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The map label for this gene is proA

Identifier: 21674293

GI number: 21674293

Start: 1379795

End: 1381057

Strand: Direct

Name: proA

Synonym: CT1473

Alternate gene names: 21674293

Gene position: 1379795-1381057 (Clockwise)

Preceding gene: 21674290

Following gene: 21674294

Centisome position: 64.03

GC content: 61.84

Gene sequence:

>1263_bases
ATGACCGAACACGAAGCTATCGTCAAACAGTTGCAGGCCGTACAAAACGCAAGCCGCAAAATCGTCCCCCTCAATGAAGA
GACCATCAACGGCCTGCTCGTCGAGCTTGCCGACCGCATTCCCGCAGCCGCCGATGCGATCCTCGAAGCCAACCGCAAGG
ACCTCGAACGCATGGATCCCGCCGATCCGCGCTACGATCGGCTGCTCCTGAACGAAGCTCGCCTGAACTCGATCGCCACC
GACCTGCGAAACGTCGCCGCCCTGCCTTCGCCGCTTCACCGGGTGCTCGAAGAGCGCACGCTGCCCAATGGGCTTGAGCT
GAAAAAGGTCTCGGTGCCGCTCGGCGTGATCGGCATCATCTACGAATCGCGCCCGAACGTCACCTTCGACGTCTTCGCGC
TCTGCCTCAAGTCAGGCAACGCCACCGTGCTGAAAGGCGGCAGCGACGCGGCCTACTCGAACATCGCCATCGTGAACCTG
ATACAAACAGTGATCCGCGACCGGGGGCTTGATCCCGATATGATCTACCTCCTGCCCGCCGAGCGCGAGGCGGCGCACAT
TCTGCTCAACGCGGTGGGTTACATCGACGTCATCATTCCACGTGGCAGCCAGGCGCTGATCGACTTCGCGCGCAAGCACT
CCACGGTGCCGGTCATCGAAACCGGAGCGGGCATCGTACACACCTACTTCGACCAGAGCGGCGACCTTGCGATGGGCCGC
GACATTGTTTTCAACGCCAAGACCCGCCGTCCGAGCGTCTGCAATGCGCTCGACACACTGATCGTGCACGAGAGCCGCAT
CGACGACCTGCCGGTGCTGGTGGTGCCGCTCGAAGAGAAAAACGTACAAATTTTCGCCGACGAACCGGCCTATTACAAGC
TGCTTGGCCGCTACCCCGACGAGCTGCTCGAAATGGCTAGCCCGGAGCACTTCGGCACGGAGTTCCTGTCACTGAAGATG
TCGATCAAGACCGTTGGCAGCCTCGAAGAGGCGCTCAACCACATCGCCCGCCACAGTTCGAAGCACAGCGAGGCGATCAT
TGCATCGGATCAGACGACCATCGACGCCTTCATGAAGCAAGTCGATGCGGCGGCGGTGTACGCCAACACCTCGACCGCTT
TCACCGACGGCGCGCAGTTCGGCCTTGGTGCGGAGATCGGCATCAGCACGCAGAAGCTGCACGCGCGCGGCCCAATGGCG
CTCAAGGAGCTGTGCAGCTACAAGTGGCTCGTCACCGGCCAGGGACAGGTGAGGACCGCCTGA

Upstream 100 bases:

>100_bases
AGATCATTCCGCGGCAAACGCCATTGCGCCGGTTGCCGGATATTGACTATCTTTTGCGTCAACGATCACTCACCAATCCC
TTTTTCAAGGAGCAGCGTCC

Downstream 100 bases:

>100_bases
TGCTCGAAGCGCGCAAGCTCGTCAAATCGTACGCCCTGCCCGGCCAGCCACCGCTGAAGATTCTCGACGGCATCGACCTC
TCGGTCGCACCGGGCGAAAT

Product: gamma-glutamyl phosphate reductase

Products: NA

Alternate protein names: GPR; Glutamate-5-semialdehyde dehydrogenase; Glutamyl-gamma-semialdehyde dehydrogenase; GSA dehydrogenase

Number of amino acids: Translated: 420; Mature: 419

Protein sequence:

>420_residues
MTEHEAIVKQLQAVQNASRKIVPLNEETINGLLVELADRIPAAADAILEANRKDLERMDPADPRYDRLLLNEARLNSIAT
DLRNVAALPSPLHRVLEERTLPNGLELKKVSVPLGVIGIIYESRPNVTFDVFALCLKSGNATVLKGGSDAAYSNIAIVNL
IQTVIRDRGLDPDMIYLLPAEREAAHILLNAVGYIDVIIPRGSQALIDFARKHSTVPVIETGAGIVHTYFDQSGDLAMGR
DIVFNAKTRRPSVCNALDTLIVHESRIDDLPVLVVPLEEKNVQIFADEPAYYKLLGRYPDELLEMASPEHFGTEFLSLKM
SIKTVGSLEEALNHIARHSSKHSEAIIASDQTTIDAFMKQVDAAAVYANTSTAFTDGAQFGLGAEIGISTQKLHARGPMA
LKELCSYKWLVTGQGQVRTA

Sequences:

>Translated_420_residues
MTEHEAIVKQLQAVQNASRKIVPLNEETINGLLVELADRIPAAADAILEANRKDLERMDPADPRYDRLLLNEARLNSIAT
DLRNVAALPSPLHRVLEERTLPNGLELKKVSVPLGVIGIIYESRPNVTFDVFALCLKSGNATVLKGGSDAAYSNIAIVNL
IQTVIRDRGLDPDMIYLLPAEREAAHILLNAVGYIDVIIPRGSQALIDFARKHSTVPVIETGAGIVHTYFDQSGDLAMGR
DIVFNAKTRRPSVCNALDTLIVHESRIDDLPVLVVPLEEKNVQIFADEPAYYKLLGRYPDELLEMASPEHFGTEFLSLKM
SIKTVGSLEEALNHIARHSSKHSEAIIASDQTTIDAFMKQVDAAAVYANTSTAFTDGAQFGLGAEIGISTQKLHARGPMA
LKELCSYKWLVTGQGQVRTA
>Mature_419_residues
TEHEAIVKQLQAVQNASRKIVPLNEETINGLLVELADRIPAAADAILEANRKDLERMDPADPRYDRLLLNEARLNSIATD
LRNVAALPSPLHRVLEERTLPNGLELKKVSVPLGVIGIIYESRPNVTFDVFALCLKSGNATVLKGGSDAAYSNIAIVNLI
QTVIRDRGLDPDMIYLLPAEREAAHILLNAVGYIDVIIPRGSQALIDFARKHSTVPVIETGAGIVHTYFDQSGDLAMGRD
IVFNAKTRRPSVCNALDTLIVHESRIDDLPVLVVPLEEKNVQIFADEPAYYKLLGRYPDELLEMASPEHFGTEFLSLKMS
IKTVGSLEEALNHIARHSSKHSEAIIASDQTTIDAFMKQVDAAAVYANTSTAFTDGAQFGLGAEIGISTQKLHARGPMAL
KELCSYKWLVTGQGQVRTA

Specific function: Catalyzes the NADPH dependent reduction of L-gamma- glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate

COG id: COG0014

COG function: function code E; Gamma-glutamyl phosphate reductase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gamma-glutamyl phosphate reductase family

Homologues:

Organism=Homo sapiens, GI62912457, Length=415, Percent_Identity=35.6626506024096, Blast_Score=239, Evalue=3e-63,
Organism=Homo sapiens, GI21361368, Length=415, Percent_Identity=35.6626506024096, Blast_Score=239, Evalue=3e-63,
Organism=Escherichia coli, GI1786438, Length=416, Percent_Identity=41.5865384615385, Blast_Score=307, Evalue=7e-85,
Organism=Caenorhabditis elegans, GI17569967, Length=413, Percent_Identity=33.6561743341404, Blast_Score=223, Evalue=2e-58,
Organism=Caenorhabditis elegans, GI17569969, Length=413, Percent_Identity=33.6561743341404, Blast_Score=222, Evalue=2e-58,
Organism=Saccharomyces cerevisiae, GI6324899, Length=435, Percent_Identity=34.7126436781609, Blast_Score=239, Evalue=7e-64,
Organism=Drosophila melanogaster, GI21357643, Length=415, Percent_Identity=34.9397590361446, Blast_Score=226, Evalue=2e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROA_CHLTE (Q8KCE9)

Other databases:

- EMBL:   AE006470
- RefSeq:   NP_662358.1
- ProteinModelPortal:   Q8KCE9
- SMR:   Q8KCE9
- GeneID:   1006019
- GenomeReviews:   AE006470_GR
- KEGG:   cte:CT1473
- NMPDR:   fig|194439.1.peg.1452
- TIGR:   CT1473
- HOGENOM:   HBG318080
- OMA:   HRIEAMA
- ProtClustDB:   PRK00197
- BioCyc:   CTEP194439:CT_1473-MONOMER
- BRENDA:   1.2.1.41
- GO:   GO:0005737
- HAMAP:   MF_00412
- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR000965
- InterPro:   IPR020593
- InterPro:   IPR012134
- Gene3D:   G3DSA:3.40.309.10
- Gene3D:   G3DSA:3.40.605.10
- PANTHER:   PTHR11063:SF1
- PIRSF:   PIRSF000151
- TIGRFAMs:   TIGR00407

Pfam domain/function: SSF53720 Aldehyde_DH/Histidinol_DH

EC number: =1.2.1.41

Molecular weight: Translated: 45961; Mature: 45830

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS01223 PROA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEHEAIVKQLQAVQNASRKIVPLNEETINGLLVELADRIPAAADAILEANRKDLERMDP
CCHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHCCC
ADPRYDRLLLNEARLNSIATDLRNVAALPSPLHRVLEERTLPNGLELKKVSVPLGVIGII
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEEECCEEEEEEE
YESRPNVTFDVFALCLKSGNATVLKGGSDAAYSNIAIVNLIQTVIRDRGLDPDMIYLLPA
EECCCCCHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEC
EREAAHILLNAVGYIDVIIPRGSQALIDFARKHSTVPVIETGAGIVHTYFDQSGDLAMGR
CCHHHHHHHHHHCEEEEEECCCCHHHHHHHHHCCCCCEEECCCCEEEEEECCCCCEEECC
DIVFNAKTRRPSVCNALDTLIVHESRIDDLPVLVVPLEEKNVQIFADEPAYYKLLGRYPD
EEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEECCCHHHHHHHCCCH
ELLEMASPEHFGTEFLSLKMSIKTVGSLEEALNHIARHSSKHSEAIIASDQTTIDAFMKQ
HHHHHCCCCHHCCHHHEEEEHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHH
VDAAAVYANTSTAFTDGAQFGLGAEIGISTQKLHARGPMALKELCSYKWLVTGQGQVRTA
HHHHEEEECCCCCCCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHCCEEEEECCCCEECC
>Mature Secondary Structure 
TEHEAIVKQLQAVQNASRKIVPLNEETINGLLVELADRIPAAADAILEANRKDLERMDP
CHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHCCC
ADPRYDRLLLNEARLNSIATDLRNVAALPSPLHRVLEERTLPNGLELKKVSVPLGVIGII
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEEECCEEEEEEE
YESRPNVTFDVFALCLKSGNATVLKGGSDAAYSNIAIVNLIQTVIRDRGLDPDMIYLLPA
EECCCCCHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEC
EREAAHILLNAVGYIDVIIPRGSQALIDFARKHSTVPVIETGAGIVHTYFDQSGDLAMGR
CCHHHHHHHHHHCEEEEEECCCCHHHHHHHHHCCCCCEEECCCCEEEEEECCCCCEEECC
DIVFNAKTRRPSVCNALDTLIVHESRIDDLPVLVVPLEEKNVQIFADEPAYYKLLGRYPD
EEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEECCCHHHHHHHCCCH
ELLEMASPEHFGTEFLSLKMSIKTVGSLEEALNHIARHSSKHSEAIIASDQTTIDAFMKQ
HHHHHCCCCHHCCHHHEEEEHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHH
VDAAAVYANTSTAFTDGAQFGLGAEIGISTQKLHARGPMALKELCSYKWLVTGQGQVRTA
HHHHEEEECCCCCCCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHCCEEEEECCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12093901