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The map label for this gene is pth

Identifier: 21674075

GI number: 21674075

Start: 1178098

End: 1178661

Strand: Direct

Name: pth

Synonym: CT1252

Alternate gene names: 21674075

Gene position: 1178098-1178661 (Clockwise)

Preceding gene: 21674074

Following gene: 21674080

Centisome position: 54.67

GC content: 56.74

Gene sequence:

>564_bases
ATGAAACTTATCGTCGGCCTTGGCAACCCGGAACTTCGGCACGCGGCAACGAGGCATAATATCGGATTCGACGTCATCGA
CCATCTTGCAGGAAGCTCGACATTCTCATCGGGCAAGGGAAATTACCGGTTCACAAAGATCACCGCACCGGGCGGGCCGC
TGATTCTGGTCAAGCCGATGACCTACATGAACCTTTCGGGTCATGCCGTCGTTGCGGCGATGAATTTCTGGAAGATCGAA
CGCGAGAATTTGCTCGTGATTTGCGACGACGTGAACATTCCGCTGGGGACGATCCGGATTCGGGCGAAAGGTTCGGCGGG
AGGTCAGAACGGGCTGAAACACATTATCCAGAGCCTCGGTAGCGAGGAGTTCGCGCGGCTGCGCGTCGGGGTTGGGGGGG
AGAACATGCCTGCGTCGCTCTCGTCGTTCGTGCTGTCGAAGTTTACCGCAGAAGAGCGCAAATGCATCGACAAGGTGATT
CCGGTGTGCGCCGACGCGGTACTCGACTTCGCCTCGCTGGGCGTCGAACATGCAATGACAAAATATAACGGGCAGGTCTG
TTAA

Upstream 100 bases:

>100_bases
TTTATGCAAATCATTTCTGATTCTTCATGATTTTCCCCACCCCAACATCATTCTTATATTCCGGCACCATTGAAGACACT
GAACACTTCAGATACGCGCC

Downstream 100 bases:

>100_bases
ACAGCGGCGAGTTGAGGCATGGTGTCAACGCTTTCAGGCAGAGACTGCAAGCTGACGTTACAGAGGAATTTGATCGCGCG
TGAGTTGAACTCCTGGGGCT

Product: peptidyl-tRNA hydrolase

Products: NA

Alternate protein names: PTH

Number of amino acids: Translated: 187; Mature: 187

Protein sequence:

>187_residues
MKLIVGLGNPELRHAATRHNIGFDVIDHLAGSSTFSSGKGNYRFTKITAPGGPLILVKPMTYMNLSGHAVVAAMNFWKIE
RENLLVICDDVNIPLGTIRIRAKGSAGGQNGLKHIIQSLGSEEFARLRVGVGGENMPASLSSFVLSKFTAEERKCIDKVI
PVCADAVLDFASLGVEHAMTKYNGQVC

Sequences:

>Translated_187_residues
MKLIVGLGNPELRHAATRHNIGFDVIDHLAGSSTFSSGKGNYRFTKITAPGGPLILVKPMTYMNLSGHAVVAAMNFWKIE
RENLLVICDDVNIPLGTIRIRAKGSAGGQNGLKHIIQSLGSEEFARLRVGVGGENMPASLSSFVLSKFTAEERKCIDKVI
PVCADAVLDFASLGVEHAMTKYNGQVC
>Mature_187_residues
MKLIVGLGNPELRHAATRHNIGFDVIDHLAGSSTFSSGKGNYRFTKITAPGGPLILVKPMTYMNLSGHAVVAAMNFWKIE
RENLLVICDDVNIPLGTIRIRAKGSAGGQNGLKHIIQSLGSEEFARLRVGVGGENMPASLSSFVLSKFTAEERKCIDKVI
PVCADAVLDFASLGVEHAMTKYNGQVC

Specific function: The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis

COG id: COG0193

COG function: function code J; Peptidyl-tRNA hydrolase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTH family

Homologues:

Organism=Homo sapiens, GI50897284, Length=172, Percent_Identity=30.8139534883721, Blast_Score=85, Evalue=4e-17,
Organism=Escherichia coli, GI1787455, Length=168, Percent_Identity=39.2857142857143, Blast_Score=93, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6321983, Length=187, Percent_Identity=28.3422459893048, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTH_CHLTE (Q8KD05)

Other databases:

- EMBL:   AE006470
- RefSeq:   NP_662140.1
- ProteinModelPortal:   Q8KD05
- SMR:   Q8KD05
- GeneID:   1006609
- GenomeReviews:   AE006470_GR
- KEGG:   cte:CT1252
- NMPDR:   fig|194439.1.peg.1234
- TIGR:   CT1252
- HOGENOM:   HBG610927
- OMA:   NESGRQV
- ProtClustDB:   PRK05426
- BioCyc:   CTEP194439:CT_1252-MONOMER
- BRENDA:   3.1.1.29
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00083
- InterPro:   IPR001328
- InterPro:   IPR018171
- Gene3D:   G3DSA:3.40.50.1470
- PANTHER:   PTHR17224
- TIGRFAMs:   TIGR00447

Pfam domain/function: PF01195 Pept_tRNA_hydro; SSF53178 Pept_tRNA_hydro

EC number: =3.1.1.29

Molecular weight: Translated: 20097; Mature: 20097

Theoretical pI: Translated: 8.96; Mature: 8.96

Prosite motif: PS01195 PEPT_TRNA_HYDROL_1; PS01196 PEPT_TRNA_HYDROL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLIVGLGNPELRHAATRHNIGFDVIDHLAGSSTFSSGKGNYRFTKITAPGGPLILVKPM
CEEEEECCCCHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCEEEECCC
TYMNLSGHAVVAAMNFWKIERENLLVICDDVNIPLGTIRIRAKGSAGGQNGLKHIIQSLG
EEECCCCCEEEEEEEHEEECCCCEEEEECCCCCCEEEEEEEECCCCCCHHHHHHHHHHCC
SEEFARLRVGVGGENMPASLSSFVLSKFTAEERKCIDKVIPVCADAVLDFASLGVEHAMT
CHHHEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
KYNGQVC
HCCCCCC
>Mature Secondary Structure
MKLIVGLGNPELRHAATRHNIGFDVIDHLAGSSTFSSGKGNYRFTKITAPGGPLILVKPM
CEEEEECCCCHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCEEEECCC
TYMNLSGHAVVAAMNFWKIERENLLVICDDVNIPLGTIRIRAKGSAGGQNGLKHIIQSLG
EEECCCCCEEEEEEEHEEECCCCEEEEECCCCCCEEEEEEEECCCCCCHHHHHHHHHHCC
SEEFARLRVGVGGENMPASLSSFVLSKFTAEERKCIDKVIPVCADAVLDFASLGVEHAMT
CHHHEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
KYNGQVC
HCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12093901