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The map label for this gene is aspS [H]

Identifier: 21674146

GI number: 21674146

Start: 1244210

End: 1246021

Strand: Reverse

Name: aspS [H]

Synonym: CT1323

Alternate gene names: 21674146

Gene position: 1246021-1244210 (Counterclockwise)

Preceding gene: 21674147

Following gene: 21674145

Centisome position: 57.82

GC content: 57.62

Gene sequence:

>1812_bases
ATGAGTAAAGAGCCAACAACGGCGGATGGTTTGCAGAACAGATTCAGAACGCACTATTGTGGCCGGTTGAACCGCAAGTC
GGAAGGCGAGTTGGTCAGGATCGCTGGATGGGTGCATCGCATCCGCGACCACGGCGGCCTGATCTTCATCGATCTTCGCG
ACCATACCGGCATCTGCCAGCTCGTGGTGCTGCCTGAAAACGAAAGCCAGTTCAAGCTTGCTGAAACGCTGCACTCCGAA
TCGGTGATCTCCGCCGAGGGCAAGGTGGTGCTTCGTTCCGACGAAACCGTTAACCCGAGGCTCGCATCGGGCGCTATCGA
GGTGGTCGTCAGCTCAATCCAGATCGAAAGCAACGCTGATCCGCTGCCCTTCCCGGTGGCCGACGACATGCCGACCTCTG
AGGAGCTGCGTCTGAAATACCGCTTTCTCGACCTGCGCCGCGAAAAGCTGCACGAAAACATCATCTTTCGCAGCAAGCTC
ACCGCCGCCGTCCGGAAATATCTTACCGATCTCGATTTCATCGAAATCCAGACGCCGATTCTCACCTCAAGTTCGCCCGA
GGGCGCGCGCGACTTCCTCGTGCCGAGCCGCCTGCATCCCGGCAAGTTTTACGCGCTGCCGCAGGCTCCACAGCAGTTCA
AGCAGCTCCTGATGGTGGCCGGATTCCCGCGCTATTTCCAGATCGCGCCTTGCTTCCGTGACGAGGATGCCCGCGCCGAC
CGCAGCCCCGGCGAGTTCTACCAGATCGATATCGAGATGTCTTTCATCGAGCAGGACGACCTGTTTGTCATTCTTGAGGG
TATGTTCAAGCACCTGGTCGAGAACATGTCGCATAAGCGCATTACGCAGTTCCCCTTCCCGCGCATCAGCTACAAGGATG
TGATGAACCGCTACGGTTCAGACAAGCCTGACCTGCGCATTCCGCTGGAGATTCAGGACGTGACCGAACTGTTCGTCAAC
TCCGGCTTCAAGGTGTTCGCCTCGAACACGGCGGAAGGCTCGTGCGTCAAGGCGATGGTGCTCAAAGGCATGGGCAATGA
GTCGCGCCTCTTCTACGACAAGGCCGAGAAACGCGCCCGCGAGCTTGGTTCGGCGGGTCTGGCCTACATCCAGTTCAAGG
AGGAGGGGCCGAAAGGGCCGGTGGTGAAGTTCCTCTCCGAAGCAGAGATGAATGCGCTCAAGGAGCGACTCGGTATCGTG
ACTGGTGACGTAGTATTTTTCGGCGCGGGCAAGTGGGAGAAGACCTGCAAGATCATGGGCGGCATGAGGAATTACTTCGC
CGACCTCTTCCCGCTCGACAAGGACGAGCTGTCATTCTGCTGGATCGTCGATTTCCCGATGTTCGAGTACAATGAGGAAG
ATAAAAAGATCGACTTCTCGCACAACCCCTTCTCGATGCCGCAGGGCGAGATGGAGGCTCTCGAATCGAAATTCCCGCTC
GACATTCTGGCCTACCAGTACGACATCGTCTGCAACGGCATCGAACTTTCGAGCGGTGCAATCAGGAACCATCGCCCTGA
CATCATGTACAAGGCCTTCGAAATCGCTGGCTACTCGAAAGAGGAGGTTGATGCCCGTTTTGGCCACATGATCGAAGCCT
TCAAGCACGGTGCGCCGCCGCACGGAGGCATCGCTCCCGGCCTCGATCGTCTCGTCATGATCCTGCGCGACGAACAGAAC
ATTCGCGAAGTCATCGCTTTCCCGATGAACCAGTCCGCCCAGGACCTTATGATGGCCGCTCCGTCGGAAGTCACCGCTCA
GCAGCTCAAGGAGCTTTGCATCAGGATCGAACTCCCGGAAGAGGAAAAGTGA

Upstream 100 bases:

>100_bases
GTCGTTTTTATAGAATTCTTGTTCCGGCAGTGATCGGGATTATGACATTTTTTCACTACGAGTCCCCAGGGGCTTTCAAA
ACGGGTTATACACGATTTAT

Downstream 100 bases:

>100_bases
TGGCCAGGGTTAATGGCAGTGTTTGGTTTGAAATGAAAAATCCCGCAAGACGCAAGGTTTTGCGGGATTTTTTTTGCGGG
AAAACTATAACCGTCTGGTA

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS [H]

Number of amino acids: Translated: 603; Mature: 602

Protein sequence:

>603_residues
MSKEPTTADGLQNRFRTHYCGRLNRKSEGELVRIAGWVHRIRDHGGLIFIDLRDHTGICQLVVLPENESQFKLAETLHSE
SVISAEGKVVLRSDETVNPRLASGAIEVVVSSIQIESNADPLPFPVADDMPTSEELRLKYRFLDLRREKLHENIIFRSKL
TAAVRKYLTDLDFIEIQTPILTSSSPEGARDFLVPSRLHPGKFYALPQAPQQFKQLLMVAGFPRYFQIAPCFRDEDARAD
RSPGEFYQIDIEMSFIEQDDLFVILEGMFKHLVENMSHKRITQFPFPRISYKDVMNRYGSDKPDLRIPLEIQDVTELFVN
SGFKVFASNTAEGSCVKAMVLKGMGNESRLFYDKAEKRARELGSAGLAYIQFKEEGPKGPVVKFLSEAEMNALKERLGIV
TGDVVFFGAGKWEKTCKIMGGMRNYFADLFPLDKDELSFCWIVDFPMFEYNEEDKKIDFSHNPFSMPQGEMEALESKFPL
DILAYQYDIVCNGIELSSGAIRNHRPDIMYKAFEIAGYSKEEVDARFGHMIEAFKHGAPPHGGIAPGLDRLVMILRDEQN
IREVIAFPMNQSAQDLMMAAPSEVTAQQLKELCIRIELPEEEK

Sequences:

>Translated_603_residues
MSKEPTTADGLQNRFRTHYCGRLNRKSEGELVRIAGWVHRIRDHGGLIFIDLRDHTGICQLVVLPENESQFKLAETLHSE
SVISAEGKVVLRSDETVNPRLASGAIEVVVSSIQIESNADPLPFPVADDMPTSEELRLKYRFLDLRREKLHENIIFRSKL
TAAVRKYLTDLDFIEIQTPILTSSSPEGARDFLVPSRLHPGKFYALPQAPQQFKQLLMVAGFPRYFQIAPCFRDEDARAD
RSPGEFYQIDIEMSFIEQDDLFVILEGMFKHLVENMSHKRITQFPFPRISYKDVMNRYGSDKPDLRIPLEIQDVTELFVN
SGFKVFASNTAEGSCVKAMVLKGMGNESRLFYDKAEKRARELGSAGLAYIQFKEEGPKGPVVKFLSEAEMNALKERLGIV
TGDVVFFGAGKWEKTCKIMGGMRNYFADLFPLDKDELSFCWIVDFPMFEYNEEDKKIDFSHNPFSMPQGEMEALESKFPL
DILAYQYDIVCNGIELSSGAIRNHRPDIMYKAFEIAGYSKEEVDARFGHMIEAFKHGAPPHGGIAPGLDRLVMILRDEQN
IREVIAFPMNQSAQDLMMAAPSEVTAQQLKELCIRIELPEEEK
>Mature_602_residues
SKEPTTADGLQNRFRTHYCGRLNRKSEGELVRIAGWVHRIRDHGGLIFIDLRDHTGICQLVVLPENESQFKLAETLHSES
VISAEGKVVLRSDETVNPRLASGAIEVVVSSIQIESNADPLPFPVADDMPTSEELRLKYRFLDLRREKLHENIIFRSKLT
AAVRKYLTDLDFIEIQTPILTSSSPEGARDFLVPSRLHPGKFYALPQAPQQFKQLLMVAGFPRYFQIAPCFRDEDARADR
SPGEFYQIDIEMSFIEQDDLFVILEGMFKHLVENMSHKRITQFPFPRISYKDVMNRYGSDKPDLRIPLEIQDVTELFVNS
GFKVFASNTAEGSCVKAMVLKGMGNESRLFYDKAEKRARELGSAGLAYIQFKEEGPKGPVVKFLSEAEMNALKERLGIVT
GDVVFFGAGKWEKTCKIMGGMRNYFADLFPLDKDELSFCWIVDFPMFEYNEEDKKIDFSHNPFSMPQGEMEALESKFPLD
ILAYQYDIVCNGIELSSGAIRNHRPDIMYKAFEIAGYSKEEVDARFGHMIEAFKHGAPPHGGIAPGLDRLVMILRDEQNI
REVIAFPMNQSAQDLMMAAPSEVTAQQLKELCIRIELPEEEK

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI40789249, Length=602, Percent_Identity=36.3787375415282, Blast_Score=369, Evalue=1e-102,
Organism=Homo sapiens, GI45439306, Length=252, Percent_Identity=29.3650793650794, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI226491502, Length=255, Percent_Identity=26.6666666666667, Blast_Score=93, Evalue=6e-19,
Organism=Homo sapiens, GI4758762, Length=284, Percent_Identity=26.7605633802817, Blast_Score=79, Evalue=9e-15,
Organism=Homo sapiens, GI5031815, Length=268, Percent_Identity=26.865671641791, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI194272210, Length=268, Percent_Identity=26.865671641791, Blast_Score=72, Evalue=2e-12,
Organism=Escherichia coli, GI1788173, Length=591, Percent_Identity=42.4703891708968, Blast_Score=455, Evalue=1e-129,
Organism=Escherichia coli, GI1790571, Length=294, Percent_Identity=26.530612244898, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI1789256, Length=294, Percent_Identity=25.1700680272109, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI32566633, Length=601, Percent_Identity=33.6106489184692, Blast_Score=306, Evalue=2e-83,
Organism=Caenorhabditis elegans, GI17551876, Length=282, Percent_Identity=24.113475177305, Blast_Score=77, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71994340, Length=274, Percent_Identity=24.0875912408759, Blast_Score=72, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17535927, Length=274, Percent_Identity=24.0875912408759, Blast_Score=72, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17535925, Length=274, Percent_Identity=24.0875912408759, Blast_Score=72, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6325153, Length=355, Percent_Identity=34.9295774647887, Blast_Score=160, Evalue=6e-40,
Organism=Saccharomyces cerevisiae, GI6323011, Length=296, Percent_Identity=29.0540540540541, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6321807, Length=292, Percent_Identity=25, Blast_Score=78, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6324256, Length=303, Percent_Identity=24.0924092409241, Blast_Score=66, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6320242, Length=317, Percent_Identity=23.9747634069401, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24584738, Length=602, Percent_Identity=34.3853820598007, Blast_Score=317, Evalue=1e-86,
Organism=Drosophila melanogaster, GI17136276, Length=246, Percent_Identity=28.8617886178862, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI19921528, Length=294, Percent_Identity=26.8707482993197, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24640849, Length=300, Percent_Identity=25.6666666666667, Blast_Score=65, Evalue=1e-10,
Organism=Drosophila melanogaster, GI24640851, Length=300, Percent_Identity=25.6666666666667, Blast_Score=65, Evalue=1e-10,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.12 [H]

Molecular weight: Translated: 68599; Mature: 68468

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKEPTTADGLQNRFRTHYCGRLNRKSEGELVRIAGWVHRIRDHGGLIFIDLRDHTGICQ
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHCCCEEEEEECCCCCEEE
LVVLPENESQFKLAETLHSESVISAEGKVVLRSDETVNPRLASGAIEVVVSSIQIESNAD
EEEECCCCCHHHHHHHHCCCCEEECCCEEEEECCCCCCCHHHHHHHHHHHHHEEEECCCC
PLPFPVADDMPTSEELRLKYRFLDLRREKLHENIIFRSKLTAAVRKYLTDLDFIEIQTPI
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCE
LTSSSPEGARDFLVPSRLHPGKFYALPQAPQQFKQLLMVAGFPRYFQIAPCFRDEDARAD
ECCCCCCCCHHCCCCCCCCCCCEEECCCCHHHHHHHHHHHCCCCCEEECCCCCCCCCCCC
RSPGEFYQIDIEMSFIEQDDLFVILEGMFKHLVENMSHKRITQFPFPRISYKDVMNRYGS
CCCCCEEEEEEEEEEECCCCEEEHHHHHHHHHHHHCCCCCHHCCCCCCCCHHHHHHHHCC
DKPDLRIPLEIQDVTELFVNSGFKVFASNTAEGSCVKAMVLKGMGNESRLFYDKAEKRAR
CCCCEEEEEEHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHCCCCCCEEEHHHHHHHHH
ELGSAGLAYIQFKEEGPKGPVVKFLSEAEMNALKERLGIVTGDVVFFGAGKWEKTCKIMG
HHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCEEECCEEEEECCCHHHHHHHHH
GMRNYFADLFPLDKDELSFCWIVDFPMFEYNEEDKKIDFSHNPFSMPQGEMEALESKFPL
HHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCEEECCCCCCCCCCHHHHHHHCCCCE
DILAYQYDIVCNGIELSSGAIRNHRPDIMYKAFEIAGYSKEEVDARFGHMIEAFKHGAPP
EHEEEEEEEEECCEEECCCCCCCCCCCHHEEEHHHCCCCHHHHHHHHHHHHHHHHCCCCC
HGGIAPGLDRLVMILRDEQNIREVIAFPMNQSAQDLMMAAPSEVTAQQLKELCIRIELPE
CCCCCCCHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHCCCCHHHHHHHHHHHEEECCCC
EEK
CCC
>Mature Secondary Structure 
SKEPTTADGLQNRFRTHYCGRLNRKSEGELVRIAGWVHRIRDHGGLIFIDLRDHTGICQ
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHCCCEEEEEECCCCCEEE
LVVLPENESQFKLAETLHSESVISAEGKVVLRSDETVNPRLASGAIEVVVSSIQIESNAD
EEEECCCCCHHHHHHHHCCCCEEECCCEEEEECCCCCCCHHHHHHHHHHHHHEEEECCCC
PLPFPVADDMPTSEELRLKYRFLDLRREKLHENIIFRSKLTAAVRKYLTDLDFIEIQTPI
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCE
LTSSSPEGARDFLVPSRLHPGKFYALPQAPQQFKQLLMVAGFPRYFQIAPCFRDEDARAD
ECCCCCCCCHHCCCCCCCCCCCEEECCCCHHHHHHHHHHHCCCCCEEECCCCCCCCCCCC
RSPGEFYQIDIEMSFIEQDDLFVILEGMFKHLVENMSHKRITQFPFPRISYKDVMNRYGS
CCCCCEEEEEEEEEEECCCCEEEHHHHHHHHHHHHCCCCCHHCCCCCCCCHHHHHHHHCC
DKPDLRIPLEIQDVTELFVNSGFKVFASNTAEGSCVKAMVLKGMGNESRLFYDKAEKRAR
CCCCEEEEEEHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHCCCCCCEEEHHHHHHHHH
ELGSAGLAYIQFKEEGPKGPVVKFLSEAEMNALKERLGIVTGDVVFFGAGKWEKTCKIMG
HHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCEEECCEEEEECCCHHHHHHHHH
GMRNYFADLFPLDKDELSFCWIVDFPMFEYNEEDKKIDFSHNPFSMPQGEMEALESKFPL
HHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCEEECCCCCCCCCCHHHHHHHCCCCE
DILAYQYDIVCNGIELSSGAIRNHRPDIMYKAFEIAGYSKEEVDARFGHMIEAFKHGAPP
EHEEEEEEEEECCEEECCCCCCCCCCCHHEEEHHHCCCCHHHHHHHHHHHHHHHHCCCCC
HGGIAPGLDRLVMILRDEQNIREVIAFPMNQSAQDLMMAAPSEVTAQQLKELCIRIELPE
CCCCCCCHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHCCCCHHHHHHHHHHHEEECCCC
EEK
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA