Click here to switch to the map view.

The map label for this gene is thiC

Identifier: 21674263

GI number: 21674263

Start: 1352637

End: 1354307

Strand: Direct

Name: thiC

Synonym: CT1442

Alternate gene names: 21674263

Gene position: 1352637-1354307 (Clockwise)

Preceding gene: 21674260

Following gene: 21674270

Centisome position: 62.77

GC content: 64.21

Gene sequence:

>1671_bases
ATGAACCAAGAGAACGCATCCTGCCCCAAAAAACACTTTTTCGGCCCTGCATCATCCCGAATCACCGTCAAAGGCACCAT
CTACCCGATCGAGGTCGGCATGAGGCGCGTCGCCCTCACCCGCAGCTACGAGTGCAAGGGCGAGCGTTTCGACGCGATGC
CGCTCTATGACACCAGCGGTCCGTTCGGCGACGCAGAGCGCGAGCACGACGTGCGTAAAGGACTGGAACCGGTGCGCGAC
CGCTGGGGCTTCGACCGCGGCACGGTGGAGTCAGTGGGTGGCGAACTTTCGATGACAGGACGCAAGCCGCGCGTGGCGAA
GGCGGGCGAGGCGGTGACGCAGATGCACTTCGCCCGCAAGGGGATCGTGACGCCGGAGATGGAGTACGTCGCCATCCGCG
AGAACCAGGCGCTCGAAGCGTGGATCGAAAAATGCGGCGGCAAACCGGTGACGCCGGAGATGGTGCGAAGCGAGGTGGCG
CGGGGACGGGCGATCATCCCAGCCAACATCAACCACCCGGAGATCGAACCGATGATCATCGGGCGCAACTTCCGGGTCAA
GATCAACGCCAACATCGGCAACTCGGCGCTTGGTTCGTCGATTGACGAAGAGGTGGAAAAAGCGGTCTGGTCGTGCCGCT
GGGGGGCGGACACGGTGATGGATCTCAGCACCGGCAAGAACATCCACCAGACCCGCGAGTGGATTCTGCGCAACTCGCCC
GTGCCGATCGGCACGGTGCCTCTCTACCAGGCACTCGAAAAGGTTGGCGGCAAGGCCGAGGAGCTGAGCTGGGAGGTCTA
CCGCGACACGCTCGTCGAGCAGGCCGAGCAAGGGGTAGACTACTTCACCATCCACTCGGGCATCCTCGCCGCCACCTTGC
CTGACGCCGAAGCGCGGCAGACCGGCATCGTTTCACGCGGCGGCTCGATCATGGCTCGCTGGTGCCGCGCCCACAAGCAG
GAGAACTTCCTCTTCACCCGCTTTGACGACATCTGCGACATCCTGCGCAGCTACGACGTCGCCATCTCGCTCGGCGACGC
GCTCCGTCCTGGATCGATTGGTGACGCCAACGACGCCGCGCAGTTCGGCGAGCTGAAGACACTCGGTGAGCTGACCCTCC
GCGCCTGGAAGCGCGACGTGCAGGTGATGATCGAAGGCCCCGGCCACGTGCCGCTACACATGATCCGCGAGAACATGGAG
ATGCAGCTCAAGCACTGCCACGAAGCGCCGTTCTATACCCTCGGCCCGCTCGTGACTGACGTGGCCGCCGGATACGACCA
TGTCAATTCGGCCATCGGCGGCACGCTCATCGCCAGCCTCGGCTGCTCGATGCTCTGCTACGTCACGCCGAAAGAGCACC
TCGGCCTACCCAACCGCGATGACGTGCGCGAAGGCGTCATCGTTCACCGCGTCGCCGCCCATGCCGCCGACATCGCCAAG
GGAAGCGCCACCGCCTGGCTGCGCGACGAGCTGATGAGCAAGGCCCGCTACGCCTTCGCGTGGGAAGACCAGTTCAGCCT
CGCCCTCGACCCGCTCAAGACGCGGCAAATCCACGCCCAGAACATCGCCGCCACCGGCGACACCTCCGCCACTGCAAAGT
ATTGCACCATGTGCGGCCCCGACTTCTGCTCGATGAAAAGGTCACAGGAAACGACTGCCGCGGGGTTGTAA

Upstream 100 bases:

>100_bases
AGGTAATGCTGACGCAAGGAAAGATGAAGCACGCATCTCCAAATCCTGCTTCACCGTCCCTTCCGACTCCGGATTCCTGC
TTTTCTTTCTGACTCGCTCC

Downstream 100 bases:

>100_bases
ATCGGGGTTCTTCACTTATAAGTCCTATACGACCTATAGGACTTATAAGACCTTAGTCGCACAATTTATTGTGGCTATCA
ACGATTTTGCCGATTGGGCC

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC

Number of amino acids: Translated: 556; Mature: 556

Protein sequence:

>556_residues
MNQENASCPKKHFFGPASSRITVKGTIYPIEVGMRRVALTRSYECKGERFDAMPLYDTSGPFGDAEREHDVRKGLEPVRD
RWGFDRGTVESVGGELSMTGRKPRVAKAGEAVTQMHFARKGIVTPEMEYVAIRENQALEAWIEKCGGKPVTPEMVRSEVA
RGRAIIPANINHPEIEPMIIGRNFRVKINANIGNSALGSSIDEEVEKAVWSCRWGADTVMDLSTGKNIHQTREWILRNSP
VPIGTVPLYQALEKVGGKAEELSWEVYRDTLVEQAEQGVDYFTIHSGILAATLPDAEARQTGIVSRGGSIMARWCRAHKQ
ENFLFTRFDDICDILRSYDVAISLGDALRPGSIGDANDAAQFGELKTLGELTLRAWKRDVQVMIEGPGHVPLHMIRENME
MQLKHCHEAPFYTLGPLVTDVAAGYDHVNSAIGGTLIASLGCSMLCYVTPKEHLGLPNRDDVREGVIVHRVAAHAADIAK
GSATAWLRDELMSKARYAFAWEDQFSLALDPLKTRQIHAQNIAATGDTSATAKYCTMCGPDFCSMKRSQETTAAGL

Sequences:

>Translated_556_residues
MNQENASCPKKHFFGPASSRITVKGTIYPIEVGMRRVALTRSYECKGERFDAMPLYDTSGPFGDAEREHDVRKGLEPVRD
RWGFDRGTVESVGGELSMTGRKPRVAKAGEAVTQMHFARKGIVTPEMEYVAIRENQALEAWIEKCGGKPVTPEMVRSEVA
RGRAIIPANINHPEIEPMIIGRNFRVKINANIGNSALGSSIDEEVEKAVWSCRWGADTVMDLSTGKNIHQTREWILRNSP
VPIGTVPLYQALEKVGGKAEELSWEVYRDTLVEQAEQGVDYFTIHSGILAATLPDAEARQTGIVSRGGSIMARWCRAHKQ
ENFLFTRFDDICDILRSYDVAISLGDALRPGSIGDANDAAQFGELKTLGELTLRAWKRDVQVMIEGPGHVPLHMIRENME
MQLKHCHEAPFYTLGPLVTDVAAGYDHVNSAIGGTLIASLGCSMLCYVTPKEHLGLPNRDDVREGVIVHRVAAHAADIAK
GSATAWLRDELMSKARYAFAWEDQFSLALDPLKTRQIHAQNIAATGDTSATAKYCTMCGPDFCSMKRSQETTAAGL
>Mature_556_residues
MNQENASCPKKHFFGPASSRITVKGTIYPIEVGMRRVALTRSYECKGERFDAMPLYDTSGPFGDAEREHDVRKGLEPVRD
RWGFDRGTVESVGGELSMTGRKPRVAKAGEAVTQMHFARKGIVTPEMEYVAIRENQALEAWIEKCGGKPVTPEMVRSEVA
RGRAIIPANINHPEIEPMIIGRNFRVKINANIGNSALGSSIDEEVEKAVWSCRWGADTVMDLSTGKNIHQTREWILRNSP
VPIGTVPLYQALEKVGGKAEELSWEVYRDTLVEQAEQGVDYFTIHSGILAATLPDAEARQTGIVSRGGSIMARWCRAHKQ
ENFLFTRFDDICDILRSYDVAISLGDALRPGSIGDANDAAQFGELKTLGELTLRAWKRDVQVMIEGPGHVPLHMIRENME
MQLKHCHEAPFYTLGPLVTDVAAGYDHVNSAIGGTLIASLGCSMLCYVTPKEHLGLPNRDDVREGVIVHRVAAHAADIAK
GSATAWLRDELMSKARYAFAWEDQFSLALDPLKTRQIHAQNIAATGDTSATAKYCTMCGPDFCSMKRSQETTAAGL

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family

Homologues:

Organism=Escherichia coli, GI1790427, Length=571, Percent_Identity=58.6690017513135, Blast_Score=676, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIC_CHLTE (Q8KCH9)

Other databases:

- EMBL:   AE006470
- RefSeq:   NP_662328.1
- GeneID:   1005990
- GenomeReviews:   AE006470_GR
- KEGG:   cte:CT1442
- NMPDR:   fig|194439.1.peg.1422
- TIGR:   CT1442
- HOGENOM:   HBG285234
- OMA:   TWELFRD
- ProtClustDB:   PRK09284
- BioCyc:   CTEP194439:CT_1442-MONOMER
- HAMAP:   MF_00089
- InterPro:   IPR002817
- TIGRFAMs:   TIGR00190

Pfam domain/function: PF01964 ThiC

EC number: NA

Molecular weight: Translated: 61332; Mature: 61332

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: NA

Important sites: BINDING 191-191 BINDING 220-220 BINDING 249-249 BINDING 285-285 BINDING 385-385 BINDING 412-412

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQENASCPKKHFFGPASSRITVKGTIYPIEVGMRRVALTRSYECKGERFDAMPLYDTSG
CCCCCCCCCHHHCCCCCCCEEEEEEEEEEHHHCHHHHHHHCCCCCCCCCCCCCCCCCCCC
PFGDAEREHDVRKGLEPVRDRWGFDRGTVESVGGELSMTGRKPRVAKAGEAVTQMHFARK
CCCCCHHHHHHHHCHHHHHHHCCCCCCCHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHC
GIVTPEMEYVAIRENQALEAWIEKCGGKPVTPEMVRSEVARGRAIIPANINHPEIEPMII
CCCCCCCEEEEECCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEECCCCCCCCCEEEE
GRNFRVKINANIGNSALGSSIDEEVEKAVWSCRWGADTVMDLSTGKNIHQTREWILRNSP
CCEEEEEEECCCCCHHHCCHHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHCCCC
VPIGTVPLYQALEKVGGKAEELSWEVYRDTLVEQAEQGVDYFTIHSGILAATLPDAEARQ
CCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCEEEEECCCCHHHH
TGIVSRGGSIMARWCRAHKQENFLFTRFDDICDILRSYDVAISLGDALRPGSIGDANDAA
CCCCCCCHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCHH
QFGELKTLGELTLRAWKRDVQVMIEGPGHVPLHMIRENMEMQLKHCHEAPFYTLGPLVTD
HHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
VAAGYDHVNSAIGGTLIASLGCSMLCYVTPKEHLGLPNRDDVREGVIVHRVAAHAADIAK
HHHCHHHHHHHHHHHHHHHHCCCEEEEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHC
GSATAWLRDELMSKARYAFAWEDQFSLALDPLKTRQIHAQNIAATGDTSATAKYCTMCGP
CCCHHHHHHHHHHHHHEEEEECCCCCEEECCHHHHHHHHHHCCCCCCCCCHHHHHHHCCC
DFCSMKRSQETTAAGL
HHHHCCCCCCHHHCCC
>Mature Secondary Structure
MNQENASCPKKHFFGPASSRITVKGTIYPIEVGMRRVALTRSYECKGERFDAMPLYDTSG
CCCCCCCCCHHHCCCCCCCEEEEEEEEEEHHHCHHHHHHHCCCCCCCCCCCCCCCCCCCC
PFGDAEREHDVRKGLEPVRDRWGFDRGTVESVGGELSMTGRKPRVAKAGEAVTQMHFARK
CCCCCHHHHHHHHCHHHHHHHCCCCCCCHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHC
GIVTPEMEYVAIRENQALEAWIEKCGGKPVTPEMVRSEVARGRAIIPANINHPEIEPMII
CCCCCCCEEEEECCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEECCCCCCCCCEEEE
GRNFRVKINANIGNSALGSSIDEEVEKAVWSCRWGADTVMDLSTGKNIHQTREWILRNSP
CCEEEEEEECCCCCHHHCCHHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHCCCC
VPIGTVPLYQALEKVGGKAEELSWEVYRDTLVEQAEQGVDYFTIHSGILAATLPDAEARQ
CCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCEEEEECCCCHHHH
TGIVSRGGSIMARWCRAHKQENFLFTRFDDICDILRSYDVAISLGDALRPGSIGDANDAA
CCCCCCCHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCHH
QFGELKTLGELTLRAWKRDVQVMIEGPGHVPLHMIRENMEMQLKHCHEAPFYTLGPLVTD
HHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
VAAGYDHVNSAIGGTLIASLGCSMLCYVTPKEHLGLPNRDDVREGVIVHRVAAHAADIAK
HHHCHHHHHHHHHHHHHHHHCCCEEEEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHC
GSATAWLRDELMSKARYAFAWEDQFSLALDPLKTRQIHAQNIAATGDTSATAKYCTMCGP
CCCHHHHHHHHHHHHHEEEEECCCCCEEECCHHHHHHHHHHCCCCCCCCCHHHHHHHCCC
DFCSMKRSQETTAAGL
HHHHCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12093901