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The map label for this gene is rlmI [H]

Identifier: 21674038

GI number: 21674038

Start: 1138692

End: 1139876

Strand: Direct

Name: rlmI [H]

Synonym: CT1213

Alternate gene names: 21674038

Gene position: 1138692-1139876 (Clockwise)

Preceding gene: 21674036

Following gene: 21674043

Centisome position: 52.84

GC content: 57.72

Gene sequence:

>1185_bases
ATGCACTCACTCTACCTCAAACCAAAAGAACACCGGCGGCTGGTCAGCGGCCACCTGTGGGTTTTCAGCAACGAGCTTCG
GGAGGTGCCGCGTGACATTGCCGCCGGAGAAACCGTTCAGCTCTTCACCCACGACGGACGTCTGCTCGGCGCGGGGTTCT
TCAACCCCCAGTCGCTGATCGCCTTTCGCCTTCTGACGAGGGGCGAAGAGCAGCCTGACCGCGACTTTTTCCGACGCAAA
CTGCTCGAAGCGCTGAAGCTGCGCGAAAAAATCTACCCCGAAAGCGAAACCAACGCCTGGCGGCTCGTCCATGGCGAGTC
GGATGGACTACCGGGGCTGGTGATCGATCGCTTCGACCGAGCCTTCGTGCTGCAATCATTTTCCGCCGGTATCGACCAGC
ACCTGCCGCTTTTCTGCGAACTGCTCCGGGAACTGTTCGATCCTAAAGCGATCGTGGTGAGAAACGAGTCGCCGCTCAGG
GAGCTTGAGGGGCTGCCGCTCTACCGCGAAACCGTGCTCGGCGAAAGCAGCGACATGCATCAGGAGATCCGGGACAGCGG
CATCAGCTACCGGGTCAACATCCTTGAAGGCCAGAAAACCGGTTTTTTCCTCGACCAGCGCGAAAACCGGCGGCACATCC
GGAAATACGCCGCCGGCGCGGATGTACTTGACGTGTATACGAATGATGGAGGATTCGCGCTCAACGCCATGCATGCAGGT
GCAAAATCAACCACCATGGTCGATATTTCCCAGGAGGCGTTACAACGCGCCGAGCAGAACGCCCGAACGAACGGGTTCGG
AAACTTCTCGATCGTGGCTGCAGATGCATTTGAGACTCTTGGACAGCTCAGGCATGAAAACCACACTTTCGATCTGGTGA
TCCTTGATCCACCAAGCTTCACCAAAAGCCGCAAAACCGTGCCGACAGCCCTGAAAGCCTACACGAAGCTCAATCGTCTC
GGTTTGCAGCTCGTCAGGAATGAGGGATATCTGGCGACAGCCTCCTGCTCGCACCATGTTTCCGAAGAGGATTTCCTCGC
GGCCATTCACCTTGGAGCAATGCAGGCGGGCAAACATCTGCGACTTATCAGCCGCGCCGCACAACCGCCGGACCATCCGG
TGCTGCTCGCGATGCCCGAAACGAGCTATCTCAAATTTGCCTGTTTCTATGTGACAAACCTGTAG

Upstream 100 bases:

>100_bases
TCCCGTTGAATCTGAATCCCGGTCAAGGTTGATAGCTCCGAAATGTTATTTTAATTAAATCATTTCTCTGTCAAAACATG
AAACCGGGAGTTTTCGATCC

Downstream 100 bases:

>100_bases
CTAACCTAAAAAAAGCGACTTAAACATTTATCTATAGACAAAATCTGTGACATGACCCATATAAAGCGGGAGTAGTCCCG
TAAAGAATTTTCGTCATTTC

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MHSLYLKPKEHRRLVSGHLWVFSNELREVPRDIAAGETVQLFTHDGRLLGAGFFNPQSLIAFRLLTRGEEQPDRDFFRRK
LLEALKLREKIYPESETNAWRLVHGESDGLPGLVIDRFDRAFVLQSFSAGIDQHLPLFCELLRELFDPKAIVVRNESPLR
ELEGLPLYRETVLGESSDMHQEIRDSGISYRVNILEGQKTGFFLDQRENRRHIRKYAAGADVLDVYTNDGGFALNAMHAG
AKSTTMVDISQEALQRAEQNARTNGFGNFSIVAADAFETLGQLRHENHTFDLVILDPPSFTKSRKTVPTALKAYTKLNRL
GLQLVRNEGYLATASCSHHVSEEDFLAAIHLGAMQAGKHLRLISRAAQPPDHPVLLAMPETSYLKFACFYVTNL

Sequences:

>Translated_394_residues
MHSLYLKPKEHRRLVSGHLWVFSNELREVPRDIAAGETVQLFTHDGRLLGAGFFNPQSLIAFRLLTRGEEQPDRDFFRRK
LLEALKLREKIYPESETNAWRLVHGESDGLPGLVIDRFDRAFVLQSFSAGIDQHLPLFCELLRELFDPKAIVVRNESPLR
ELEGLPLYRETVLGESSDMHQEIRDSGISYRVNILEGQKTGFFLDQRENRRHIRKYAAGADVLDVYTNDGGFALNAMHAG
AKSTTMVDISQEALQRAEQNARTNGFGNFSIVAADAFETLGQLRHENHTFDLVILDPPSFTKSRKTVPTALKAYTKLNRL
GLQLVRNEGYLATASCSHHVSEEDFLAAIHLGAMQAGKHLRLISRAAQPPDHPVLLAMPETSYLKFACFYVTNL
>Mature_394_residues
MHSLYLKPKEHRRLVSGHLWVFSNELREVPRDIAAGETVQLFTHDGRLLGAGFFNPQSLIAFRLLTRGEEQPDRDFFRRK
LLEALKLREKIYPESETNAWRLVHGESDGLPGLVIDRFDRAFVLQSFSAGIDQHLPLFCELLRELFDPKAIVVRNESPLR
ELEGLPLYRETVLGESSDMHQEIRDSGISYRVNILEGQKTGFFLDQRENRRHIRKYAAGADVLDVYTNDGGFALNAMHAG
AKSTTMVDISQEALQRAEQNARTNGFGNFSIVAADAFETLGQLRHENHTFDLVILDPPSFTKSRKTVPTALKAYTKLNRL
GLQLVRNEGYLATASCSHHVSEEDFLAAIHLGAMQAGKHLRLISRAAQPPDHPVLLAMPETSYLKFACFYVTNL

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=389, Percent_Identity=34.9614395886889, Blast_Score=234, Evalue=9e-63,
Organism=Escherichia coli, GI1787181, Length=244, Percent_Identity=29.9180327868852, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 44552; Mature: 44552

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: PS00012 PHOSPHOPANTETHEINE ; PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSLYLKPKEHRRLVSGHLWVFSNELREVPRDIAAGETVQLFTHDGRLLGAGFFNPQSLI
CCCCCCCCHHHHHHHHCEEEEEEHHHHHHHHHHHCCCEEEEEEECCEEEEECCCCHHHHH
AFRLLTRGEEQPDRDFFRRKLLEALKLREKIYPESETNAWRLVHGESDGLPGLVIDRFDR
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHHHHHHH
AFVLQSFSAGIDQHLPLFCELLRELFDPKAIVVRNESPLRELEGLPLYRETVLGESSDMH
HHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHCCCCHHHHHHCCCCHHHH
QEIRDSGISYRVNILEGQKTGFFLDQRENRRHIRKYAAGADVLDVYTNDGGFALNAMHAG
HHHHHCCCEEEEEEEECCCCCEEECCCCHHHHHHHHHCCCCEEEEEECCCCEEEEEECCC
AKSTTMVDISQEALQRAEQNARTNGFGNFSIVAADAFETLGQLRHENHTFDLVILDPPSF
CCCCEEEECCHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHCCCCEEEEEEECCCCC
TKSRKTVPTALKAYTKLNRLGLQLVRNEGYLATASCSHHVSEEDFLAAIHLGAMQAGKHL
CCCCCHHHHHHHHHHHHHHHHHHHEECCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCHH
RLISRAAQPPDHPVLLAMPETSYLKFACFYVTNL
HHHHHHCCCCCCCEEEEECCCCHHEEEEEEEECC
>Mature Secondary Structure
MHSLYLKPKEHRRLVSGHLWVFSNELREVPRDIAAGETVQLFTHDGRLLGAGFFNPQSLI
CCCCCCCCHHHHHHHHCEEEEEEHHHHHHHHHHHCCCEEEEEEECCEEEEECCCCHHHHH
AFRLLTRGEEQPDRDFFRRKLLEALKLREKIYPESETNAWRLVHGESDGLPGLVIDRFDR
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHHHHHHH
AFVLQSFSAGIDQHLPLFCELLRELFDPKAIVVRNESPLRELEGLPLYRETVLGESSDMH
HHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHCCCCHHHHHHCCCCHHHH
QEIRDSGISYRVNILEGQKTGFFLDQRENRRHIRKYAAGADVLDVYTNDGGFALNAMHAG
HHHHHCCCEEEEEEEECCCCCEEECCCCHHHHHHHHHCCCCEEEEEECCCCEEEEEECCC
AKSTTMVDISQEALQRAEQNARTNGFGNFSIVAADAFETLGQLRHENHTFDLVILDPPSF
CCCCEEEECCHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHCCCCEEEEEEECCCCC
TKSRKTVPTALKAYTKLNRLGLQLVRNEGYLATASCSHHVSEEDFLAAIHLGAMQAGKHL
CCCCCHHHHHHHHHHHHHHHHHHHEECCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCHH
RLISRAAQPPDHPVLLAMPETSYLKFACFYVTNL
HHHHHHCCCCCCCEEEEECCCCHHEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA