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The map label for this gene is metF [H]

Identifier: 21674190

GI number: 21674190

Start: 1287150

End: 1288022

Strand: Direct

Name: metF [H]

Synonym: CT1368

Alternate gene names: 21674190

Gene position: 1287150-1288022 (Clockwise)

Preceding gene: 21674188

Following gene: 21674192

Centisome position: 59.73

GC content: 55.9

Gene sequence:

>873_bases
ATGCTTGTCAAAGAGATTTTCGATGCACGAACCGAACCGGTATTCAGCCTGGAATTTTTCCCCCCGAAAAAGCAGGACGA
CTGGGACAAACTCTTTGAAACCATCTCGAACCTCTTCCCTCTCGATCCCTCGTATGTGAGCGTTACCTATGGCGCGGGCG
GTTCAACGCGAGAACGCACACACAATCTGGTTACGCGGATTCAGCAAGAGACCGGCCTGACGGTGGTTTCGCACCTCACC
TGTATCGGCGCTGAGAAGAGCGAAATCGAAAGCGTCCTGCAAAACTATCGGGAGCACGGCATCACCAACGTGCTGGCCCT
GCGCGGCGACAAACCCGCCGACATCACGACGCTTGAAGAGGCGACCAAAGACTTTCCACACGCCATCGACCTGGTAAAAT
TCATCAAGGAGAATTTCCCGGAAATGGGCATCGGCGTCGCAGGCTTCCCCGAAGGCCACCCCGAAACGCCGAACCGGATG
AAGGAGCTGGAATTCCTCAAAGAGAAGGTTGACGCGGGCGCGGACTACATCGTCACGCAGCTCTTCTTCGACAACCACGA
CTATTTCGACTACGTCGAGCGCTGCGAGCTGGCAGGCATCACCGTGCCGATCATCCCCGGCATCATGCCGATCATGAGCA
AAAAAGGGATGATCCGCATGTGCGAACTCGCCCTCGGCTCAAGAATCCCGTCAAAGCTGTTGCGCAAAGTACTCGAGGCG
GCGGATGACAAAGAGGTCGCCGAAATCGGCGTCGAATGGGCCACCAACCAGGTGCAGGAACTGCTGGATCACAAAGTCAA
GGGCGTCCACTTCTACACTCTCAACCTCTCCGAAGCCACGCTGAAGATTTTCCGGAATCTGAAGCGAGGGTAA

Upstream 100 bases:

>100_bases
ACAATCAGGGGTTTATGACGGTTATTTCAGAAGTTATGCAATCGTATAATTTAGTATATTCCGGTCATTTTCTGTTCAAC
CAGCAAAAATTCACACCCGG

Downstream 100 bases:

>100_bases
GTAATCATGGTTCTTCGCAGAATTTCGTGGGTGGCATGAAGGATGCTCACATGACCATCCCTGATAAACAAGTTTAACAG
TTCAGTCAAAGAAATTTATA

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MLVKEIFDARTEPVFSLEFFPPKKQDDWDKLFETISNLFPLDPSYVSVTYGAGGSTRERTHNLVTRIQQETGLTVVSHLT
CIGAEKSEIESVLQNYREHGITNVLALRGDKPADITTLEEATKDFPHAIDLVKFIKENFPEMGIGVAGFPEGHPETPNRM
KELEFLKEKVDAGADYIVTQLFFDNHDYFDYVERCELAGITVPIIPGIMPIMSKKGMIRMCELALGSRIPSKLLRKVLEA
ADDKEVAEIGVEWATNQVQELLDHKVKGVHFYTLNLSEATLKIFRNLKRG

Sequences:

>Translated_290_residues
MLVKEIFDARTEPVFSLEFFPPKKQDDWDKLFETISNLFPLDPSYVSVTYGAGGSTRERTHNLVTRIQQETGLTVVSHLT
CIGAEKSEIESVLQNYREHGITNVLALRGDKPADITTLEEATKDFPHAIDLVKFIKENFPEMGIGVAGFPEGHPETPNRM
KELEFLKEKVDAGADYIVTQLFFDNHDYFDYVERCELAGITVPIIPGIMPIMSKKGMIRMCELALGSRIPSKLLRKVLEA
ADDKEVAEIGVEWATNQVQELLDHKVKGVHFYTLNLSEATLKIFRNLKRG
>Mature_290_residues
MLVKEIFDARTEPVFSLEFFPPKKQDDWDKLFETISNLFPLDPSYVSVTYGAGGSTRERTHNLVTRIQQETGLTVVSHLT
CIGAEKSEIESVLQNYREHGITNVLALRGDKPADITTLEEATKDFPHAIDLVKFIKENFPEMGIGVAGFPEGHPETPNRM
KELEFLKEKVDAGADYIVTQLFFDNHDYFDYVERCELAGITVPIIPGIMPIMSKKGMIRMCELALGSRIPSKLLRKVLEA
ADDKEVAEIGVEWATNQVQELLDHKVKGVHFYTLNLSEATLKIFRNLKRG

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=281, Percent_Identity=37.3665480427046, Blast_Score=169, Evalue=2e-42,
Organism=Escherichia coli, GI1790377, Length=273, Percent_Identity=38.8278388278388, Blast_Score=183, Evalue=9e-48,
Organism=Caenorhabditis elegans, GI25147436, Length=281, Percent_Identity=32.7402135231317, Blast_Score=149, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI25147441, Length=281, Percent_Identity=32.7402135231317, Blast_Score=149, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6325234, Length=298, Percent_Identity=38.9261744966443, Blast_Score=195, Evalue=6e-51,
Organism=Saccharomyces cerevisiae, GI6321313, Length=284, Percent_Identity=35.2112676056338, Blast_Score=169, Evalue=6e-43,
Organism=Drosophila melanogaster, GI24662658, Length=279, Percent_Identity=25.8064516129032, Blast_Score=83, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 32773; Mature: 32773

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVKEIFDARTEPVFSLEFFPPKKQDDWDKLFETISNLFPLDPSYVSVTYGAGGSTRERT
CCHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHH
HNLVTRIQQETGLTVVSHLTCIGAEKSEIESVLQNYREHGITNVLALRGDKPADITTLEE
HHHHHHHHHHHCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHH
ATKDFPHAIDLVKFIKENFPEMGIGVAGFPEGHPETPNRMKELEFLKEKVDAGADYIVTQ
HHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHH
LFFDNHDYFDYVERCELAGITVPIIPGIMPIMSKKGMIRMCELALGSRIPSKLLRKVLEA
HHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHH
ADDKEVAEIGVEWATNQVQELLDHKVKGVHFYTLNLSEATLKIFRNLKRG
CCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECHHHHHHHHHHHCCC
>Mature Secondary Structure
MLVKEIFDARTEPVFSLEFFPPKKQDDWDKLFETISNLFPLDPSYVSVTYGAGGSTRERT
CCHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHH
HNLVTRIQQETGLTVVSHLTCIGAEKSEIESVLQNYREHGITNVLALRGDKPADITTLEE
HHHHHHHHHHHCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHH
ATKDFPHAIDLVKFIKENFPEMGIGVAGFPEGHPETPNRMKELEFLKEKVDAGADYIVTQ
HHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHH
LFFDNHDYFDYVERCELAGITVPIIPGIMPIMSKKGMIRMCELALGSRIPSKLLRKVLEA
HHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHH
ADDKEVAEIGVEWATNQVQELLDHKVKGVHFYTLNLSEATLKIFRNLKRG
CCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]