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The map label for this gene is tpiA

Identifier: 21674265

GI number: 21674265

Start: 1356164

End: 1356901

Strand: Reverse

Name: tpiA

Synonym: CT1444

Alternate gene names: 21674265

Gene position: 1356901-1356164 (Counterclockwise)

Preceding gene: 21674266

Following gene: 21674264

Centisome position: 62.97

GC content: 59.49

Gene sequence:

>738_bases
GTGGTGGGCAACTGGAAGATGAACAACACCATCGCCGAGTCTGTCGATCTGGCAACTGCGATTGCCGAGAAGGTCGGAGC
TGACGGTGTGCAGTGTGAAGTGGGCATCGCCCCCACATTTCCGGCGTTGTACGAGGTTTGCAAGGTGATCGAGTGGAGCG
GAATCAGGCTCTGCGCTCAGAACTGTCATTACGAGAGTGATGGCCCATTCACCGGCGAGGTCTCCACGCGGATGCTTGCC
GCTGCCGGATGCAGCTATGTTATCCTCGGGCACTCGGAGCGCCGTCAACTGTTCGGCGAAACCAATGCCACGGTCAACCT
CAAGGTCAAAAAGGCGTTGGCTGAAGGGTTGAGCGTCATCCTCTGCGTTGGTGAGACGCTCGACGAGCGCGAGCGCGGCG
TGACGGGTCAGATCGTCACTGCCCAAGTGGTTGAGGGGCTGATTGATGTTACCGACATCAGCAAGCTGGTGATTGCCTAC
GAACCGGTATGGGCAATCGGCACCGGCAAAACCGCCACCAAAGAGCAGGCGCAGGAGGTTCACGCGCTGATTCGGGCGAA
AGTCACCGAACTCTACGGTCAGAAAGCGGCGGACCATCTCAGGATCCAGTACGGCGGCAGCGTCAAACCCTCTAACGCTG
CGGAGCTTTTCGCCATGCCGGATATCGACGGTGGCCTGATTGGTGGCGCGAGCCTCAATGCCGACGACTTCATGGCGATT
GTCGAGGCGGCGGGGTAA

Upstream 100 bases:

>100_bases
CTCAGAGATTCCCGTTTCTGGAAGCACACCTTCAAGGTGGCCGATATCGACAAAATCTACGGCGAAGTCGTAACCGATTA
CTGATATGCGCAGAAAAATT

Downstream 100 bases:

>100_bases
AAAGCAAGAGGTTGGAAATTTAGCATTGAAAGGGCTGCTCAGTGATTGGGCAGCCTTTTCTTTTTTTGTGGAGAAATCGA
AGAATAGGTTTGATAAGTTC

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MVGNWKMNNTIAESVDLATAIAEKVGADGVQCEVGIAPTFPALYEVCKVIEWSGIRLCAQNCHYESDGPFTGEVSTRMLA
AAGCSYVILGHSERRQLFGETNATVNLKVKKALAEGLSVILCVGETLDERERGVTGQIVTAQVVEGLIDVTDISKLVIAY
EPVWAIGTGKTATKEQAQEVHALIRAKVTELYGQKAADHLRIQYGGSVKPSNAAELFAMPDIDGGLIGGASLNADDFMAI
VEAAG

Sequences:

>Translated_245_residues
MVGNWKMNNTIAESVDLATAIAEKVGADGVQCEVGIAPTFPALYEVCKVIEWSGIRLCAQNCHYESDGPFTGEVSTRMLA
AAGCSYVILGHSERRQLFGETNATVNLKVKKALAEGLSVILCVGETLDERERGVTGQIVTAQVVEGLIDVTDISKLVIAY
EPVWAIGTGKTATKEQAQEVHALIRAKVTELYGQKAADHLRIQYGGSVKPSNAAELFAMPDIDGGLIGGASLNADDFMAI
VEAAG
>Mature_245_residues
MVGNWKMNNTIAESVDLATAIAEKVGADGVQCEVGIAPTFPALYEVCKVIEWSGIRLCAQNCHYESDGPFTGEVSTRMLA
AAGCSYVILGHSERRQLFGETNATVNLKVKKALAEGLSVILCVGETLDERERGVTGQIVTAQVVEGLIDVTDISKLVIAY
EPVWAIGTGKTATKEQAQEVHALIRAKVTELYGQKAADHLRIQYGGSVKPSNAAELFAMPDIDGGLIGGASLNADDFMAI
VEAAG

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI226529917, Length=243, Percent_Identity=47.3251028806584, Blast_Score=192, Evalue=3e-49,
Organism=Homo sapiens, GI4507645, Length=243, Percent_Identity=47.3251028806584, Blast_Score=191, Evalue=4e-49,
Organism=Escherichia coli, GI1790353, Length=250, Percent_Identity=44.4, Blast_Score=178, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI17536593, Length=243, Percent_Identity=47.3251028806584, Blast_Score=196, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6320255, Length=247, Percent_Identity=41.7004048582996, Blast_Score=169, Evalue=5e-43,
Organism=Drosophila melanogaster, GI28572004, Length=242, Percent_Identity=44.6280991735537, Blast_Score=196, Evalue=2e-50,
Organism=Drosophila melanogaster, GI28572008, Length=242, Percent_Identity=44.6280991735537, Blast_Score=195, Evalue=2e-50,
Organism=Drosophila melanogaster, GI28572006, Length=242, Percent_Identity=44.6280991735537, Blast_Score=195, Evalue=2e-50,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_CHLTE (Q8KCH7)

Other databases:

- EMBL:   AE006470
- RefSeq:   NP_662330.1
- ProteinModelPortal:   Q8KCH7
- SMR:   Q8KCH7
- GeneID:   1005992
- GenomeReviews:   AE006470_GR
- KEGG:   cte:CT1444
- NMPDR:   fig|194439.1.peg.1424
- TIGR:   CT1444
- HOGENOM:   HBG708281
- OMA:   DIRSVQT
- ProtClustDB:   PRK00042
- BioCyc:   CTEP194439:CT_1444-MONOMER
- BRENDA:   5.3.1.1
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 25957; Mature: 25957

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 91-91 ACT_SITE 161-161 BINDING 4-4 BINDING 6-6

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVGNWKMNNTIAESVDLATAIAEKVGADGVQCEVGIAPTFPALYEVCKVIEWSGIRLCAQ
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCCHHHHHH
NCHYESDGPFTGEVSTRMLAAAGCSYVILGHSERRQLFGETNATVNLKVKKALAEGLSVI
HCCCCCCCCCCCHHHHHHHHHCCCCEEEECCCHHHHHCCCCCCEEEEEEHHHHHCCCEEE
LCVGETLDERERGVTGQIVTAQVVEGLIDVTDISKLVIAYEPVWAIGTGKTATKEQAQEV
EEECCCHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHEEECCEEEECCCCCCHHHHHHHH
HALIRAKVTELYGQKAADHLRIQYGGSVKPSNAAELFAMPDIDGGLIGGASLNADDFMAI
HHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCEEEEECCCCCCCEECCCCCCHHHHHHH
VEAAG
HCCCC
>Mature Secondary Structure
MVGNWKMNNTIAESVDLATAIAEKVGADGVQCEVGIAPTFPALYEVCKVIEWSGIRLCAQ
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCCHHHHHH
NCHYESDGPFTGEVSTRMLAAAGCSYVILGHSERRQLFGETNATVNLKVKKALAEGLSVI
HCCCCCCCCCCCHHHHHHHHHCCCCEEEECCCHHHHHCCCCCCEEEEEEHHHHHCCCEEE
LCVGETLDERERGVTGQIVTAQVVEGLIDVTDISKLVIAYEPVWAIGTGKTATKEQAQEV
EEECCCHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHEEECCEEEECCCCCCHHHHHHHH
HALIRAKVTELYGQKAADHLRIQYGGSVKPSNAAELFAMPDIDGGLIGGASLNADDFMAI
HHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCEEEEECCCCCCCEECCCCCCHHHHHHH
VEAAG
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12093901