSVAAAL
LHDVIEDSGYTYEDISAELGAEVADIVEGLTKISEIMVNRETTEAEGFRKMLLSMVKDIRVILIKFCDRLHNMRTLDSLP
EHRRLRMALETRDIYAPLAHRFGLGKMKVDLENLALKYIDPEMYDYLLKKVRLSRNERVAYLNKMIAPIKDDLEKQGFTV
ELQGRAKHLYSIYNKMRMKNKKFDDIHDLYGIRVIIDTEKISDCFAVYGYITQQFPPIPQHFKDYISIPKHNGYQSLHSA
IIGPKGHVVELQIRTRRMHEFAELGVAAHWRYKEKISKDDAALDSFLKWARELIKDADSATAFMEGFKLNLYHDEIYVFT
PKGDMKVLPAGATPIDFAYAIHSEIGNGCIGAKVNGKIVRLNTELRSGDRVEVITSKSQRPKADWLKIVVTHRAKLKIRA
AINEERRQEIEKGRSIWEKMLSGSKKLFTENDAIRAIRQHGIKTPADLFNALAKQQISSEEVLERISHPHRPAETHESTV
QARAPHKDFAEIAREVQERLGYQKDEVTIAGLNNISYSYAKCCNPVPGDDIIGFVTTGGTVKIHRKNCVNVTNENSVKSE
RIVSVAWNRKVDTDFLAGIRIVGEDKIGMTNQITGVISKFDTNIRTIVLNAKDGIFTCNLMIFVKNTDKLTTLMDKLRKV
QGVFTVERLSN
>Mature_731_residues
MLAQIEQEHYSKLHEILRLCRANLKNYDESLIQRAFFMCYRAHEGEKRASGEPFFYHPVEVAKLLVTELPLDGVSVAAAL
LHDVIEDSGYTYEDISAELGAEVADIVEGLTKISEIMVNRETTEAEGFRKMLLSMVKDIRVILIKFCDRLHNMRTLDSLP
EHRRLRMALETRDIYAPLAHRFGLGKMKVDLENLALKYIDPEMYDYLLKKVRLSRNERVAYLNKMIAPIKDDLEKQGFTV
ELQGRAKHLYSIYNKMRMKNKKFDDIHDLYGIRVIIDTEKISDCFAVYGYITQQFPPIPQHFKDYISIPKHNGYQSLHSA
IIGPKGHVVELQIRTRRMHEFAELGVAAHWRYKEKISKDDAALDSFLKWARELIKDADSATAFMEGFKLNLYHDEIYVFT
PKGDMKVLPAGATPIDFAYAIHSEIGNGCIGAKVNGKIVRLNTELRSGDRVEVITSKSQRPKADWLKIVVTHRAKLKIRA
AINEERRQEIEKGRSIWEKMLSGSKKLFTENDAIRAIRQHGIKTPADLFNALAKQQISSEEVLERISHPHRPAETHESTV
QARAPHKDFAEIAREVQERLGYQKDEVTIAGLNNISYSYAKCCNPVPGDDIIGFVTTGGTVKIHRKNCVNVTNENSVKSE
RIVSVAWNRKVDTDFLAGIRIVGEDKIGMTNQITGVISKFDTNIRTIVLNAKDGIFTCNLMIFVKNTDKLTTLMDKLRKV
QGVFTVERLSN

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the formation of pppGpp which i

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Escherichia coli, GI1790082, Length=708, Percent_Identity=37.5706214689266, Blast_Score=479, Evalue=1e-136,
Organism=Escherichia coli, GI1789147, Length=717, Percent_Identity=32.0781032078103, Blast_Score=399, Evalue=1e-112,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =2.7.6.5 [H]

Molecular weight: Translated: 83467; Mature: 83467

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAQIEQEHYSKLHEILRLCRANLKNYDESLIQRAFFMCYRAHEGEKRASGEPFFYHPVE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECHHH
VAKLLVTELPLDGVSVAAALLHDVIEDSGYTYEDISAELGAEVADIVEGLTKISEIMVNR
HHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCC
ETTEAEGFRKMLLSMVKDIRVILIKFCDRLHNMRTLDSLPEHRRLRMALETRDIYAPLAH
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCHHHHHHHHHHHHHHHHHHHH
RFGLGKMKVDLENLALKYIDPEMYDYLLKKVRLSRNERVAYLNKMIAPIKDDLEKQGFTV
HCCCCCEEEEHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEE
ELQGRAKHLYSIYNKMRMKNKKFDDIHDLYGIRVIIDTEKISDCFAVYGYITQQFPPIPQ
EECCHHHHHHHHHHHHHHCCCCCCHHHHHCCEEEEEECHHHHHHHHHHHHHHHCCCCCHH
HFKDYISIPKHNGYQSLHSAIIGPKGHVVELQIRTRRMHEFAELGVAAHWRYKEKISKDD
HHHHHHCCCCCCCHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHCCHHHHHHHHHHCCCCH
AALDSFLKWARELIKDADSATAFMEGFKLNLYHDEIYVFTPKGDMKVLPAGATPIDFAYA
HHHHHHHHHHHHHHHCCCHHHHHHCCCEEEEEECEEEEECCCCCEEEEECCCCCHHHHHH
IHSEIGNGCIGAKVNGKIVRLNTELRSGDRVEVITSKSQRPKADWLKIVVTHRAKLKIRA
HHHHHCCCEECCEECCEEEEEECCCCCCCEEEEEECCCCCCCHHHHHHHEECCCEEEEEH
AINEERRQEIEKGRSIWEKMLSGSKKLFTENDAIRAIRQHGIKTPADLFNALAKQQISSE
HHCHHHHHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHCCCCCHHHHHHHHHHHHCCHH
EVLERISHPHRPAETHESTVQARAPHKDFAEIAREVQERLGYQKDEVTIAGLNNISYSYA
HHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHH
KCCNPVPGDDIIGFVTTGGTVKIHRKNCVNVTNENSVKSERIVSVAWNRKVDTDFLAGIR
HHCCCCCCCCEEEEEECCCEEEEECCCCCCCCCCCCCCHHHEEEEEECCCCCHHHHHCEE
IVGEDKIGMTNQITGVISKFDTNIRTIVLNAKDGIFTCNLMIFVKNTDKLTTLMDKLRKV
EEECCCCCCHHHHHHHHHHHCCCEEEEEEECCCCEEEEEEEEEEECCHHHHHHHHHHHHH
QGVFTVERLSN
HHHHHHHHHCC
>Mature Secondary Structure
MLAQIEQEHYSKLHEILRLCRANLKNYDESLIQRAFFMCYRAHEGEKRASGEPFFYHPVE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECHHH
VAKLLVTELPLDGVSVAAALLHDVIEDSGYTYEDISAELGAEVADIVEGLTKISEIMVNR
HHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCC
ETTEAEGFRKMLLSMVKDIRVILIKFCDRLHNMRTLDSLPEHRRLRMALETRDIYAPLAH
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCHHHHHHHHHHHHHHHHHHHH
RFGLGKMKVDLENLALKYIDPEMYDYLLKKVRLSRNERVAYLNKMIAPIKDDLEKQGFTV
HCCCCCEEEEHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEE
ELQGRAKHLYSIYNKMRMKNKKFDDIHDLYGIRVIIDTEKISDCFAVYGYITQQFPPIPQ
EECCHHHHHHHHHHHHHHCCCCCCHHHHHCCEEEEEECHHHHHHHHHHHHHHHCCCCCHH
HFKDYISIPKHNGYQSLHSAIIGPKGHVVELQIRTRRMHEFAELGVAAHWRYKEKISKDD
HHHHHHCCCCCCCHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHCCHHHHHHHHHHCCCCH
AALDSFLKWARELIKDADSATAFMEGFKLNLYHDEIYVFTPKGDMKVLPAGATPIDFAYA
HHHHHHHHHHHHHHHCCCHHHHHHCCCEEEEEECEEEEECCCCCEEEEECCCCCHHHHHH
IHSEIGNGCIGAKVNGKIVRLNTELRSGDRVEVITSKSQRPKADWLKIVVTHRAKLKIRA
HHHHHCCCEECCEECCEEEEEECCCCCCCEEEEEECCCCCCCHHHHHHHEECCCEEEEEH
AINEERRQEIEKGRSIWEKMLSGSKKLFTENDAIRAIRQHGIKTPADLFNALAKQQISSE
HHCHHHHHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHCCCCCHHHHHHHHHHHHCCHH
EVLERISHPHRPAETHESTVQARAPHKDFAEIAREVQERLGYQKDEVTIAGLNNISYSYA
HHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHH
KCCNPVPGDDIIGFVTTGGTVKIHRKNCVNVTNENSVKSERIVSVAWNRKVDTDFLAGIR
HHCCCCCCCCEEEEEECCCEEEEECCCCCCCCCCCCCCHHHEEEEEECCCCCHHHHHCEE
IVGEDKIGMTNQITGVISKFDTNIRTIVLNAKDGIFTCNLMIFVKNTDKLTTLMDKLRKV
EEECCCCCCHHHHHHHHHHHCCCEEEEEEECCCCEEEEEEEEEEECCHHHHHHHHHHHHH
QGVFTVERLSN
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9383190; 9384377 [H]