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The map label for this gene is rhtB [H]

Identifier: 21674090

GI number: 21674090

Start: 1187642

End: 1188265

Strand: Reverse

Name: rhtB [H]

Synonym: CT1267

Alternate gene names: 21674090

Gene position: 1188265-1187642 (Counterclockwise)

Preceding gene: 21674091

Following gene: 21674089

Centisome position: 55.14

GC content: 58.01

Gene sequence:

>624_bases
ATGATCGATTTTCAGACGTTTTTTCTCTTTTTCCCGGTGGCCCTGCTGCTGGCGCTCTCGCCGGGGCCGGACAACCTGTT
TGTGCTTGCGCAATCCGCACAGCATGGCAGACCTGCCGGTTTTGCCGTGACCATTGGGCTTTGCACGGGTCTGATTGGCC
ATACTCTCGCCGTGGCGTTTGGACTTGCGGCTGTCGTCAAGGCATCGGCGCTTGCTTTCACGGTGTTGAAGATTGCAGGA
GCACTTTATCTGCTCTGGCTCGCCTGGCAGGCATGGCGGGCAGGCGGTGAGGTCGGCGAGTCGAACGCGCATGCCCTCAG
CGGGATCGAGCTTTACCGGCGGGGGATGGTGATGAATCTCACCAACCCGAAGGTTTCGCTCTTTTTCCTCGCCTTTCTTC
CCCAGTTTACCGATCCACGCCACGGTTCGATGACCATGCAGTTCATCGAACTGGGGGCCCTCTTCATTCTTGCCACCCTG
ATCGTTTTCGCCGGATTGAGTATGGTTGCCGGTGGGCTTGGAGAGCGCTTCCGCCGTTCACCTGCCGCACTCAGATTGGT
CAACCGCGCCGCTGCCATGATTTTTACCGGACTTGCCATAAAACTGGCCATAACCGAACGCTAA

Upstream 100 bases:

>100_bases
CTGGTTGGCATCGGATCGAGAAATGGGCGGTGCTTCGATGTCATCTATCTCCAGAAGACGTTTTGAATCTTCGGTGAAGA
GCTAAATAAACAGTTTTGCC

Downstream 100 bases:

>100_bases
ATAACCTTCTCTCATCGGTGGTGATTATGGGGGGGGCTTTTTGTTTTTCTGTACAAAAAAACGTACAATAAAAAGTGCGC
TATTTGATACTGTCAATGAT

Product: amino acid efflux protein, putative

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 207; Mature: 207

Protein sequence:

>207_residues
MIDFQTFFLFFPVALLLALSPGPDNLFVLAQSAQHGRPAGFAVTIGLCTGLIGHTLAVAFGLAAVVKASALAFTVLKIAG
ALYLLWLAWQAWRAGGEVGESNAHALSGIELYRRGMVMNLTNPKVSLFFLAFLPQFTDPRHGSMTMQFIELGALFILATL
IVFAGLSMVAGGLGERFRRSPAALRLVNRAAAMIFTGLAIKLAITER

Sequences:

>Translated_207_residues
MIDFQTFFLFFPVALLLALSPGPDNLFVLAQSAQHGRPAGFAVTIGLCTGLIGHTLAVAFGLAAVVKASALAFTVLKIAG
ALYLLWLAWQAWRAGGEVGESNAHALSGIELYRRGMVMNLTNPKVSLFFLAFLPQFTDPRHGSMTMQFIELGALFILATL
IVFAGLSMVAGGLGERFRRSPAALRLVNRAAAMIFTGLAIKLAITER
>Mature_207_residues
MIDFQTFFLFFPVALLLALSPGPDNLFVLAQSAQHGRPAGFAVTIGLCTGLIGHTLAVAFGLAAVVKASALAFTVLKIAG
ALYLLWLAWQAWRAGGEVGESNAHALSGIELYRRGMVMNLTNPKVSLFFLAFLPQFTDPRHGSMTMQFIELGALFILATL
IVFAGLSMVAGGLGERFRRSPAALRLVNRAAAMIFTGLAIKLAITER

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=189, Percent_Identity=34.9206349206349, Blast_Score=106, Evalue=9e-25,
Organism=Escherichia coli, GI1788099, Length=196, Percent_Identity=27.5510204081633, Blast_Score=79, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 22169; Mature: 22169

Theoretical pI: Translated: 10.84; Mature: 10.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDFQTFFLFFPVALLLALSPGPDNLFVLAQSAQHGRPAGFAVTIGLCTGLIGHTLAVAF
CCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCEEHHHHHHHHHHHHHHHHH
GLAAVVKASALAFTVLKIAGALYLLWLAWQAWRAGGEVGESNAHALSGIELYRRGMVMNL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCEEEEE
TNPKVSLFFLAFLPQFTDPRHGSMTMQFIELGALFILATLIVFAGLSMVAGGLGERFRRS
CCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PAALRLVNRAAAMIFTGLAIKLAITER
HHHHHHHHHHHHHHHHHHHEEEEEECC
>Mature Secondary Structure
MIDFQTFFLFFPVALLLALSPGPDNLFVLAQSAQHGRPAGFAVTIGLCTGLIGHTLAVAF
CCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCEEHHHHHHHHHHHHHHHHH
GLAAVVKASALAFTVLKIAGALYLLWLAWQAWRAGGEVGESNAHALSGIELYRRGMVMNL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCEEEEE
TNPKVSLFFLAFLPQFTDPRHGSMTMQFIELGALFILATLIVFAGLSMVAGGLGERFRRS
CCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PAALRLVNRAAAMIFTGLAIKLAITER
HHHHHHHHHHHHHHHHHHHEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]