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The map label for this gene is dacA [H]

Identifier: 21674088

GI number: 21674088

Start: 1185641

End: 1187065

Strand: Reverse

Name: dacA [H]

Synonym: CT1265

Alternate gene names: 21674088

Gene position: 1187065-1185641 (Counterclockwise)

Preceding gene: 21674089

Following gene: 21674087

Centisome position: 55.09

GC content: 54.88

Gene sequence:

>1425_bases
ATGGGGCGGCCGCATGGTCGTTTTTATGCCACAAGAAAAGTGCTTTTTACACTTCTTGCCTTTTTTGTTCTCACGTTGAT
TCCGCTCACCGCTTCTGCCAAAAGGCGGCCAGCCCCGGAGGGCGAAGATGCGGTTGCGGCCTATATCGTCAAGGAGACCG
GCAGTCCGGAATTTTTGAAATCGAAGAATGCTGATACACCGCGTTCGCCTGCGAGCCTGACCAAGATCATGACCTGCCTG
CTGGCTATCGAAAGTGGCCGCATGAATGATGTGGTGACGATTCCTCTTGAGGCGACGCAGGTCGAACCGACGCGAGCCGG
TTTCAAGCCGGGAGAGCAGTTCAGGCTTCGAGATCTGGTGAAGGCTGCCATGGTCAATTCAAGTAACGATGCCGCTTTTG
CCATTGCCATTCACCTCGGTGGCAGTCTCGATGCTTTTGTAGCAATGATGAATGCTCGTGCCCGCGCTCTCGGGATGAGC
CATACCGTCTTCACCAATCCTGCCGGTTACGATCGGGGTATTTATGCTGGTAATCGCACGACGGCAAGGGACCTGGTAAT
TCTGACCGAACGTGCGATCAGGTTTCCAGAATTCAACGCGATTGCAAAGCTGGACAGGGTGGATTTCAACGAGCTTTCCA
CCGGTAAGATTTACAGTCTTCGCACTCACAACAAGCTCCTTGAACGTTACCCCTATTCCGTTGGTATCAAGACCGGTTAT
ACCTCAATGGCAGGCCCATGCCTTATTGCAAGGGCGTTGAGGAATGGCAAGGATATGCTTGTCATTATGCTTTCTGCCCG
GACGGATCGCTGGTCACTGGCGTCTACCATGTTCGATCAGGGTTTCGGGCTTGATGCAGGCCCGGTTCAGGTTGCGGAAA
CTGCTGAGAAATCTCCCCGCAGGGTTGCTGTTGACGCTCCGGAGGCTCCTCATTCAAGCGTGAGCGTCAGGCGCGCCCAG
GCGCTTGAGGCACTGAGGCTCAAGGTCGAGAGCCGCCGCGGCCAATCCGCAGTAACGGAGATTCGCGGAATGAGCATGAA
TGTTCGGTCGTCAGGCGCGGACTCTTCGGTAAAAGCCCGCATGGAAAAGCGGAAAGCTTCTGCGGTCATCAAATTGCGCG
GCAAGTCAAGCCGTGCTGACAGGATAGCGCTCAGGGCTGGCAAGAACGCTTCGGCCAGGAGGAAGCAACAGCTCGCAATG
GCCCGGCAACGTAACCACAAAAACGGGGTCGTCCTGAAGTCTGCAAAGAAAAATGCTTCTGGCAATGTGGCTCTCAAGTC
GCATAACAAGGCTCAGCGCTCTATCAAAACTGCCCGAAAGGGAGCGGTAAAGCGTTCGACTGCCGATGCAAAATCGGCAA
AAAGCCGCAGCTGGAAAGAGGGGCTTTCTCTGTCGGAGAGAGCCGTTCGTTCTCCGAATGGTTGA

Upstream 100 bases:

>100_bases
CCCTCAACGGCTGGAGTGACTGCCAACAGAAGAATGTATCGCCATGTCAGCACGTATGTTAGACTCCTATGAGCCCTGGA
AGCGGGGTGCAGGCAGTGAA

Downstream 100 bases:

>100_bases
GGGGAGCTGCATTTGAGAAGAGTGTTCATCCATACCTTTGAAGTTCCCGGATCGTCGATCGACGGATATGGGCATGTCAG
CAATATCGAATATCTCCGGT

Product: D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5*; PBP-5* [H]

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MGRPHGRFYATRKVLFTLLAFFVLTLIPLTASAKRRPAPEGEDAVAAYIVKETGSPEFLKSKNADTPRSPASLTKIMTCL
LAIESGRMNDVVTIPLEATQVEPTRAGFKPGEQFRLRDLVKAAMVNSSNDAAFAIAIHLGGSLDAFVAMMNARARALGMS
HTVFTNPAGYDRGIYAGNRTTARDLVILTERAIRFPEFNAIAKLDRVDFNELSTGKIYSLRTHNKLLERYPYSVGIKTGY
TSMAGPCLIARALRNGKDMLVIMLSARTDRWSLASTMFDQGFGLDAGPVQVAETAEKSPRRVAVDAPEAPHSSVSVRRAQ
ALEALRLKVESRRGQSAVTEIRGMSMNVRSSGADSSVKARMEKRKASAVIKLRGKSSRADRIALRAGKNASARRKQQLAM
ARQRNHKNGVVLKSAKKNASGNVALKSHNKAQRSIKTARKGAVKRSTADAKSAKSRSWKEGLSLSERAVRSPNG

Sequences:

>Translated_474_residues
MGRPHGRFYATRKVLFTLLAFFVLTLIPLTASAKRRPAPEGEDAVAAYIVKETGSPEFLKSKNADTPRSPASLTKIMTCL
LAIESGRMNDVVTIPLEATQVEPTRAGFKPGEQFRLRDLVKAAMVNSSNDAAFAIAIHLGGSLDAFVAMMNARARALGMS
HTVFTNPAGYDRGIYAGNRTTARDLVILTERAIRFPEFNAIAKLDRVDFNELSTGKIYSLRTHNKLLERYPYSVGIKTGY
TSMAGPCLIARALRNGKDMLVIMLSARTDRWSLASTMFDQGFGLDAGPVQVAETAEKSPRRVAVDAPEAPHSSVSVRRAQ
ALEALRLKVESRRGQSAVTEIRGMSMNVRSSGADSSVKARMEKRKASAVIKLRGKSSRADRIALRAGKNASARRKQQLAM
ARQRNHKNGVVLKSAKKNASGNVALKSHNKAQRSIKTARKGAVKRSTADAKSAKSRSWKEGLSLSERAVRSPNG
>Mature_473_residues
GRPHGRFYATRKVLFTLLAFFVLTLIPLTASAKRRPAPEGEDAVAAYIVKETGSPEFLKSKNADTPRSPASLTKIMTCLL
AIESGRMNDVVTIPLEATQVEPTRAGFKPGEQFRLRDLVKAAMVNSSNDAAFAIAIHLGGSLDAFVAMMNARARALGMSH
TVFTNPAGYDRGIYAGNRTTARDLVILTERAIRFPEFNAIAKLDRVDFNELSTGKIYSLRTHNKLLERYPYSVGIKTGYT
SMAGPCLIARALRNGKDMLVIMLSARTDRWSLASTMFDQGFGLDAGPVQVAETAEKSPRRVAVDAPEAPHSSVSVRRAQA
LEALRLKVESRRGQSAVTEIRGMSMNVRSSGADSSVKARMEKRKASAVIKLRGKSSRADRIALRAGKNASARRKQQLAMA
RQRNHKNGVVLKSAKKNASGNVALKSHNKAQRSIKTARKGAVKRSTADAKSAKSRSWKEGLSLSERAVRSPNG

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors. Required specifically for the synthesis of the spore form of peptidoglycan (cortex) [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Forespore outer membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=262, Percent_Identity=32.824427480916, Blast_Score=111, Evalue=8e-26,
Organism=Escherichia coli, GI1786851, Length=234, Percent_Identity=33.3333333333333, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI87082022, Length=265, Percent_Identity=30.188679245283, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI87082054, Length=239, Percent_Identity=28.4518828451883, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 51596; Mature: 51465

Theoretical pI: Translated: 11.52; Mature: 11.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRPHGRFYATRKVLFTLLAFFVLTLIPLTASAKRRPAPEGEDAVAAYIVKETGSPEFLK
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHH
SKNADTPRSPASLTKIMTCLLAIESGRMNDVVTIPLEATQVEPTRAGFKPGEQFRLRDLV
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHH
KAAMVNSSNDAAFAIAIHLGGSLDAFVAMMNARARALGMSHTVFTNPAGYDRGIYAGNRT
HHHHHCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEECCCCC
TARDLVILTERAIRFPEFNAIAKLDRVDFNELSTGKIYSLRTHNKLLERYPYSVGIKTGY
CHHHEEEEEHHHHCCCCCHHHHHHCCCCCCCCCCCCEEEEHHHHHHHHHCCCEEEECCCC
TSMAGPCLIARALRNGKDMLVIMLSARTDRWSLASTMFDQGFGLDAGPVQVAETAEKSPR
CHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCCHHHHHHHCCCCC
RVAVDAPEAPHSSVSVRRAQALEALRLKVESRRGQSAVTEIRGMSMNVRSSGADSSVKAR
EEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCEEECCCCCCCHHHHHH
MEKRKASAVIKLRGKSSRADRIALRAGKNASARRKQQLAMARQRNHKNGVVLKSAKKNAS
HHHHHHCEEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCC
GNVALKSHNKAQRSIKTARKGAVKRSTADAKSAKSRSWKEGLSLSERAVRSPNG
CCEEEECCCHHHHHHHHHHHCCHHCCCCHHHHHHHCCHHHCCCHHHHHHCCCCC
>Mature Secondary Structure 
GRPHGRFYATRKVLFTLLAFFVLTLIPLTASAKRRPAPEGEDAVAAYIVKETGSPEFLK
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHH
SKNADTPRSPASLTKIMTCLLAIESGRMNDVVTIPLEATQVEPTRAGFKPGEQFRLRDLV
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHH
KAAMVNSSNDAAFAIAIHLGGSLDAFVAMMNARARALGMSHTVFTNPAGYDRGIYAGNRT
HHHHHCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEECCCCC
TARDLVILTERAIRFPEFNAIAKLDRVDFNELSTGKIYSLRTHNKLLERYPYSVGIKTGY
CHHHEEEEEHHHHCCCCCHHHHHHCCCCCCCCCCCCEEEEHHHHHHHHHCCCEEEECCCC
TSMAGPCLIARALRNGKDMLVIMLSARTDRWSLASTMFDQGFGLDAGPVQVAETAEKSPR
CHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCCHHHHHHHCCCCC
RVAVDAPEAPHSSVSVRRAQALEALRLKVESRRGQSAVTEIRGMSMNVRSSGADSSVKAR
EEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCEEECCCCCCCHHHHHH
MEKRKASAVIKLRGKSSRADRIALRAGKNASARRKQQLAMARQRNHKNGVVLKSAKKNAS
HHHHHHCEEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCC
GNVALKSHNKAQRSIKTARKGAVKRSTADAKSAKSRSWKEGLSLSERAVRSPNG
CCEEEECCCHHHHHHHHHHHCCHHCCCCHHHHHHHCCHHHCCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1548223; 7934829; 9384377 [H]