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The map label for this gene is hisF [H]

Identifier: 21674334

GI number: 21674334

Start: 1420326

End: 1421081

Strand: Direct

Name: hisF [H]

Synonym: CT1514

Alternate gene names: 21674334

Gene position: 1420326-1421081 (Clockwise)

Preceding gene: 21674327

Following gene: 21674339

Centisome position: 65.91

GC content: 59.39

Gene sequence:

>756_bases
ATGTTAGCCAAACGCATCATACCCTGCCTTGACGTCCGCGACGGACGGGTGGTTAAAGGCATCAACTTCGAAGGCCTCAG
GGATGCCGGTTCGATTCTCGAACAGGCCCGCTTTTATAACAATGAAATGGCCGACGAGCTGGTCTTTCTCGACATCTCCG
CGTCGCTGGAATCAAGAAGGACAACCCTCGAAGAGGTACTGAAAGTTTCAGGCGAGGTGTTCATTCCGCTCACGGTCGGC
GGCGGTATCAGCTCGGTCGAGCGGGCGCACGACGCGTTCCTGCACGGCGCGGACAAGGTGTCGGTCAACACCGCCGCCGT
CAGCGAGCCGGAGTTGATCTCGCGCATCGCCGAAAAGTTCGGCTCGCAGGCGGTGGTGGTGGCGATTGATGTGAAAAAGG
TCGATGGGCGGTACATCGTTCATACTCATTCGGGCAAAAAGCCGACGGAGTACGAAGCGGTTGAATGGGCACACAAGGTG
CAGGAACTCGGCGCGGGCGAGATTCTCCTGACCAGCATGGATCGCGACGGCACGCAGGAGGGCTACGACAACGAAATTCT
CAAAATGATCTCGACCACCGTACACATTCCGGTGATCGCCTCCGGCGGCGCAGGCAACCTCGAACATCTCTACCGGGGAT
TTACGGATGGCCACGCCGACGCTGCGCTCGCGGCCTCGATCTTCCACTTCCGCACCTACTCGATCCGGCAGGCCAAGGAG
TACCTGCGCGAACGGGGAATCACGGTCAGGCTCTGA

Upstream 100 bases:

>100_bases
TGAATGCGGCTCAATATAAGAAGACTTTGATATATTGCGGCAGATTCAAAGTCATCCGATGTACCGGGCCGCTGTCCGGC
CAACCAACAGTATCGCGCAC

Downstream 100 bases:

>100_bases
ACCTCCGCATACGTAGCGATCGGCCCGGTCGCGACTCCCGGCTTATGAGACAGCTTCTCTTGCGTGGCGCAACATGCGGG
AAAACGCTATTTCGGCATGA

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MLAKRIIPCLDVRDGRVVKGINFEGLRDAGSILEQARFYNNEMADELVFLDISASLESRRTTLEEVLKVSGEVFIPLTVG
GGISSVERAHDAFLHGADKVSVNTAAVSEPELISRIAEKFGSQAVVVAIDVKKVDGRYIVHTHSGKKPTEYEAVEWAHKV
QELGAGEILLTSMDRDGTQEGYDNEILKMISTTVHIPVIASGGAGNLEHLYRGFTDGHADAALAASIFHFRTYSIRQAKE
YLRERGITVRL

Sequences:

>Translated_251_residues
MLAKRIIPCLDVRDGRVVKGINFEGLRDAGSILEQARFYNNEMADELVFLDISASLESRRTTLEEVLKVSGEVFIPLTVG
GGISSVERAHDAFLHGADKVSVNTAAVSEPELISRIAEKFGSQAVVVAIDVKKVDGRYIVHTHSGKKPTEYEAVEWAHKV
QELGAGEILLTSMDRDGTQEGYDNEILKMISTTVHIPVIASGGAGNLEHLYRGFTDGHADAALAASIFHFRTYSIRQAKE
YLRERGITVRL
>Mature_251_residues
MLAKRIIPCLDVRDGRVVKGINFEGLRDAGSILEQARFYNNEMADELVFLDISASLESRRTTLEEVLKVSGEVFIPLTVG
GGISSVERAHDAFLHGADKVSVNTAAVSEPELISRIAEKFGSQAVVVAIDVKKVDGRYIVHTHSGKKPTEYEAVEWAHKV
QELGAGEILLTSMDRDGTQEGYDNEILKMISTTVHIPVIASGGAGNLEHLYRGFTDGHADAALAASIFHFRTYSIRQAKE
YLRERGITVRL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=257, Percent_Identity=45.5252918287938, Blast_Score=228, Evalue=3e-61,
Organism=Escherichia coli, GI87082028, Length=211, Percent_Identity=25.1184834123223, Blast_Score=61, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6319725, Length=314, Percent_Identity=35.3503184713376, Blast_Score=152, Evalue=6e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 27552; Mature: 27552

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKRIIPCLDVRDGRVVKGINFEGLRDAGSILEQARFYNNEMADELVFLDISASLESRR
CCHHHCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHCCHHHCEEEEEEECCCHHHHH
TTLEEVLKVSGEVFIPLTVGGGISSVERAHDAFLHGADKVSVNTAAVSEPELISRIAEKF
HHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHCCCCCEEEEHHCCCCHHHHHHHHHHH
GSQAVVVAIDVKKVDGRYIVHTHSGKKPTEYEAVEWAHKVQELGAGEILLTSMDRDGTQE
CCCEEEEEEEEEECCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCC
GYDNEILKMISTTVHIPVIASGGAGNLEHLYRGFTDGHADAALAASIFHFRTYSIRQAKE
CCHHHHHHHHHHHEEEEEEECCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
YLRERGITVRL
HHHHCCCEEEC
>Mature Secondary Structure
MLAKRIIPCLDVRDGRVVKGINFEGLRDAGSILEQARFYNNEMADELVFLDISASLESRR
CCHHHCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHCCHHHCEEEEEEECCCHHHHH
TTLEEVLKVSGEVFIPLTVGGGISSVERAHDAFLHGADKVSVNTAAVSEPELISRIAEKF
HHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHCCCCCEEEEHHCCCCHHHHHHHHHHH
GSQAVVVAIDVKKVDGRYIVHTHSGKKPTEYEAVEWAHKVQELGAGEILLTSMDRDGTQE
CCCEEEEEEEEEECCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCC
GYDNEILKMISTTVHIPVIASGGAGNLEHLYRGFTDGHADAALAASIFHFRTYSIRQAKE
CCHHHHHHHHHHHEEEEEEECCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
YLRERGITVRL
HHHHCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA