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The map label for this gene is mpl [H]

Identifier: 21674299

GI number: 21674299

Start: 1385285

End: 1386709

Strand: Direct

Name: mpl [H]

Synonym: CT1479

Alternate gene names: 21674299

Gene position: 1385285-1386709 (Clockwise)

Preceding gene: 21674298

Following gene: 21674300

Centisome position: 64.28

GC content: 60.0

Gene sequence:

>1425_bases
ATGAGTTCCATTTACTTCATCGGCATCGGCGGCTCGGCAATGGCTTCGGTCGCCGTGGCGCTTTCGCACATGGGCCACGC
TGTCACCGGCTCGGACACGCAGCTCTATCCCCCGATGAGCACCTACCTCGAAAACCATAACATCCGCTATTTCAACAGCT
TTTCAGCAGAGAACCTGAAGAGCGCCACGCCCGACCTGGTGGTAGTAGGCAACGCGATAAGCCGGGGCAATCCTGACCTC
GAATATGCGCTCGATCAGCACATGGAGCTGATTTCGATGCCGCAACTGGTGCGGCGCGAGCTGATTGGTCGCCACACCTC
GATCGTGGTGGCGGGTACGCACGGCAAGACAACAACCACCTCGCTCGTGGCGTGGCTGCTCGAAGCGGGAGGGCTCCTGC
CGGGCTTCCTCATCGGCGGCATTCCCGAGAATTTCGGCGACGGCTGCCGTCCATCGGGCCTCTCAGAGCCGGGGTTTTTC
GTGACCGAGGGCGACGAGTACGACTCGGCCTTTTTCGACAAGCGCAGCAAGTTCCTGCACTACCGCCCCGACATCGCGAT
CATCAACAACGTCGAGTTCGACCATGCGGACATCTTCGATTCGCTCGACGACATCAAGAAAAGCTTCCGCCTGCTGGTGA
ACCTCGTGCCGTCGAATGGCTTGCTGATCGTCAACGCGGACGATCCGAATTCGATGGAGGTTTCCGCCAAGGCATTCTGC
CGCGTCGAGACCTTCGGCCTGAACGGTAACGCGGAGTGGACAGCGGCGGACATAGCGACAGATACCGATGGTACTTCGTT
CACCGTCGTGCGCGACAGCGAAGCGATCGGGCGGGTCAAAGTGCCGCTCTTCGGCAACTACAACGTGATGAATGCGCTCG
CCGCCACAGCGGCGGCAATCCGCGCTGGTGTGAGCTTCGAGTCGGTCACGCGCGGTCTTTGCAGCTTTAAGCGCCCGAAG
CGCCGCATGGAGCTCGTCGGCGAGTATGCGGGCGGCATCACGCTCATCGAGGATTTCGCGCACCATCCGACGGCCATCCG
CCTCACGCTCGGCGCAATTGCAGAGCATTACCCCGGTCGGCGTATCATCGCCTGTTTCGAGCCGCGCTCCAACACCACCA
GCCGCAACATTTTCCAGCACGAGCTGTCGGAATGCTTCGGCGATGCCGCGATCGTGGTGCTCGGCAAAGTAAACCGTCCG
GAGCGCTACGCCCCCGAAGAAAGGCTTGATGCAGCGCTGCTGTGCCGAGAGCTGAAGGCAAATGGCAAACGCGTTTTTGC
CGCAGGAAGCGAGAATTATCCGGAGGATATTGTCCGGTTCATCGAAGCTGAACAACAGCCGGGCGACGTGGTCGTGCTCC
TGAGCAACGGCAGTTTCAGCGGCCTGAAGGAGATGCTGGCCGAAAGTTTTCAAAAAAATTCATAA

Upstream 100 bases:

>100_bases
TCGATTTTACTTACTATTATTCATCGAAATCGATACCGGTTTTGAGAAAGAAAAAAGCCGGTGAGAGTTCACAGATGTTC
AACCAATCGCAAAACCATCC

Downstream 100 bases:

>100_bases
AGATTTCACCGGCGAAGTTCTATATCTTTTTATCTTGTGATTTTTGTTCAGTCCCGGAGAACGAGTGACGCCGTCGAGGC
AACAATCCGGAACCGGGCCG

Product: UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase

Products: NA

Alternate protein names: Murein peptide ligase [H]

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MSSIYFIGIGGSAMASVAVALSHMGHAVTGSDTQLYPPMSTYLENHNIRYFNSFSAENLKSATPDLVVVGNAISRGNPDL
EYALDQHMELISMPQLVRRELIGRHTSIVVAGTHGKTTTTSLVAWLLEAGGLLPGFLIGGIPENFGDGCRPSGLSEPGFF
VTEGDEYDSAFFDKRSKFLHYRPDIAIINNVEFDHADIFDSLDDIKKSFRLLVNLVPSNGLLIVNADDPNSMEVSAKAFC
RVETFGLNGNAEWTAADIATDTDGTSFTVVRDSEAIGRVKVPLFGNYNVMNALAATAAAIRAGVSFESVTRGLCSFKRPK
RRMELVGEYAGGITLIEDFAHHPTAIRLTLGAIAEHYPGRRIIACFEPRSNTTSRNIFQHELSECFGDAAIVVLGKVNRP
ERYAPEERLDAALLCRELKANGKRVFAAGSENYPEDIVRFIEAEQQPGDVVVLLSNGSFSGLKEMLAESFQKNS

Sequences:

>Translated_474_residues
MSSIYFIGIGGSAMASVAVALSHMGHAVTGSDTQLYPPMSTYLENHNIRYFNSFSAENLKSATPDLVVVGNAISRGNPDL
EYALDQHMELISMPQLVRRELIGRHTSIVVAGTHGKTTTTSLVAWLLEAGGLLPGFLIGGIPENFGDGCRPSGLSEPGFF
VTEGDEYDSAFFDKRSKFLHYRPDIAIINNVEFDHADIFDSLDDIKKSFRLLVNLVPSNGLLIVNADDPNSMEVSAKAFC
RVETFGLNGNAEWTAADIATDTDGTSFTVVRDSEAIGRVKVPLFGNYNVMNALAATAAAIRAGVSFESVTRGLCSFKRPK
RRMELVGEYAGGITLIEDFAHHPTAIRLTLGAIAEHYPGRRIIACFEPRSNTTSRNIFQHELSECFGDAAIVVLGKVNRP
ERYAPEERLDAALLCRELKANGKRVFAAGSENYPEDIVRFIEAEQQPGDVVVLLSNGSFSGLKEMLAESFQKNS
>Mature_473_residues
SSIYFIGIGGSAMASVAVALSHMGHAVTGSDTQLYPPMSTYLENHNIRYFNSFSAENLKSATPDLVVVGNAISRGNPDLE
YALDQHMELISMPQLVRRELIGRHTSIVVAGTHGKTTTTSLVAWLLEAGGLLPGFLIGGIPENFGDGCRPSGLSEPGFFV
TEGDEYDSAFFDKRSKFLHYRPDIAIINNVEFDHADIFDSLDDIKKSFRLLVNLVPSNGLLIVNADDPNSMEVSAKAFCR
VETFGLNGNAEWTAADIATDTDGTSFTVVRDSEAIGRVKVPLFGNYNVMNALAATAAAIRAGVSFESVTRGLCSFKRPKR
RMELVGEYAGGITLIEDFAHHPTAIRLTLGAIAEHYPGRRIIACFEPRSNTTSRNIFQHELSECFGDAAIVVLGKVNRPE
RYAPEERLDAALLCRELKANGKRVFAAGSENYPEDIVRFIEAEQQPGDVVVLLSNGSFSGLKEMLAESFQKNS

Specific function: Reutilizes the intact tripeptide L-alanyl-gamma-D- glutamyl-meso-diaminopimelate by linking it to UDP-N-acetylmuramic acid [H]

COG id: COG0773

COG function: function code M; UDP-N-acetylmuramate-alanine ligase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1790680, Length=472, Percent_Identity=40.2542372881356, Blast_Score=327, Evalue=1e-90,
Organism=Escherichia coli, GI1786279, Length=430, Percent_Identity=29.7674418604651, Blast_Score=147, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005757
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 51628; Mature: 51497

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS00697 DNA_LIGASE_A1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSIYFIGIGGSAMASVAVALSHMGHAVTGSDTQLYPPMSTYLENHNIRYFNSFSAENLK
CCEEEEEEECHHHHHHHHHHHHHCCCEECCCCCEECCCHHHHHCCCCEEEEECCCCCHHH
SATPDLVVVGNAISRGNPDLEYALDQHMELISMPQLVRRELIGRHTSIVVAGTHGKTTTT
CCCCCEEEEECHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHH
SLVAWLLEAGGLLPGFLIGGIPENFGDGCRPSGLSEPGFFVTEGDEYDSAFFDKRSKFLH
HHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHCCEE
YRPDIAIINNVEFDHADIFDSLDDIKKSFRLLVNLVPSNGLLIVNADDPNSMEVSAKAFC
ECCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHEECCCCCEEEEECCCCCCEEECEEEEE
RVETFGLNGNAEWTAADIATDTDGTSFTVVRDSEAIGRVKVPLFGNYNVMNALAATAAAI
EEEEECCCCCCCEEEEEEEECCCCCEEEEEECCCCCCEEEEEEECCCHHHHHHHHHHHHH
RAGVSFESVTRGLCSFKRPKRRMELVGEYAGGITLIEDFAHHPTAIRLTLGAIAEHYPGR
HHCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHCCCCCEEEEEHHHHHHHCCCC
RIIACFEPRSNTTSRNIFQHELSECFGDAAIVVLGKVNRPERYAPEERLDAALLCRELKA
EEEEEECCCCCCCHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHC
NGKRVFAAGSENYPEDIVRFIEAEQQPGDVVVLLSNGSFSGLKEMLAESFQKNS
CCCEEEEECCCCCHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SSIYFIGIGGSAMASVAVALSHMGHAVTGSDTQLYPPMSTYLENHNIRYFNSFSAENLK
CEEEEEEECHHHHHHHHHHHHHCCCEECCCCCEECCCHHHHHCCCCEEEEECCCCCHHH
SATPDLVVVGNAISRGNPDLEYALDQHMELISMPQLVRRELIGRHTSIVVAGTHGKTTTT
CCCCCEEEEECHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHH
SLVAWLLEAGGLLPGFLIGGIPENFGDGCRPSGLSEPGFFVTEGDEYDSAFFDKRSKFLH
HHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHCCEE
YRPDIAIINNVEFDHADIFDSLDDIKKSFRLLVNLVPSNGLLIVNADDPNSMEVSAKAFC
ECCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHEECCCCCEEEEECCCCCCEEECEEEEE
RVETFGLNGNAEWTAADIATDTDGTSFTVVRDSEAIGRVKVPLFGNYNVMNALAATAAAI
EEEEECCCCCCCEEEEEEEECCCCCEEEEEECCCCCCEEEEEEECCCHHHHHHHHHHHHH
RAGVSFESVTRGLCSFKRPKRRMELVGEYAGGITLIEDFAHHPTAIRLTLGAIAEHYPGR
HHCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHCCCCCEEEEEHHHHHHHCCCC
RIIACFEPRSNTTSRNIFQHELSECFGDAAIVVLGKVNRPERYAPEERLDAALLCRELKA
EEEEEECCCCCCCHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHC
NGKRVFAAGSENYPEDIVRFIEAEQQPGDVVVLLSNGSFSGLKEMLAESFQKNS
CCCEEEEECCCCCHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 2843822; 7984428; 8808921 [H]