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The map label for this gene is ddl

Identifier: 21674167

GI number: 21674167

Start: 1263277

End: 1264371

Strand: Direct

Name: ddl

Synonym: CT1345

Alternate gene names: 21674167

Gene position: 1263277-1264371 (Clockwise)

Preceding gene: 21674166

Following gene: 21674168

Centisome position: 58.62

GC content: 58.17

Gene sequence:

>1095_bases
ATGTCAAAGCAAACCGTCGCCCTCTTCTTTGGGGGCAAGTCCGCCGAACACGAGATTTCGATCATCTCCGCACGATCCAT
CGCAGCGCAGATCGACCGGAACCGGTATGAACTTTCGCCGCTCTACATCGACCGCGACGGCAAGTGGCACGCCAGCGAGT
GCTCTCAGCAGGTACTCGATACCGACATCGCCGCTCTGCTGAGAAGCGGTACACCAGAGTCCGCCGGAAAACGGCTCGAT
GAACTTACGGCAGCGGCTGCCGGAGAGTGCTTCGACTTCGGCTCGTTCCTCAAAAACACCGACGTGGCCTTCATCGCGCT
GCACGGATCGTACGGCGAGGACGGCAAGCTTCAGGGCTGCCTCGACACCTTCGGCATTCCCTACACCGGCTGCGGCCTGA
CGGCTTCGGCGCTGGCGATGGACAAGGTGCTCACCAAGCTCTGCGCAATGAACGCGGGCATCGCAGTGGCGGAGTTCATG
ACCATCACAAGCTGCGCCTATATGGCCAATCCGCTCGAAACCATCGTGGAGATCACGAAGCGCTTTGACTGGCCGCTCTT
CGTCAAACCGGCCAGTCTCGGCTCGTCGGTCGGCATCTCGAAGGTGCGCAATGCCGAGGAGCTTGCCGCTGCGCTGGAAA
ACGCCTGCGGCCTCGACAGCAAGGCGCTCGTCGAAGCCGCCATTTCAGGCCGCGAAATCGAGGTGGCCGTACTCGGCAAC
AGCGATCCGTTGGCCTCCGAACCAGGTGAAATTATCCCCGGCAGTGACTTCTACAGCTACGAAGACAAGTACATCAAAAA
CGAAGCGAAAATTGTCATACCCGCCGATCTGCCGGAGGGAGTCGCCGAGGAAGTCCGAAAAGCTGCGCTGACCGTCTTCA
AGGCGCTCGGATGCGAGGGAATGGCGAGGGTCGATTTCTTCGTCGAAAACGGTACGAACCGCGTCATTCTGAACGAAATC
AACACGATACCCGGCTTTACCGACATCAGCATGTATCCGATGATGATGGCCGCCTCGGGCATCGGTTTTGCCGAACTGGT
TGAAAAACTGCTGCTTTTGGCTTTGGAAAAGCGGTCAATAACACATAAAATCTGA

Upstream 100 bases:

>100_bases
AGAAAAGCCGGACTCGACGTCGTGCAGAACCGCTGCATCAGCGTCGAACACATGCACCTTGTCTCCTGACCCTCATCCGT
TCACCATCAGTACTATCGCT

Downstream 100 bases:

>100_bases
ACTTACAGGATCGAAGCCTCTCCTGATTTCTCCCAAACCAACCTGACTGAAAACCCGGTATGACCAGTGACCCGCACCTC
ATATTTGCCGATGTGTCGCT

Product: D-alanyl-alanine synthetase A

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 364; Mature: 363

Protein sequence:

>364_residues
MSKQTVALFFGGKSAEHEISIISARSIAAQIDRNRYELSPLYIDRDGKWHASECSQQVLDTDIAALLRSGTPESAGKRLD
ELTAAAAGECFDFGSFLKNTDVAFIALHGSYGEDGKLQGCLDTFGIPYTGCGLTASALAMDKVLTKLCAMNAGIAVAEFM
TITSCAYMANPLETIVEITKRFDWPLFVKPASLGSSVGISKVRNAEELAAALENACGLDSKALVEAAISGREIEVAVLGN
SDPLASEPGEIIPGSDFYSYEDKYIKNEAKIVIPADLPEGVAEEVRKAALTVFKALGCEGMARVDFFVENGTNRVILNEI
NTIPGFTDISMYPMMMAASGIGFAELVEKLLLLALEKRSITHKI

Sequences:

>Translated_364_residues
MSKQTVALFFGGKSAEHEISIISARSIAAQIDRNRYELSPLYIDRDGKWHASECSQQVLDTDIAALLRSGTPESAGKRLD
ELTAAAAGECFDFGSFLKNTDVAFIALHGSYGEDGKLQGCLDTFGIPYTGCGLTASALAMDKVLTKLCAMNAGIAVAEFM
TITSCAYMANPLETIVEITKRFDWPLFVKPASLGSSVGISKVRNAEELAAALENACGLDSKALVEAAISGREIEVAVLGN
SDPLASEPGEIIPGSDFYSYEDKYIKNEAKIVIPADLPEGVAEEVRKAALTVFKALGCEGMARVDFFVENGTNRVILNEI
NTIPGFTDISMYPMMMAASGIGFAELVEKLLLLALEKRSITHKI
>Mature_363_residues
SKQTVALFFGGKSAEHEISIISARSIAAQIDRNRYELSPLYIDRDGKWHASECSQQVLDTDIAALLRSGTPESAGKRLDE
LTAAAAGECFDFGSFLKNTDVAFIALHGSYGEDGKLQGCLDTFGIPYTGCGLTASALAMDKVLTKLCAMNAGIAVAEFMT
ITSCAYMANPLETIVEITKRFDWPLFVKPASLGSSVGISKVRNAEELAAALENACGLDSKALVEAAISGREIEVAVLGNS
DPLASEPGEIIPGSDFYSYEDKYIKNEAKIVIPADLPEGVAEEVRKAALTVFKALGCEGMARVDFFVENGTNRVILNEIN
TIPGFTDISMYPMMMAASGIGFAELVEKLLLLALEKRSITHKI

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786579, Length=366, Percent_Identity=44.2622950819672, Blast_Score=291, Evalue=3e-80,
Organism=Escherichia coli, GI1786280, Length=251, Percent_Identity=39.0438247011952, Blast_Score=162, Evalue=4e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_CHLTE (Q8KCR8)

Other databases:

- EMBL:   AE006470
- RefSeq:   NP_662232.1
- ProteinModelPortal:   Q8KCR8
- SMR:   Q8KCR8
- GeneID:   1006715
- GenomeReviews:   AE006470_GR
- KEGG:   cte:CT1345
- NMPDR:   fig|194439.1.peg.1326
- TIGR:   CT1345
- HOGENOM:   HBG427092
- OMA:   FTDISMY
- ProtClustDB:   PRK01966
- BioCyc:   CTEP194439:CT_1345-MONOMER
- BRENDA:   6.3.2.4
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 39033; Mature: 38902

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKQTVALFFGGKSAEHEISIISARSIAAQIDRNRYELSPLYIDRDGKWHASECSQQVLD
CCCCEEEEEECCCCCCCEEEEEEHHHHHHHHCCCEEEECEEEECCCCCCCHHHHHHHHHH
TDIAALLRSGTPESAGKRLDELTAAAAGECFDFGSFLKNTDVAFIALHGSYGEDGKLQGC
HHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEEEEECCCCCCCCCHHH
LDTFGIPYTGCGLTASALAMDKVLTKLCAMNAGIAVAEFMTITSCAYMANPLETIVEITK
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHH
RFDWPLFVKPASLGSSVGISKVRNAEELAAALENACGLDSKALVEAAISGREIEVAVLGN
HCCCCEEEEHHCCCCCCCHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEEECC
SDPLASEPGEIIPGSDFYSYEDKYIKNEAKIVIPADLPEGVAEEVRKAALTVFKALGCEG
CCCCCCCCCCCCCCCCCCCCCHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCC
MARVDFFVENGTNRVILNEINTIPGFTDISMYPMMMAASGIGFAELVEKLLLLALEKRSI
CEEEEEEEECCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
THKI
HCCC
>Mature Secondary Structure 
SKQTVALFFGGKSAEHEISIISARSIAAQIDRNRYELSPLYIDRDGKWHASECSQQVLD
CCCEEEEEECCCCCCCEEEEEEHHHHHHHHCCCEEEECEEEECCCCCCCHHHHHHHHHH
TDIAALLRSGTPESAGKRLDELTAAAAGECFDFGSFLKNTDVAFIALHGSYGEDGKLQGC
HHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEEEEECCCCCCCCCHHH
LDTFGIPYTGCGLTASALAMDKVLTKLCAMNAGIAVAEFMTITSCAYMANPLETIVEITK
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHH
RFDWPLFVKPASLGSSVGISKVRNAEELAAALENACGLDSKALVEAAISGREIEVAVLGN
HCCCCEEEEHHCCCCCCCHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEEECC
SDPLASEPGEIIPGSDFYSYEDKYIKNEAKIVIPADLPEGVAEEVRKAALTVFKALGCEG
CCCCCCCCCCCCCCCCCCCCCHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCC
MARVDFFVENGTNRVILNEINTIPGFTDISMYPMMMAASGIGFAELVEKLLLLALEKRSI
CEEEEEEEECCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
THKI
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12093901